Rh5DG548800

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0025041
Physical Location & Seq
Forward (+)
84403676 .. 84404407
732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG548800.1

Sequence Viewer

Length: 732 bp
ATGTCCTCTGCAATGCTTAATGATGCCGCATTATGGTTCCTCATTATACTTAATGTGATTGTTTCAACCAAAGGATATCCAGTATTGCTTTCCACGTTGGGGTTGCTACTCTTCACAATCTATATCATACGCCCACTTATGCTGTTGATTGTGAAGAAAACACCTGAAGGAGAAGAAGTAAAGGAAATCTATATAGTTCCGATACTTCTTGGGGTTCTAGTCATGGCCTTTATCACCGACGCCCTAGGCCAAGTATATCAATTGGCTCCTTTTCTTCTAGGTTTAATCATACCAGATGGACCACCACTTGGTGCAACATTAACAGCCAAGACCGATTTCTTGGTCTTAGAACTTCTTCTGCCAGTGTTCTTTTTTCTTATTGGCAACAGCGTAAATGTGTTTTTGATTGACGATTGGACTAAGTTTTCCAAACTTCAGATTATTATTCTTGTGTTATTTGTTACAAAGATAGTGGGAGTTTCAGTGGCTGGCTTGTGCTGCAAAATTAGCTTGAAAAATAGTCTCATCCTCAGCATGATCATGAGTATGAAGGGTATAATAGAGCTTATGATTTATCATAAATGGATGACCATGAAGGTAATGGATATAGACTTTTTTCTCTCTCTTTTTCCTCCCTTTCATTTCTATATATACTTGCACCACTATGGTAAGGGATTTGCATTATTCCATCTTACAGAGTCATTGTACGTGCTCTCTAATGCTCATGTTTAG

Protein Analysis

243

Amino Acids

27.32

Weight (kDa)

7.01

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 3 - 196 2.8e-16 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0025041)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0078361
rosa_roxburghii Rroxscaffold_4G00285950
rosa_samantha Rh5DG548800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AciI CCGC 1 cut(s) 27
AcuI CTGAAG 2 cut(s) 186, 419
AcyI GRCGYC 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 311
AfaI GTAC 1 cut(s) 707
AfiI CCNNNNNNNGG 3 cut(s) 33, 99, 308
AgsI TTSAA 2 cut(s) 66, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 2 cut(s) 510, 565
AluI AGCT 2 cut(s) 510, 565
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 1 cut(s) 527
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 2 cut(s) 225, 247
ApeKI GCWGC 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 354
AspA2I CCTAGG 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 1 cut(s) 299
AvrII CCTAGG 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 1 cut(s) 485
BccI CCATC 2 cut(s) 290, 696
BclI TGATCA 1 cut(s) 537
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 3 cut(s) 218, 245, 278
BisI GCNGC 2 cut(s) 27, 499
BlnI CCTAGG 1 cut(s) 244
BlsI GCNGC 2 cut(s) 28, 500
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 38, 267
BmsI GCATC 1 cut(s) 13
Bpu10I CCTNAGC 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 709
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 468, 498
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 99, 308
Bse1I ACTGG 2 cut(s) 80, 362
Bse3DI GCAATG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 2 cut(s) 525, 591
BseLI CCNNNNNNNGG 3 cut(s) 33, 99, 308
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 544
BseNI ACTGG 2 cut(s) 80, 362
BseXI GCAGC 1 cut(s) 485
BshFI GGCC 2 cut(s) 227, 249
BsiHKAI GWGCWC 1 cut(s) 714
BslI CCNNNNNNNGG 3 cut(s) 33, 99, 308
BsmAI GTCTC 1 cut(s) 527
BsnI GGCC 2 cut(s) 227, 249
Bsp1286I GDGCHC 1 cut(s) 714
Bsp143I GATC 1 cut(s) 537
BspACI CCGC 1 cut(s) 27
BspANI GGCC 2 cut(s) 227, 249
BspCNI CTCAG 1 cut(s) 543
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 2 cut(s) 38, 267
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 80, 362
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 1 cut(s) 537
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 240
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 3 cut(s) 346, 420, 530
BstF5I GGATG 2 cut(s) 525, 591
BstKTI GATC 1 cut(s) 540
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 1 cut(s) 537
BstMWI GCNNNNNNNGC 2 cut(s) 498, 507
BstV1I GCAGC 1 cut(s) 485
BsuRI GGCC 2 cut(s) 227, 249
BtsCI GGATG 2 cut(s) 525, 591
BtsIMutI CAGTG 2 cut(s) 369, 489
Cac8I GCNNGC 1 cut(s) 490
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 540
Cfr13I GGNCC 1 cut(s) 299
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 706
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 5 cut(s) 223, 535, 541, 592, 725
CviJI RGCY 8 cut(s) 227, 249, 266, 326, 488, 492, 510, 565
CviKI_1 RGCY 8 cut(s) 227, 249, 266, 326, 488, 492, 510, 565
CviQI GTAC 1 cut(s) 706
DdeI CTNAG 3 cut(s) 346, 420, 530
DpnI GATC 1 cut(s) 539
DpnII GATC 1 cut(s) 537
DraIII CACNNNGTG 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 244
Eco32I GATATC 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 186, 419
EcoRV GATATC 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 5 cut(s) 226, 538, 544, 595, 728
FatI CATG 5 cut(s) 222, 534, 540, 591, 724
FbaI TGATCA 1 cut(s) 537
Fnu4HI GCNGC 2 cut(s) 27, 499
FokI GGATG 2 cut(s) 512, 598
Fsp4HI GCNGC 2 cut(s) 27, 499
FspBI CTAG 3 cut(s) 218, 245, 278
GluI GCNGC 2 cut(s) 27, 499
HaeIII GGCC 2 cut(s) 227, 249
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 5 cut(s) 226, 538, 544, 595, 728
HinfI GANTC 1 cut(s) 698
HphI GGTGA 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 201, 438
Hpy188III TCNNGA 1 cut(s) 541
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 161, 544, 589
HpyCH4IV ACGT 2 cut(s) 95, 708
HpyCH4V TGCA 5 cut(s) 11, 314, 501, 658, 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 498, 507
HpyF3I CTNAG 3 cut(s) 346, 420, 530
HpySE526I ACGT 2 cut(s) 95, 708
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 5 cut(s) 226, 538, 544, 595, 728
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 1 cut(s) 537
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 5 cut(s) 93, 177, 306, 375, 474
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 218, 245, 278
MaeII ACGT 2 cut(s) 95, 708
MaeIII GTNAC 1 cut(s) 460
MalI GATC 1 cut(s) 539
MboI GATC 1 cut(s) 537
MboII GAAGA 5 cut(s) 103, 166, 185, 266, 347
MfeI CAATTG 1 cut(s) 260
MhlI GDGCHC 1 cut(s) 714
MluCI AATT 2 cut(s) 260, 504
MlyI GAGTC 1 cut(s) 707
MnlI CCTC 4 cut(s) 16, 50, 539, 642
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 4 cut(s) 18, 51, 284, 320
MslI CAYNNNNRTG 3 cut(s) 539, 545, 663
MunI CAATTG 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 498, 507
NdeII GATC 1 cut(s) 537
NlaIII CATG 5 cut(s) 226, 538, 544, 595, 728
NlaIV GGNNCC 2 cut(s) 38, 267
PagI TCATGA 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 354
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 2 cut(s) 28, 500
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
Ppu21I YACGTR 1 cut(s) 709
PspN4I GGNNCC 2 cut(s) 38, 267
PspPI GGNCC 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 488
RsaI GTAC 1 cut(s) 707
RsaNI GTAC 1 cut(s) 706
RseI CAYNNNNRTG 3 cut(s) 539, 545, 663
SaqAI TTAA 4 cut(s) 18, 51, 284, 320
SatI GCNGC 2 cut(s) 27, 499
Sau3AI GATC 1 cut(s) 537
Sau96I GGNCC 1 cut(s) 299
SchI GAGTC 1 cut(s) 707
SduI GDGCHC 1 cut(s) 714
SetI ASST 7 cut(s) 98, 166, 283, 512, 567, 600, 711
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 3 cut(s) 539, 545, 663
Sse9I AATT 2 cut(s) 260, 504
SsiI CCGC 1 cut(s) 27
SspMI CTAG 3 cut(s) 218, 245, 278
StyI CCWWGG 1 cut(s) 244
TaiI ACGT 2 cut(s) 98, 711
TaqII GACCGA 1 cut(s) 347
TasI AATT 2 cut(s) 260, 504
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 18, 51, 284, 320
Tru9I TTAA 4 cut(s) 18, 51, 284, 320
TscAI CASTG 2 cut(s) 369, 489
TseI GCWGC 1 cut(s) 498
TspDTI ATGAA 3 cut(s) 563, 608, 629
TspRI CASTG 2 cut(s) 369, 489
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XmaJI CCTAGG 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 3 cut(s) 218, 245, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.