Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr1g0348111 |
| rosa_samantha | Rh1BG175900 Rh1DG205700 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 79 |
| AcsI | RAATTY | 1 cut(s) | 52 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 78, 164 |
| AflIII | ACRYGT | 1 cut(s) | 17 |
| AgsI | TTSAA | 1 cut(s) | 202 |
| AluBI | AGCT | 2 cut(s) | 208, 261 |
| AluI | AGCT | 2 cut(s) | 208, 261 |
| AoxI | GGCC | 1 cut(s) | 62 |
| ApoI | RAATTY | 1 cut(s) | 52 |
| AsuHPI | GGTGA | 1 cut(s) | 139 |
| BbsI | GAAGAC | 1 cut(s) | 47 |
| BbvCI | CCTCAGC | 1 cut(s) | 93 |
| BclI | TGATCA | 1 cut(s) | 192 |
| BfaI | CTAG | 2 cut(s) | 48, 135 |
| BpiI | GAAGAC | 1 cut(s) | 47 |
| Bpu10I | CCTNAGC | 1 cut(s) | 93 |
| BsaBI | GATNNNNATC | 1 cut(s) | 191 |
| BsaJI | CCNNGG | 1 cut(s) | 59 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 78, 164 |
| Bse3DI | GCAATG | 1 cut(s) | 40 |
| Bse8I | GATNNNNATC | 1 cut(s) | 191 |
| BseDI | CCNNGG | 1 cut(s) | 59 |
| BseJI | GATNNNNATC | 1 cut(s) | 191 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 78, 164 |
| BseMI | GCAATG | 1 cut(s) | 40 |
| BseMII | CTCAG | 2 cut(s) | 84, 174 |
| BshFI | GGCC | 1 cut(s) | 64 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 78, 164 |
| BsnI | GGCC | 1 cut(s) | 64 |
| Bsp143I | GATC | 3 cut(s) | 160, 186, 192 |
| BspACI | CCGC | 1 cut(s) | 79 |
| BspANI | GGCC | 1 cut(s) | 64 |
| BspCNI | CTCAG | 2 cut(s) | 85, 175 |
| BsrDI | GCAATG | 1 cut(s) | 40 |
| BssECI | CCNNGG | 1 cut(s) | 59 |
| BssMI | GATC | 3 cut(s) | 160, 186, 192 |
| BstC8I | GCNNGC | 1 cut(s) | 81 |
| BstDEI | CTNAG | 2 cut(s) | 93, 183 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 162 |
| BstKTI | GATC | 3 cut(s) | 163, 189, 195 |
| BstMBI | GATC | 3 cut(s) | 160, 186, 192 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 89 |
| BstNSI | RCATGY | 1 cut(s) | 21 |
| BstV2I | GAAGAC | 1 cut(s) | 47 |
| BsuRI | GGCC | 1 cut(s) | 64 |
| Cac8I | GCNNGC | 1 cut(s) | 81 |
| CseI | GACGC | 1 cut(s) | 93 |
| CviAII | CATG | 1 cut(s) | 18 |
| CviJI | RGCY | 5 cut(s) | 64, 92, 208, 233, 261 |
| CviKI_1 | RGCY | 5 cut(s) | 64, 92, 208, 233, 261 |
| DdeI | CTNAG | 2 cut(s) | 93, 183 |
| DpnI | GATC | 3 cut(s) | 162, 188, 194 |
| DpnII | GATC | 3 cut(s) | 160, 186, 192 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 162 |
| EcoT22I | ATGCAT | 1 cut(s) | 27 |
| FaeI | CATG | 1 cut(s) | 21 |
| FaiI | YATR | 7 cut(s) | 19, 23, 27, 123, 155, 221, 242 |
| FatI | CATG | 1 cut(s) | 17 |
| FauI | CCCGC | 1 cut(s) | 72 |
| FbaI | TGATCA | 1 cut(s) | 192 |
| FspBI | CTAG | 2 cut(s) | 48, 135 |
| HaeIII | GGCC | 1 cut(s) | 64 |
| HgaI | GACGC | 1 cut(s) | 93 |
| Hin1II | CATG | 1 cut(s) | 21 |
| HinfI | GANTC | 1 cut(s) | 4 |
| HphI | GGTGA | 1 cut(s) | 139 |
| Hpy166II | GTNNAC | 1 cut(s) | 173 |
| Hpy188III | TCNNGA | 2 cut(s) | 48, 190 |
| Hpy8I | GTNNAC | 1 cut(s) | 173 |
| Hpy99I | CGWCG | 1 cut(s) | 109 |
| HpyCH4V | TGCA | 2 cut(s) | 25, 33 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 89 |
| HpyF3I | CTNAG | 2 cut(s) | 93, 183 |
| Hsp92II | CATG | 1 cut(s) | 21 |
| Ksp22I | TGATCA | 1 cut(s) | 192 |
| Kzo9I | GATC | 3 cut(s) | 160, 186, 192 |
| LpnPI | CCDG | 2 cut(s) | 78, 171 |
| MaeI | CTAG | 2 cut(s) | 48, 135 |
| MalI | GATC | 3 cut(s) | 162, 188, 194 |
| MboI | GATC | 3 cut(s) | 160, 186, 192 |
| MboII | GAAGA | 2 cut(s) | 52, 214 |
| MluCI | AATT | 2 cut(s) | 52, 99 |
| MmeI | TCCRAC | 1 cut(s) | 96 |
| MnlI | CCTC | 3 cut(s) | 54, 88, 158 |
| Mph1103I | ATGCAT | 1 cut(s) | 27 |
| MseI | TTAA | 2 cut(s) | 228, 236 |
| MslI | CAYNNNNRTG | 1 cut(s) | 20 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 89 |
| NdeII | GATC | 3 cut(s) | 160, 186, 192 |
| NlaIII | CATG | 1 cut(s) | 21 |
| NmeAIII | GCCGAG | 1 cut(s) | 84 |
| NsiI | ATGCAT | 1 cut(s) | 27 |
| NspI | RCATGY | 1 cut(s) | 21 |
| PciI | ACATGT | 1 cut(s) | 17 |
| PfeI | GAWTC | 1 cut(s) | 4 |
| PscI | ACATGT | 1 cut(s) | 17 |
| RseI | CAYNNNNRTG | 1 cut(s) | 20 |
| SaqAI | TTAA | 2 cut(s) | 228, 236 |
| Sau3AI | GATC | 3 cut(s) | 160, 186, 192 |
| SetI | ASST | 4 cut(s) | 99, 210, 217, 263 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 20 |
| Sse9I | AATT | 2 cut(s) | 52, 99 |
| SsiI | CCGC | 1 cut(s) | 79 |
| SspMI | CTAG | 2 cut(s) | 48, 135 |
| TaqI | TCGA | 2 cut(s) | 163, 254 |
| TasI | AATT | 2 cut(s) | 52, 99 |
| TfiI | GAWTC | 1 cut(s) | 4 |
| Tru1I | TTAA | 2 cut(s) | 228, 236 |
| Tru9I | TTAA | 2 cut(s) | 228, 236 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 162 |
| XapI | RAATTY | 1 cut(s) | 52 |
| XbaI | TCTAGA | 1 cut(s) | 47 |
| XceI | RCATGY | 1 cut(s) | 21 |
| XspI | CTAG | 2 cut(s) | 48, 135 |
| Zsp2I | ATGCAT | 1 cut(s) | 27 |