RchiOBHm_Chr3g0451691

Subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0024921
Physical Location & Seq
Forward (+)
2673993 .. 2674793
801 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGGGCATGGCAGGCATCTCAATTTTCTTGACATTTGTGGTTCTCTTCCTTACTAGTACTCTAGCTAGCACTGATCAGACGAGCAACAGCAGCCGAGTTTATATTGTGTACGTGGGATCTGCAGCTCCAAACTCTTTAACCAGCACTACAGCTTCCTTGAGGACTGACCATGCTCGCCTCCTTACTTCTCTGTCGAGACGGAAAGCAAATGCGTTGGTGCACATTTACAGGCATGGTTTCGCAGGATTCGCGGCTCGGCTATCAGAGGAGGAGGCACAGTCGATGGCTCAAAAACCTGGAACTGAGTTAGAAATCGACTCATTTCCTAACAGCATCTCCGAGAATGATGATGGTGCTGCTGATGACACAGCACCGGACTCTAATGGATCTGACACCATTATCAGCATTTTAGACACTGGTATTTGGCCGGAGTCGGAGAGTTTTAATGACAAGGATATGGGTCCGATTCCATCAAGGTGGAAGGGAACATGCATGAAAGGACCTGATTTTATTACTTCATCCAGCTGTAATAGGTAA

Protein Analysis

178

Amino Acids

19.11

Weight (kDa)

4.84

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 100 2.2e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024921)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0451691
rosa_samantha Rh6BG467500 Rh6CG448000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 251
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 124, 394
AcoI YGGCCR 1 cut(s) 425
AfaI GTAC 2 cut(s) 58, 110
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 4 cut(s) 65, 125, 152, 525
AluI AGCT 4 cut(s) 65, 125, 152, 525
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 190
Alw44I GTGCAC 1 cut(s) 218
AlwI GGATC 2 cut(s) 124, 394
AoxI GGCC 1 cut(s) 425
ApaLI GTGCAC 1 cut(s) 218
ApeKI GCWGC 3 cut(s) 90, 122, 356
AspS9I GGNCC 2 cut(s) 461, 500
AsuNHI GCTAGC 1 cut(s) 65
AvaII GGWCC 2 cut(s) 461, 500
BaeGI GKGCMC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 102, 134, 343
BccI CCATC 3 cut(s) 277, 344, 478
BciT130I CCWGG 1 cut(s) 297
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 190
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 3 cut(s) 54, 62, 66
BfmI CTRYAG 2 cut(s) 120, 147
BisI GCNGC 4 cut(s) 91, 123, 252, 357
BlsI GCNGC 4 cut(s) 92, 124, 253, 358
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 2 cut(s) 461, 500
BmgT120I GGNCC 2 cut(s) 461, 500
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 2 cut(s) 24, 342
BmtI GCTAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 320, 350
Bse1I ACTGG 1 cut(s) 421
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 518
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 2 cut(s) 281, 284
BseSI GKGCMC 1 cut(s) 222
BseXI GCAGC 3 cut(s) 102, 134, 343
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 2 cut(s) 374, 428
BsmAI GTCTC 1 cut(s) 190
BsmBI CGTCTC 1 cut(s) 190
BsnI GGCC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 3 cut(s) 73, 116, 386
BspACI CCGC 1 cut(s) 251
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 1 cut(s) 251
BspLI GGNNCC 1 cut(s) 462
BspMAI CTGCAG 1 cut(s) 124
BspOI GCTAGC 1 cut(s) 69
BspPI GGATC 2 cut(s) 124, 394
BsrI ACTGG 1 cut(s) 421
BssMI GATC 3 cut(s) 73, 116, 386
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 279
Bst6I CTCTTC 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 112
BstC8I GCNNGC 3 cut(s) 13, 67, 175
BstDEI CTNAG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 518
BstFNI CGCG 1 cut(s) 251
BstKTI GATC 3 cut(s) 76, 119, 389
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 3 cut(s) 73, 116, 386
BstMWI GCNNNNNNNGC 3 cut(s) 12, 90, 248
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 2 cut(s) 120, 147
BstSLI GKGCMC 1 cut(s) 222
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 3 cut(s) 102, 134, 343
BstX2I RGATCY 2 cut(s) 116, 386
BstXI CCANNNNNNTGG 1 cut(s) 477
BstYI RGATCY 2 cut(s) 116, 386
BsuRI GGCC 1 cut(s) 427
BtsCI GGATG 1 cut(s) 518
BtsIMutI CAGTG 2 cut(s) 69, 414
Cac8I GCNNGC 3 cut(s) 13, 67, 175
Cfr13I GGNCC 2 cut(s) 461, 500
Csp6I GTAC 2 cut(s) 57, 109
CviAII CATG 5 cut(s) 7, 170, 233, 489, 493
CviJI RGCY 9 cut(s) 65, 93, 125, 152, 254, 259, 287, 427, 525
CviKI_1 RGCY 9 cut(s) 65, 93, 125, 152, 254, 259, 287, 427, 525
CviQI GTAC 2 cut(s) 57, 109
DdeI CTNAG 1 cut(s) 303
DpnI GATC 3 cut(s) 75, 118, 388
DpnII GATC 3 cut(s) 73, 116, 386
EaeI YGGCCR 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 461, 500
EcoO109I RGGNCCY 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 295
EcoT22I ATGCAT 1 cut(s) 494
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 5 cut(s) 10, 173, 236, 492, 496
FaiI YATR 7 cut(s) 8, 102, 171, 234, 458, 490, 494
FatI CATG 5 cut(s) 6, 169, 232, 488, 492
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 4 cut(s) 91, 123, 252, 357
FokI GGATG 1 cut(s) 505
Fsp4HI GCNGC 4 cut(s) 91, 123, 252, 357
FspBI CTAG 3 cut(s) 54, 62, 66
GluI GCNGC 4 cut(s) 91, 123, 252, 357
HaeIII GGCC 1 cut(s) 427
HapII CCGG 2 cut(s) 374, 428
Hin1II CATG 5 cut(s) 10, 173, 236, 492, 496
HinfI GANTC 5 cut(s) 246, 317, 377, 431, 466
HpaII CCGG 2 cut(s) 374, 428
Hpy166II GTNNAC 2 cut(s) 109, 220
Hpy188I TCNGA 6 cut(s) 78, 265, 340, 391, 436, 465
Hpy188III TCNNGA 2 cut(s) 28, 195
Hpy8I GTNNAC 2 cut(s) 109, 220
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 122, 220, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 90, 248
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 5 cut(s) 10, 173, 236, 492, 496
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 73, 116, 386
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 9 cut(s) 154, 214, 228, 282, 309, 387, 402, 441, 516
Lsp1109I GCAGC 3 cut(s) 102, 134, 343
LweI GCATC 2 cut(s) 24, 342
MaeI CTAG 3 cut(s) 54, 62, 66
MaeII ACGT 1 cut(s) 111
MalI GATC 3 cut(s) 75, 118, 388
MboI GATC 3 cut(s) 73, 116, 386
MboII GAAGA 1 cut(s) 37
MflI RGATCY 2 cut(s) 116, 386
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 1 cut(s) 21
MlyI GAGTC 3 cut(s) 311, 371, 440
MmeI TCCRAC 1 cut(s) 414
MnlI CCTC 5 cut(s) 153, 188, 259, 262, 265
Mph1103I ATGCAT 1 cut(s) 494
MseI TTAA 2 cut(s) 137, 444
MslI CAYNNNNRTG 1 cut(s) 475
MspA1I CMGCKG 1 cut(s) 525
MspI CCGG 2 cut(s) 374, 428
MspR9I CCNGG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 297
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 3 cut(s) 12, 90, 248
NdeII GATC 3 cut(s) 73, 116, 386
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 5 cut(s) 10, 173, 236, 492, 496
NlaIV GGNNCC 1 cut(s) 462
NmeAIII GCCGAG 2 cut(s) 119, 235
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 1 cut(s) 492
PfeI GAWTC 2 cut(s) 246, 466
PkrI GCNGC 4 cut(s) 92, 124, 253, 358
PleI GAGTC 3 cut(s) 311, 371, 439
PpsI GAGTC 3 cut(s) 311, 371, 439
Ppu21I YACGTR 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 2 cut(s) 461, 500
PspPPI RGGWCCY 1 cut(s) 500
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 2 cut(s) 116, 386
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 2 cut(s) 58, 110
RsaNI GTAC 2 cut(s) 57, 109
RseI CAYNNNNRTG 1 cut(s) 475
SaqAI TTAA 2 cut(s) 137, 444
SatI GCNGC 4 cut(s) 91, 123, 252, 357
Sau3AI GATC 3 cut(s) 73, 116, 386
Sau96I GGNCC 2 cut(s) 461, 500
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 3 cut(s) 311, 371, 440
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 222
SetI ASST 9 cut(s) 67, 114, 127, 154, 298, 479, 505, 527, 536
SfaNI GCATC 2 cut(s) 24, 342
SfcI CTRYAG 2 cut(s) 120, 147
SinI GGWCC 2 cut(s) 461, 500
SmiMI CAYNNNNRTG 1 cut(s) 475
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 1 cut(s) 21
SsiI CCGC 1 cut(s) 251
SspMI CTAG 3 cut(s) 54, 62, 66
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 1 cut(s) 279
TaiI ACGT 1 cut(s) 114
TaqI TCGA 3 cut(s) 194, 281, 315
TasI AATT 1 cut(s) 21
TatI WGTACW 1 cut(s) 56
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 246, 466
Tru1I TTAA 2 cut(s) 137, 444
Tru9I TTAA 2 cut(s) 137, 444
TscAI CASTG 2 cut(s) 76, 421
TseI GCWGC 3 cut(s) 90, 122, 356
TspDTI ATGAA 2 cut(s) 507, 509
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 2 cut(s) 76, 421
VneI GTGCAC 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 461, 500
XceI RCATGY 1 cut(s) 492
XspI CTAG 3 cut(s) 54, 62, 66
ZrmI AGTACT 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.