RchiOBHm_Chr1g0366831
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0029620
Physical Location & Seq
Forward (+)
57281896 .. 57285732
3837 bp
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UTR
Exon/CDS
Intron
PRQ59136

Sequence Viewer

Length: 516 bp
ATGACTATGCTTGATGGAATATATCAAGCTGCCAAGGGTGTCCCCCAGTTACAGACCTGCTTTAGCATTGATGCCAATGATATCTTCAGTGTCTCTGCTGAGGATATTAACAGCGACCACATGAAAGGGATTATAAATAACAAAGGCAGAACAAGTTTTGAGGGAATTGGGAAGATGAGGAGTTTTTTTCAGCTGAGAATGGCAAGAGAACTAGATAACCATGCAATTGGAATTGACCTCGGAACAACCTACTCGTGCGTGGCGGTGTGGCAGCAGGACCATGTAGAAGTGATACTGAACGATCAAGGAAACAGGACCACTCCATCCTATGTTGCTTTTACCAAGACTGAGCAGTTAATAGGTGACGCAGCATTTAACCAAGTCATCAGAAACCCGGCTAACTCCATCTTTGATGCAAAGAGATTGATTGGTAGAAGATTCAGTGACACCTCTGTTCAAATGGGAAGCTCTGGCCCTTCAAGGTTACTGAAGGTCCTGGTGATAAACCCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

171

Amino Acids

18.88

Weight (kDa)

6.82

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 75 - 154 4.8e-33 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029620)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0366781 RchiOBHm_Chr1g0366831

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 65
AciI CCGC 1 cut(s) 263
AcuI CTGAAG 2 cut(s) 70, 509
AgsI TTSAA 2 cut(s) 458, 480
AjnI CCWGG 1 cut(s) 495
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AluBI AGCT 3 cut(s) 29, 193, 468
AluI AGCT 3 cut(s) 29, 193, 468
Alw26I GTCTC 1 cut(s) 97
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 3 cut(s) 29, 271, 368
AspS9I GGNCC 4 cut(s) 277, 315, 473, 493
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 2 cut(s) 374, 511
AvaII GGWCC 3 cut(s) 277, 315, 493
BauI CACGAG 1 cut(s) 253
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 3 cut(s) 16, 283, 380
BccI CCATC 3 cut(s) 8, 331, 413
BciT130I CCWGG 1 cut(s) 497
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 212
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 3 cut(s) 30, 272, 369
BlsI GCNGC 3 cut(s) 31, 273, 370
Bme1390I CCNGG 2 cut(s) 395, 497
Bme18I GGWCC 3 cut(s) 277, 315, 493
BmgT120I GGNCC 4 cut(s) 277, 315, 473, 493
BmrFI CCNGG 2 cut(s) 395, 497
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 2 cut(s) 61, 403
BmuI ACTGGG 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 33, 238
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 2 cut(s) 33, 238
BseGI GGATG 1 cut(s) 323
BseMII CTCAG 3 cut(s) 90, 185, 339
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 3 cut(s) 16, 283, 380
BshFI GGCC 1 cut(s) 474
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 1 cut(s) 474
Bsp143I GATC 1 cut(s) 301
BspACI CCGC 1 cut(s) 263
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 3 cut(s) 91, 186, 340
BspMI ACCTGC 1 cut(s) 65
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 33, 238
BssMI GATC 1 cut(s) 301
BssSI CACGAG 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 497
BstDEI CTNAG 3 cut(s) 99, 194, 348
BstF5I GGATG 1 cut(s) 323
BstKTI GATC 1 cut(s) 304
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 1 cut(s) 301
BstNI CCWGG 1 cut(s) 497
BstSCI CCNGG 2 cut(s) 393, 495
BstV1I GCAGC 3 cut(s) 16, 283, 380
BstXI CCANNNNNNTGG 1 cut(s) 227
BsuRI GGCC 1 cut(s) 474
BtsCI GGATG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 94, 448
BveI ACCTGC 1 cut(s) 65
Cfr13I GGNCC 4 cut(s) 277, 315, 473, 493
CseI GACGC 1 cut(s) 374
CviAII CATG 3 cut(s) 121, 221, 281
CviJI RGCY 5 cut(s) 29, 193, 398, 468, 474
CviKI_1 RGCY 5 cut(s) 29, 193, 398, 468, 474
DdeI CTNAG 3 cut(s) 99, 194, 348
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 33
Eco32I GATATC 1 cut(s) 82
Eco47I GGWCC 3 cut(s) 277, 315, 493
Eco57I CTGAAG 2 cut(s) 70, 509
EcoO109I RGGNCCY 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 3 cut(s) 124, 224, 284
FaiI YATR 8 cut(s) 8, 22, 122, 134, 222, 282, 330, 514
FaqI GGGAC 1 cut(s) 26
FatI CATG 3 cut(s) 120, 220, 280
Fnu4HI GCNGC 3 cut(s) 30, 272, 369
FokI GGATG 1 cut(s) 310
Fsp4HI GCNGC 3 cut(s) 30, 272, 369
FspBI CTAG 1 cut(s) 212
GluI GCNGC 3 cut(s) 30, 272, 369
HaeIII GGCC 1 cut(s) 474
HapII CCGG 1 cut(s) 395
HgaI GACGC 1 cut(s) 374
Hin1II CATG 3 cut(s) 124, 224, 284
HinfI GANTC 1 cut(s) 438
HpaII CCGG 1 cut(s) 395
HphI GGTGA 2 cut(s) 374, 511
Hpy188I TCNGA 2 cut(s) 242, 389
HpyAV CCTTC 2 cut(s) 484, 486
HpyCH4V TGCA 2 cut(s) 224, 416
HpyF3I CTNAG 3 cut(s) 99, 194, 348
Hsp92II CATG 3 cut(s) 124, 224, 284
Kzo9I GATC 1 cut(s) 301
LpnPI CCDG 8 cut(s) 59, 70, 260, 298, 408, 456, 482, 509
Lsp1109I GCAGC 3 cut(s) 16, 283, 380
LweI GCATC 2 cut(s) 61, 403
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 4 cut(s) 48, 362, 443, 483
MalI GATC 1 cut(s) 303
MboI GATC 1 cut(s) 301
MboII GAAGA 3 cut(s) 76, 184, 447
MfeI CAATTG 1 cut(s) 225
MluCI AATT 3 cut(s) 165, 225, 231
MnlI CCTC 5 cut(s) 94, 154, 171, 248, 460
MseI TTAA 3 cut(s) 108, 356, 375
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 395, 497
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 497
NciI CCSGG 1 cut(s) 395
NdeII GATC 1 cut(s) 301
NlaIII CATG 3 cut(s) 124, 224, 284
NmuCI GTSAC 2 cut(s) 362, 443
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 3 cut(s) 31, 273, 370
PpuMI RGGWCCY 1 cut(s) 493
PsiI TTATAA 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspPI GGNCC 4 cut(s) 277, 315, 473, 493
PspPPI RGGWCCY 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 193
SaqAI TTAA 3 cut(s) 108, 356, 375
SatI GCNGC 3 cut(s) 30, 272, 369
Sau3AI GATC 1 cut(s) 301
Sau96I GGNCC 4 cut(s) 277, 315, 473, 493
ScrFI CCNGG 2 cut(s) 395, 497
SfaNI GCATC 2 cut(s) 61, 403
SinI GGWCC 3 cut(s) 277, 315, 493
Sse9I AATT 3 cut(s) 165, 225, 231
SsiI CCGC 1 cut(s) 263
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 393, 495
StyI CCWWGG 1 cut(s) 33
TasI AATT 3 cut(s) 165, 225, 231
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 3 cut(s) 108, 356, 375
Tru9I TTAA 3 cut(s) 108, 356, 375
TscAI CASTG 2 cut(s) 94, 448
TseFI GTSAC 2 cut(s) 362, 443
TseI GCWGC 3 cut(s) 29, 271, 368
Tsp45I GTSAC 2 cut(s) 362, 443
TspDTI ATGAA 1 cut(s) 137
TspRI CASTG 2 cut(s) 94, 448
VpaK11BI GGWCC 3 cut(s) 277, 315, 493
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.