Rw4G027350

Multidrug resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0022730
Physical Location & Seq
Forward (+)
53807424 .. 53811870
4447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027350.1

Sequence Viewer

Length: 1071 bp
ATGAAAGGTTTGTCCAAGAAAGTGCAAAAAGCACAAATTAAGAGCAGTCAACAAGCAAGTGAAGCCACCATCAACCATAGAAATATCACTGAATCTTCGTCTCAGAAAAGAATAATGAGACTCTTTATAACTCTAGCATATTGGTATGGAGGGAGGCTAATGAACAAAAGCTCCATAACTGCAGAGCACGTGTTTCAAACTTTCTTCTTATTGATGAGCACTGGTAAAAGCATTGCAGGTGCCGGAAGTATGACCTCTGATTTGGCCAGAGGCTACAATGCCATAGCATCAGTGTTTTCTATCCTAGACAGGTCAAGTGAGATTCAGCCTGATGAAGGCAATAAGGTGCAAAAGACTATTAAAGGCCACATAGAACTGAAGAATGTGTTTTTCTCTTACCCAGCAAGGCCAGAACAAATGATCTTCAAGGGCTTGAATCTCACGATTGAAGCTGGAAGAACAATGGCATTAGTGGGACATAGTGTTTCAGGTAATACCATTGTCATTGGACTAGTCGAAAGATTTTACGACCCTGTAAAGGGATCAGTACATATAGACCAACACAATATTAAGAACTACAACTTGAGGCAACTGAGGTCCTATATCGCAGTGGTGAGTCAGGAGCCTACAACAATCTGTGAAAATATCCTTTATGGTAAAGAGAATTTTAGGGAATCTGAAGTAAGAAGGCCAGCAATTCTTTTGAATGCTCATGAATTTATAAGAACCCAAATCCTTCCCTCCTATGAACCATGTCAAGAATTTGACAACCCATGTCAAGAATTTGATATCAAACACGTCTTCTCTTCTCAATACCCAGAAGAATTGCACAACCCAGTTCTCATTCCGGATTCGTTCCCGCCGGAGACCCAAAATAATATCCTTCGTGGTCGCCGCCGCTCTCCAGCTCCGACTCCGTCGCTCTCCACCACTTACACCCTCTCTGGAAAGGTAACAGACAAAACAAGCTTCAGGAAATTTGTAGTTGAGAGCATATTTTGGCTGATGCTTTATGCAAGTCCTGCTGCATATTGTTATTCTAGTACTTGTGGAGGTCTACTTTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

356

Amino Acids

40.11

Weight (kDa)

9.22

Isoelectric Point (pI)

59.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 143 - 214 1.5e-10 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022730)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0432491
rosa_rugosa Rorug04G0259800
rosa_samantha Rh4DG318200
rosa_wichuraiana Rw4G027350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 128, 722
AarI CACCTGC 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 239
AccBSI CCGCTC 1 cut(s) 900
AccI GTMKAC 1 cut(s) 1057
AccIII TCCGGA 1 cut(s) 847
AciI CCGC 3 cut(s) 860, 895, 898
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 5 cut(s) 664, 716, 761, 782, 977
AcuI CTGAAG 3 cut(s) 398, 699, 955
AcvI CACGTG 1 cut(s) 190
AfaI GTAC 2 cut(s) 549, 1045
AfiI CCNNNNNNNGG 3 cut(s) 335, 538, 539
AflIII ACRYGT 2 cut(s) 189, 796
AgsI TTSAA 5 cut(s) 197, 427, 436, 449, 706
AhlI ACTAGT 1 cut(s) 511
AjiI CACGTC 1 cut(s) 799
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 4 cut(s) 171, 452, 908, 969
AluI AGCT 4 cut(s) 171, 452, 908, 969
Alw21I GWGCWC 2 cut(s) 189, 221
Alw26I GTCTC 3 cut(s) 105, 112, 860
AlwI GGATC 1 cut(s) 550
Aor13HI TCCGGA 1 cut(s) 847
AoxI GGCC 4 cut(s) 264, 364, 407, 689
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 5 cut(s) 664, 716, 761, 782, 977
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
AspS9I GGNCC 1 cut(s) 597
AsuHPI GGTGA 1 cut(s) 625
AvaII GGWCC 1 cut(s) 597
BalI TGGCCA 1 cut(s) 266
BanI GGYRCC 1 cut(s) 239
BbrPI CACGTG 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 2 cut(s) 189, 221
BbvI GCAGC 1 cut(s) 1012
BccI CCATC 1 cut(s) 77
BcoDI GTCTC 3 cut(s) 105, 112, 860
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 4 cut(s) 134, 305, 512, 1041
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 227
BisI GCNGC 3 cut(s) 895, 898, 1026
BlsI GCNGC 3 cut(s) 896, 899, 1027
BmcAI AGTACT 1 cut(s) 1045
Bme18I GGWCC 1 cut(s) 597
BmgBI CACGTC 1 cut(s) 799
BmgT120I GGNCC 1 cut(s) 597
BmiI GGNNCC 2 cut(s) 241, 624
BmrI ACTGGG 1 cut(s) 830
BmsI GCATC 2 cut(s) 296, 996
BmuI ACTGGG 1 cut(s) 830
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 604
BsaAI YACGTR 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 860
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 538, 539
Bse1I ACTGG 2 cut(s) 226, 836
Bse3DI GCAATG 1 cut(s) 231
BseAI TCCGGA 1 cut(s) 847
BseLI CCNNNNNNNGG 3 cut(s) 335, 538, 539
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 2 cut(s) 116, 584
BseNI ACTGG 2 cut(s) 226, 836
BseXI GCAGC 1 cut(s) 1012
BseYI CCCAGC 1 cut(s) 400
BshFI GGCC 4 cut(s) 266, 366, 409, 691
BshNI GGYRCC 1 cut(s) 239
BsiHKAI GWGCWC 2 cut(s) 189, 221
BsiSI CCGG 3 cut(s) 243, 848, 863
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 3 cut(s) 335, 538, 539
BsmAI GTCTC 3 cut(s) 105, 112, 860
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 489
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 4 cut(s) 266, 366, 409, 691
Bso31I GGTCTC 1 cut(s) 860
Bsp1286I GDGCHC 2 cut(s) 189, 221
Bsp13I TCCGGA 1 cut(s) 847
Bsp143I GATC 2 cut(s) 420, 542
BspACI CCGC 3 cut(s) 860, 895, 898
BspANI GGCC 4 cut(s) 266, 366, 409, 691
BspCNI CTCAG 2 cut(s) 115, 585
BspEI TCCGGA 1 cut(s) 847
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 2 cut(s) 241, 624
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 1 cut(s) 550
BspT107I GGYRCC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 860
BsrBI CCGCTC 1 cut(s) 900
BsrDI GCAATG 1 cut(s) 231
BsrI ACTGG 2 cut(s) 226, 836
BssMI GATC 2 cut(s) 420, 542
Bst6I CTCTTC 1 cut(s) 811
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 3 cut(s) 102, 593, 1068
BstENI CCTNNNNNAGG 1 cut(s) 333
BstKTI GATC 2 cut(s) 423, 545
BstMAI GTCTC 3 cut(s) 105, 112, 860
BstMBI GATC 2 cut(s) 420, 542
BstMWI GCNNNNNNNGC 2 cut(s) 62, 1022
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 1012
BstV2I GAAGAC 1 cut(s) 793
BsuRI GGCC 4 cut(s) 266, 366, 409, 691
BtrI CACGTC 1 cut(s) 799
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 4 cut(s) 87, 219, 297, 615
BveI ACCTGC 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 693
CciI TCATGA 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 597
Csp6I GTAC 2 cut(s) 548, 1044
CviAII CATG 3 cut(s) 713, 753, 774
CviQI GTAC 2 cut(s) 548, 1044
DdeI CTNAG 3 cut(s) 102, 593, 1068
DpnI GATC 2 cut(s) 422, 544
DpnII GATC 2 cut(s) 420, 542
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 811
Eco31I GGTCTC 1 cut(s) 860
Eco32I GATATC 1 cut(s) 790
Eco47I GGWCC 1 cut(s) 597
Eco57I CTGAAG 3 cut(s) 398, 699, 955
Eco72I CACGTG 1 cut(s) 190
EcoNI CCTNNNNNAGG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 597
EcoRV GATATC 1 cut(s) 790
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 3 cut(s) 716, 756, 777
FaqI GGGAC 1 cut(s) 489
FatI CATG 3 cut(s) 712, 752, 773
FauI CCCGC 1 cut(s) 867
FblI GTMKAC 1 cut(s) 1057
Fnu4HI GCNGC 3 cut(s) 895, 898, 1026
Fsp4HI GCNGC 3 cut(s) 895, 898, 1026
FspBI CTAG 4 cut(s) 134, 305, 512, 1041
GluI GCNGC 3 cut(s) 895, 898, 1026
GsaI CCCAGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 4 cut(s) 266, 366, 409, 691
HapII CCGG 3 cut(s) 243, 848, 863
Hin1II CATG 3 cut(s) 716, 756, 777
HincII GTYRAC 1 cut(s) 50
HindII GTYRAC 1 cut(s) 50
HindIII AAGCTT 1 cut(s) 967
HinfI GANTC 8 cut(s) 92, 120, 322, 436, 616, 674, 851, 913
HpaII CCGG 3 cut(s) 243, 848, 863
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 2 cut(s) 50, 1058
Hpy188I TCNGA 4 cut(s) 105, 259, 679, 912
Hpy188III TCNNGA 8 cut(s) 442, 620, 713, 758, 779, 848, 945, 973
Hpy8I GTNNAC 2 cut(s) 50, 1058
Hpy99I CGWCG 1 cut(s) 922
HpyAV CCTTC 4 cut(s) 329, 681, 746, 893
HpyCH4IV ACGT 2 cut(s) 189, 798
HpyCH4V TGCA 7 cut(s) 25, 182, 236, 349, 829, 1016, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 1022
HpyF3I CTNAG 3 cut(s) 102, 593, 1068
HpySE526I ACGT 2 cut(s) 189, 798
Hsp92II CATG 3 cut(s) 716, 756, 777
Kpn2I TCCGGA 1 cut(s) 847
Kzo9I GATC 2 cut(s) 420, 542
LmnI GCTCC 3 cut(s) 176, 622, 913
Lsp1109I GCAGC 1 cut(s) 1012
LweI GCATC 2 cut(s) 296, 996
MaeI CTAG 4 cut(s) 134, 305, 512, 1041
MaeII ACGT 2 cut(s) 189, 798
MaeIII GTNAC 1 cut(s) 952
MalI GATC 2 cut(s) 422, 544
MbiI CCGCTC 1 cut(s) 900
MboI GATC 2 cut(s) 420, 542
MboII GAAGA 8 cut(s) 87, 196, 391, 415, 468, 793, 798, 833
MhlI GDGCHC 2 cut(s) 189, 221
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 8 cut(s) 36, 664, 696, 716, 761, 782, 824, 977
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 3 cut(s) 114, 625, 907
MmeI TCCRAC 1 cut(s) 935
MnlI CCTC 9 cut(s) 143, 147, 263, 265, 579, 588, 751, 950, 1046
Mox20I TGGCCA 1 cut(s) 266
MroI TCCGGA 1 cut(s) 847
MscI TGGCCA 1 cut(s) 266
MseI TTAA 3 cut(s) 39, 360, 570
Msp20I TGGCCA 1 cut(s) 266
MspI CCGG 3 cut(s) 243, 848, 863
Mva1269I GAATGC 1 cut(s) 712
MwoI GCNNNNNNNGC 2 cut(s) 62, 1022
NdeII GATC 2 cut(s) 420, 542
NlaIII CATG 3 cut(s) 716, 756, 777
NlaIV GGNNCC 2 cut(s) 241, 624
PagI TCATGA 1 cut(s) 712
PaqCI CACCTGC 1 cut(s) 227
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 5 cut(s) 92, 322, 436, 674, 851
PflFI GACNNNGTC 1 cut(s) 916
PkrI GCNGC 3 cut(s) 896, 899, 1027
PleI GAGTC 3 cut(s) 114, 624, 907
PmaCI CACGTG 1 cut(s) 190
PmlI CACGTG 1 cut(s) 190
PpsI GAGTC 3 cut(s) 114, 624, 907
Ppu21I YACGTR 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 597
PsiI TTATAA 2 cut(s) 128, 722
Psp5II RGGWCCY 1 cut(s) 597
PspCI CACGTG 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 241, 624
PspPI GGNCC 1 cut(s) 597
PspPPI RGGWCCY 1 cut(s) 597
PstI CTGCAG 1 cut(s) 184
PsyI GACNNNGTC 1 cut(s) 916
RsaI GTAC 2 cut(s) 549, 1045
RsaNI GTAC 2 cut(s) 548, 1044
SaqAI TTAA 3 cut(s) 39, 360, 570
SatI GCNGC 3 cut(s) 895, 898, 1026
Sau3AI GATC 2 cut(s) 420, 542
Sau96I GGNCC 1 cut(s) 597
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 3 cut(s) 114, 625, 907
SduI GDGCHC 2 cut(s) 189, 221
SfaNI GCATC 2 cut(s) 296, 996
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
SpeI ACTAGT 1 cut(s) 511
Sse9I AATT 8 cut(s) 36, 664, 696, 716, 761, 782, 824, 977
SsiI CCGC 3 cut(s) 860, 895, 898
SspI AATATT 1 cut(s) 568
SspMI CTAG 4 cut(s) 134, 305, 512, 1041
TaiI ACGT 2 cut(s) 192, 801
TaqI TCGA 1 cut(s) 516
TasI AATT 8 cut(s) 36, 664, 696, 716, 761, 782, 824, 977
TatI WGTACW 2 cut(s) 547, 1043
TauI GCSGC 2 cut(s) 897, 900
TfiI GAWTC 5 cut(s) 92, 322, 436, 674, 851
Tru1I TTAA 3 cut(s) 39, 360, 570
Tru9I TTAA 3 cut(s) 39, 360, 570
TscAI CASTG 4 cut(s) 94, 226, 297, 615
TseI GCWGC 1 cut(s) 1025
TspDTI ATGAA 5 cut(s) 17, 176, 348, 729, 762
TspGWI ACGGA 1 cut(s) 906
TspRI CASTG 4 cut(s) 94, 226, 297, 615
Tth111I GACNNNGTC 1 cut(s) 916
VpaK11BI GGWCC 1 cut(s) 597
XagI CCTNNNNNAGG 1 cut(s) 333
XapI RAATTY 5 cut(s) 664, 716, 761, 782, 977
XmiI GTMKAC 1 cut(s) 1057
XspI CTAG 4 cut(s) 134, 305, 512, 1041
ZrmI AGTACT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.