RchiOBHm_Chr1g0341991

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0024727
Physical Location & Seq
Reverse (-)
33861171 .. 33862375
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 762 bp
ATGTCGACCTTGGCCAATCCCAAAGCTCACCACCATGAATTTGTTATCCAAGCAACACCAGTGAAGAGGCTGTGCAAAATCCACAACAGCATCACTGTGAATGGACAGTACCCTGGACCAACCTTGGTAGTAAACAATGGCGACACTCTGGTTGTCAAAGTTACCAACAAAGCTCGATACAAAGTCACAATTCACTGGCATGGTATTCGGCAAATGAGAACTGGATGGGCGGATGGGCCGGAATTTGTAACTCAATGCCCGATTAGGCCAGGAGGGAGTTACACCTACCGGTTCACAGTCCAGGGCCAAGAAGCACTTATTATTCATCCAAAACAAGGAGACTCGTATCCATTCCCTACATCAAATCGTCAAGCAACACTTCTTCTCGGTGAGTGGTGGGACGCTAACCCTATTGACGTGGTGAGGAGAGCAACTCTCACAGGAGCAGCTCCGAATGTTTCTGATGCATACACCATCAACGGTCAACCTGGTGATCTTTACAATTGCTCCAGCAAAGGGACTGTCGTAGTTCCCATAAACTCCGGTAAGACCAACCTTTTGAGAGTGATAAACGCCGCCCTCAACCAACCTCTTTTCTTCTCCGTCGCCAACCACAAGCTGACTGTGGTCGGTGCTGATGCCTCCTACACTAATGCTAGGACCCGGCCAGACCACCGATGTTTTAATCACCGGGGACCAGCCACCATCTCGATACTACTTGGCGGCACGTGCATATTTCAGTGCACAAAACGCGCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

253

Amino Acids

27.77

Weight (kDa)

9.75

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 16 - 107 7.6e-33 Multicopper oxidase
Cu-oxidase PF00394 125 - 219 6.9e-23 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024727)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341991
rosa_multiflora Rmu_co8407345.1_g000001
rosa_samantha Rh1CG158400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 753
AciI CCGC 3 cut(s) 230, 576, 723
AcoI YGGCCR 2 cut(s) 12, 665
AcsI RAATTY 2 cut(s) 38, 242
AcvI CACGTG 1 cut(s) 729
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 335, 516
AgeI ACCGGT 1 cut(s) 288
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 4 cut(s) 112, 268, 300, 487
AluBI AGCT 4 cut(s) 26, 173, 449, 619
AluI AGCT 4 cut(s) 26, 173, 449, 619
Alw21I GWGCWC 1 cut(s) 746
Alw26I GTCTC 1 cut(s) 333
Alw44I GTGCAC 1 cut(s) 742
AoxI GGCC 5 cut(s) 12, 236, 266, 304, 665
ApaLI GTGCAC 1 cut(s) 742
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 38, 242
ArsI GACNNNNNNTTYG 4 cut(s) 507, 539, 541, 573
AsiGI ACCGGT 1 cut(s) 288
AspLEI GCGC 1 cut(s) 755
AspS9I GGNCC 5 cut(s) 116, 236, 304, 660, 695
AsuC2I CCSGG 2 cut(s) 664, 692
AsuHPI GGTGA 5 cut(s) 20, 401, 433, 503, 680
AvaII GGWCC 3 cut(s) 116, 660, 695
BaeGI GKGCMC 1 cut(s) 746
BalI TGGCCA 1 cut(s) 14
BbrPI CACGTG 1 cut(s) 729
Bbv12I GWGCWC 1 cut(s) 746
BbvI GCAGC 1 cut(s) 458
BccI CCATC 4 cut(s) 219, 227, 482, 713
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 4 cut(s) 114, 270, 302, 489
BciVI GTATCC 1 cut(s) 357
BcnI CCSGG 2 cut(s) 664, 692
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 1 cut(s) 657
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 3 cut(s) 447, 576, 724
BlsI GCNGC 3 cut(s) 448, 577, 725
Bme1390I CCNGG 6 cut(s) 114, 270, 302, 489, 664, 692
Bme18I GGWCC 3 cut(s) 116, 660, 695
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 5 cut(s) 116, 236, 304, 660, 695
BmiI GGNNCC 2 cut(s) 662, 696
BmrFI CCNGG 6 cut(s) 114, 270, 302, 489, 664, 692
BmsI GCATC 3 cut(s) 99, 454, 628
BoxI GACNNNNGTC 1 cut(s) 626
BplI GAGNNNNNCTC 4 cut(s) 418, 450, 420, 452
BpmI CTGGAG 1 cut(s) 493
BpuMI CCSGG 2 cut(s) 664, 692
BsaAI YACGTR 1 cut(s) 729
BsaJI CCNNGG 5 cut(s) 9, 112, 123, 301, 691
BsaWI WCCGGW 2 cut(s) 288, 542
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 516
Bse118I RCCGGY 1 cut(s) 288
Bse1I ACTGG 3 cut(s) 59, 200, 226
BseBI CCWGG 4 cut(s) 114, 270, 302, 489
BseDI CCNNGG 5 cut(s) 9, 112, 123, 301, 691
BseGI GGATG 3 cut(s) 230, 238, 325
BseLI CCNNNNNNNGG 2 cut(s) 335, 516
BseNI ACTGG 3 cut(s) 59, 200, 226
BseRI GAGGAG 1 cut(s) 439
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 5 cut(s) 14, 238, 268, 306, 667
BshTI ACCGGT 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 746
BsiSI CCGG 5 cut(s) 239, 289, 543, 664, 691
BslFI GGGAC 3 cut(s) 413, 532, 708
BslI CCNNNNNNNGG 2 cut(s) 335, 516
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 3 cut(s) 413, 532, 708
BsnI GGCC 5 cut(s) 14, 238, 268, 306, 667
Bsp1286I GDGCHC 1 cut(s) 746
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 3 cut(s) 230, 576, 723
BspANI GGCC 5 cut(s) 14, 238, 268, 306, 667
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 2 cut(s) 662, 696
BsrFI RCCGGY 1 cut(s) 288
BsrI ACTGG 3 cut(s) 59, 200, 226
BssAI RCCGGY 1 cut(s) 288
BssECI CCNNGG 5 cut(s) 9, 112, 123, 301, 691
BssMI GATC 1 cut(s) 493
BssT1I CCWWGG 2 cut(s) 9, 123
Bst2UI CCWGG 4 cut(s) 114, 270, 302, 489
Bst4CI ACNGT 6 cut(s) 97, 108, 298, 482, 523, 625
Bst6I CTCTTC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 729
BstF5I GGATG 3 cut(s) 230, 238, 325
BstFNI CGCG 1 cut(s) 753
BstHHI GCGC 1 cut(s) 755
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 1 cut(s) 493
BstMWI GCNNNNNNNGC 2 cut(s) 729, 750
BstNI CCWGG 4 cut(s) 114, 270, 302, 489
BstPAI GACNNNNGTC 1 cut(s) 626
BstSCI CCNGG 6 cut(s) 112, 268, 300, 487, 662, 690
BstSLI GKGCMC 1 cut(s) 746
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 458
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 5 cut(s) 14, 238, 268, 306, 667
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 3 cut(s) 230, 238, 325
BtsIMutI CAGTG 4 cut(s) 66, 93, 193, 746
CfoI GCGC 1 cut(s) 755
Cfr10I RCCGGY 1 cut(s) 288
Cfr13I GGNCC 5 cut(s) 116, 236, 304, 660, 695
CseI GACGC 1 cut(s) 410
CsiI ACCWGGT 1 cut(s) 487
Csp6I GTAC 1 cut(s) 109
CspAI ACCGGT 1 cut(s) 288
CviAII CATG 2 cut(s) 35, 200
CviQI GTAC 1 cut(s) 109
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
EaeI YGGCCR 2 cut(s) 12, 665
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 9, 123
Eco47I GGWCC 3 cut(s) 116, 660, 695
Eco72I CACGTG 1 cut(s) 729
EcoO109I RGGNCCY 1 cut(s) 660
EcoRII CCWGG 4 cut(s) 112, 268, 300, 487
EcoT14I CCWWGG 2 cut(s) 9, 123
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 2 cut(s) 9, 123
FaeI CATG 2 cut(s) 38, 203
FaiI YATR 5 cut(s) 36, 201, 469, 536, 734
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 363, 395
FaqI GGGAC 3 cut(s) 413, 532, 708
FatI CATG 2 cut(s) 34, 199
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 447, 576, 724
FokI GGATG 3 cut(s) 237, 245, 312
Fsp4HI GCNGC 3 cut(s) 447, 576, 724
FspBI CTAG 1 cut(s) 657
GlaI GCGC 1 cut(s) 754
GluI GCNGC 3 cut(s) 447, 576, 724
GsuI CTGGAG 1 cut(s) 493
HaeIII GGCC 5 cut(s) 14, 238, 268, 306, 667
HapII CCGG 5 cut(s) 239, 289, 543, 664, 691
HgaI GACGC 1 cut(s) 410
HhaI GCGC 1 cut(s) 755
Hin1II CATG 2 cut(s) 38, 203
Hin6I GCGC 1 cut(s) 753
HinP1I GCGC 1 cut(s) 753
HincII GTYRAC 2 cut(s) 6, 485
HindII GTYRAC 2 cut(s) 6, 485
HinfI GANTC 1 cut(s) 341
HpaII CCGG 5 cut(s) 239, 289, 543, 664, 691
HphI GGTGA 5 cut(s) 20, 401, 433, 503, 680
Hpy166II GTNNAC 5 cut(s) 6, 133, 294, 485, 744
Hpy188I TCNGA 2 cut(s) 453, 463
Hpy188III TCNNGA 1 cut(s) 709
Hpy8I GTNNAC 5 cut(s) 6, 133, 294, 485, 744
Hpy99I CGWCG 1 cut(s) 608
HpyCH4III ACNGT 6 cut(s) 97, 108, 298, 482, 523, 625
HpyCH4IV ACGT 2 cut(s) 417, 728
HpyCH4V TGCA 4 cut(s) 75, 467, 732, 744
HpyF10VI GCNNNNNNNGC 2 cut(s) 729, 750
HpySE526I ACGT 2 cut(s) 417, 728
Hsp92II CATG 2 cut(s) 38, 203
HspAI GCGC 1 cut(s) 753
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 3 cut(s) 443, 454, 512
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 3 cut(s) 99, 454, 628
MabI ACCWGGT 1 cut(s) 487
MaeI CTAG 1 cut(s) 657
MaeII ACGT 2 cut(s) 417, 728
MaeIII GTNAC 4 cut(s) 160, 184, 247, 278
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 3 cut(s) 76, 374, 589
MfeI CAATTG 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 746
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 4 cut(s) 38, 189, 242, 502
MluNI TGGCCA 1 cut(s) 14
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 6 cut(s) 60, 266, 417, 590, 600, 652
Mox20I TGGCCA 1 cut(s) 14
Mph1103I ATGCAT 1 cut(s) 469
MscI TGGCCA 1 cut(s) 14
MseI TTAA 1 cut(s) 684
MslI CAYNNNNRTG 3 cut(s) 33, 95, 198
Msp20I TGGCCA 1 cut(s) 14
MspI CCGG 5 cut(s) 239, 289, 543, 664, 691
MspR9I CCNGG 6 cut(s) 114, 270, 302, 489, 664, 692
MunI CAATTG 1 cut(s) 502
MvaI CCWGG 4 cut(s) 114, 270, 302, 489
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 2 cut(s) 729, 750
NciI CCSGG 2 cut(s) 664, 692
NdeII GATC 1 cut(s) 493
NlaIII CATG 2 cut(s) 38, 203
NlaIV GGNNCC 2 cut(s) 662, 696
NmuCI GTSAC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 469
PinAI ACCGGT 1 cut(s) 288
PkrI GCNGC 3 cut(s) 448, 577, 725
PleI GAGTC 1 cut(s) 335
PmaCI CACGTG 1 cut(s) 729
PmlI CACGTG 1 cut(s) 729
PpsI GAGTC 1 cut(s) 335
Ppu21I YACGTR 1 cut(s) 729
PpuMI RGGWCCY 1 cut(s) 660
PshAI GACNNNNGTC 1 cut(s) 626
Psp5II RGGWCCY 1 cut(s) 660
Psp6I CCWGG 4 cut(s) 112, 268, 300, 487
PspCI CACGTG 1 cut(s) 729
PspGI CCWGG 4 cut(s) 112, 268, 300, 487
PspN4I GGNNCC 2 cut(s) 662, 696
PspPI GGNCC 5 cut(s) 116, 236, 304, 660, 695
PspPPI RGGWCCY 1 cut(s) 660
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 3 cut(s) 33, 95, 198
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 684
SatI GCNGC 3 cut(s) 447, 576, 724
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 5 cut(s) 116, 236, 304, 660, 695
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 6 cut(s) 114, 270, 302, 489, 664, 692
SduI GDGCHC 1 cut(s) 746
SexAI ACCWGGT 1 cut(s) 487
SfaNI GCATC 3 cut(s) 99, 454, 628
SinI GGWCC 3 cut(s) 116, 660, 695
SmiMI CAYNNNNRTG 3 cut(s) 33, 95, 198
Sse9I AATT 4 cut(s) 38, 189, 242, 502
SsiI CCGC 3 cut(s) 230, 576, 723
SspMI CTAG 1 cut(s) 657
StyD4I CCNGG 6 cut(s) 112, 268, 300, 487, 662, 690
StyI CCWWGG 2 cut(s) 9, 123
TaaI ACNGT 6 cut(s) 97, 108, 298, 482, 523, 625
TaiI ACGT 2 cut(s) 420, 731
TaqI TCGA 3 cut(s) 5, 175, 710
TasI AATT 4 cut(s) 38, 189, 242, 502
TauI GCSGC 2 cut(s) 578, 726
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 4 cut(s) 66, 100, 200, 746
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 51, 314
TspGWI ACGGA 1 cut(s) 592
TspRI CASTG 4 cut(s) 66, 100, 200, 746
VneI GTGCAC 1 cut(s) 742
VpaK11BI GGWCC 3 cut(s) 116, 660, 695
XapI RAATTY 2 cut(s) 38, 242
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 657
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.