Rh1BG175900

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0024784
Physical Location & Seq
Reverse (-)
28011433 .. 28011905
473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG175900.1

Sequence Viewer

Length: 360 bp
ATGATTCTCGGCGACATGTGTACGCGCAGTTGCAGGTTTTGTAGTGTGAAGACTTCTAGGACTTCGCCGCGGTCGGACCCCAATAAGCCGGCGAATGTGGCTGAGGCGATTGCGTCGTGGGGGTTGGATTATGTGGTGATTACTAGTGTGGACCGGGATGATATGCCTGATCAAGGGAGTGGACATTTTGCTGAGATCGTGATCAAGTTGAAGAATCTCAAACCCAATATGCTCATCAAGGCTTTAAGGGTGTGGGATGATTACTGTGGGCTGCTCTTTCATGGTCATGGGGGCATTAGCAGCGGCGACCATGACGCCTGGATCGGGGCTTTGAAGGGATGGACAGACCCTTTGAGATAG

Protein Analysis

119

Amino Acids

13.12

Weight (kDa)

6.88

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 1 - 71 3.3e-08 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024784)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0348111
rosa_samantha Rh1BG175900 Rh1DG205700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 24
AccII CGCG 2 cut(s) 25, 70
AciI CCGC 3 cut(s) 68, 70, 303
AclWI GGATC 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 173, 324
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 2 cut(s) 211, 334
AhlI ACTAGT 1 cut(s) 143
AjnI CCWGG 1 cut(s) 317
AlwI GGATC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 271, 300
ArsI GACNNNNNNTTYG 1 cut(s) 334
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 76, 151
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 2 cut(s) 76, 151
BbsI GAAGAC 1 cut(s) 56
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 258, 312
BccI CCATC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 319
BclI TGATCA 2 cut(s) 169, 201
BcnI CCSGG 1 cut(s) 155
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 2 cut(s) 57, 144
BfuAI ACCTGC 1 cut(s) 24
BisI GCNGC 4 cut(s) 68, 272, 301, 304
BlsI GCNGC 4 cut(s) 69, 273, 302, 305
Bme1390I CCNGG 2 cut(s) 155, 319
Bme18I GGWCC 2 cut(s) 76, 151
BmgT120I GGNCC 2 cut(s) 76, 151
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 2 cut(s) 155, 319
BpiI GAAGAC 1 cut(s) 56
Bpu10I CCTNAGC 1 cut(s) 102
BpuMI CCSGG 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 68
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 324
Bse118I RCCGGY 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 3 cut(s) 163, 262, 344
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 2 cut(s) 173, 324
BseMII CTCAG 2 cut(s) 93, 183
BseXI GCAGC 2 cut(s) 258, 312
Bsh1236I CGCG 2 cut(s) 25, 70
Bsh1285I CGRYCG 1 cut(s) 74
BsiEI CGRYCG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 89, 154
BslI CCNNNNNNNGG 2 cut(s) 173, 324
Bsp143I GATC 4 cut(s) 169, 195, 201, 321
BspACI CCGC 3 cut(s) 68, 70, 303
BspCNI CTCAG 2 cut(s) 94, 184
BspFNI CGCG 2 cut(s) 25, 70
BspLI GGNNCC 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 4 cut(s) 169, 195, 201, 321
BssNI GRCGYC 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 315
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 2 cut(s) 102, 192
BstDSI CCRYGG 1 cut(s) 68
BstENI CCTNNNNNAGG 1 cut(s) 171
BstF5I GGATG 3 cut(s) 163, 262, 344
BstFNI CGCG 2 cut(s) 25, 70
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 4 cut(s) 172, 198, 204, 324
BstMBI GATC 4 cut(s) 169, 195, 201, 321
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 2 cut(s) 98, 300
BstNI CCWGG 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 2 cut(s) 153, 317
BstUI CGCG 2 cut(s) 25, 70
BstV1I GCAGC 2 cut(s) 258, 312
BstV2I GAAGAC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 3 cut(s) 163, 262, 344
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 90
CfoI GCGC 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 76, 151
Cfr42I CCGCGG 1 cut(s) 71
CseI GACGC 2 cut(s) 102, 323
Csp6I GTAC 1 cut(s) 21
CviAII CATG 4 cut(s) 16, 281, 287, 311
CviJI RGCY 5 cut(s) 88, 101, 242, 271, 329
CviKI_1 RGCY 5 cut(s) 88, 101, 242, 271, 329
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 2 cut(s) 102, 192
DpnI GATC 4 cut(s) 171, 197, 203, 323
DpnII GATC 4 cut(s) 169, 195, 201, 321
Eco47I GGWCC 2 cut(s) 76, 151
EcoNI CCTNNNNNAGG 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 317
FaeI CATG 4 cut(s) 19, 284, 290, 314
FaiI YATR 7 cut(s) 17, 132, 164, 230, 282, 288, 312
FatI CATG 4 cut(s) 15, 280, 286, 310
FbaI TGATCA 2 cut(s) 169, 201
Fnu4HI GCNGC 4 cut(s) 68, 272, 301, 304
FokI GGATG 3 cut(s) 170, 269, 351
Fsp4HI GCNGC 4 cut(s) 68, 272, 301, 304
FspBI CTAG 2 cut(s) 57, 144
GlaI GCGC 1 cut(s) 26
GluI GCNGC 4 cut(s) 68, 272, 301, 304
HapII CCGG 2 cut(s) 89, 154
HgaI GACGC 2 cut(s) 102, 323
HhaI GCGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 4 cut(s) 19, 284, 290, 314
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HinfI GANTC 2 cut(s) 4, 214
HpaII CCGG 2 cut(s) 89, 154
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 3 cut(s) 21, 151, 182
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 1 cut(s) 199
Hpy8I GTNNAC 3 cut(s) 21, 151, 182
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 300
HpyF3I CTNAG 2 cut(s) 102, 192
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 4 cut(s) 19, 284, 290, 314
HspAI GCGC 1 cut(s) 25
KroI GCCGGC 1 cut(s) 88
KroNI GCCGGC 1 cut(s) 90
Ksp22I TGATCA 2 cut(s) 169, 201
KspI CCGCGG 1 cut(s) 71
Kzo9I GATC 4 cut(s) 169, 195, 201, 321
LpnPI CCDG 6 cut(s) 19, 102, 167, 180, 304, 331
Lsp1109I GCAGC 2 cut(s) 258, 312
MaeI CTAG 2 cut(s) 57, 144
MalI GATC 4 cut(s) 171, 197, 203, 323
MboI GATC 4 cut(s) 169, 195, 201, 321
MboII GAAGA 2 cut(s) 61, 223
MmeI TCCRAC 2 cut(s) 54, 105
MnlI CCTC 1 cut(s) 97
MroNI GCCGGC 1 cut(s) 88
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 70, 303
MspI CCGG 2 cut(s) 89, 154
MspR9I CCNGG 2 cut(s) 155, 319
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 2 cut(s) 25, 70
MwoI GCNNNNNNNGC 2 cut(s) 98, 300
NaeI GCCGGC 1 cut(s) 90
NciI CCSGG 1 cut(s) 155
NdeII GATC 4 cut(s) 169, 195, 201, 321
NgoMIV GCCGGC 1 cut(s) 88
NlaIII CATG 4 cut(s) 19, 284, 290, 314
NlaIV GGNNCC 1 cut(s) 78
NspI RCATGY 1 cut(s) 19
PciI ACATGT 1 cut(s) 15
PdiI GCCGGC 1 cut(s) 90
PfeI GAWTC 2 cut(s) 4, 214
PkrI GCNGC 4 cut(s) 69, 273, 302, 305
PscI ACATGT 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 2 cut(s) 76, 151
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 285
SacII CCGCGG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 4 cut(s) 68, 272, 301, 304
Sau3AI GATC 4 cut(s) 169, 195, 201, 321
Sau96I GGNCC 2 cut(s) 76, 151
ScrFI CCNGG 2 cut(s) 155, 319
SetI ASST 1 cut(s) 38
Sfr303I CCGCGG 1 cut(s) 71
SgrBI CCGCGG 1 cut(s) 71
SinI GGWCC 2 cut(s) 76, 151
SmiMI CAYNNNNRTG 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 143
SsiI CCGC 3 cut(s) 68, 70, 303
SspMI CTAG 2 cut(s) 57, 144
StyD4I CCNGG 2 cut(s) 153, 317
TaaI ACNGT 1 cut(s) 266
TauI GCSGC 2 cut(s) 70, 306
TfiI GAWTC 2 cut(s) 4, 214
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TseI GCWGC 2 cut(s) 271, 300
TspDTI ATGAA 1 cut(s) 269
VpaK11BI GGWCC 2 cut(s) 76, 151
XagI CCTNNNNNAGG 1 cut(s) 171
XceI RCATGY 1 cut(s) 19
XspI CTAG 2 cut(s) 57, 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.