RchiOBHm_Chr2g0141131

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0022689
Physical Location & Seq
Reverse (-)
58699199 .. 58699621
423 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51146

Sequence Viewer

Length: 423 bp
ATGCCACTGACCCAATACCAACCCCCAAATCCCGTCCATGACCCTTGGCCCTTTTGCTCCATCCCGTCGCAATCTAAAACTGCAACGTTCACCCACGAGCACATCGACTGGAAGGAGACGCTGACTGCGCACATGTCCAAGGCTGACTTGCTGGCCCTGCAGAAGGACGAGGTCAAGGTGGAGGTGGAGGACGGCAAGATCCTTCAGGTCATCGGAGAGTGGAAGCTCGATGTGGAAGAGAAGACCGAGACGTGGCACCGGGTCGAGCGCAGCAGCAGCAGGTTCACAAGGCGGTTCAGGCTGCCGGAGAATGTGAGAGTTGAGCACGTCCAGGCGGCGATGGAGAACGGCGTCCTCAACGTCACGGTGCCCAAGGAGGAGGCCAAGAAGGTCTATGTCAGGTCAATTGAGATCTCTCGTTAA

Protein Analysis

140

Amino Acids

16.32

Weight (kDa)

6.31

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 36 - 139 3.2e-24 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022689)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0141131
rosa_samantha Rh2DG438100
rosa_wichuraiana Rw0G019780 Rw7G007310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 270
AccB1I GGYRCC 2 cut(s) 255, 367
AciI CCGC 2 cut(s) 292, 335
AclI AACGTT 1 cut(s) 86
AclWI GGATC 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 351
AfiI CCNNNNNNNGG 2 cut(s) 163, 252
AflIII ACRYGT 1 cut(s) 132
AjiI CACGTC 2 cut(s) 252, 328
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw21I GWGCWC 2 cut(s) 102, 327
Alw26I GTCTC 2 cut(s) 110, 242
AlwI GGATC 1 cut(s) 193
AoxI GGCC 3 cut(s) 47, 153, 381
ApeKI GCWGC 4 cut(s) 270, 273, 276, 301
AspLEI GCGC 2 cut(s) 130, 270
AspS9I GGNCC 2 cut(s) 48, 154
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 372
BanI GGYRCC 2 cut(s) 255, 367
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 102, 327
BbvI GCAGC 4 cut(s) 282, 285, 288, 288
BccI CCATC 2 cut(s) 68, 334
BceAI ACGGC 2 cut(s) 208, 364
BciT130I CCWGG 1 cut(s) 332
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 2 cut(s) 110, 242
BfmI CTRYAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 270
BglII AGATCT 1 cut(s) 411
BisI GCNGC 5 cut(s) 271, 274, 277, 302, 336
BlsI GCNGC 5 cut(s) 272, 275, 278, 303, 337
Bme1390I CCNGG 2 cut(s) 260, 332
BmgBI CACGTC 2 cut(s) 252, 328
BmgT120I GGNCC 2 cut(s) 48, 154
BmiI GGNNCC 2 cut(s) 257, 369
BmrFI CCNGG 2 cut(s) 260, 332
BpiI GAAGAC 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 260
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 3 cut(s) 44, 138, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 252
Bse1I ACTGG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 3 cut(s) 44, 138, 372
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 163, 252
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 392
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 4 cut(s) 282, 285, 288, 288
BshFI GGCC 3 cut(s) 49, 155, 383
BshNI GGYRCC 2 cut(s) 255, 367
BsiHKAI GWGCWC 2 cut(s) 102, 327
BsiSI CCGG 2 cut(s) 259, 305
BslI CCNNNNNNNGG 2 cut(s) 163, 252
BsmAI GTCTC 2 cut(s) 110, 242
BsmBI CGTCTC 2 cut(s) 110, 242
BsnI GGCC 3 cut(s) 49, 155, 383
Bsp1286I GDGCHC 3 cut(s) 102, 327, 372
Bsp143I GATC 2 cut(s) 198, 411
BspACI CCGC 2 cut(s) 292, 335
BspANI GGCC 3 cut(s) 49, 155, 383
BspLI GGNNCC 2 cut(s) 257, 369
BspMAI CTGCAG 1 cut(s) 162
BspMI ACCTGC 1 cut(s) 270
BspPI GGATC 1 cut(s) 193
BspT107I GGYRCC 2 cut(s) 255, 367
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 44, 138, 372
BssMI GATC 2 cut(s) 198, 411
BssNI GRCGYC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 3 cut(s) 44, 138, 372
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 367
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 1 cut(s) 351
BstC8I GCNNGC 1 cut(s) 153
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 60
BstHHI GCGC 2 cut(s) 130, 270
BstKTI GATC 2 cut(s) 201, 414
BstMAI GTCTC 2 cut(s) 110, 242
BstMBI GATC 2 cut(s) 198, 411
BstMWI GCNNNNNNNGC 4 cut(s) 127, 157, 276, 298
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 258, 330
BstSFI CTRYAG 1 cut(s) 158
BstSLI GKGCMC 1 cut(s) 372
BstV1I GCAGC 4 cut(s) 282, 285, 288, 288
BstV2I GAAGAC 1 cut(s) 248
BstX2I RGATCY 2 cut(s) 198, 411
BstYI RGATCY 2 cut(s) 198, 411
BsuRI GGCC 3 cut(s) 49, 155, 383
BtgZI GCGATG 1 cut(s) 353
BtrI CACGTC 2 cut(s) 252, 328
BtsCI GGATG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 5
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 2 cut(s) 130, 270
Cfr13I GGNCC 2 cut(s) 48, 154
CseI GACGC 2 cut(s) 127, 340
CviAII CATG 2 cut(s) 38, 133
CviJI RGCY 6 cut(s) 49, 143, 155, 226, 301, 383
CviKI_1 RGCY 6 cut(s) 49, 143, 155, 226, 301, 383
DpnI GATC 2 cut(s) 200, 413
DpnII GATC 2 cut(s) 198, 411
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco130I CCWWGG 3 cut(s) 44, 138, 372
Eco57I CTGAAG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 3 cut(s) 44, 138, 372
ErhI CCWWGG 3 cut(s) 44, 138, 372
Esp3I CGTCTC 2 cut(s) 110, 242
FaeI CATG 2 cut(s) 41, 136
FaiI YATR 3 cut(s) 39, 134, 396
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FatI CATG 2 cut(s) 37, 132
Fnu4HI GCNGC 5 cut(s) 271, 274, 277, 302, 336
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 5 cut(s) 271, 274, 277, 302, 336
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 2 cut(s) 129, 269
GluI GCNGC 5 cut(s) 271, 274, 277, 302, 336
HaeIII GGCC 3 cut(s) 49, 155, 383
HapII CCGG 2 cut(s) 259, 305
HgaI GACGC 2 cut(s) 127, 340
HhaI GCGC 2 cut(s) 130, 270
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 2 cut(s) 41, 136
Hin6I GCGC 2 cut(s) 128, 268
HinP1I GCGC 2 cut(s) 128, 268
HpaII CCGG 2 cut(s) 259, 305
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 90, 285
Hpy188I TCNGA 1 cut(s) 215
Hpy8I GTNNAC 2 cut(s) 90, 285
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 4 cut(s) 106, 157, 212, 382
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 4 cut(s) 86, 251, 327, 360
HpyCH4V TGCA 2 cut(s) 83, 160
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 157, 276, 298
HpySE526I ACGT 4 cut(s) 86, 251, 327, 360
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 2 cut(s) 41, 136
HspAI GCGC 2 cut(s) 128, 268
Kzo9I GATC 2 cut(s) 198, 411
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 4 cut(s) 282, 285, 288, 288
MaeII ACGT 4 cut(s) 86, 251, 327, 360
MaeIII GTNAC 1 cut(s) 361
MalI GATC 2 cut(s) 200, 413
MboI GATC 2 cut(s) 198, 411
MboII GAAGA 2 cut(s) 248, 253
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 198, 411
MhlI GDGCHC 3 cut(s) 102, 327, 372
MluCI AATT 1 cut(s) 405
MnlI CCTC 6 cut(s) 163, 175, 181, 365, 370, 373
MseI TTAA 1 cut(s) 421
MspI CCGG 2 cut(s) 259, 305
MspR9I CCNGG 2 cut(s) 260, 332
MunI CAATTG 1 cut(s) 405
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 127, 157, 276, 298
NciI CCSGG 1 cut(s) 260
NdeII GATC 2 cut(s) 198, 411
NlaIII CATG 2 cut(s) 41, 136
NlaIV GGNNCC 2 cut(s) 257, 369
NmuCI GTSAC 1 cut(s) 361
NsbI TGCGCA 1 cut(s) 129
NspI RCATGY 1 cut(s) 136
PciI ACATGT 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 5 cut(s) 272, 275, 278, 303, 337
PscI ACATGT 1 cut(s) 132
Psp1406I AACGTT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 2 cut(s) 257, 369
PspPI GGNCC 2 cut(s) 48, 154
PstI CTGCAG 1 cut(s) 162
PsuI RGATCY 2 cut(s) 198, 411
PsyI GACNNNGTC 1 cut(s) 170
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 5 cut(s) 271, 274, 277, 302, 336
Sau3AI GATC 2 cut(s) 198, 411
Sau96I GGNCC 2 cut(s) 48, 154
ScrFI CCNGG 2 cut(s) 260, 332
SduI GDGCHC 3 cut(s) 102, 327, 372
SfcI CTRYAG 1 cut(s) 158
Sse9I AATT 1 cut(s) 405
SsiI CCGC 2 cut(s) 292, 335
StyD4I CCNGG 2 cut(s) 258, 330
StyI CCWWGG 3 cut(s) 44, 138, 372
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 4 cut(s) 89, 254, 330, 363
TaqI TCGA 3 cut(s) 105, 228, 264
TaqII GACCGA 1 cut(s) 260
TasI AATT 1 cut(s) 405
TauI GCSGC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 270, 273, 276, 301
Tsp45I GTSAC 1 cut(s) 361
TspRI CASTG 1 cut(s) 12
Tth111I GACNNNGTC 1 cut(s) 170
XagI CCTNNNNNAGG 1 cut(s) 161
XceI RCATGY 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.