RchiOBHm_Chr5g0017211

NADH-ubiquinone/plastoquinone oxidoreductase chain 4L

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0023867
Physical Location & Seq
Forward (+)
11957982 .. 11958287
306 bp
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UTR
Exon/CDS
Intron
PRQ29752

Sequence Viewer

Length: 306 bp
ATGATGCTCGAGCATGTACTTGTTTTGAGTGCCTACTTATTTTCTATCGGTATCTATGGATTGATCACGAGCCGAAATATGGTTAGAGCCCTTATGTGTCTTGAACTTATACTGAATGCGGTTAATATAAATTTCGTAACATTTTCTGATTTTTTTGATAGCCGGCAATTAAAAGGAAATATTTTCTCCATTTTTGTTATAGCTATTGCCGCCGCTGAAGCAGCTATTGGACCGGCTATTGTTTCGTCAATTTATCGTAACAGAAAATCAACTCGTATCAATCAATCGAATTTGTTGAATAAGTAG

Protein Analysis

101

Amino Acids

11.26

Weight (kDa)

9.74

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q2 PF00420 5 - 100 2.8e-29 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023867)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01070
rosa_chinensis RchiOBHm_Chr5g0017211 RchiOBHm_CPg0502771

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 119, 210, 213
AcsI RAATTY 2 cut(s) 130, 289
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 2 cut(s) 104, 298
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AluBI AGCT 2 cut(s) 203, 224
AluI AGCT 2 cut(s) 203, 224
Ama87I CYCGRG 1 cut(s) 8
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 2 cut(s) 130, 289
AspS9I GGNCC 1 cut(s) 230
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 91
BauI CACGAG 1 cut(s) 67
BbvI GCAGC 1 cut(s) 233
BclI TGATCA 1 cut(s) 63
BisI GCNGC 3 cut(s) 210, 213, 222
BlsI GCNGC 3 cut(s) 211, 214, 223
Bme18I GGWCC 1 cut(s) 230
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 230
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse118I RCCGGY 2 cut(s) 162, 232
BseLI CCNNNNNNNGG 1 cut(s) 79
BseXI GCAGC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 2 cut(s) 163, 233
BslI CCNNNNNNNGG 1 cut(s) 79
BsmI GAATGC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 91
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 3 cut(s) 119, 210, 213
BsrFI RCCGGY 2 cut(s) 162, 232
BssAI RCCGGY 2 cut(s) 162, 232
BssMI GATC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 66
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 209, 218, 221
BstNSI RCATGY 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 233
Cac8I GCNNGC 1 cut(s) 164
Cfr10I RCCGGY 2 cut(s) 162, 232
Cfr13I GGNCC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 17
CviAII CATG 1 cut(s) 14
CviJI RGCY 6 cut(s) 72, 89, 162, 203, 224, 236
CviKI_1 RGCY 6 cut(s) 72, 89, 162, 203, 224, 236
CviQI GTAC 1 cut(s) 17
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 8
EcoT38I GRGCYC 1 cut(s) 91
FaeI CATG 1 cut(s) 17
FaiI YATR 7 cut(s) 15, 57, 80, 95, 110, 128, 200
FatI CATG 1 cut(s) 13
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 210, 213, 222
FriOI GRGCYC 1 cut(s) 91
Fsp4HI GCNGC 3 cut(s) 210, 213, 222
GluI GCNGC 3 cut(s) 210, 213, 222
HapII CCGG 2 cut(s) 163, 233
Hin1II CATG 1 cut(s) 17
HpaII CCGG 2 cut(s) 163, 233
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 67, 101
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 218, 221
Hsp92II CATG 1 cut(s) 17
KroI GCCGGC 1 cut(s) 162
KroNI GCCGGC 1 cut(s) 164
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 1 cut(s) 63
LpnPI CCDG 2 cut(s) 176, 246
Lsp1109I GCAGC 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 136, 257
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 4 cut(s) 130, 167, 249, 289
MroNI GCCGGC 1 cut(s) 162
MseI TTAA 2 cut(s) 123, 170
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 2 cut(s) 163, 233
Mva1269I GAATGC 1 cut(s) 121
MwoI GCNNNNNNNGC 3 cut(s) 209, 218, 221
NaeI GCCGGC 1 cut(s) 164
NdeII GATC 1 cut(s) 63
NgoMIV GCCGGC 1 cut(s) 162
NlaIII CATG 1 cut(s) 17
NspI RCATGY 1 cut(s) 17
PaeR7I CTCGAG 1 cut(s) 8
PctI GAATGC 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 164
PkrI GCNGC 3 cut(s) 211, 214, 223
PspPI GGNCC 1 cut(s) 230
PspXI VCTCGAGB 1 cut(s) 8
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 123, 170
SatI GCNGC 3 cut(s) 210, 213, 222
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 230
SduI GDGCHC 1 cut(s) 91
SetI ASST 2 cut(s) 205, 226
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 4 cut(s) 130, 167, 249, 289
SsiI CCGC 3 cut(s) 119, 210, 213
SspI AATATT 1 cut(s) 181
TaqI TCGA 2 cut(s) 9, 287
TasI AATT 4 cut(s) 130, 167, 249, 289
TatI WGTACW 1 cut(s) 16
TauI GCSGC 2 cut(s) 212, 215
Tru1I TTAA 2 cut(s) 123, 170
Tru9I TTAA 2 cut(s) 123, 170
TseI GCWGC 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 230
XapI RAATTY 2 cut(s) 130, 289
XceI RCATGY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.