Rh6BG086600

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0019333
Physical Location & Seq
Reverse (-)
13522577 .. 13539065
16489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG086600.1

Sequence Viewer

Length: 318 bp
ATGCTGATTAGAGCCCCAGATCTTGTGGCCTTGGAATATGCTGACCTTGATCTCACCCACAATGTTTCTCAGGTGCCAGTAGAAGTACCTTATTGGAATCAAGTCTTCAGGGACCCAAGATCGATGGGCACTGGTGGTGGATATTGGAAGGGTGCCCTGACCATTCTTCAGGAGAAAGCGTCATACGAGATGGGTTGTACAAAAGCCTTTAGTAGAGTCCAATGTGAACAATTAAGTTCGAAGCATCCAGAGGGCGTTTTGGAGCATACCTTTGCTAATAGAGTTGTTTGTGTGTGTCACAAGGACGTTGACCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

105

Amino Acids

11.78

Weight (kDa)

5.73

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019333)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0340371
rosa_roxburghii Rroxscaffold_4G00311230
rosa_samantha Rh1AG178400 Rh1BG145700 Rh1BG146800 Rh1CG165500 Rh1DG177700 Rh6BG086600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 73, 152
AcuI CTGAAG 2 cut(s) 91, 152
AfaI GTAC 2 cut(s) 87, 199
AoxI GGCC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 2 cut(s) 131, 157
BanI GGYRCC 2 cut(s) 73, 152
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 97
BccI CCATC 2 cut(s) 118, 184
BglII AGATCT 1 cut(s) 19
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 4 cut(s) 75, 113, 114, 154
BmsI GCATC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 97
Bpu14I TTCGAA 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bse1I ACTGG 2 cut(s) 77, 136
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 77, 136
BseSI GKGCMC 2 cut(s) 131, 157
BshFI GGCC 1 cut(s) 29
BshNI GGYRCC 2 cut(s) 73, 152
BshVI ATCGAT 1 cut(s) 122
BslFI GGGAC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 1 cut(s) 29
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 3 cut(s) 16, 131, 157
Bsp1407I TGTACA 1 cut(s) 197
Bsp143I GATC 3 cut(s) 19, 49, 119
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 82
BspDI ATCGAT 1 cut(s) 122
BspLI GGNNCC 4 cut(s) 75, 113, 114, 154
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 73, 152
BsrGI TGTACA 1 cut(s) 197
BsrI ACTGG 2 cut(s) 77, 136
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 19, 49, 119
BssT1I CCWWGG 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 3 cut(s) 22, 52, 122
BstMBI GATC 3 cut(s) 19, 49, 119
BstSLI GKGCMC 2 cut(s) 131, 157
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 122
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 1 cut(s) 168
Csp6I GTAC 2 cut(s) 86, 198
CviJI RGCY 3 cut(s) 14, 29, 206
CviKI_1 RGCY 3 cut(s) 14, 29, 206
CviQI GTAC 2 cut(s) 86, 198
DdeI CTNAG 1 cut(s) 69
DpnI GATC 3 cut(s) 21, 51, 121
DpnII GATC 3 cut(s) 19, 49, 119
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 2 cut(s) 91, 152
EcoO109I RGGNCCY 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 30
FaiI YATR 4 cut(s) 39, 184, 267, 316
FaqI GGGAC 1 cut(s) 125
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 1 cut(s) 16
HaeIII GGCC 1 cut(s) 29
HgaI GACGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 2 cut(s) 97, 216
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 227, 310
Hpy188III TCNNGA 2 cut(s) 170, 248
Hpy8I GTNNAC 2 cut(s) 227, 310
HpyAV CCTTC 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 306
HpyF3I CTNAG 1 cut(s) 69
HpySE526I ACGT 1 cut(s) 306
KflI GGGWCCC 1 cut(s) 112
Kzo9I GATC 3 cut(s) 19, 49, 119
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 8 cut(s) 30, 56, 90, 94, 117, 155, 170, 261
LweI GCATC 1 cut(s) 253
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 296
MalI GATC 3 cut(s) 21, 51, 121
MboI GATC 3 cut(s) 19, 49, 119
MboII GAAGA 2 cut(s) 97, 158
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 3 cut(s) 16, 131, 157
MluCI AATT 1 cut(s) 230
MlyI GAGTC 1 cut(s) 225
MnlI CCTC 1 cut(s) 244
MseI TTAA 1 cut(s) 233
NdeII GATC 3 cut(s) 19, 49, 119
NlaIV GGNNCC 4 cut(s) 75, 113, 114, 154
NmuCI GTSAC 1 cut(s) 296
NspV TTCGAA 1 cut(s) 239
PfeI GAWTC 1 cut(s) 97
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
PpuMI RGGWCCY 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 112
PspN4I GGNNCC 4 cut(s) 75, 113, 114, 154
PspPI GGNCC 1 cut(s) 112
PspPPI RGGWCCY 1 cut(s) 112
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 87, 199
RsaNI GTAC 2 cut(s) 86, 198
SaqAI TTAA 1 cut(s) 233
Sau3AI GATC 3 cut(s) 19, 49, 119
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 225
SduI GDGCHC 3 cut(s) 16, 131, 157
SetI ASST 5 cut(s) 48, 75, 91, 272, 309
SfaNI GCATC 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 239
SinI GGWCC 1 cut(s) 112
Sse9I AATT 1 cut(s) 230
StyI CCWWGG 1 cut(s) 30
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 122, 239
TasI AATT 1 cut(s) 230
TatI WGTACW 1 cut(s) 197
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 296
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.