Rh1CG158400

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0024727
Physical Location & Seq
Forward (+)
35095040 .. 35095940
901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG158400.1

Sequence Viewer

Length: 612 bp
ATGTCGACCTTGGACAATCCCAAAGCTCACCACCATGAATTTGTTATCCAACCAGCACCAGTGAAGAGGCTGTGCAAAATCCACAACAGCATCACTGTGAATGGACAGTACCCTGGACCAACCTTGGTAGTAAACAATGGCGACACTCTGGTTGTCAAAGTTACCAACAAAGCTCGATACAACGTCACAATTCACTGGCATGGTATTCGGCAAATGAGAACTGGATGGGCGGATGGGCCCGAATTTGTAACTCAGTGCCCGATAATGCCAGGAGGGAGTTACACTTACCGGTTCACAGTCCAGGGCCAAGAAGGTACCCTGTGGTGGCACGCTCATAGCTCATGGCTTAGAGCCACTGTGTATGGAGCACTTATTATTCATCCAAAACAAGGAGACTCGTATCCATTCCCTAAACCAAACCATCAAGCAACACTTCTTCTCGGTGAGTGGTGGGACGCTAACCCTATTGACGTGGTGAGGATAGCAACTCGCACAGGAGCAGGTCCGAATGTTTCTGATGCATACACCATCAACGGTCAACCTGGTGATCTTTACAATTGCTCCAGCAAAGGTTTGGTTCTTAATCCCCCATTCAATATATCTCTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

203

Amino Acids

22.58

Weight (kDa)

9.17

Isoelectric Point (pI)

27.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 17 - 130 1.2e-44 Multicopper oxidase
Cu-oxidase PF00394 143 - 189 4.1e-06 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024727)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341991
rosa_multiflora Rmu_co8407345.1_g000001
rosa_samantha Rh1CG158400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 491
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 38, 242
AfaI GTAC 2 cut(s) 110, 316
AfiI CCNNNNNNNGG 2 cut(s) 324, 389
AgeI ACCGGT 1 cut(s) 288
AgsI TTSAA 1 cut(s) 595
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 4 cut(s) 112, 268, 300, 541
AluBI AGCT 3 cut(s) 26, 173, 339
AluI AGCT 3 cut(s) 26, 173, 339
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 1 cut(s) 387
AoxI GGCC 2 cut(s) 236, 304
ApaI GGGCCC 1 cut(s) 240
ApoI RAATTY 2 cut(s) 38, 242
AsiGI ACCGGT 1 cut(s) 288
Asp718I GGTACC 1 cut(s) 314
AspS9I GGNCC 5 cut(s) 116, 236, 237, 304, 503
AsuHPI GGTGA 4 cut(s) 20, 455, 487, 557
AvaII GGWCC 2 cut(s) 116, 503
BaeGI GKGCMC 2 cut(s) 240, 260
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 370
BccI CCATC 4 cut(s) 219, 227, 429, 536
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 4 cut(s) 114, 270, 302, 543
BciVI GTATCC 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 491
BfuI GTATCC 1 cut(s) 411
Bme1390I CCNGG 4 cut(s) 114, 270, 302, 543
Bme18I GGWCC 2 cut(s) 116, 503
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 5 cut(s) 116, 236, 237, 304, 503
BmiI GGNNCC 2 cut(s) 238, 316
BmrFI CCNGG 4 cut(s) 114, 270, 302, 543
BmsI GCATC 2 cut(s) 99, 508
BpmI CTGGAG 1 cut(s) 547
BsaJI CCNNGG 4 cut(s) 9, 112, 123, 301
BsaWI WCCGGW 1 cut(s) 288
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 389
Bse118I RCCGGY 1 cut(s) 288
Bse1I ACTGG 3 cut(s) 59, 200, 226
BseBI CCWGG 4 cut(s) 114, 270, 302, 543
BseDI CCNNGG 4 cut(s) 9, 112, 123, 301
BseGI GGATG 3 cut(s) 230, 238, 379
BseLI CCNNNNNNNGG 2 cut(s) 324, 389
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 3 cut(s) 59, 200, 226
BseSI GKGCMC 2 cut(s) 240, 260
BshFI GGCC 2 cut(s) 238, 306
BshNI GGYRCC 1 cut(s) 314
BshTI ACCGGT 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 289
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 2 cut(s) 324, 389
BsmAI GTCTC 1 cut(s) 387
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 2 cut(s) 238, 306
Bsp120I GGGCCC 1 cut(s) 236
Bsp1286I GDGCHC 3 cut(s) 240, 260, 370
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 1 cut(s) 230
BspANI GGCC 2 cut(s) 238, 306
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 238, 316
BspMI ACCTGC 1 cut(s) 491
BspT107I GGYRCC 1 cut(s) 314
BsrFI RCCGGY 1 cut(s) 288
BsrI ACTGG 3 cut(s) 59, 200, 226
BssAI RCCGGY 1 cut(s) 288
BssECI CCNNGG 4 cut(s) 9, 112, 123, 301
BssMI GATC 1 cut(s) 547
BssT1I CCWWGG 2 cut(s) 9, 123
Bst2UI CCWGG 4 cut(s) 114, 270, 302, 543
Bst4CI ACNGT 5 cut(s) 97, 108, 298, 358, 536
Bst6I CTCTTC 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 252, 347
BstF5I GGATG 3 cut(s) 230, 238, 379
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 1 cut(s) 547
BstNI CCWGG 4 cut(s) 114, 270, 302, 543
BstSCI CCNGG 4 cut(s) 112, 268, 300, 541
BstSLI GKGCMC 2 cut(s) 240, 260
BsuI GTATCC 1 cut(s) 411
BsuRI GGCC 2 cut(s) 238, 306
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 3 cut(s) 230, 238, 379
BtsIMutI CAGTG 5 cut(s) 66, 93, 193, 260, 354
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 288
Cfr13I GGNCC 5 cut(s) 116, 236, 237, 304, 503
CseI GACGC 1 cut(s) 464
CsiI ACCWGGT 1 cut(s) 541
Csp6I GTAC 2 cut(s) 109, 315
CspAI ACCGGT 1 cut(s) 288
CviAII CATG 3 cut(s) 35, 200, 342
CviJI RGCY 8 cut(s) 26, 70, 173, 238, 306, 339, 346, 353
CviKI_1 RGCY 8 cut(s) 26, 70, 173, 238, 306, 339, 346, 353
CviQI GTAC 2 cut(s) 109, 315
DdeI CTNAG 2 cut(s) 252, 347
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 9, 123
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 2 cut(s) 116, 503
EcoRII CCWGG 4 cut(s) 112, 268, 300, 541
EcoT14I CCWWGG 2 cut(s) 9, 123
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 2 cut(s) 9, 123
FaeI CATG 3 cut(s) 38, 203, 345
FaiI YATR 7 cut(s) 36, 201, 336, 343, 363, 523, 599
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 467
FatI CATG 3 cut(s) 34, 199, 341
FblI GTMKAC 1 cut(s) 5
FokI GGATG 3 cut(s) 237, 245, 366
FriOI GRGCYC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 547
HaeIII GGCC 2 cut(s) 238, 306
HapII CCGG 1 cut(s) 289
HgaI GACGC 1 cut(s) 464
Hin1II CATG 3 cut(s) 38, 203, 345
HincII GTYRAC 2 cut(s) 6, 539
HindII GTYRAC 2 cut(s) 6, 539
HinfI GANTC 1 cut(s) 395
HpaII CCGG 1 cut(s) 289
HphI GGTGA 4 cut(s) 20, 455, 487, 557
Hpy166II GTNNAC 4 cut(s) 6, 133, 294, 539
Hpy188I TCNGA 2 cut(s) 507, 517
Hpy8I GTNNAC 4 cut(s) 6, 133, 294, 539
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 5 cut(s) 97, 108, 298, 358, 536
HpyCH4IV ACGT 2 cut(s) 183, 471
HpyCH4V TGCA 2 cut(s) 75, 521
HpyF3I CTNAG 2 cut(s) 252, 347
HpySE526I ACGT 2 cut(s) 183, 471
Hsp92II CATG 3 cut(s) 38, 203, 345
KpnI GGTACC 1 cut(s) 318
Kzo9I GATC 1 cut(s) 547
LmnI GCTCC 3 cut(s) 365, 497, 566
LweI GCATC 2 cut(s) 99, 508
MabI ACCWGGT 1 cut(s) 541
MaeII ACGT 2 cut(s) 183, 471
MaeIII GTNAC 4 cut(s) 160, 184, 247, 278
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 2 cut(s) 76, 428
MfeI CAATTG 1 cut(s) 556
MhlI GDGCHC 3 cut(s) 240, 260, 370
MluCI AATT 4 cut(s) 38, 189, 242, 556
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 3 cut(s) 60, 266, 471
Mph1103I ATGCAT 1 cut(s) 523
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 3 cut(s) 33, 95, 198
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 4 cut(s) 114, 270, 302, 543
MunI CAATTG 1 cut(s) 556
MvaI CCWGG 4 cut(s) 114, 270, 302, 543
NdeII GATC 1 cut(s) 547
NlaIII CATG 3 cut(s) 38, 203, 345
NlaIV GGNNCC 2 cut(s) 238, 316
NmuCI GTSAC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 523
PinAI ACCGGT 1 cut(s) 288
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp6I CCWGG 4 cut(s) 112, 268, 300, 541
PspGI CCWGG 4 cut(s) 112, 268, 300, 541
PspN4I GGNNCC 2 cut(s) 238, 316
PspOMI GGGCCC 1 cut(s) 236
PspPI GGNCC 5 cut(s) 116, 236, 237, 304, 503
RsaI GTAC 2 cut(s) 110, 316
RsaNI GTAC 2 cut(s) 109, 315
RseI CAYNNNNRTG 3 cut(s) 33, 95, 198
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 582
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 5 cut(s) 116, 236, 237, 304, 503
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 4 cut(s) 114, 270, 302, 543
SduI GDGCHC 3 cut(s) 240, 260, 370
SexAI ACCWGGT 1 cut(s) 541
SfaNI GCATC 2 cut(s) 99, 508
SinI GGWCC 2 cut(s) 116, 503
SmiMI CAYNNNNRTG 3 cut(s) 33, 95, 198
Sse9I AATT 4 cut(s) 38, 189, 242, 556
SsiI CCGC 1 cut(s) 230
StyD4I CCNGG 4 cut(s) 112, 268, 300, 541
StyI CCWWGG 2 cut(s) 9, 123
TaaI ACNGT 5 cut(s) 97, 108, 298, 358, 536
TaiI ACGT 2 cut(s) 186, 474
TaqI TCGA 2 cut(s) 5, 175
TasI AATT 4 cut(s) 38, 189, 242, 556
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 5 cut(s) 66, 100, 200, 260, 361
TseFI GTSAC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 51, 368
TspRI CASTG 5 cut(s) 66, 100, 200, 260, 361
VpaK11BI GGWCC 2 cut(s) 116, 503
XapI RAATTY 2 cut(s) 38, 242
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmiI GTMKAC 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.