RchiOBHm_Chr3g0476701

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0029915
Physical Location & Seq
Forward (+)
22685207 .. 22686242
1036 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44209

Sequence Viewer

Length: 462 bp
ATGCTTTTTCGTACTAGTAAAGACTTGGTCCCAAACGAGCTTGAGGACACTAGGAGATACCCGGATCTGCAATTTTTCCATCTCAGCTCAATAACTCTCCGGTCAATGAGCTTGGCCAAGGCAGTTTGGCTCCGTCTATCTGTATTGAATCATATTCAGGGTCAGTTACCAAAGGTGCAGAAGATTGCTGTAAAAAGGTTGTCTAAGACTTCTGGACAAGAAATTGAAGAATTCAAAAATGAAGTTGGGCTGATAGCCAGACTTCAACACAGGAATCTTGTGAAACTTATAGGCTGTTGTATACATGGAGAAGAAAGGATATTAGTACTCGAAAACATGCCTAACAAGGGCTTGGATTCATTTATTTTTGATTCAACAAGAAGATCCTTTTTGGACTGGCGAAAGCGCTTTGAAATTATCAATGGAGTTGCTCGTGGGGTTTTGTATCTTCATCAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

153

Amino Acids

17.83

Weight (kDa)

9.74

Isoelectric Point (pI)

60.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 58 - 152 1.7e-14 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 59 - 152 1.1e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029915)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0476701
rosa_roxburghii Rroxscaffold_6G00405060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AclWI GGATC 2 cut(s) 72, 378
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 230
AfaI GTAC 2 cut(s) 13, 327
AfeI AGCGCT 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 6 cut(s) 148, 227, 235, 266, 375, 413
AhlI ACTAGT 1 cut(s) 14
AluBI AGCT 3 cut(s) 40, 87, 111
AluI AGCT 3 cut(s) 40, 87, 111
AlwI GGATC 2 cut(s) 72, 378
Aor51HI AGCGCT 1 cut(s) 407
AoxI GGCC 1 cut(s) 114
ApoI RAATTY 1 cut(s) 230
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 62
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 116
BauI CACGAG 1 cut(s) 432
BccI CCATC 1 cut(s) 87
BcnI CCSGG 1 cut(s) 62
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 2 cut(s) 15, 51
BfoI RGCGCY 1 cut(s) 409
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 2 cut(s) 30, 131
BmrFI CCNGG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 97
BseNI ACTGG 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 1 cut(s) 116
BsiSI CCGG 2 cut(s) 62, 100
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 14
BsnI GGCC 1 cut(s) 116
Bsp143I GATC 2 cut(s) 64, 383
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 30, 131
BspPI GGATC 2 cut(s) 72, 378
BsrI ACTGG 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 64, 383
BssNAI GTATAC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 117
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 432
BstDEI CTNAG 2 cut(s) 83, 204
BstH2I RGCGCY 1 cut(s) 409
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 2 cut(s) 67, 386
BstMBI GATC 2 cut(s) 64, 383
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 60
BstX2I RGATCY 2 cut(s) 64, 383
BstYI RGATCY 2 cut(s) 64, 383
BstZ17I GTATAC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 116
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 12, 326
CviAII CATG 2 cut(s) 305, 337
CviJI RGCY 9 cut(s) 40, 87, 111, 116, 130, 250, 257, 294, 351
CviKI_1 RGCY 9 cut(s) 40, 87, 111, 116, 130, 250, 257, 294, 351
CviQI GTAC 2 cut(s) 12, 326
DdeI CTNAG 2 cut(s) 83, 204
DpnI GATC 2 cut(s) 66, 385
DpnII GATC 2 cut(s) 64, 383
EaeI YGGCCR 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 28
Eco47III AGCGCT 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 2 cut(s) 308, 340
FaiI YATR 5 cut(s) 153, 290, 302, 306, 338
FaqI GGGAC 1 cut(s) 14
FatI CATG 2 cut(s) 304, 336
FblI GTMKAC 1 cut(s) 301
FspBI CTAG 2 cut(s) 15, 51
GlaI GCGC 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 409
HaeIII GGCC 1 cut(s) 116
HapII CCGG 2 cut(s) 62, 100
HhaI GCGC 1 cut(s) 408
Hin1II CATG 2 cut(s) 308, 340
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HinfI GANTC 4 cut(s) 148, 274, 356, 371
HpaII CCGG 2 cut(s) 62, 100
Hpy166II GTNNAC 1 cut(s) 302
Hpy188III TCNNGA 2 cut(s) 213, 455
Hpy8I GTNNAC 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 70, 178
HpyF3I CTNAG 2 cut(s) 83, 204
Hsp92II CATG 2 cut(s) 308, 340
HspAI GCGC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 64, 383
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 7 cut(s) 75, 113, 143, 198, 256, 271, 382
MaeI CTAG 2 cut(s) 15, 51
MaeIII GTNAC 1 cut(s) 165
MalI GATC 2 cut(s) 66, 385
MboI GATC 2 cut(s) 64, 383
MboII GAAGA 5 cut(s) 193, 239, 323, 393, 440
MflI RGATCY 2 cut(s) 64, 383
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 4 cut(s) 71, 222, 230, 414
MluNI TGGCCA 1 cut(s) 116
MnlI CCTC 1 cut(s) 37
Mox20I TGGCCA 1 cut(s) 116
MscI TGGCCA 1 cut(s) 116
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 2 cut(s) 62, 100
MspR9I CCNGG 1 cut(s) 62
NciI CCSGG 1 cut(s) 62
NdeII GATC 2 cut(s) 64, 383
NlaIII CATG 2 cut(s) 308, 340
NlaIV GGNNCC 2 cut(s) 30, 131
NspI RCATGY 1 cut(s) 340
PfeI GAWTC 4 cut(s) 148, 274, 356, 371
PflFI GACNNNGTC 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 30, 131
PspPI GGNCC 1 cut(s) 28
PsuI RGATCY 2 cut(s) 64, 383
PsyI GACNNNGTC 1 cut(s) 26
RsaI GTAC 2 cut(s) 13, 327
RsaNI GTAC 2 cut(s) 12, 326
Sau3AI GATC 2 cut(s) 64, 383
Sau96I GGNCC 1 cut(s) 28
ScaI AGTACT 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 62
SetI ASST 5 cut(s) 42, 89, 113, 177, 200
SinI GGWCC 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 4 cut(s) 71, 222, 230, 414
SspMI CTAG 2 cut(s) 15, 51
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 117
TaqI TCGA 1 cut(s) 330
TasI AATT 4 cut(s) 71, 222, 230, 414
TatI WGTACW 1 cut(s) 325
TfiI GAWTC 4 cut(s) 148, 274, 356, 371
TspDTI ATGAA 3 cut(s) 255, 348, 440
TspGWI ACGGA 1 cut(s) 122
Tth111I GACNNNGTC 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 1 cut(s) 230
XceI RCATGY 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 301
XspI CTAG 2 cut(s) 15, 51
ZrmI AGTACT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.