RchiOBHm_Chr5g0037651

protein Sb04g004280 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018274
Physical Location & Seq
Forward (+)
32303874 .. 32305899
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1260 bp
ATGGTGCTATATAAGAAGGCTGCTTCATCCCAAACCCTTCATCATTCAGATTTCAGAGGCAAAACAAAACCGTTCGTTGAAGCTCCTCGAATATCTCTCTCGCTCTCAAGGTTCATGCAGATCTTTGTGAAAACCCTCACAGGCAAGACTATTACCCTCGAGGTTGAAAGCTCCGACACTATCGACAATGTCAAAGCGAAGATCCAAGACAAGGAGGGAATTCCCCCAGACCAGCAGAGGCTGATCTTTGCTGGCAAGCAGCTTGAGGACGGCCGGACGCTTGCTGACTATAACATTCAGAAGGAGTCCACTCTCCACTTGGTGCTCAGACTCCGTGGTGGCATGCAGATCTTTGTCAAGACTCTCACAGGGAAGACCATCACCTTGGAGGTCGAGAGCTCTGATACAATCGATAATGTCAAGGCAAAGATCCAGGACAAGGAGGGTATCCCCCCAGATCAGCAGAGGCTCATCTTTGCCGGCAAACAGCTAGAGGATGGTCGCACCCTTGCAGATTACAATATCCAAAAGGAGTCCACCCTTCACTTGGTGCTTCGTCTCCGTGGTGGAATGCAAATCTTTGTGAAGACCCTTACTGGGAAGACAATCACCTTGGAGGTGGAAAGCTCTGATACAATTGACAATGTCAAGGCAAAGATCCAGGACAAGGAGGGTATTCCTCCTGATCAGCAGAGGCTCATATTTGCAGGTAAACAGTTGGAGGACGGAAGAACTCTTGCAGACTATAACATTCAAAAGGAGTCGACCTTGCACTTGGTTCTCCGGCTGCGTGGTGGAATGCAAATTTTTGTGAAGACTTTGACAGGGAAAACAATTACTTTGGAAGTGGAAAGCTCGGACACAATTGATAATGTCAAGGCGAAGATTCAGGACAAAGAAGGCATTCCCCCAGACCAGCAGAGGCTCATCTTTGCAGGAAAGCAGCTTGAAGACGGTAGGACACTGGCAGACTACAACATCCAGAAGGAGTCGACTCTGCATTTGGTGCTCCGTCTACGTGGTGGGATGCAGATTTTTGTGAAGACCCTCACTGGAAAGACCATAACCTTGGAGGTGGAGAGTTCTGATACAATTGATAATGTGAAAGCAAAGATTCAGGACAAGGAGGGCATTCCTCCGGACCAGCAGAGGCTCATATTTGCTGGTAAGCAGCTTGAAGATGGGAGGACTCTAGCAGATTACAACATCCAGAAGGAGTCGACTCTTCACCTTGTCCTGCGCCTCCGTGGTGGTGTCTGA

Protein Analysis

419

Amino Acids

46.97

Weight (kDa)

8.99

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 39 - 109 9.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 41 - 112 1.3e-31 Ubiquitin family
Rad60-SLD PF11976 115 - 185 9.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 117 - 188 1.3e-31 Ubiquitin family
Rad60-SLD PF11976 191 - 261 9.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 193 - 264 1.3e-31 Ubiquitin family
Rad60-SLD PF11976 267 - 337 9.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 269 - 340 1.3e-31 Ubiquitin family
Rad60-SLD PF11976 343 - 413 9.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 345 - 416 1.3e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018274)

Species Orthologous Gene IDs
prunus_persica Prupe.2G185300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037651 RchiOBHm_Chr5g0045211
rosa_laevigata RLG00000034346
rosa_samantha Rh5AG303000 Rh5BG257400 Rh5BG311600
rosa_wichuraiana Rw5G023680 Rw5G028120 Rw5G028460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 158
Acc36I ACCTGC 1 cut(s) 698
AccI GTMKAC 4 cut(s) 764, 992, 1015, 1220
AccIII TCCGGA 1 cut(s) 1138
AclWI GGATC 3 cut(s) 196, 424, 652
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 2 cut(s) 219, 804
AdeI CACNNNGTG 2 cut(s) 322, 550
AfiI CCNNNNNNNGG 5 cut(s) 211, 439, 547, 597, 667
AgsI TTSAA 5 cut(s) 80, 167, 755, 950, 1178
AjnI CCWGG 2 cut(s) 432, 660
AluBI AGCT 9 cut(s) 83, 171, 262, 399, 490, 627, 855, 946, 1174
AluI AGCT 9 cut(s) 83, 171, 262, 399, 490, 627, 855, 946, 1174
Alw21I GWGCWC 3 cut(s) 327, 401, 1011
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 3 cut(s) 196, 424, 652
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 1138
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 5 cut(s) 20, 259, 787, 943, 1171
ApoI RAATTY 2 cut(s) 219, 804
ArsI GACNNNNNNTTYG 2 cut(s) 985, 1017
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 1 cut(s) 1242
AspS9I GGNCC 1 cut(s) 1141
AsuHPI GGTGA 3 cut(s) 373, 601, 1220
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 1 cut(s) 1141
BanII GRGCYC 1 cut(s) 401
BbsI GAAGAC 6 cut(s) 380, 593, 608, 821, 957, 1049
Bbv12I GWGCWC 3 cut(s) 327, 401, 1011
BbvI GCAGC 5 cut(s) 7, 271, 774, 955, 1183
BccI CCATC 3 cut(s) 386, 491, 1175
BceAI ACGGC 1 cut(s) 286
BciT130I CCWGG 2 cut(s) 434, 662
BciVI GTATCC 1 cut(s) 458
BclI TGATCA 1 cut(s) 685
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 2 cut(s) 491, 1193
BfuAI ACCTGC 1 cut(s) 698
BfuI GTATCC 1 cut(s) 458
BglII AGATCT 2 cut(s) 120, 348
BisI GCNGC 5 cut(s) 21, 260, 788, 944, 1172
BlsI GCNGC 5 cut(s) 22, 261, 789, 945, 1173
Bme1390I CCNGG 2 cut(s) 434, 662
Bme18I GGWCC 1 cut(s) 1141
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 1141
BmrFI CCNGG 2 cut(s) 434, 662
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 1017
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 6 cut(s) 380, 593, 608, 821, 957, 1049
BpuEI CTTGAG 2 cut(s) 91, 284
Bsa29I ATCGAT 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 1019
BsaJI CCNNGG 6 cut(s) 334, 384, 562, 612, 1068, 1246
BsaWI WCCGGW 1 cut(s) 1138
Bsc4I CCNNNNNNNGG 5 cut(s) 211, 439, 547, 597, 667
Bse118I RCCGGY 1 cut(s) 479
Bse1I ACTGG 3 cut(s) 601, 969, 1057
BseAI TCCGGA 1 cut(s) 1138
BseBI CCWGG 2 cut(s) 434, 662
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 6 cut(s) 334, 384, 562, 612, 1068, 1246
BseGI GGATG 5 cut(s) 26, 502, 978, 1032, 1206
BseLI CCNNNNNNNGG 5 cut(s) 211, 439, 547, 597, 667
BseMII CTCAG 1 cut(s) 340
BseNI ACTGG 3 cut(s) 601, 969, 1057
BseRI GAGGAG 1 cut(s) 75
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 5 cut(s) 7, 271, 774, 955, 1183
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 1 cut(s) 273
BshVI ATCGAT 1 cut(s) 411
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 3 cut(s) 327, 401, 1011
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 4 cut(s) 274, 480, 784, 1139
BslI CCNNNNNNNGG 5 cut(s) 211, 439, 547, 597, 667
BsmAI GTCTC 1 cut(s) 563
BsmBI CGTCTC 1 cut(s) 563
BsmI GAATGC 4 cut(s) 576, 804, 903, 1131
BsnI GGCC 1 cut(s) 273
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 3 cut(s) 327, 401, 1011
Bsp13I TCCGGA 1 cut(s) 1138
Bsp143I GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 1 cut(s) 339
BspDI ATCGAT 1 cut(s) 411
BspEI TCCGGA 1 cut(s) 1138
BspMI ACCTGC 1 cut(s) 698
BspPI GGATC 3 cut(s) 196, 424, 652
BsrFI RCCGGY 1 cut(s) 479
BsrI ACTGG 3 cut(s) 601, 969, 1057
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 6 cut(s) 334, 384, 562, 612, 1068, 1246
BssMI GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
BssT1I CCWWGG 3 cut(s) 384, 612, 1068
Bst2UI CCWGG 2 cut(s) 434, 662
Bst4CI ACNGT 3 cut(s) 72, 717, 956
Bst6I CTCTTC 1 cut(s) 1230
BstAPI GCANNNNNTGC 1 cut(s) 1006
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 5 cut(s) 253, 257, 282, 344, 481
BstDEI CTNAG 1 cut(s) 326
BstDSI CCRYGG 3 cut(s) 334, 562, 1246
BstF5I GGATG 5 cut(s) 26, 502, 978, 1032, 1206
BstHHI GCGC 1 cut(s) 1242
BstKTI GATC 8 cut(s) 123, 204, 246, 351, 432, 460, 660, 688
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 1 cut(s) 1006
BstNI CCWGG 2 cut(s) 434, 662
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 432, 660
BstV1I GCAGC 5 cut(s) 7, 271, 774, 955, 1183
BstV2I GAAGAC 6 cut(s) 380, 593, 608, 821, 957, 1049
BstX2I RGATCY 5 cut(s) 120, 201, 348, 429, 657
BstXI CCANNNNNNTGG 2 cut(s) 385, 1069
BstYI RGATCY 5 cut(s) 120, 201, 348, 429, 657
BstZI CGGCCG 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 411
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 1 cut(s) 273
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 3 cut(s) 334, 562, 1246
BtsCI GGATG 5 cut(s) 26, 502, 978, 1032, 1206
BtsIMutI CAGTG 2 cut(s) 962, 1050
BveI ACCTGC 1 cut(s) 698
Cac8I GCNNGC 5 cut(s) 253, 257, 282, 344, 481
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 1 cut(s) 1242
Cfr10I RCCGGY 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 1141
ClaI ATCGAT 1 cut(s) 411
CseI GACGC 1 cut(s) 286
CviAII CATG 2 cut(s) 115, 343
DdeI CTNAG 1 cut(s) 326
DpnI GATC 8 cut(s) 122, 203, 245, 350, 431, 459, 659, 687
DpnII GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
DraIII CACNNNGTG 2 cut(s) 322, 550
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 1230
EarI CTCTTC 1 cut(s) 1230
Ecl136II GAGCTC 1 cut(s) 399
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 3 cut(s) 384, 612, 1068
Eco24I GRGCYC 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 1141
Eco52I CGGCCG 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 432, 660
EcoT14I CCWWGG 3 cut(s) 384, 612, 1068
EcoT38I GRGCYC 1 cut(s) 401
ErhI CCWWGG 3 cut(s) 384, 612, 1068
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 2 cut(s) 118, 346
FaiI YATR 9 cut(s) 10, 12, 116, 291, 344, 701, 747, 1064, 1157
FatI CATG 2 cut(s) 114, 342
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 4 cut(s) 764, 992, 1015, 1220
Fnu4HI GCNGC 5 cut(s) 21, 260, 788, 944, 1172
FokI GGATG 5 cut(s) 13, 509, 965, 1039, 1193
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 5 cut(s) 21, 260, 788, 944, 1172
FspBI CTAG 2 cut(s) 491, 1193
GlaI GCGC 1 cut(s) 1241
GluI GCNGC 5 cut(s) 21, 260, 788, 944, 1172
HaeIII GGCC 1 cut(s) 273
HapII CCGG 4 cut(s) 274, 480, 784, 1139
HgaI GACGC 1 cut(s) 286
HhaI GCGC 1 cut(s) 1242
Hin1II CATG 2 cut(s) 118, 346
Hin6I GCGC 1 cut(s) 1240
HinP1I GCGC 1 cut(s) 1240
HincII GTYRAC 3 cut(s) 765, 993, 1221
HindII GTYRAC 3 cut(s) 765, 993, 1221
HpaII CCGG 4 cut(s) 274, 480, 784, 1139
HphI GGTGA 3 cut(s) 373, 601, 1220
Hpy166II GTNNAC 7 cut(s) 309, 537, 713, 765, 993, 1016, 1221
Hpy188III TCNNGA 8 cut(s) 358, 394, 683, 890, 982, 1118, 1139, 1210
Hpy8I GTNNAC 7 cut(s) 309, 537, 713, 765, 993, 1016, 1221
HpyAV CCTTC 7 cut(s) 10, 47, 295, 551, 893, 979, 1207
HpyCH4III ACNGT 3 cut(s) 72, 717, 956
HpyCH4IV ACGT 1 cut(s) 1018
HpyF10VI GCNNNNNNNGC 1 cut(s) 1006
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 1 cut(s) 1018
Hsp92II CATG 2 cut(s) 118, 346
HspAI GCGC 1 cut(s) 1240
Kpn2I TCCGGA 1 cut(s) 1138
KroI GCCGGC 1 cut(s) 479
KroNI GCCGGC 1 cut(s) 481
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
LmnI GCTCC 3 cut(s) 88, 176, 1014
Lsp1109I GCAGC 5 cut(s) 7, 271, 774, 955, 1183
LweI GCATC 1 cut(s) 1017
MaeI CTAG 2 cut(s) 491, 1193
MaeII ACGT 1 cut(s) 1018
MalI GATC 8 cut(s) 122, 203, 245, 350, 431, 459, 659, 687
MboI GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
MfeI CAATTG 3 cut(s) 636, 864, 1092
MflI RGATCY 5 cut(s) 120, 201, 348, 429, 657
MhlI GDGCHC 3 cut(s) 327, 401, 1011
MluCI AATT 6 cut(s) 219, 636, 804, 834, 864, 1092
MmeI TCCRAC 2 cut(s) 198, 699
MroI TCCGGA 1 cut(s) 1138
MroNI GCCGGC 1 cut(s) 479
MroXI GAANNNNTTC 1 cut(s) 903
MspI CCGG 4 cut(s) 274, 480, 784, 1139
MspR9I CCNGG 2 cut(s) 434, 662
MunI CAATTG 3 cut(s) 636, 864, 1092
Mva1269I GAATGC 4 cut(s) 576, 804, 903, 1131
MvaI CCWGG 2 cut(s) 434, 662
MwoI GCNNNNNNNGC 1 cut(s) 1006
NaeI GCCGGC 1 cut(s) 481
NdeII GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
NgoMIV GCCGGC 1 cut(s) 479
NlaIII CATG 2 cut(s) 118, 346
NspI RCATGY 1 cut(s) 346
PaeI GCATGC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 158
PctI GAATGC 4 cut(s) 576, 804, 903, 1131
PdiI GCCGGC 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 2 cut(s) 886, 1114
PflFI GACNNNGTC 2 cut(s) 188, 644
PfoI TCCNGGA 2 cut(s) 432, 660
PkrI GCNGC 5 cut(s) 22, 261, 789, 945, 1173
Ppu21I YACGTR 1 cut(s) 1019
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 432, 660
PspGI CCWGG 2 cut(s) 432, 660
PspPI GGNCC 1 cut(s) 1141
PspXI VCTCGAGB 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 5 cut(s) 120, 201, 348, 429, 657
PsyI GACNNNGTC 2 cut(s) 188, 644
SacI GAGCTC 1 cut(s) 401
SalI GTCGAC 3 cut(s) 763, 991, 1219
SatI GCNGC 5 cut(s) 21, 260, 788, 944, 1172
Sau3AI GATC 8 cut(s) 120, 201, 243, 348, 429, 457, 657, 685
Sau96I GGNCC 1 cut(s) 1141
ScrFI CCNGG 2 cut(s) 434, 662
SduI GDGCHC 3 cut(s) 327, 401, 1011
SfaNI GCATC 1 cut(s) 1017
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 1 cut(s) 1141
SlaI CTCGAG 1 cut(s) 158
SmlI CTYRAG 3 cut(s) 106, 158, 263
SmoI CTYRAG 3 cut(s) 106, 158, 263
SphI GCATGC 1 cut(s) 346
Sse9I AATT 6 cut(s) 219, 636, 804, 834, 864, 1092
SspMI CTAG 2 cut(s) 491, 1193
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 432, 660
StyI CCWWGG 3 cut(s) 384, 612, 1068
TaaI ACNGT 3 cut(s) 72, 717, 956
TaiI ACGT 1 cut(s) 1021
TaqI TCGA 8 cut(s) 88, 159, 183, 393, 411, 764, 992, 1220
TasI AATT 6 cut(s) 219, 636, 804, 834, 864, 1092
TfiI GAWTC 2 cut(s) 886, 1114
TscAI CASTG 2 cut(s) 969, 1057
TseI GCWGC 5 cut(s) 20, 259, 787, 943, 1171
TspDTI ATGAA 3 cut(s) 15, 29, 103
TspGWI ACGGA 5 cut(s) 323, 551, 741, 1001, 1235
TspRI CASTG 2 cut(s) 969, 1057
Tth111I GACNNNGTC 2 cut(s) 188, 644
VpaK11BI GGWCC 1 cut(s) 1141
XapI RAATTY 2 cut(s) 219, 804
XceI RCATGY 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 2 cut(s) 316, 544
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 4 cut(s) 764, 992, 1015, 1220
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 491, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.