Rw7G007310

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0022689
Physical Location & Seq
Reverse (-)
6308067 .. 6308426
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G007310.1

Sequence Viewer

Length: 360 bp
ATGTCGCAATCTGAAACGGCGTTGTTCACCCACGCGCACATCAACTGGAAGGAGACGCTTACGGCGCACGTGTCCAAGGCTGATGTGCCGGGCCTGCGGAAGGACGAGGTCAAGGTGGAGGTGGAGGATGGCAAGATTCTTCAGGTCATCGGAGAGCGGAAGCACGTTGCAGAAGAGAAGACCGAGACGTGGCACCGGGTAGAGCACAGCAGCAGCAGGTTCACGAGGAGGTTTAGGCTGTCGGAGAATGCGAGAGTCGAGCAGGTCCAGGCTGCGATGGAGAACGGCGTCCTTAACGTCACAGTACCCAAGGAGGAGGCCAAGAAGGTCTATGTCAGGTCAATTGAGATCTCTAGTTAA

Protein Analysis

119

Amino Acids

13.62

Weight (kDa)

7.03

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 16 - 118 8.9e-26 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022689)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0141131
rosa_samantha Rh2DG438100
rosa_wichuraiana Rw0G019780 Rw7G007310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 207, 253
AccB1I GGYRCC 1 cut(s) 192
AccBSI CCGCTC 1 cut(s) 157
AccII CGCG 1 cut(s) 35
AciI CCGC 2 cut(s) 97, 157
AcuI CTGAAG 1 cut(s) 125
AcvI CACGTG 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 2 cut(s) 100, 189
AflIII ACRYGT 1 cut(s) 69
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 1 cut(s) 267
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 47, 179
AoxI GGCC 2 cut(s) 91, 318
ApeKI GCWGC 3 cut(s) 210, 213, 272
AspLEI GCGC 2 cut(s) 37, 67
AspS9I GGNCC 2 cut(s) 91, 265
AsuC2I CCSGG 2 cut(s) 90, 197
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 192
BauI CACGAG 1 cut(s) 223
BbrPI CACGTG 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 3 cut(s) 222, 225, 259
BccI CCATC 2 cut(s) 122, 271
BceAI ACGGC 3 cut(s) 33, 78, 301
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 2 cut(s) 90, 197
BcoDI GTCTC 2 cut(s) 47, 179
BfaI CTAG 1 cut(s) 354
BfuAI ACCTGC 2 cut(s) 207, 253
BglII AGATCT 1 cut(s) 348
BisI GCNGC 3 cut(s) 211, 214, 273
BlsI GCNGC 3 cut(s) 212, 215, 274
Bme1390I CCNGG 3 cut(s) 90, 197, 269
Bme18I GGWCC 1 cut(s) 265
BmgBI CACGTC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 91, 265
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 3 cut(s) 90, 197, 269
BpiI GAAGAC 1 cut(s) 185
BpuMI CCSGG 2 cut(s) 90, 197
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 75, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 189
Bse1I ACTGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 75, 309
BseGI GGATG 1 cut(s) 133
BseLI CCNNNNNNNGG 2 cut(s) 100, 189
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 241, 329
BseXI GCAGC 3 cut(s) 222, 225, 259
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 93, 320
BshNI GGYRCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 2 cut(s) 89, 196
BslI CCNNNNNNNGG 2 cut(s) 100, 189
BsmAI GTCTC 2 cut(s) 47, 179
BsmBI CGTCTC 2 cut(s) 47, 179
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 2 cut(s) 93, 320
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 1 cut(s) 348
BspACI CCGC 2 cut(s) 97, 157
BspANI GGCC 2 cut(s) 93, 320
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 1 cut(s) 194
BspMI ACCTGC 2 cut(s) 207, 253
BspT107I GGYRCC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 157
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 75, 309
BssMI GATC 1 cut(s) 348
BssNI GRCGYC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 223
BssT1I CCWWGG 2 cut(s) 75, 309
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 168
BstACI GRCGYC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 95
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 1 cut(s) 133
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 2 cut(s) 37, 67
BstKTI GATC 1 cut(s) 351
BstMAI GTCTC 2 cut(s) 47, 179
BstMBI GATC 1 cut(s) 348
BstMWI GCNNNNNNNGC 2 cut(s) 64, 94
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 3 cut(s) 88, 195, 267
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 222, 225, 259
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 2 cut(s) 93, 320
BtgZI GCGATG 1 cut(s) 290
BtrI CACGTC 1 cut(s) 189
BtsCI GGATG 1 cut(s) 133
BveI ACCTGC 2 cut(s) 207, 253
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 2 cut(s) 37, 67
Cfr13I GGNCC 2 cut(s) 91, 265
CseI GACGC 2 cut(s) 64, 277
Csp6I GTAC 1 cut(s) 305
CviJI RGCY 5 cut(s) 80, 93, 238, 272, 320
CviKI_1 RGCY 5 cut(s) 80, 93, 238, 272, 320
CviQI GTAC 1 cut(s) 305
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 75, 309
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 125
Eco72I CACGTG 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 75, 309
ErhI CCWWGG 2 cut(s) 75, 309
Esp3I CGTCTC 2 cut(s) 47, 179
FaiI YATR 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 211, 214, 273
FokI GGATG 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 211, 214, 273
FspBI CTAG 1 cut(s) 354
GlaI GCGC 2 cut(s) 36, 66
GluI GCNGC 3 cut(s) 211, 214, 273
HaeIII GGCC 2 cut(s) 93, 320
HapII CCGG 2 cut(s) 89, 196
HgaI GACGC 2 cut(s) 64, 277
HhaI GCGC 2 cut(s) 37, 67
Hin1I GRCGYC 1 cut(s) 288
Hin6I GCGC 2 cut(s) 35, 65
HinP1I GCGC 2 cut(s) 35, 65
HinfI GANTC 2 cut(s) 136, 255
HpaII CCGG 2 cut(s) 89, 196
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 27, 222
Hpy188I TCNGA 3 cut(s) 13, 152, 244
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 2 cut(s) 27, 222
HpyAV CCTTC 3 cut(s) 43, 94, 319
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 4 cut(s) 69, 165, 188, 297
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 94
HpySE526I ACGT 4 cut(s) 69, 165, 188, 297
Hsp92I GRCGYC 1 cut(s) 288
HspAI GCGC 2 cut(s) 35, 65
Kzo9I GATC 1 cut(s) 348
Lsp1109I GCAGC 3 cut(s) 222, 225, 259
MaeI CTAG 1 cut(s) 354
MaeII ACGT 4 cut(s) 69, 165, 188, 297
MaeIII GTNAC 1 cut(s) 298
MalI GATC 1 cut(s) 350
MbiI CCGCTC 1 cut(s) 157
MboI GATC 1 cut(s) 348
MboII GAAGA 3 cut(s) 131, 185, 190
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 1 cut(s) 342
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 7 cut(s) 100, 112, 118, 219, 222, 307, 310
MseI TTAA 2 cut(s) 294, 358
MspI CCGG 2 cut(s) 89, 196
MspR9I CCNGG 3 cut(s) 90, 197, 269
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 64, 94
NciI CCSGG 2 cut(s) 90, 197
NdeII GATC 1 cut(s) 348
NlaIV GGNNCC 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 136
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 212, 215, 274
PleI GAGTC 1 cut(s) 263
PmaCI CACGTG 1 cut(s) 70
PmlI CACGTG 1 cut(s) 70
PpsI GAGTC 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 267
PspCI CACGTG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 2 cut(s) 91, 265
PsuI RGATCY 1 cut(s) 348
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SaqAI TTAA 2 cut(s) 294, 358
SatI GCNGC 3 cut(s) 211, 214, 273
Sau3AI GATC 1 cut(s) 348
Sau96I GGNCC 2 cut(s) 91, 265
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 3 cut(s) 90, 197, 269
SduI GDGCHC 1 cut(s) 207
SinI GGWCC 1 cut(s) 265
Sse9I AATT 1 cut(s) 342
SsiI CCGC 2 cut(s) 97, 157
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 3 cut(s) 88, 195, 267
StyI CCWWGG 2 cut(s) 75, 309
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 4 cut(s) 72, 168, 191, 300
TaqI TCGA 1 cut(s) 258
TaqII GACCGA 1 cut(s) 197
TasI AATT 1 cut(s) 342
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 2 cut(s) 294, 358
Tru9I TTAA 2 cut(s) 294, 358
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 3 cut(s) 210, 213, 272
Tsp45I GTSAC 1 cut(s) 298
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 98
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.