RCHIOBHM_CPG0502271

Binds 16S rRNA, required for the assembly of 30S particles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0027583
Physical Location & Seq
Forward (+)
75090 .. 75652
563 bp
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UTR
Exon/CDS
Intron
PRQ15710

Sequence Viewer

Length: 303 bp
ATGGCAAGAAAAAGTTTGATTCAGAGGGAGAAGAAGAGACAAAAATTGGAAAAAAAATATCATTTGATTCGTCGATCCTCAAAAAAAGAAATAAGCAAAGTTCCGTCGTTGAGCGAGAAATGGGAAATTCATGGAAAGTTACAATCTCCACCACGTAATAGTGCACCTACACGCCTTCATCGACGTTGTTTTTCGACCGGAAGACCGAGAGCTAACTATCGAGACTTTGGGTTATCCGGACACATACTTCGCGAAATGGTTCATGCATGTTTGTTGCCCGGGGCAACAAGATCAAGTTGGTAA

Protein Analysis

100

Amino Acids

11.73

Weight (kDa)

11.35

Isoelectric Point (pI)

77.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 46 - 99 8.4e-20 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0027583)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00330
rosa_chinensis RchiOBHm_CPg0502271

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 252
AccIII TCCGGA 1 cut(s) 236
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 1 cut(s) 126
AluBI AGCT 1 cut(s) 212
AluI AGCT 1 cut(s) 212
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 2 cut(s) 31, 216
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 1 cut(s) 69
Ama87I CYCGRG 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 236
ApaLI GTGCAC 1 cut(s) 162
ApoI RAATTY 1 cut(s) 126
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
Asp700I GAANNNNTTC 1 cut(s) 258
AsuC2I CCSGG 2 cut(s) 279, 280
AvaI CYCGRG 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 166
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 166
BcnI CCSGG 2 cut(s) 279, 280
BcoDI GTCTC 2 cut(s) 31, 216
Bme1390I CCNGG 2 cut(s) 279, 280
BmeT110I CYCGRG 1 cut(s) 278
BmrFI CCNGG 2 cut(s) 279, 280
BpiI GAAGAC 1 cut(s) 208
BpuMI CCSGG 2 cut(s) 279, 280
BsaAI YACGTR 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 278, 279
BsaWI WCCGGW 2 cut(s) 197, 236
BseAI TCCGGA 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 278, 279
BseSI GKGCMC 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 252
Bsh1285I CGRYCG 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 3 cut(s) 198, 237, 279
BsmAI GTCTC 2 cut(s) 31, 216
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 236
Bsp143I GATC 2 cut(s) 74, 290
Bsp68I TCGCGA 1 cut(s) 252
BspEI TCCGGA 1 cut(s) 236
BspFNI CGCG 1 cut(s) 252
BspPI GGATC 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 278, 279
BssMI GATC 2 cut(s) 74, 290
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 155
BstFNI CGCG 1 cut(s) 252
BstKTI GATC 2 cut(s) 77, 293
BstMAI GTCTC 2 cut(s) 31, 216
BstMBI GATC 2 cut(s) 74, 290
BstMCI CGRYCG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 270
BstSCI CCNGG 2 cut(s) 277, 278
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 208
BtuMI TCGCGA 1 cut(s) 252
Cfr9I CCCGGG 1 cut(s) 278
CviAII CATG 3 cut(s) 131, 263, 267
CviJI RGCY 1 cut(s) 212
CviKI_1 RGCY 1 cut(s) 212
DpnI GATC 2 cut(s) 76, 292
DpnII GATC 2 cut(s) 74, 290
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco88I CYCGRG 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 3 cut(s) 134, 266, 270
FaiI YATR 4 cut(s) 132, 245, 264, 268
FatI CATG 3 cut(s) 130, 262, 266
HapII CCGG 3 cut(s) 198, 237, 279
Hin1II CATG 3 cut(s) 134, 266, 270
HinfI GANTC 2 cut(s) 19, 67
HpaII CCGG 3 cut(s) 198, 237, 279
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 3 cut(s) 221, 237, 251
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 3 cut(s) 75, 109, 186
HpyAV CCTTC 1 cut(s) 185
HpyCH4IV ACGT 2 cut(s) 154, 184
HpyCH4V TGCA 2 cut(s) 164, 266
HpySE526I ACGT 2 cut(s) 154, 184
Hsp92II CATG 3 cut(s) 134, 266, 270
Kpn2I TCCGGA 1 cut(s) 236
Kzo9I GATC 2 cut(s) 74, 290
LpnPI CCDG 3 cut(s) 211, 250, 292
MaeII ACGT 2 cut(s) 154, 184
MaeIII GTNAC 1 cut(s) 138
MalI GATC 2 cut(s) 76, 292
MboI GATC 2 cut(s) 74, 290
MboII GAAGA 3 cut(s) 43, 46, 213
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 2 cut(s) 44, 126
MnlI CCTC 2 cut(s) 18, 88
Mph1103I ATGCAT 1 cut(s) 268
MroI TCCGGA 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 258
MspI CCGG 3 cut(s) 198, 237, 279
MspR9I CCNGG 2 cut(s) 279, 280
MvnI CGCG 1 cut(s) 252
NciI CCSGG 2 cut(s) 279, 280
NdeII GATC 2 cut(s) 74, 290
NlaIII CATG 3 cut(s) 134, 266, 270
NruI TCGCGA 1 cut(s) 252
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 19, 67
Ppu21I YACGTR 1 cut(s) 155
RruI TCGCGA 1 cut(s) 252
Sau3AI GATC 2 cut(s) 74, 290
ScrFI CCNGG 2 cut(s) 279, 280
SduI GDGCHC 1 cut(s) 166
SetI ASST 4 cut(s) 157, 169, 187, 214
SmaI CCCGGG 1 cut(s) 280
Sse9I AATT 2 cut(s) 44, 126
StyD4I CCNGG 2 cut(s) 277, 278
TaiI ACGT 2 cut(s) 157, 187
TaqI TCGA 4 cut(s) 73, 181, 194, 220
TaqII GACCGA 1 cut(s) 220
TasI AATT 2 cut(s) 44, 126
TfiI GAWTC 2 cut(s) 19, 67
TspDTI ATGAA 3 cut(s) 119, 167, 251
TspGWI ACGGA 1 cut(s) 93
TspMI CCCGGG 1 cut(s) 278
VneI GTGCAC 1 cut(s) 162
XapI RAATTY 1 cut(s) 126
XceI RCATGY 1 cut(s) 270
XmaI CCCGGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.