RchiOBHm_Chr1g0340371

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0019333
Physical Location & Seq
Reverse (-)
31963469 .. 31971723
8255 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 288 bp
ATGCTGATTAGAGCCCCAGATCTAGTGGCCTTGGAATATGCTGACCTTGATCTCACCCACAATGTTTCTCAGGTCGGTGCCAGTAGAAGTACCTTATTGGAATCAAGTCTTCAGGGACCCAAGATCGATGGGCACTGGTGGTGGATATTGGAAGGGATGGTCAAGCATGTTGATGTCTGGGCTAAAGAGCTAGGTCTTAGTAATGTTACCCAGACCATACTTCAGAGAAAGCATCATAAGAGACGGGTTGTACAGAAACCTTTAGTAGAGTCCATTGTGAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

95

Amino Acids

10.83

Weight (kDa)

9.16

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019333)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0340371
rosa_roxburghii Rroxscaffold_4G00311230
rosa_samantha Rh1AG178400 Rh1BG145700 Rh1BG146800 Rh1CG165500 Rh1DG177700 Rh6BG086600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AcuI CTGAAG 2 cut(s) 95, 206
AfaI GTAC 2 cut(s) 91, 252
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 235
AoxI GGCC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 135
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 101
BccI CCATC 2 cut(s) 122, 151
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 2 cut(s) 23, 191
BglII AGATCT 1 cut(s) 19
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 3 cut(s) 79, 117, 118
BmsI GCATC 1 cut(s) 241
BpiI GAAGAC 1 cut(s) 101
Bsa29I ATCGAT 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 81, 140
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 81, 140
BseSI GKGCMC 1 cut(s) 135
BshFI GGCC 1 cut(s) 29
BshNI GGYRCC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 126
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 235
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 16, 135
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 3 cut(s) 19, 49, 123
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 82
BspDI ATCGAT 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 79, 117, 118
BspT107I GGYRCC 1 cut(s) 77
BsrGI TGTACA 1 cut(s) 250
BsrI ACTGG 2 cut(s) 81, 140
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 19, 49, 123
BssT1I CCWWGG 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 69, 197
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 3 cut(s) 22, 52, 126
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 3 cut(s) 19, 49, 123
BstNSI RCATGY 1 cut(s) 170
BstSLI GKGCMC 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 126
BtsCI GGATG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 126
Csp6I GTAC 2 cut(s) 90, 251
CviAII CATG 1 cut(s) 167
CviJI RGCY 4 cut(s) 14, 29, 182, 190
CviKI_1 RGCY 4 cut(s) 14, 29, 182, 190
CviQI GTAC 2 cut(s) 90, 251
DdeI CTNAG 2 cut(s) 69, 197
DpnI GATC 3 cut(s) 21, 51, 125
DpnII GATC 3 cut(s) 19, 49, 123
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 95, 206
EcoO109I RGGNCCY 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 1 cut(s) 170
FaiI YATR 4 cut(s) 39, 168, 218, 237
FaqI GGGAC 1 cut(s) 129
FatI CATG 1 cut(s) 166
FokI GGATG 1 cut(s) 169
FriOI GRGCYC 1 cut(s) 16
FspBI CTAG 2 cut(s) 23, 191
HaeIII GGCC 1 cut(s) 29
Hin1II CATG 1 cut(s) 170
HinfI GANTC 2 cut(s) 101, 269
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 225
HpyAV CCTTC 1 cut(s) 146
HpyF3I CTNAG 2 cut(s) 69, 197
Hsp92II CATG 1 cut(s) 170
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 3 cut(s) 19, 49, 123
LpnPI CCDG 7 cut(s) 30, 56, 94, 98, 121, 163, 224
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 23, 191
MaeIII GTNAC 1 cut(s) 205
MalI GATC 3 cut(s) 21, 51, 125
MboI GATC 3 cut(s) 19, 49, 123
MboII GAAGA 1 cut(s) 101
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 2 cut(s) 16, 135
MluCI AATT 1 cut(s) 283
MlyI GAGTC 1 cut(s) 278
MseI TTAA 1 cut(s) 286
MslI CAYNNNNRTG 1 cut(s) 171
NdeII GATC 3 cut(s) 19, 49, 123
NlaIII CATG 1 cut(s) 170
NlaIV GGNNCC 3 cut(s) 79, 117, 118
NspI RCATGY 1 cut(s) 170
PfeI GAWTC 1 cut(s) 101
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 79, 117, 118
PspPI GGNCC 1 cut(s) 116
PspPPI RGGWCCY 1 cut(s) 116
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 91, 252
RsaNI GTAC 2 cut(s) 90, 251
RseI CAYNNNNRTG 1 cut(s) 171
SaqAI TTAA 1 cut(s) 286
Sau3AI GATC 3 cut(s) 19, 49, 123
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 1 cut(s) 278
SduI GDGCHC 2 cut(s) 16, 135
SetI ASST 6 cut(s) 48, 75, 95, 192, 196, 262
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 171
Sse9I AATT 1 cut(s) 283
SspMI CTAG 2 cut(s) 23, 191
StyI CCWWGG 1 cut(s) 30
TaqI TCGA 1 cut(s) 126
TasI AATT 1 cut(s) 283
TatI WGTACW 1 cut(s) 250
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 286
Tru9I TTAA 1 cut(s) 286
TscAI CASTG 1 cut(s) 140
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 116
XceI RCATGY 1 cut(s) 170
XspI CTAG 2 cut(s) 23, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.