RchiOBHm_Chr2g0158341

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0020321
Physical Location & Seq
Reverse (-)
74667710 .. 74669155
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52703

Sequence Viewer

Length: 1446 bp
ATGGGAGGGATTGGAAAGACAACTCTTTCTCAACTAGTGTATAATGATAGAAAAGTGAGAGCTCATTTTGATAAGAGGATATGGATATGCGTCTCAGACCCTTTTGAAGAGATCCAAATTCTTGCGGCTATCGCCCAAGGTCTTGATATTCCATATCACCCCAAAGCAAATGCGTTGGAAACTTATTCGCAATTTATATATGACAGCATTAAGGATGTAGAAAAAAAGAAAAGAAAAATTAAGAAAAAAGAAAAAACAAAATTTCTCATAGTCCTAGATGATGTATGGAATCCAACTGCATATCAATGGGAACAAGTAATCAAACCTTTGAGCGTGGGTGCTAAAGGGAGTAAAATATTGGTGACCACCCGAATTGAGAAGGTTGCAGTTATGATGGGGGTAGCCAATAACGTCATCCATTTGGAAATGTTGAGTGAAGAATATTGCCGATTGTTGTTCTTTCACTTTGCATCCATGGGTAGTAGGGAAAGAAAGGAGACTAGAATGCTTGAAGTTGGAAATGAACTTGTAAAGAAGTGTAAGGGTTTGCCACTTGCTGCGAAGACTTTGGGTAGTCTCATGCGCGGTAAGAAAACATTGGAAGAATGGGAAGGTGTTCTGAATAGTAGGATATGGAAATTAAAAGAGTTTGAGGAAAATGTCTTTCAGCCGCTATTACTAAGTTATTATGATCTGGCTCCGACCATTAGAAGATGTCTTTTATATTGTGCTATTTTTCCGAAAGACTATCAAATGGATAGAGTTGATTTGATTCATCTATGGATGTCACAAGATTATCTTAATGTCAAGGAAAAAACAGAAGACATTTATATGATAGGGGAAGCATATTTTGAAGACTTGGTGATGCGGTCTTTCTTCCAAGATTTTAAGCGAGATGTTGAAGGAAATATTAGTTGCTTCAAGATGCATGATATTGTTCATGATTTTGTACAGTATCTAACAAATAATGAAATTTCGATTCTCGATGTCAGGTATCCTAACAGGAGATTAGAGCTACCAAATGCCAAGGTTCATCATGTAAACTTAACATTTGACAATGAGAGTCTACTTTTTCCTCCTGCAAATTTGCGTACGGTCATATTGGATGGCTACTGGGGATATGGTGCAGAGTCTCTTTTTCAATTGAAAAGTCTTAGGACGTTGAAAATGAGTAAATTTTGGGGTATTATTCCGAATGAGCTGGGGGGATTGATACATCTGAGATATCTCAACTTAAGTAACAATCATTCGGAGGAATTACCCGAGAGTATGTGTGAACTGTACAATCTGCAAACCTTGATAGTTGCCGAAAGCAGTGCACTTCAAAGACTACCCCAGGGGGTGGGGAAGCTTATCAACTTAAGGCATCTTGATGTCAGGGGATGTATCCGTCTGAGATTAACAGGGATTGGGAAATTAGCTGGTCTGCGGACGGTACTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

481

Amino Acids

55.71

Weight (kDa)

8.84

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 159 3.9e-27 NB-ARC domain
WHD_DRP PF23559 245 - 317 4.1e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 379 - 477 2.2e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26300
rosa_chinensis RchiOBHm_Chr2g0158341
rosa_laevigata RLG00000021078
rosa_rugosa Rorug02G0467400
rosa_wichuraiana Rw2G044170 Rw5G026050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1342
AccI GTMKAC 1 cut(s) 1066
AccII CGCG 1 cut(s) 585
AciI CCGC 5 cut(s) 125, 585, 671, 868, 1429
AclWI GGATC 1 cut(s) 106
AcsI RAATTY 5 cut(s) 117, 260, 972, 1084, 1175
AfaI GTAC 4 cut(s) 951, 1093, 1283, 1437
AfiI CCNNNNNNNGG 1 cut(s) 1342
AflII CTTAAG 2 cut(s) 1234, 1360
AgsI TTSAA 9 cut(s) 107, 512, 854, 902, 922, 1142, 1147, 1165, 1325
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 1 cut(s) 1335
AluBI AGCT 5 cut(s) 62, 1015, 1201, 1351, 1421
AluI AGCT 5 cut(s) 62, 1015, 1201, 1351, 1421
Alw21I GWGCWC 2 cut(s) 64, 1321
Alw26I GTCTC 4 cut(s) 97, 491, 581, 1137
Alw44I GTGCAC 1 cut(s) 1317
AlwI GGATC 1 cut(s) 106
Ama87I CYCGRG 1 cut(s) 1262
ApaLI GTGCAC 1 cut(s) 1317
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 5 cut(s) 117, 260, 972, 1084, 1175
Asp700I GAANNNNTTC 1 cut(s) 615
AspLEI GCGC 1 cut(s) 585
AsuHPI GGTGA 3 cut(s) 149, 373, 874
AvaI CYCGRG 1 cut(s) 1262
BaeGI GKGCMC 1 cut(s) 1321
BanII GRGCYC 1 cut(s) 64
BbsI GAAGAC 3 cut(s) 569, 828, 861
Bbv12I GWGCWC 2 cut(s) 64, 1321
BbvI GCAGC 1 cut(s) 544
BccI CCATC 2 cut(s) 388, 1100
BcgI CGANNNNNNTGC 2 cut(s) 1298, 1332
BciT130I CCWGG 1 cut(s) 1337
BciVI GTATCC 2 cut(s) 1005, 1397
BcoDI GTCTC 4 cut(s) 97, 491, 581, 1137
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 3 cut(s) 35, 275, 501
BfrI CTTAAG 2 cut(s) 1234, 1360
BfuI GTATCC 2 cut(s) 1005, 1397
BisI GCNGC 3 cut(s) 126, 558, 671
BlsI GCNGC 3 cut(s) 127, 559, 672
Bme1390I CCNGG 1 cut(s) 1337
BmeT110I CYCGRG 1 cut(s) 1262
BmiI GGNNCC 1 cut(s) 699
BmrFI CCNGG 1 cut(s) 1337
BmrI ACTGGG 1 cut(s) 1123
BmsI GCATC 4 cut(s) 479, 855, 915, 1375
BmuI ACTGGG 1 cut(s) 1123
BpiI GAAGAC 3 cut(s) 569, 828, 861
BsaJI CCNNGG 5 cut(s) 136, 474, 1026, 1335, 1336
Bsc4I CCNNNNNNNGG 1 cut(s) 1342
Bse1I ACTGG 1 cut(s) 1118
BseBI CCWGG 1 cut(s) 1337
BseDI CCNNGG 5 cut(s) 136, 474, 1026, 1335, 1336
BseGI GGATG 6 cut(s) 220, 414, 470, 789, 1111, 1388
BseLI CCNNNNNNNGG 1 cut(s) 1342
BseMII CTCAG 3 cut(s) 108, 1211, 1385
BseNI ACTGG 1 cut(s) 1118
BseSI GKGCMC 1 cut(s) 1321
BseXI GCAGC 1 cut(s) 544
BseYI CCCAGC 1 cut(s) 1201
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 64, 1321
BsiHKCI CYCGRG 1 cut(s) 1262
BsiWI CGTACG 1 cut(s) 1091
BslI CCNNNNNNNGG 1 cut(s) 1342
BsmAI GTCTC 4 cut(s) 97, 491, 581, 1137
BsmBI CGTCTC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 510
BsoBI CYCGRG 1 cut(s) 1262
Bsp1286I GDGCHC 2 cut(s) 64, 1321
Bsp1407I TGTACA 2 cut(s) 949, 1281
Bsp143I GATC 2 cut(s) 111, 691
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 5 cut(s) 125, 585, 671, 868, 1429
BspCNI CTCAG 3 cut(s) 107, 1212, 1386
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 1 cut(s) 699
BspPI GGATC 1 cut(s) 106
BspTI CTTAAG 2 cut(s) 1234, 1360
BsrGI TGTACA 2 cut(s) 949, 1281
BsrI ACTGG 1 cut(s) 1118
BssECI CCNNGG 5 cut(s) 136, 474, 1026, 1335, 1336
BssMI GATC 2 cut(s) 111, 691
BssT1I CCWWGG 3 cut(s) 136, 474, 1026
Bst2UI CCWGG 1 cut(s) 1337
Bst4CI ACNGT 4 cut(s) 954, 1096, 1281, 1435
Bst6I CTCTTC 1 cut(s) 102
BstAFI CTTAAG 2 cut(s) 1234, 1360
BstAUI TGTACA 2 cut(s) 949, 1281
BstDEI CTNAG 6 cut(s) 94, 680, 1154, 1220, 1394, 1439
BstDSI CCRYGG 1 cut(s) 474
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 6 cut(s) 220, 414, 470, 789, 1111, 1388
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 1 cut(s) 585
BstKTI GATC 2 cut(s) 114, 694
BstMAI GTCTC 4 cut(s) 97, 491, 581, 1137
BstMBI GATC 2 cut(s) 111, 691
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 1337
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 1335
BstSLI GKGCMC 1 cut(s) 1321
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 544
BstV2I GAAGAC 3 cut(s) 569, 828, 861
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BsuI GTATCC 2 cut(s) 1005, 1397
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 6 cut(s) 220, 414, 470, 789, 1111, 1388
BtsI GCAGTG 1 cut(s) 1321
BtsIMutI CAGTG 1 cut(s) 1321
CciI TCATGA 1 cut(s) 940
CfoI GCGC 1 cut(s) 585
CseI GACGC 1 cut(s) 79
Csp6I GTAC 4 cut(s) 950, 1092, 1282, 1436
CspCI CAANNNNNGTGG 2 cut(s) 355, 390
CviAII CATG 5 cut(s) 475, 580, 929, 941, 1037
CviQI GTAC 4 cut(s) 950, 1092, 1282, 1436
DdeI CTNAG 6 cut(s) 94, 680, 1154, 1220, 1394, 1439
DpnI GATC 2 cut(s) 113, 693
DpnII GATC 2 cut(s) 111, 691
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 62
Eco130I CCWWGG 3 cut(s) 136, 474, 1026
Eco24I GRGCYC 1 cut(s) 64
Eco32I GATATC 1 cut(s) 1226
Eco53kI GAGCTC 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 1262
Eco91I GGTNACC 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 1335
EcoRV GATATC 1 cut(s) 1226
EcoT14I CCWWGG 3 cut(s) 136, 474, 1026
EcoT22I ATGCAT 1 cut(s) 930
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 3 cut(s) 136, 474, 1026
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 5 cut(s) 478, 583, 932, 944, 1040
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FatI CATG 5 cut(s) 474, 579, 928, 940, 1036
FblI GTMKAC 1 cut(s) 1066
Fnu4HI GCNGC 3 cut(s) 126, 558, 671
FokI GGATG 6 cut(s) 227, 401, 457, 796, 1118, 1395
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 3 cut(s) 126, 558, 671
FspBI CTAG 3 cut(s) 35, 275, 501
GlaI GCGC 1 cut(s) 584
GluI GCNGC 3 cut(s) 126, 558, 671
GsaI CCCAGC 1 cut(s) 1205
HgaI GACGC 1 cut(s) 79
HhaI GCGC 1 cut(s) 585
Hin1II CATG 5 cut(s) 478, 583, 932, 944, 1040
Hin6I GCGC 1 cut(s) 583
HinP1I GCGC 1 cut(s) 583
HindIII AAGCTT 1 cut(s) 1349
HinfI GANTC 5 cut(s) 289, 772, 979, 1063, 1130
HphI GGTGA 3 cut(s) 149, 373, 874
Hpy166II GTNNAC 4 cut(s) 1042, 1067, 1277, 1319
Hpy188I TCNGA 8 cut(s) 97, 621, 702, 741, 1194, 1221, 1252, 1395
Hpy188III TCNNGA 5 cut(s) 143, 922, 941, 983, 1370
Hpy8I GTNNAC 4 cut(s) 1042, 1067, 1277, 1319
HpyAV CCTTC 3 cut(s) 373, 605, 896
HpyCH4III ACNGT 4 cut(s) 954, 1096, 1281, 1435
HpyCH4IV ACGT 2 cut(s) 411, 1160
HpyCH4V TGCA 8 cut(s) 299, 386, 470, 928, 1082, 1127, 1291, 1319
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 6 cut(s) 94, 680, 1154, 1220, 1394, 1439
HpySE526I ACGT 2 cut(s) 411, 1160
Hsp92II CATG 5 cut(s) 478, 583, 932, 944, 1040
HspAI GCGC 1 cut(s) 583
Kzo9I GATC 2 cut(s) 111, 691
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 4 cut(s) 479, 855, 915, 1375
MaeI CTAG 3 cut(s) 35, 275, 501
MaeII ACGT 2 cut(s) 411, 1160
MaeIII GTNAC 3 cut(s) 361, 786, 1238
MalI GATC 2 cut(s) 113, 693
MboI GATC 2 cut(s) 111, 691
MboII GAAGA 8 cut(s) 119, 449, 574, 614, 723, 833, 866, 868
MfeI CAATTG 1 cut(s) 1142
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 2 cut(s) 64, 1321
MlyI GAGTC 2 cut(s) 1072, 1139
MmeI TCCRAC 4 cut(s) 156, 317, 496, 725
MnlI CCTC 4 cut(s) 69, 646, 1086, 1246
Mph1103I ATGCAT 1 cut(s) 930
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 9 cut(s) 210, 240, 641, 801, 888, 1046, 1235, 1361, 1400
MslI CAYNNNNRTG 3 cut(s) 304, 830, 1371
MspCI CTTAAG 2 cut(s) 1234, 1360
MspR9I CCNGG 1 cut(s) 1337
MunI CAATTG 1 cut(s) 1142
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 1 cut(s) 1337
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 1 cut(s) 131
NcoI CCATGG 1 cut(s) 474
NdeII GATC 2 cut(s) 111, 691
NlaIII CATG 5 cut(s) 478, 583, 932, 944, 1040
NlaIV GGNNCC 1 cut(s) 699
NmuCI GTSAC 2 cut(s) 361, 786
NsiI ATGCAT 1 cut(s) 930
PagI TCATGA 1 cut(s) 940
PasI CCCWGGG 1 cut(s) 1336
PctI GAATGC 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 3 cut(s) 289, 772, 979
Pfl23II CGTACG 1 cut(s) 1091
PflMI CCANNNNNTGG 1 cut(s) 1342
PkrI GCNGC 3 cut(s) 127, 559, 672
PleI GAGTC 2 cut(s) 1071, 1138
PpsI GAGTC 2 cut(s) 1071, 1138
Psp124BI GAGCTC 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 1335
PspEI GGTNACC 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 1 cut(s) 1335
PspLI CGTACG 1 cut(s) 1091
PspN4I GGNNCC 1 cut(s) 699
PsuI RGATCY 1 cut(s) 111
RsaI GTAC 4 cut(s) 951, 1093, 1283, 1437
RsaNI GTAC 4 cut(s) 950, 1092, 1282, 1436
RseI CAYNNNNRTG 3 cut(s) 304, 830, 1371
SacI GAGCTC 1 cut(s) 64
SaqAI TTAA 9 cut(s) 210, 240, 641, 801, 888, 1046, 1235, 1361, 1400
SatI GCNGC 3 cut(s) 126, 558, 671
Sau3AI GATC 2 cut(s) 111, 691
SchI GAGTC 2 cut(s) 1072, 1139
ScrFI CCNGG 1 cut(s) 1337
SduI GDGCHC 2 cut(s) 64, 1321
SfaNI GCATC 4 cut(s) 479, 855, 915, 1375
SmiMI CAYNNNNRTG 3 cut(s) 304, 830, 1371
SmlI CTYRAG 2 cut(s) 1234, 1360
SmoI CTYRAG 2 cut(s) 1234, 1360
SpeI ACTAGT 1 cut(s) 34
SsiI CCGC 5 cut(s) 125, 585, 671, 868, 1429
SspI AATATT 3 cut(s) 357, 443, 910
SspMI CTAG 3 cut(s) 35, 275, 501
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 1 cut(s) 1335
StyI CCWWGG 3 cut(s) 136, 474, 1026
TaaI ACNGT 4 cut(s) 954, 1096, 1281, 1435
TaiI ACGT 2 cut(s) 414, 1163
TaqI TCGA 2 cut(s) 977, 984
TatI WGTACW 2 cut(s) 949, 1281
TauI GCSGC 2 cut(s) 128, 673
TfiI GAWTC 3 cut(s) 289, 772, 979
Tru1I TTAA 9 cut(s) 210, 240, 641, 801, 888, 1046, 1235, 1361, 1400
Tru9I TTAA 9 cut(s) 210, 240, 641, 801, 888, 1046, 1235, 1361, 1400
TscAI CASTG 1 cut(s) 1321
TseFI GTSAC 2 cut(s) 361, 786
TseI GCWGC 1 cut(s) 557
Tsp45I GTSAC 2 cut(s) 361, 786
TspDTI ATGAA 5 cut(s) 537, 764, 929, 984, 1022
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 1 cut(s) 1321
Van91I CCANNNNNTGG 1 cut(s) 1342
Vha464I CTTAAG 2 cut(s) 1234, 1360
VneI GTGCAC 1 cut(s) 1317
XapI RAATTY 5 cut(s) 117, 260, 972, 1084, 1175
XmiI GTMKAC 1 cut(s) 1066
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 3 cut(s) 35, 275, 501
Zsp2I ATGCAT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.