RchiOBHm_Chr1g0333661

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0029640
Physical Location & Seq
Forward (+)
25795937 .. 25796563
627 bp
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UTR
Exon/CDS
Intron
PRQ56246

Sequence Viewer

Length: 627 bp
ATGAAGGTACTCCTTTCGTACGAGTCAACTGCCTACTTTCAGATGACAACATCCCAACCGGACCCAAACCAACTCAACAAGCTACTCCCATGTGAGTTGGAATGTGTTGTCGACTTTGTTCTTGCAGTCCAAGCCAACATCTTTGACTGTGGTGGCATGACTATCGGAGTGTGCATCTCGCACAAAATCGCCGACGCCTTGTCACTGGTCATGTTTCTAAACCCTTGGGCTTCCGTTTCCCGACGAGATAATTGTAATTACACAGCTCCTGCTTTTGACTTGGCTACGTTCTTCCCGCGGAGATCCATATCCGGGTTCAAACCAGGGACTGGAATCGCAAGAGACAAAATCGTTACTAAACGATTCGTTTTCAGCGCCTCCACCGTTGCTTCTCTCCTAACAAAATACACGACATCTGCAACTGACAGACGCCCTACTCGTATCGAGGCTCTGTCAGCATTTGTATGGAGCCGTTTCTTGGCTTCCATGCAAGGAAAGCCCAACTCGGACCTGGTTTACAAAGTTCTCCACGCATGCAGTCAACCTACGAACCAGAATGGACCAGGCGCTTCCAGAGCATCATTTCGGAAACGTGAGCCGGTTCGCGAAGGCGACGACGTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

208

Amino Acids

22.96

Weight (kDa)

9.23

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 21 - 168 1.5e-23 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029640)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0333661
rosa_multiflora Rmu_sc0011237.1_g000012

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 621
AccB7I CCANNNNNTGG 1 cut(s) 329
AccI GTMKAC 1 cut(s) 111
AccII CGCG 2 cut(s) 298, 606
AciI CCGC 2 cut(s) 296, 298
AclWI GGATC 1 cut(s) 297
AcyI GRCGYC 3 cut(s) 195, 430, 618
AfaI GTAC 2 cut(s) 9, 20
AfiI CCNNNNNNNGG 3 cut(s) 312, 329, 478
AgsI TTSAA 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 199
AjnI CCWGG 3 cut(s) 322, 510, 562
AluBI AGCT 2 cut(s) 82, 266
AluI AGCT 2 cut(s) 82, 266
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 1 cut(s) 297
AlwNI CAGNNNCTG 2 cut(s) 269, 329
AspLEI GCGC 2 cut(s) 377, 569
AspS9I GGNCC 3 cut(s) 61, 508, 560
AsuC2I CCSGG 1 cut(s) 313
AvaII GGWCC 3 cut(s) 61, 508, 560
BceAI ACGGC 1 cut(s) 456
BcgI CGANNNNNNTGC 4 cut(s) 11, 45, 145, 179
BciT130I CCWGG 3 cut(s) 324, 512, 564
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 336
BfoI RGCGCY 2 cut(s) 378, 570
Bme1390I CCNGG 4 cut(s) 313, 324, 512, 564
Bme18I GGWCC 3 cut(s) 61, 508, 560
BmeRI GACNNNNNGTC 1 cut(s) 199
BmgT120I GGNCC 3 cut(s) 61, 508, 560
BmiI GGNNCC 2 cut(s) 63, 470
BmrFI CCNGG 4 cut(s) 313, 324, 512, 564
BmsI GCATC 2 cut(s) 183, 587
BpuMI CCSGG 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 307
BsaHI GRCGYC 3 cut(s) 195, 430, 618
BsaJI CCNNGG 3 cut(s) 224, 296, 323
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 329, 478
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 2 cut(s) 210, 334
Bse8I GATNNNNATC 1 cut(s) 307
BseBI CCWGG 3 cut(s) 324, 512, 564
BseDI CCNNGG 3 cut(s) 224, 296, 323
BseGI GGATG 1 cut(s) 50
BseJI GATNNNNATC 1 cut(s) 307
BseLI CCNNNNNNNGG 3 cut(s) 312, 329, 478
BseNI ACTGG 2 cut(s) 210, 334
Bsh1236I CGCG 2 cut(s) 298, 606
BsiSI CCGG 3 cut(s) 59, 312, 599
BsiWI CGTACG 1 cut(s) 18
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 312, 329, 478
BsmAI GTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 340
Bsp143I GATC 1 cut(s) 302
Bsp68I TCGCGA 1 cut(s) 606
BspACI CCGC 2 cut(s) 296, 298
BspFNI CGCG 2 cut(s) 298, 606
BspLI GGNNCC 2 cut(s) 63, 470
BspPI GGATC 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 2 cut(s) 210, 334
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 3 cut(s) 224, 296, 323
BssMI GATC 1 cut(s) 302
BssNI GRCGYC 3 cut(s) 195, 430, 618
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 3 cut(s) 324, 512, 564
Bst4CI ACNGT 2 cut(s) 149, 385
BstACI GRCGYC 3 cut(s) 195, 430, 618
BstC8I GCNNGC 1 cut(s) 535
BstDSI CCRYGG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 50
BstFNI CGCG 2 cut(s) 298, 606
BstH2I RGCGCY 2 cut(s) 378, 570
BstHHI GCGC 2 cut(s) 377, 569
BstKTI GATC 1 cut(s) 305
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 1 cut(s) 302
BstMWI GCNNNNNNNGC 4 cut(s) 131, 455, 496, 575
BstNI CCWGG 3 cut(s) 324, 512, 564
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 4 cut(s) 311, 322, 510, 562
BstUI CGCG 2 cut(s) 298, 606
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BtgI CCRYGG 1 cut(s) 296
BtsCI GGATG 1 cut(s) 50
BtsIMutI CAGTG 1 cut(s) 203
BtuMI TCGCGA 1 cut(s) 606
Cac8I GCNNGC 1 cut(s) 535
CaiI CAGNNNCTG 2 cut(s) 269, 329
CfoI GCGC 2 cut(s) 377, 569
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 3 cut(s) 61, 508, 560
Cfr42I CCGCGG 1 cut(s) 299
CseI GACGC 2 cut(s) 203, 438
CsiI ACCWGGT 1 cut(s) 510
Csp6I GTAC 2 cut(s) 8, 19
CviAII CATG 5 cut(s) 90, 157, 211, 487, 534
CviQI GTAC 2 cut(s) 8, 19
DpnI GATC 1 cut(s) 304
DpnII GATC 1 cut(s) 302
DriI GACNNNNNGTC 1 cut(s) 199
Eam1105I GACNNNNNGTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 224
Eco47I GGWCC 3 cut(s) 61, 508, 560
EcoRII CCWGG 3 cut(s) 322, 510, 562
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 5 cut(s) 93, 160, 214, 490, 537
FaiI YATR 7 cut(s) 91, 158, 212, 308, 466, 488, 535
FaqI GGGAC 1 cut(s) 340
FatI CATG 5 cut(s) 89, 156, 210, 486, 533
FauI CCCGC 1 cut(s) 303
FblI GTMKAC 1 cut(s) 111
FokI GGATG 1 cut(s) 37
GlaI GCGC 2 cut(s) 376, 568
HaeII RGCGCY 2 cut(s) 378, 570
HapII CCGG 3 cut(s) 59, 312, 599
HgaI GACGC 2 cut(s) 203, 438
HhaI GCGC 2 cut(s) 377, 569
Hin1I GRCGYC 3 cut(s) 195, 430, 618
Hin1II CATG 5 cut(s) 93, 160, 214, 490, 537
Hin6I GCGC 2 cut(s) 375, 567
HinP1I GCGC 2 cut(s) 375, 567
HincII GTYRAC 3 cut(s) 27, 112, 542
HindII GTYRAC 3 cut(s) 27, 112, 542
HinfI GANTC 3 cut(s) 23, 333, 363
HpaII CCGG 3 cut(s) 59, 312, 599
Hpy166II GTNNAC 4 cut(s) 27, 112, 517, 542
Hpy188I TCNGA 4 cut(s) 42, 167, 508, 588
Hpy188III TCNNGA 3 cut(s) 240, 573, 605
Hpy8I GTNNAC 4 cut(s) 27, 112, 517, 542
Hpy99I CGWCG 5 cut(s) 197, 246, 617, 620, 623
HpyAV CCTTC 1 cut(s) 602
HpyCH4III ACNGT 2 cut(s) 149, 385
HpyCH4IV ACGT 3 cut(s) 287, 592, 618
HpyCH4V TGCA 5 cut(s) 125, 174, 419, 490, 537
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 455, 496, 575
HpySE526I ACGT 3 cut(s) 287, 592, 618
Hsp92I GRCGYC 3 cut(s) 195, 430, 618
Hsp92II CATG 5 cut(s) 93, 160, 214, 490, 537
HspAI GCGC 2 cut(s) 375, 567
KspI CCGCGG 1 cut(s) 299
Kzo9I GATC 1 cut(s) 302
LmnI GCTCC 2 cut(s) 271, 468
LweI GCATC 2 cut(s) 183, 587
MabI ACCWGGT 1 cut(s) 510
MaeII ACGT 3 cut(s) 287, 592, 618
MaeIII GTNAC 2 cut(s) 201, 352
MalI GATC 1 cut(s) 304
MboI GATC 1 cut(s) 302
MboII GAAGA 1 cut(s) 283
MflI RGATCY 1 cut(s) 302
MluCI AATT 2 cut(s) 250, 256
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 2 cut(s) 388, 439
MslI CAYNNNNRTG 1 cut(s) 463
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 3 cut(s) 59, 312, 599
MspR9I CCNGG 4 cut(s) 313, 324, 512, 564
MvaI CCWGG 3 cut(s) 324, 512, 564
MvnI CGCG 2 cut(s) 298, 606
MwoI GCNNNNNNNGC 4 cut(s) 131, 455, 496, 575
NciI CCSGG 1 cut(s) 313
NdeII GATC 1 cut(s) 302
NlaIII CATG 5 cut(s) 93, 160, 214, 490, 537
NlaIV GGNNCC 2 cut(s) 63, 470
NmuCI GTSAC 1 cut(s) 201
NruI TCGCGA 1 cut(s) 606
NspI RCATGY 1 cut(s) 537
PaeI GCATGC 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 436
PfeI GAWTC 2 cut(s) 333, 363
Pfl23II CGTACG 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 617
PflMI CCANNNNNTGG 1 cut(s) 329
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Psp6I CCWGG 3 cut(s) 322, 510, 562
PspGI CCWGG 3 cut(s) 322, 510, 562
PspLI CGTACG 1 cut(s) 18
PspN4I GGNNCC 2 cut(s) 63, 470
PspPI GGNCC 3 cut(s) 61, 508, 560
PstNI CAGNNNCTG 2 cut(s) 269, 329
PsuI RGATCY 1 cut(s) 302
PsyI GACNNNGTC 1 cut(s) 617
RruI TCGCGA 1 cut(s) 606
RsaI GTAC 2 cut(s) 9, 20
RsaNI GTAC 2 cut(s) 8, 19
RseI CAYNNNNRTG 1 cut(s) 463
SacII CCGCGG 1 cut(s) 299
SalI GTCGAC 1 cut(s) 110
Sau3AI GATC 1 cut(s) 302
Sau96I GGNCC 3 cut(s) 61, 508, 560
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 4 cut(s) 313, 324, 512, 564
SetI ASST 8 cut(s) 9, 84, 268, 290, 513, 547, 595, 621
SexAI ACCWGGT 1 cut(s) 510
SfaNI GCATC 2 cut(s) 183, 587
Sfr303I CCGCGG 1 cut(s) 299
SgrBI CCGCGG 1 cut(s) 299
SinI GGWCC 3 cut(s) 61, 508, 560
SmiMI CAYNNNNRTG 1 cut(s) 463
SphI GCATGC 1 cut(s) 537
Sse9I AATT 2 cut(s) 250, 256
SsiI CCGC 2 cut(s) 296, 298
StyD4I CCNGG 4 cut(s) 311, 322, 510, 562
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 2 cut(s) 149, 385
TaiI ACGT 3 cut(s) 290, 595, 621
TaqI TCGA 2 cut(s) 111, 444
TasI AATT 2 cut(s) 250, 256
TfiI GAWTC 2 cut(s) 333, 363
TscAI CASTG 1 cut(s) 210
TseFI GTSAC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 201
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 1 cut(s) 210
Tth111I GACNNNGTC 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 329
VpaK11BI GGWCC 3 cut(s) 61, 508, 560
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 111
ZraI GACGTC 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.