RchiOBHm_Chr4g0432491

Multidrug Resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0022730
Physical Location & Seq
Forward (+)
56655968 .. 56659299
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 996 bp
ATGAGACTCTTTATAACTCTAGCATATTGGTATGGAGGGAGGCTAATGAACAAAGGCACCATAACTGCAGAGCACGTGTTTCAAACTTTCTTCTTATTGATGAGCACTGGTAAAAGCATTGCAGGTGCCGGAAGTATAACCTCTGATTTGGCCAAAGGCTACAATGCCATAGCATCAGTGTTTTCTATCCTAGACAGGTCAAGTGAGATTCAGCCTGATGAAGGCAATAAGCTGCAAAAGACTATTAAAGGCCACATAGAACTGAAGAATGTGTTTTTCTCTTACCCAGCAAGGCCAGAACAAATGATCTTCAAGGGCTTGAATCTCACGATTGAAGCTGGAAGAACAATGGAATTAGTGGGACATAGTGTTTCAGGTAATACCATTGTCATTGGACTAGTCGAAAGATTTTACGACCCTGTAAAGGGATCAGTACATATAGACCAACACAATATTAAGAACTACAACTTGAGGCAACTGAGGTATCGCAGTGGTGAGTCAGGAGCCTACAACAATCTGTGGTCTGGAGGGATGAGATGGAAGTTTAGCGTGGTTGCTGCTACGGTGTCGATCTGCGGTTCTGGGGTTCCGTCCTCTTCATGGTGTAGCGCCGACCCGGTGGTAGTCGCTCGAAACCTGTGGGGTCGGGGTTGGGGTTTGGGGATCAGGTTGTCTTGGATTGGGGTTGTGGGTATTGCCCCCGGATCTGGTTGGGATAGGGTGGTCGAAATAGCTGGGCTTTGGGACAATCGGGTTGGTGGGGCGCTGACAGAGGTGATCGTCAGCAGGATAGCAATTGCTCGAGCAATACTGAAGAACCTGAAAGTTCTTCTGTTGGATGAAGCAACCACTGTCCTAGATAGTGTGTCGGAGACTCTAGTCCAAGAAGCGCTTGGCAAGATGATGGTAGGCAGAACATGTGTCGTGGTAGCTCATCGCTTGTCCACGATGCAAAGGGAGAGTGGGAGACACCATCTCTGTTATAGAGAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

331

Amino Acids

36.43

Weight (kDa)

9.53

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 105 - 164 1.9e-06 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022730)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0432491
rosa_rugosa Rorug04G0259800
rosa_samantha Rh4DG318200
rosa_wichuraiana Rw4G027350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 14
AarI CACCTGC 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 113
AccB1I GGYRCC 2 cut(s) 56, 125
AciI CCGC 1 cut(s) 576
AclWI GGATC 3 cut(s) 436, 671, 712
AcoI YGGCCR 1 cut(s) 150
AcuI CTGAAG 2 cut(s) 284, 833
AcvI CACGTG 1 cut(s) 76
AfaI GTAC 1 cut(s) 435
AfeI AGCGCT 1 cut(s) 891
AfiI CCNNNNNNNGG 5 cut(s) 221, 424, 425, 600, 707
AflIII ACRYGT 2 cut(s) 75, 917
AgsI TTSAA 4 cut(s) 83, 313, 322, 335
AhlI ACTAGT 1 cut(s) 397
AluBI AGCT 4 cut(s) 232, 338, 734, 932
AluI AGCT 4 cut(s) 232, 338, 734, 932
Alw21I GWGCWC 2 cut(s) 75, 107
Alw26I GTCTC 2 cut(s) 866, 961
AlwI GGATC 3 cut(s) 436, 671, 712
Ama87I CYCGRG 1 cut(s) 801
Aor51HI AGCGCT 1 cut(s) 891
AoxI GGCC 3 cut(s) 150, 250, 293
ApeKI GCWGC 2 cut(s) 232, 557
AspLEI GCGC 3 cut(s) 611, 766, 892
AsuC2I CCSGG 2 cut(s) 617, 702
AsuHPI GGTGA 2 cut(s) 506, 787
AvaI CYCGRG 1 cut(s) 801
BaeI ACNNNNGTAYC 2 cut(s) 467, 500
BalI TGGCCA 1 cut(s) 152
BanI GGYRCC 2 cut(s) 56, 125
BbrPI CACGTG 1 cut(s) 76
Bbv12I GWGCWC 2 cut(s) 75, 107
BbvI GCAGC 2 cut(s) 219, 544
BccI CCATC 3 cut(s) 531, 898, 981
BcnI CCSGG 2 cut(s) 617, 702
BcoDI GTCTC 2 cut(s) 866, 961
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 5 cut(s) 20, 191, 398, 857, 878
BfmI CTRYAG 1 cut(s) 66
BfoI RGCGCY 3 cut(s) 612, 767, 893
BfuAI ACCTGC 1 cut(s) 113
BisI GCNGC 2 cut(s) 233, 558
BlsI GCNGC 2 cut(s) 234, 559
Bme1390I CCNGG 2 cut(s) 617, 702
BmeT110I CYCGRG 1 cut(s) 801
BmiI GGNNCC 4 cut(s) 58, 127, 505, 588
BmrFI CCNGG 2 cut(s) 617, 702
BmsI GCATC 2 cut(s) 182, 939
BoxI GACNNNNGTC 1 cut(s) 878
BpmI CTGGAG 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 490
BpuMI CCSGG 2 cut(s) 617, 702
BsaAI YACGTR 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 700
Bsc4I CCNNNNNNNGG 5 cut(s) 221, 424, 425, 600, 707
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 700
BseGI GGATG 2 cut(s) 537, 844
BseLI CCNNNNNNNGG 5 cut(s) 221, 424, 425, 600, 707
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 219, 544
BseYI CCCAGC 2 cut(s) 286, 734
BshFI GGCC 3 cut(s) 152, 252, 295
BshNI GGYRCC 2 cut(s) 56, 125
BsiHKAI GWGCWC 2 cut(s) 75, 107
BsiHKCI CYCGRG 1 cut(s) 801
BsiSI CCGG 3 cut(s) 129, 617, 702
BslFI GGGAC 2 cut(s) 375, 758
BslI CCNNNNNNNGG 5 cut(s) 221, 424, 425, 600, 707
BsmAI GTCTC 2 cut(s) 866, 961
BsmFI GGGAC 2 cut(s) 375, 758
BsnI GGCC 3 cut(s) 152, 252, 295
BsoBI CYCGRG 1 cut(s) 801
Bsp1286I GDGCHC 2 cut(s) 75, 107
Bsp143I GATC 6 cut(s) 306, 428, 570, 663, 704, 777
BspACI CCGC 1 cut(s) 576
BspANI GGCC 3 cut(s) 152, 252, 295
BspCNI CTCAG 1 cut(s) 471
BspLI GGNNCC 4 cut(s) 58, 127, 505, 588
BspMAI CTGCAG 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 113
BspPI GGATC 3 cut(s) 436, 671, 712
BspT107I GGYRCC 2 cut(s) 56, 125
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 700
BssMI GATC 6 cut(s) 306, 428, 570, 663, 704, 777
Bst4CI ACNGT 2 cut(s) 565, 853
Bst6I CTCTTC 1 cut(s) 601
BstBAI YACGTR 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 479
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 537, 844
BstH2I RGCGCY 3 cut(s) 612, 767, 893
BstHHI GCGC 3 cut(s) 611, 766, 892
BstKTI GATC 6 cut(s) 309, 431, 573, 666, 707, 780
BstMAI GTCTC 2 cut(s) 866, 961
BstMBI GATC 6 cut(s) 306, 428, 570, 663, 704, 777
BstNSI RCATGY 1 cut(s) 921
BstPAI GACNNNNGTC 1 cut(s) 878
BstSCI CCNGG 2 cut(s) 615, 700
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 219, 544
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
BsuRI GGCC 3 cut(s) 152, 252, 295
BtgZI GCGATG 1 cut(s) 920
BtsCI GGATG 2 cut(s) 537, 844
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 4 cut(s) 105, 183, 496, 849
BveI ACCTGC 1 cut(s) 113
CfoI GCGC 3 cut(s) 611, 766, 892
Csp6I GTAC 1 cut(s) 434
CviAII CATG 2 cut(s) 600, 918
CviQI GTAC 1 cut(s) 434
DdeI CTNAG 1 cut(s) 479
DpnI GATC 6 cut(s) 308, 430, 572, 665, 706, 779
DpnII GATC 6 cut(s) 306, 428, 570, 663, 704, 777
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 601
EarI CTCTTC 1 cut(s) 601
Eco47III AGCGCT 1 cut(s) 891
Eco57I CTGAAG 2 cut(s) 284, 833
Eco72I CACGTG 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 801
EcoNI CCTNNNNNAGG 1 cut(s) 219
FaeI CATG 2 cut(s) 603, 921
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 2 cut(s) 375, 758
FatI CATG 2 cut(s) 599, 917
Fnu4HI GCNGC 2 cut(s) 233, 558
FokI GGATG 2 cut(s) 544, 851
Fsp4HI GCNGC 2 cut(s) 233, 558
FspBI CTAG 5 cut(s) 20, 191, 398, 857, 878
GlaI GCGC 3 cut(s) 610, 765, 891
GluI GCNGC 2 cut(s) 233, 558
GsaI CCCAGC 2 cut(s) 290, 738
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 3 cut(s) 612, 767, 893
HaeIII GGCC 3 cut(s) 152, 252, 295
HapII CCGG 3 cut(s) 129, 617, 702
HhaI GCGC 3 cut(s) 611, 766, 892
Hin1II CATG 2 cut(s) 603, 921
Hin6I GCGC 3 cut(s) 609, 764, 890
HinP1I GCGC 3 cut(s) 609, 764, 890
HinfI GANTC 5 cut(s) 6, 208, 322, 497, 874
HpaII CCGG 3 cut(s) 129, 617, 702
HphI GGTGA 2 cut(s) 506, 787
Hpy166II GTNNAC 1 cut(s) 945
Hpy188I TCNGA 2 cut(s) 145, 871
Hpy188III TCNNGA 3 cut(s) 328, 501, 525
Hpy8I GTNNAC 1 cut(s) 945
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 2 cut(s) 565, 853
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 4 cut(s) 68, 122, 235, 952
HpyF3I CTNAG 1 cut(s) 479
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 2 cut(s) 603, 921
HspAI GCGC 3 cut(s) 609, 764, 890
Kzo9I GATC 6 cut(s) 306, 428, 570, 663, 704, 777
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 2 cut(s) 219, 544
LweI GCATC 2 cut(s) 182, 939
MaeI CTAG 5 cut(s) 20, 191, 398, 857, 878
MaeII ACGT 1 cut(s) 75
MalI GATC 6 cut(s) 308, 430, 572, 665, 706, 779
MboI GATC 6 cut(s) 306, 428, 570, 663, 704, 777
MboII GAAGA 7 cut(s) 82, 277, 301, 354, 588, 821, 826
MfeI CAATTG 1 cut(s) 795
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 2 cut(s) 75, 107
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 2 cut(s) 353, 795
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 2 cut(s) 506, 868
MmeI TCCRAC 2 cut(s) 816, 849
MnlI CCTC 8 cut(s) 29, 33, 151, 465, 474, 521, 604, 766
Mox20I TGGCCA 1 cut(s) 152
MscI TGGCCA 1 cut(s) 152
MseI TTAA 2 cut(s) 246, 456
Msp20I TGGCCA 1 cut(s) 152
MspI CCGG 3 cut(s) 129, 617, 702
MspR9I CCNGG 2 cut(s) 617, 702
MunI CAATTG 1 cut(s) 795
NciI CCSGG 2 cut(s) 617, 702
NdeII GATC 6 cut(s) 306, 428, 570, 663, 704, 777
NlaIII CATG 2 cut(s) 603, 921
NlaIV GGNNCC 4 cut(s) 58, 127, 505, 588
NspI RCATGY 1 cut(s) 921
PaeR7I CTCGAG 1 cut(s) 801
PaqCI CACCTGC 1 cut(s) 113
PciI ACATGT 1 cut(s) 917
PfeI GAWTC 2 cut(s) 208, 322
PkrI GCNGC 2 cut(s) 234, 559
PleI GAGTC 2 cut(s) 505, 868
PmaCI CACGTG 1 cut(s) 76
PmlI CACGTG 1 cut(s) 76
PpsI GAGTC 2 cut(s) 505, 868
Ppu21I YACGTR 1 cut(s) 76
PscI ACATGT 1 cut(s) 917
PshAI GACNNNNGTC 1 cut(s) 878
PsiI TTATAA 1 cut(s) 14
PspCI CACGTG 1 cut(s) 76
PspFI CCCAGC 2 cut(s) 286, 734
PspN4I GGNNCC 4 cut(s) 58, 127, 505, 588
PspXI VCTCGAGB 1 cut(s) 801
PstI CTGCAG 1 cut(s) 70
PsuI RGATCY 1 cut(s) 704
RsaI GTAC 1 cut(s) 435
RsaNI GTAC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 246, 456
SatI GCNGC 2 cut(s) 233, 558
Sau3AI GATC 6 cut(s) 306, 428, 570, 663, 704, 777
SchI GAGTC 2 cut(s) 506, 868
ScrFI CCNGG 2 cut(s) 617, 702
SduI GDGCHC 2 cut(s) 75, 107
SfaNI GCATC 2 cut(s) 182, 939
SfcI CTRYAG 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 801
SlaI CTCGAG 1 cut(s) 801
SmlI CTYRAG 2 cut(s) 469, 801
SmoI CTYRAG 2 cut(s) 469, 801
SpeI ACTAGT 1 cut(s) 397
Sse9I AATT 2 cut(s) 353, 795
SsiI CCGC 1 cut(s) 576
SspI AATATT 1 cut(s) 454
SspMI CTAG 5 cut(s) 20, 191, 398, 857, 878
StyD4I CCNGG 2 cut(s) 615, 700
TaaI ACNGT 2 cut(s) 565, 853
TaiI ACGT 1 cut(s) 78
TaqI TCGA 5 cut(s) 402, 569, 631, 726, 802
TasI AATT 2 cut(s) 353, 795
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 2 cut(s) 208, 322
Tru1I TTAA 2 cut(s) 246, 456
Tru9I TTAA 2 cut(s) 246, 456
TscAI CASTG 4 cut(s) 112, 183, 496, 856
TseI GCWGC 2 cut(s) 232, 557
TspDTI ATGAA 4 cut(s) 62, 234, 588, 855
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 4 cut(s) 112, 183, 496, 856
XagI CCTNNNNNAGG 1 cut(s) 219
XceI RCATGY 1 cut(s) 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 890
XhoI CTCGAG 1 cut(s) 801
XspI CTAG 5 cut(s) 20, 191, 398, 857, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.