RchiOBHm_Chr4g0403121

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0024476
Physical Location & Seq
Forward (+)
22166021 .. 22166638
618 bp
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UTR
Exon/CDS
Intron
PRQ37488

Sequence Viewer

Length: 618 bp
ATGCACATTGCTACTCTCACCATGGGAAACCCACAGATACTCTTACTCGTACCATATGGACTAGTTGTGTTACTTCACTTCTCATGCTTCCTGAAGTTAACCTCATCTTCCACTTCCAACTTCACAGATCAAACTGCTCTTCTTGCCATTCAATCCAAACTCACATTTGATCCTACTGAAACTGTCTTGGGTGGCAACTGGACCACCAACACAAGCTTCTGTGATTGGATTGGGGTCTCTTGCGGTAATCGAAGGCAAAGAGTCATCGCCTTGGACCTGTCATTTATGGGCCTCCAAGGCACAATTGCACCACACGTTGGCAACCTCTCCTTTCTGGTCTCATTGGATCTCCGAAATAACAGCTTCTCTGGGTTCCTGCCGAATGAAATCAGCCGTTTACATTGCTTGAAGGTACTCATGCTGTATGCCAACCAGTTGGAGGGAAACATCCCTCCAACTTTGTATCGCTGTGAAAAGCTTGAAATTATTAGCCTTGCAATAAACAAGCTTAGCGGCCATATACCTGAAGAGTTGGCTTTCTTACCCAGACTTGAGCAACTATTTCTTGGTCACAATAATTTTTCTGGTGGAGTTCTGTTACGATCCCCAGGATCGTAA

Protein Analysis

205

Amino Acids

22.56

Weight (kDa)

7.0

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 81 7.7e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 96 - 187 1.6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024476)

Species Orthologous Gene IDs
malus_domestica MD07G1168800.v1.1
rosa_chinensis RchiOBHm_Chr4g0403121 RchiOBHm_Chr4g0403151

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 317
AciI CCGC 2 cut(s) 243, 513
AclWI GGATC 3 cut(s) 164, 354, 597
AcoI YGGCCR 1 cut(s) 514
AcuI CTGAAG 2 cut(s) 113, 546
AfaI GTAC 2 cut(s) 51, 414
AfiI CCNNNNNNNGG 2 cut(s) 317, 439
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 3 cut(s) 152, 409, 482
AhlI ACTAGT 1 cut(s) 61
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 4 cut(s) 216, 363, 478, 508
AluI AGCT 4 cut(s) 216, 363, 478, 508
Alw26I GTCTC 2 cut(s) 241, 343
AlwI GGATC 3 cut(s) 164, 354, 597
AoxI GGCC 2 cut(s) 289, 514
AspS9I GGNCC 3 cut(s) 201, 274, 289
AsuHPI GGTGA 1 cut(s) 10
AvaII GGWCC 2 cut(s) 201, 274
BceAI ACGGC 1 cut(s) 378
BciT130I CCWGG 1 cut(s) 609
BcoDI GTCTC 2 cut(s) 241, 343
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 1 cut(s) 62
BglI GCCNNNNNGGC 1 cut(s) 297
BisI GCNGC 1 cut(s) 514
BlpI GCTNAGC 1 cut(s) 509
BlsI GCNGC 1 cut(s) 515
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 2 cut(s) 201, 274
BmgT120I GGNCC 3 cut(s) 201, 274, 289
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 609
Bpu1102I GCTNAGC 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 572
BsaI GGTCTC 2 cut(s) 241, 343
BsaJI CCNNGG 4 cut(s) 21, 270, 295, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 439
Bse1I ACTGG 2 cut(s) 203, 433
Bse3DI GCAATG 2 cut(s) 6, 400
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 4 cut(s) 21, 270, 295, 607
BseGI GGATG 1 cut(s) 447
BseLI CCNNNNNNNGG 2 cut(s) 317, 439
BseMI GCAATG 2 cut(s) 6, 400
BseNI ACTGG 2 cut(s) 203, 433
BshFI GGCC 2 cut(s) 291, 516
BslI CCNNNNNNNGG 2 cut(s) 317, 439
BsmAI GTCTC 2 cut(s) 241, 343
BsnI GGCC 2 cut(s) 291, 516
Bso31I GGTCTC 2 cut(s) 241, 343
Bsp143I GATC 5 cut(s) 127, 169, 346, 602, 611
Bsp1720I GCTNAGC 1 cut(s) 509
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 2 cut(s) 243, 513
BspANI GGCC 2 cut(s) 291, 516
BspLI GGNNCC 1 cut(s) 374
BspPI GGATC 3 cut(s) 164, 354, 597
BspQI GCTCTTC 1 cut(s) 144
BspTNI GGTCTC 2 cut(s) 241, 343
BsrDI GCAATG 2 cut(s) 6, 400
BsrI ACTGG 2 cut(s) 203, 433
BssECI CCNNGG 4 cut(s) 21, 270, 295, 607
BssMI GATC 5 cut(s) 127, 169, 346, 602, 611
BssT1I CCWWGG 3 cut(s) 21, 270, 295
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 2 cut(s) 144, 522
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 447
BstKTI GATC 5 cut(s) 130, 172, 349, 605, 614
BstMAI GTCTC 2 cut(s) 241, 343
BstMBI GATC 5 cut(s) 127, 169, 346, 602, 611
BstMWI GCNNNNNNNGC 2 cut(s) 143, 297
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 607
BstX2I RGATCY 1 cut(s) 346
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 1 cut(s) 346
BsuRI GGCC 2 cut(s) 291, 516
BtgI CCRYGG 1 cut(s) 21
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 447
Cfr13I GGNCC 3 cut(s) 201, 274, 289
Csp6I GTAC 2 cut(s) 50, 413
CviAII CATG 3 cut(s) 22, 84, 418
CviJI RGCY 9 cut(s) 216, 291, 363, 393, 478, 492, 508, 516, 536
CviKI_1 RGCY 9 cut(s) 216, 291, 363, 393, 478, 492, 508, 516, 536
CviQI GTAC 2 cut(s) 50, 413
DdeI CTNAG 1 cut(s) 509
DpnI GATC 5 cut(s) 129, 171, 348, 604, 613
DpnII GATC 5 cut(s) 127, 169, 346, 602, 611
EaeI YGGCCR 1 cut(s) 514
Eam1104I CTCTTC 2 cut(s) 144, 522
EarI CTCTTC 2 cut(s) 144, 522
Eco130I CCWWGG 3 cut(s) 21, 270, 295
Eco31I GGTCTC 2 cut(s) 241, 343
Eco47I GGWCC 2 cut(s) 201, 274
Eco57I CTGAAG 2 cut(s) 113, 546
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 3 cut(s) 21, 270, 295
ErhI CCWWGG 3 cut(s) 21, 270, 295
FaeI CATG 3 cut(s) 25, 87, 421
FaiI YATR 9 cut(s) 23, 55, 57, 85, 287, 419, 426, 519, 521
FatI CATG 3 cut(s) 21, 83, 417
FauNDI CATATG 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 514
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 1 cut(s) 514
FspBI CTAG 1 cut(s) 62
GluI GCNGC 1 cut(s) 514
HaeIII GGCC 2 cut(s) 291, 516
Hin1II CATG 3 cut(s) 25, 87, 421
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 3 cut(s) 214, 476, 506
HinfI GANTC 1 cut(s) 261
HpaI GTTAAC 1 cut(s) 99
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 2 cut(s) 99, 398
Hpy188I TCNGA 1 cut(s) 353
Hpy188III TCNNGA 1 cut(s) 91
Hpy8I GTNNAC 2 cut(s) 99, 398
HpyAV CCTTC 2 cut(s) 246, 403
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 3 cut(s) 4, 308, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 297
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 3 cut(s) 25, 87, 421
KspAI GTTAAC 1 cut(s) 99
Kzo9I GATC 5 cut(s) 127, 169, 346, 602, 611
LguI GCTCTTC 1 cut(s) 144
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 3 cut(s) 69, 569, 597
MalI GATC 5 cut(s) 129, 171, 348, 604, 613
MboI GATC 5 cut(s) 127, 169, 346, 602, 611
MboII GAAGA 3 cut(s) 99, 131, 539
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 346
MluCI AATT 3 cut(s) 303, 483, 577
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 3 cut(s) 141, 417, 479
MnlI CCTC 5 cut(s) 112, 302, 335, 433, 462
MseI TTAA 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 609
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 609
MwoI GCNNNNNNNGC 2 cut(s) 143, 297
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 55
NdeII GATC 5 cut(s) 127, 169, 346, 602, 611
NlaIII CATG 3 cut(s) 25, 87, 421
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 1 cut(s) 515
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 3 cut(s) 201, 274, 289
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 51, 414
RsaNI GTAC 2 cut(s) 50, 413
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 1 cut(s) 514
Sau3AI GATC 5 cut(s) 127, 169, 346, 602, 611
Sau96I GGNCC 3 cut(s) 201, 274, 289
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 609
SinI GGWCC 2 cut(s) 201, 274
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SpeI ACTAGT 1 cut(s) 61
Sse9I AATT 3 cut(s) 303, 483, 577
SsiI CCGC 2 cut(s) 243, 513
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 3 cut(s) 21, 270, 295
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 318
TaqI TCGA 1 cut(s) 250
TasI AATT 3 cut(s) 303, 483, 577
TauI GCSGC 1 cut(s) 516
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TseFI GTSAC 1 cut(s) 569
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 1 cut(s) 399
Van91I CCANNNNNTGG 1 cut(s) 317
VpaK11BI GGWCC 2 cut(s) 201, 274
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.