Rroxscaffold_4G00285950

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0025041
Physical Location & Seq
Reverse (-)
7134942 .. 7135573
632 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00285950.1

Sequence Viewer

Length: 576 bp
ATGGCCTTGTATGAACTTGATCTTATGACTTCAGAGTTGGGCCAACTAGCACTATCCTCTACAATGCTTAATGATGCCGCACTATGGTTCCTCGTTATAGTTAATATGATTGCTTTTAGCAATGGAATTCTGGCATTCCTTTCCACGTTGGGGTTGCTACTCTTCACATTCTACATCTTACACCCACTTATGCTGTTGATTGTTAAGAAAACACTCGAAGGAAAAGCAGTAAAGGAAATATATATAGTTGCGTTACTGCTTGGGGTTCTAGTCATGGCCTTTATCACTGACGCCCTAGGCCAAAGGTATAGTTTCGCTCCTTTTATTCTAGGTTTAATCATACCAAATGGACCACCACTTGGTGCAACATTAACGGCCAAGACCGAACTCCTGGTCTCGGAAGTTCTTCTACCAGTGTTCTTTTTTCGTCTTGGCAGCAGCGTAAATGTGTTTATGATTGACGATTGGACTAGATTTGCAAAAATTCAGATTATTATTCTTGCGCTTGAAGAGTTGTTTCGTACTCAGCATGATCATGAGCATGAAGGGTATAGTAGAGTTTATGATCTACAGTAG

Protein Analysis

191

Amino Acids

21.41

Weight (kDa)

5.09

Isoelectric Point (pI)

26.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 5 - 168 2.8e-12 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025041)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0078361
rosa_roxburghii Rroxscaffold_4G00285950
rosa_samantha Rh5DG548800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 359
AciI CCGC 1 cut(s) 78
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 2 cut(s) 126, 483
AcuI CTGAAG 1 cut(s) 15
AcyI GRCGYC 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 362
AfaI GTAC 1 cut(s) 523
AfiI CCNNNNNNNGG 4 cut(s) 84, 150, 359, 397
AgsI TTSAA 1 cut(s) 509
AjnI CCWGG 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 400
AoxI GGCC 5 cut(s) 3, 40, 276, 298, 375
ApeKI GCWGC 2 cut(s) 435, 438
ApoI RAATTY 2 cut(s) 126, 483
ArsI GACNNNNNNTTYG 2 cut(s) 378, 410
Asp700I GAANNNNTTC 1 cut(s) 405
AspA2I CCTAGG 1 cut(s) 295
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 2 cut(s) 40, 350
AvaII GGWCC 1 cut(s) 350
AvrII CCTAGG 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 393, 425
BbvI GCAGC 2 cut(s) 447, 450
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 1 cut(s) 392
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 5 cut(s) 47, 269, 296, 329, 471
BfmI CTRYAG 1 cut(s) 569
BisI GCNGC 3 cut(s) 78, 436, 439
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 3 cut(s) 79, 437, 440
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 40, 350
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 295
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 150, 359, 397
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 127
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 1 cut(s) 295
BseLI CCNNNNNNNGG 4 cut(s) 84, 150, 359, 397
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 447, 450
BshFI GGCC 5 cut(s) 5, 42, 278, 300, 377
BslI CCNNNNNNNGG 4 cut(s) 84, 150, 359, 397
BsmAI GTCTC 1 cut(s) 400
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 5 cut(s) 5, 42, 278, 300, 377
Bso31I GGTCTC 1 cut(s) 400
Bsp143I GATC 3 cut(s) 19, 532, 565
BspACI CCGC 1 cut(s) 78
BspANI GGCC 5 cut(s) 5, 42, 278, 300, 377
BspCNI CTCAG 1 cut(s) 538
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 3 cut(s) 19, 532, 565
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 1 cut(s) 573
Bst6I CTCTTC 2 cut(s) 167, 504
BstACI GRCGYC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 525
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 3 cut(s) 22, 535, 568
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 3 cut(s) 19, 532, 565
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 569
BstV1I GCAGC 2 cut(s) 447, 450
BsuRI GGCC 5 cut(s) 5, 42, 278, 300, 377
BtsIMutI CAGTG 2 cut(s) 285, 420
CciI TCATGA 1 cut(s) 535
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 2 cut(s) 40, 350
CseI GACGC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 522
CviAII CATG 4 cut(s) 274, 530, 536, 542
CviJI RGCY 5 cut(s) 5, 42, 278, 300, 377
CviKI_1 RGCY 5 cut(s) 5, 42, 278, 300, 377
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 1 cut(s) 525
DpnI GATC 3 cut(s) 21, 534, 567
DpnII GATC 3 cut(s) 19, 532, 565
DraIII CACNNNGTG 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 2 cut(s) 167, 504
EarI CTCTTC 2 cut(s) 167, 504
Eco130I CCWWGG 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 4 cut(s) 277, 533, 539, 545
FatI CATG 4 cut(s) 273, 529, 535, 541
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 78, 436, 439
Fsp4HI GCNGC 3 cut(s) 78, 436, 439
FspBI CTAG 5 cut(s) 47, 269, 296, 329, 471
GlaI GCGC 1 cut(s) 504
GluI GCNGC 3 cut(s) 78, 436, 439
HaeIII GGCC 5 cut(s) 5, 42, 278, 300, 377
HgaI GACGC 1 cut(s) 299
HhaI GCGC 1 cut(s) 505
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 4 cut(s) 277, 533, 539, 545
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
Hpy188I TCNGA 3 cut(s) 34, 400, 489
Hpy188III TCNNGA 1 cut(s) 536
HpyAV CCTTC 2 cut(s) 212, 539
HpyCH4III ACNGT 1 cut(s) 573
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 2 cut(s) 365, 479
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 4 cut(s) 277, 533, 539, 545
HspAI GCGC 1 cut(s) 503
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 3 cut(s) 19, 532, 565
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 4 cut(s) 116, 377, 404, 426
Lsp1109I GCAGC 2 cut(s) 447, 450
LweI GCATC 1 cut(s) 64
MaeI CTAG 5 cut(s) 47, 269, 296, 329, 471
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 1 cut(s) 252
MalI GATC 3 cut(s) 21, 534, 567
MboI GATC 3 cut(s) 19, 532, 565
MboII GAAGA 3 cut(s) 154, 398, 521
MluCI AATT 2 cut(s) 126, 483
MnlI CCTC 2 cut(s) 67, 101
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 5 cut(s) 69, 102, 204, 335, 371
MslI CAYNNNNRTG 2 cut(s) 534, 540
MspR9I CCNGG 1 cut(s) 392
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 1 cut(s) 392
NdeII GATC 3 cut(s) 19, 532, 565
NlaIII CATG 4 cut(s) 277, 533, 539, 545
NlaIV GGNNCC 1 cut(s) 89
PagI TCATGA 1 cut(s) 535
PctI GAATGC 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 405
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 3 cut(s) 79, 437, 440
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 2 cut(s) 40, 350
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
RseI CAYNNNNRTG 2 cut(s) 534, 540
SaqAI TTAA 5 cut(s) 69, 102, 204, 335, 371
SatI GCNGC 3 cut(s) 78, 436, 439
Sau3AI GATC 3 cut(s) 19, 532, 565
Sau96I GGNCC 2 cut(s) 40, 350
ScrFI CCNGG 1 cut(s) 392
SetI ASST 3 cut(s) 149, 308, 334
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 569
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 2 cut(s) 534, 540
Sse9I AATT 2 cut(s) 126, 483
SsiI CCGC 1 cut(s) 78
SspMI CTAG 5 cut(s) 47, 269, 296, 329, 471
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 1 cut(s) 573
TaiI ACGT 1 cut(s) 149
TaqI TCGA 1 cut(s) 216
TaqII GACCGA 1 cut(s) 398
TasI AATT 2 cut(s) 126, 483
TauI GCSGC 1 cut(s) 80
Tru1I TTAA 5 cut(s) 69, 102, 204, 335, 371
Tru9I TTAA 5 cut(s) 69, 102, 204, 335, 371
TscAI CASTG 2 cut(s) 292, 420
TseI GCWGC 2 cut(s) 435, 438
TspDTI ATGAA 2 cut(s) 27, 558
TspRI CASTG 2 cut(s) 292, 420
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 2 cut(s) 126, 483
XmaJI CCTAGG 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 5 cut(s) 47, 269, 296, 329, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.