Rh4DG318200

Multidrug Resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0022730
Physical Location & Seq
Forward (+)
54107221 .. 54107742
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG318200.1

Sequence Viewer

Length: 522 bp
ATGAGACTCTTTATAACTCTAGCATATTGGTATGGAGGGAGGCTAATGAACAAAGGCTCCATAACTGCAGAGCACGTGTTTCAAACTTTCTTCTTATTGATGAGCACTGGTAAAAGCATTGCAGGTGCCGGAAGTATAACCTCTGATTTGGCCAAAGGCTACAATGCCATAGCATCAGTGTTTTCTATCCTAGACAGGTCAAGTGAGATTCAGCCTGATGAAGGCAATAAGCTGCAAAAGACTATTAAAGGCCACATAGAACTGAAGAATGTGTTTTTCTCTTACCCAGCAAGGCCAGAACAAATGATCTTCAAGGGCTTGAATCTCACGATTGAAGCTGGAAGAACAATGGAATTAGTGGGACATAGTGTTTCAGGTAATACCATTGTCATTGGACTAGTCGAAAGATTTTACGACCCTGTAAAGGGATCAGTACATATAGACCAACACAATATTAAGAACTACAACTTGAGGCAACTGAGGTATCGCAGTGGTGAGTCAGGAGCCTACAACAATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

173

Amino Acids

19.31

Weight (kDa)

9.45

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 105 - 162 8.3e-07 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022730)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0432491
rosa_rugosa Rorug04G0259800
rosa_samantha Rh4DG318200
rosa_wichuraiana Rw4G027350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 14
AarI CACCTGC 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 125
AclWI GGATC 1 cut(s) 436
AcoI YGGCCR 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 284
AcvI CACGTG 1 cut(s) 76
AfaI GTAC 1 cut(s) 435
AfiI CCNNNNNNNGG 3 cut(s) 221, 424, 425
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 4 cut(s) 83, 313, 322, 335
AhlI ACTAGT 1 cut(s) 397
AloI GAACNNNNNNTCC 2 cut(s) 41, 73
AluBI AGCT 2 cut(s) 232, 338
AluI AGCT 2 cut(s) 232, 338
Alw21I GWGCWC 2 cut(s) 75, 107
AlwI GGATC 1 cut(s) 436
AoxI GGCC 3 cut(s) 150, 250, 293
ApeKI GCWGC 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 506
BaeI ACNNNNGTAYC 2 cut(s) 467, 500
BalI TGGCCA 1 cut(s) 152
BanI GGYRCC 1 cut(s) 125
BbrPI CACGTG 1 cut(s) 76
Bbv12I GWGCWC 2 cut(s) 75, 107
BbvI GCAGC 1 cut(s) 219
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 3 cut(s) 20, 191, 398
BfmI CTRYAG 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 113
BisI GCNGC 1 cut(s) 233
BlsI GCNGC 1 cut(s) 234
BmiI GGNNCC 3 cut(s) 58, 127, 505
BmsI GCATC 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 490
BsaAI YACGTR 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 424, 425
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 117
BseLI CCNNNNNNNGG 3 cut(s) 221, 424, 425
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 219
BseYI CCCAGC 1 cut(s) 286
BshFI GGCC 3 cut(s) 152, 252, 295
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 75, 107
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 3 cut(s) 221, 424, 425
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 3 cut(s) 152, 252, 295
Bsp1286I GDGCHC 2 cut(s) 75, 107
Bsp143I GATC 2 cut(s) 306, 428
BspANI GGCC 3 cut(s) 152, 252, 295
BspCNI CTCAG 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 58, 127, 505
BspMAI CTGCAG 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 113
BspPI GGATC 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 1 cut(s) 112
BssMI GATC 2 cut(s) 306, 428
BstBAI YACGTR 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 479
BstENI CCTNNNNNAGG 1 cut(s) 219
BstKTI GATC 2 cut(s) 309, 431
BstMBI GATC 2 cut(s) 306, 428
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 219
BsuRI GGCC 3 cut(s) 152, 252, 295
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 3 cut(s) 105, 183, 496
BveI ACCTGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 434
CviQI GTAC 1 cut(s) 434
DdeI CTNAG 1 cut(s) 479
DpnI GATC 2 cut(s) 308, 430
DpnII GATC 2 cut(s) 306, 428
EaeI YGGCCR 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 284
Eco72I CACGTG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 219
FaqI GGGAC 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 233
FspBI CTAG 3 cut(s) 20, 191, 398
GluI GCNGC 1 cut(s) 233
GsaI CCCAGC 1 cut(s) 290
HaeIII GGCC 3 cut(s) 152, 252, 295
HapII CCGG 1 cut(s) 129
HinfI GANTC 4 cut(s) 6, 208, 322, 497
HpaII CCGG 1 cut(s) 129
HphI GGTGA 1 cut(s) 506
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 2 cut(s) 328, 501
HpyAV CCTTC 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 3 cut(s) 68, 122, 235
HpyF3I CTNAG 1 cut(s) 479
HpySE526I ACGT 1 cut(s) 75
Kzo9I GATC 2 cut(s) 306, 428
LmnI GCTCC 2 cut(s) 62, 503
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 1 cut(s) 182
MaeI CTAG 3 cut(s) 20, 191, 398
MaeII ACGT 1 cut(s) 75
MalI GATC 2 cut(s) 308, 430
MboI GATC 2 cut(s) 306, 428
MboII GAAGA 4 cut(s) 82, 277, 301, 354
MhlI GDGCHC 2 cut(s) 75, 107
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 1 cut(s) 353
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 1 cut(s) 506
MnlI CCTC 5 cut(s) 29, 33, 151, 465, 474
Mox20I TGGCCA 1 cut(s) 152
MscI TGGCCA 1 cut(s) 152
MseI TTAA 2 cut(s) 246, 456
Msp20I TGGCCA 1 cut(s) 152
MspI CCGG 1 cut(s) 129
NdeII GATC 2 cut(s) 306, 428
NlaIV GGNNCC 3 cut(s) 58, 127, 505
PaqCI CACCTGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 208, 322
PkrI GCNGC 1 cut(s) 234
PleI GAGTC 1 cut(s) 505
PmaCI CACGTG 1 cut(s) 76
PmlI CACGTG 1 cut(s) 76
PpsI GAGTC 1 cut(s) 505
Ppu21I YACGTR 1 cut(s) 76
PsiI TTATAA 1 cut(s) 14
PspCI CACGTG 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 286
PspN4I GGNNCC 3 cut(s) 58, 127, 505
PstI CTGCAG 1 cut(s) 70
RsaI GTAC 1 cut(s) 435
RsaNI GTAC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 246, 456
SatI GCNGC 1 cut(s) 233
Sau3AI GATC 2 cut(s) 306, 428
SchI GAGTC 1 cut(s) 506
SduI GDGCHC 2 cut(s) 75, 107
SetI ASST 8 cut(s) 78, 127, 143, 200, 234, 340, 379, 485
SfaNI GCATC 1 cut(s) 182
SfcI CTRYAG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SpeI ACTAGT 1 cut(s) 397
Sse9I AATT 1 cut(s) 353
SspI AATATT 1 cut(s) 454
SspMI CTAG 3 cut(s) 20, 191, 398
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 402
TasI AATT 1 cut(s) 353
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 2 cut(s) 208, 322
Tru1I TTAA 2 cut(s) 246, 456
Tru9I TTAA 2 cut(s) 246, 456
TscAI CASTG 3 cut(s) 112, 183, 496
TseI GCWGC 1 cut(s) 232
TspDTI ATGAA 2 cut(s) 62, 234
TspRI CASTG 3 cut(s) 112, 183, 496
XagI CCTNNNNNAGG 1 cut(s) 219
XspI CTAG 3 cut(s) 20, 191, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.