RchiOBHm_Chr5g0007011

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0030223
Physical Location & Seq
Reverse (-)
4347150 .. 4347573
424 bp
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UTR
Exon/CDS
Intron
PRQ28811

Sequence Viewer

Length: 255 bp
ATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGATGGGTAGTTTCTGGTGGGTTTGACAATGTTCTAAAGGTGTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAACGACATATTCGGTCTATAGATTTTCACCCCCTTGAGTTTCTCTTGGCCACAGGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

84

Amino Acids

9.74

Weight (kDa)

8.86

Isoelectric Point (pI)

9.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Aladin PF25460 1 - 54 7.9e-07 Aladin seven-bladed propeller
WD40_WDHD1_1st PF24817 2 - 68 1e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 2 - 83 3e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 2 - 67 2.6e-06 TEP-1 second beta-propeller
EIF3I PF24805 2 - 75 3.9e-08 EIF3I
WD40_Gbeta PF25391 2 - 83 2.2e-10 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 2 - 83 2.3e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 2 - 82 1.6e-13 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 2 - 82 9.9e-15 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 2 - 82 1e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 2 - 83 4.6e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 2 - 82 3.2e-19 WDR5 beta-propeller domain
WDR55 PF24796 4 - 57 2.4e-07 WDR55
Beta-prop_WDR3_2nd PF25172 4 - 75 3.2e-08 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 4 - 50 3.2e-06 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 4 - 77 6.4e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_1st PF24780 8 - 72 8.5e-06 MABP1/WDR62 first WD40 domain
Beta-prop_SPT8 PF23798 13 - 77 1.8e-06 SPT8 beta propeller
WD40 PF00400 15 - 51 5.1e-13 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 16 - 82 1.3e-07 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0030223)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0007011
rosa_multiflora Rmu_sc0010774.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 1 cut(s) 82
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
AlwI GGATC 1 cut(s) 159
AoxI GGCC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 212
BalI TGGCCA 1 cut(s) 242
BccI CCATC 2 cut(s) 95, 102
BfmI CTRYAG 1 cut(s) 210
BglII AGATCT 1 cut(s) 18
BmcAI AGTACT 1 cut(s) 82
BmsI GCATC 2 cut(s) 32, 54
BpuEI CTTGAG 2 cut(s) 54, 248
BseGI GGATG 2 cut(s) 45, 47
BseYI CCCAGC 1 cut(s) 161
BshFI GGCC 1 cut(s) 242
BslFI GGGAC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 1 cut(s) 242
Bsp143I GATC 2 cut(s) 18, 151
BspANI GGCC 1 cut(s) 242
BspPI GGATC 1 cut(s) 159
BssMI GATC 2 cut(s) 18, 151
BstF5I GGATG 2 cut(s) 45, 47
BstKTI GATC 2 cut(s) 21, 154
BstMBI GATC 2 cut(s) 18, 151
BstSFI CTRYAG 1 cut(s) 210
BstX2I RGATCY 2 cut(s) 18, 151
BstYI RGATCY 2 cut(s) 18, 151
BsuRI GGCC 1 cut(s) 242
BtsCI GGATG 2 cut(s) 45, 47
Csp6I GTAC 1 cut(s) 81
CviAII CATG 2 cut(s) 176, 191
CviJI RGCY 2 cut(s) 169, 242
CviKI_1 RGCY 2 cut(s) 169, 242
CviQI GTAC 1 cut(s) 81
DpnI GATC 2 cut(s) 20, 153
DpnII GATC 2 cut(s) 18, 151
DraI TTTAAA 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 35
EcoT22I ATGCAT 1 cut(s) 47
FaeI CATG 2 cut(s) 179, 194
FaiI YATR 6 cut(s) 55, 66, 177, 192, 201, 212
FaqI GGGAC 1 cut(s) 38
FatI CATG 2 cut(s) 175, 190
FokI GGATG 2 cut(s) 32, 54
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 242
Hin1II CATG 2 cut(s) 179, 194
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 31
HpyCH4V TGCA 2 cut(s) 45, 175
Hsp92II CATG 2 cut(s) 179, 194
Kzo9I GATC 2 cut(s) 18, 151
LpnPI CCDG 4 cut(s) 7, 105, 147, 231
LweI GCATC 2 cut(s) 32, 54
MalI GATC 2 cut(s) 20, 153
MboI GATC 2 cut(s) 18, 151
MboII GAAGA 1 cut(s) 28
MflI RGATCY 2 cut(s) 18, 151
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 1 cut(s) 185
MluNI TGGCCA 1 cut(s) 242
Mox20I TGGCCA 1 cut(s) 242
Mph1103I ATGCAT 1 cut(s) 47
MscI TGGCCA 1 cut(s) 242
MseI TTAA 2 cut(s) 87, 183
Msp20I TGGCCA 1 cut(s) 242
NdeII GATC 2 cut(s) 18, 151
NlaIII CATG 2 cut(s) 179, 194
NsiI ATGCAT 1 cut(s) 47
PspFI CCCAGC 1 cut(s) 161
PsuI RGATCY 2 cut(s) 18, 151
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 87, 183
Sau3AI GATC 2 cut(s) 18, 151
ScaI AGTACT 1 cut(s) 82
SetI ASST 4 cut(s) 64, 147, 171, 250
SfaNI GCATC 2 cut(s) 32, 54
SfcI CTRYAG 1 cut(s) 210
SmlI CTYRAG 2 cut(s) 69, 227
SmoI CTYRAG 2 cut(s) 69, 227
Sse9I AATT 1 cut(s) 185
TaqI TCGA 1 cut(s) 106
TaqII GACCGA 1 cut(s) 195
TasI AATT 1 cut(s) 185
TatI WGTACW 1 cut(s) 80
Tru1I TTAA 2 cut(s) 87, 183
Tru9I TTAA 2 cut(s) 87, 183
TspDTI ATGAA 1 cut(s) 207
XapI RAATTY 1 cut(s) 185
ZrmI AGTACT 1 cut(s) 82
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.