RchiOBHm_Chr5g0078361

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0025041
Physical Location & Seq
Forward (+)
84265031 .. 84265393
363 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGGCCTTTATCACCGACGCCCTAGGCCAAGTATATCAATTGGCTCCTTTTCTTCTAGGTTTAATCATACCAGATGGACCACCACTTGGTGCAACATTAACAGCCAAGACCGAATTCTTGGTCTCAGAACTTCTTCTGCCAGTGTTCTTTTTTCTTATTGGCAACAGCGTAAATGTGTTTTTGATTGACGATTGGACTAAGTTTGCCAAACTTCAGATTATTATTCTCGTGTTATTTGTTACAAAGATAGTGGGAGTTACAGTGGCTGGCTTGTACTGCAAAATTAGCTTGAAAAATAGTCTCAGCCTCAGCATGATCATGAGTATATATGAAGGGTATAATAGAGCTTATTATTTATCATAA

Protein Analysis

120

Amino Acids

13.26

Weight (kDa)

6.08

Isoelectric Point (pI)

19.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 2 - 108 3e-12 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0025041)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0078361
rosa_roxburghii Rroxscaffold_4G00285950
rosa_samantha Rh5DG548800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 86
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 1 cut(s) 292
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 2 cut(s) 288, 347
AluI AGCT 2 cut(s) 288, 347
Alw26I GTCTC 2 cut(s) 127, 305
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 2 cut(s) 3, 25
ApoI RAATTY 1 cut(s) 113
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
Asp700I GAANNNNTTC 1 cut(s) 132
AspA2I CCTAGG 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 4
AvaII GGWCC 1 cut(s) 77
AvrII CCTAGG 1 cut(s) 22
BauI CACGAG 1 cut(s) 227
BbvCI CCTCAGC 1 cut(s) 308
BccI CCATC 1 cut(s) 68
BclI TGATCA 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 127, 305
BfaI CTAG 2 cut(s) 23, 56
BlnI CCTAGG 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 45
Bpu10I CCTNAGC 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 22
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 3 cut(s) 138, 316, 322
BseNI ACTGG 1 cut(s) 140
BshFI GGCC 2 cut(s) 5, 27
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 2 cut(s) 127, 305
BsnI GGCC 2 cut(s) 5, 27
Bso31I GGTCTC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 315
BspANI GGCC 2 cut(s) 5, 27
BspCNI CTCAG 3 cut(s) 137, 315, 321
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 127
BsrI ACTGG 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 315
BssNI GRCGYC 1 cut(s) 18
BssSI CACGAG 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 262
BstACI GRCGYC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 4 cut(s) 124, 198, 302, 308
BstKTI GATC 1 cut(s) 318
BstMAI GTCTC 2 cut(s) 127, 305
BstMBI GATC 1 cut(s) 315
BstMWI GCNNNNNNNGC 2 cut(s) 276, 285
BsuRI GGCC 2 cut(s) 5, 27
BtsIMutI CAGTG 2 cut(s) 147, 267
Cac8I GCNNGC 1 cut(s) 268
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 77
CseI GACGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 274
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 2 cut(s) 313, 319
CviJI RGCY 9 cut(s) 5, 27, 44, 104, 266, 270, 288, 306, 347
CviKI_1 RGCY 9 cut(s) 5, 27, 44, 104, 266, 270, 288, 306, 347
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 4 cut(s) 124, 198, 302, 308
DpnI GATC 1 cut(s) 317
DpnII GATC 1 cut(s) 315
DraIII CACNNNGTG 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 2 cut(s) 316, 322
FaiI YATR 9 cut(s) 34, 68, 314, 320, 326, 328, 330, 339, 361
FatI CATG 2 cut(s) 312, 318
FbaI TGATCA 1 cut(s) 315
FspBI CTAG 2 cut(s) 23, 56
HaeIII GGCC 2 cut(s) 5, 27
HgaI GACGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 2 cut(s) 316, 322
HphI GGTGA 1 cut(s) 4
Hpy188I TCNGA 2 cut(s) 127, 216
Hpy188III TCNNGA 1 cut(s) 319
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4V TGCA 2 cut(s) 92, 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 285
HpyF3I CTNAG 4 cut(s) 124, 198, 302, 308
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 2 cut(s) 316, 322
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 1 cut(s) 315
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 3 cut(s) 84, 153, 252
MaeI CTAG 2 cut(s) 23, 56
MaeIII GTNAC 2 cut(s) 238, 256
MalI GATC 1 cut(s) 317
MboI GATC 1 cut(s) 315
MboII GAAGA 2 cut(s) 44, 125
MfeI CAATTG 1 cut(s) 38
MluCI AATT 3 cut(s) 38, 113, 282
MnlI CCTC 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 132
MseI TTAA 2 cut(s) 62, 98
MslI CAYNNNNRTG 1 cut(s) 317
MunI CAATTG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 276, 285
NdeII GATC 1 cut(s) 315
NlaIII CATG 2 cut(s) 316, 322
NlaIV GGNNCC 1 cut(s) 45
PagI TCATGA 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 132
PflMI CCANNNNNTGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 1 cut(s) 77
PstNI CAGNNNCTG 1 cut(s) 266
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 2 cut(s) 62, 98
Sau3AI GATC 1 cut(s) 315
Sau96I GGNCC 1 cut(s) 77
SetI ASST 3 cut(s) 61, 290, 349
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 3 cut(s) 38, 113, 282
SspMI CTAG 2 cut(s) 23, 56
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 1 cut(s) 262
TaqII GACCGA 1 cut(s) 125
TasI AATT 3 cut(s) 38, 113, 282
TatI WGTACW 1 cut(s) 273
Tru1I TTAA 2 cut(s) 62, 98
Tru9I TTAA 2 cut(s) 62, 98
TscAI CASTG 2 cut(s) 147, 267
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 2 cut(s) 147, 267
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 1 cut(s) 113
XmaJI CCTAGG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 2 cut(s) 23, 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.