RchiOBHm_Chr5g0017531

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0030143
Physical Location & Seq
Forward (+)
12157536 .. 12158110
575 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29783

Sequence Viewer

Length: 384 bp
ATGTTTTACTTTGGAACTTGTTTCTTCAACCTGATTTTGGAAAAGGTAGCTGGTTCAAGAGGCGGATCTACAGCTGTTACAGCAATACTTATCAATGGAGAGACGCTAATTGTAGCCAATGTCGGCGACTCCCGGGCAGTTTTATGCAGAGATGGTGTGGCGAACCAGTTAACTGTAGATCACGACCCGCAGAAGGAGAAGGATCTTGTTGAAAGCAGAGGTGGTTGTGTTGTTAAGCTGCCAGGAAATGTTCCACGTGTGGATGCACAACTCGCAATGTCGCATGGTAGAATCAGAACAGAGTCCATTAGCATCGAAACAGTGAGTTGGATTGATTCATTGGCGAAATTACTAAATAGTAGTCACATTTTGCAGTTCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C RVT_3

Protein Analysis

127

Amino Acids

13.7

Weight (kDa)

6.26

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 19 - 94 3.3e-16 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030143)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017531
rosa_samantha Rh5CG141200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 63, 188
AclWI GGATC 2 cut(s) 73, 210
AcvI CACGTG 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 37, 193
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 3 cut(s) 28, 57, 212
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 3 cut(s) 50, 74, 238
AluI AGCT 3 cut(s) 50, 74, 238
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 2 cut(s) 73, 210
Ama87I CYCGRG 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 238
AsuC2I CCSGG 2 cut(s) 133, 134
AvaI CYCGRG 1 cut(s) 132
BbrPI CACGTG 1 cut(s) 257
BbvI GCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 2 cut(s) 133, 134
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 1 cut(s) 382
BfmI CTRYAG 2 cut(s) 69, 174
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 3 cut(s) 133, 134, 243
BmeT110I CYCGRG 1 cut(s) 132
BmrFI CCNGG 3 cut(s) 133, 134, 243
BmsI GCATC 2 cut(s) 253, 321
BpuMI CCSGG 2 cut(s) 133, 134
BsaAI YACGTR 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 193
Bse1I ACTGG 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 2 cut(s) 37, 193
BseMI GCAATG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 37, 193
BsmAI GTCTC 1 cut(s) 95
BsmBI CGTCTC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 132
Bsp143I GATC 3 cut(s) 65, 178, 202
BspACI CCGC 2 cut(s) 63, 188
BspPI GGATC 2 cut(s) 73, 210
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 3 cut(s) 65, 178, 202
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 175, 322
BstBAI YACGTR 1 cut(s) 257
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 3 cut(s) 68, 181, 205
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 65, 178, 202
BstMWI GCNNNNNNNGC 2 cut(s) 80, 272
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 3 cut(s) 131, 132, 241
BstSFI CTRYAG 2 cut(s) 69, 174
BstV1I GCAGC 1 cut(s) 225
BstX2I RGATCY 2 cut(s) 65, 202
BstYI RGATCY 2 cut(s) 65, 202
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 327
Cfr9I CCCGGG 1 cut(s) 132
CseI GACGC 1 cut(s) 112
CviAII CATG 1 cut(s) 284
CviJI RGCY 4 cut(s) 50, 74, 116, 238
CviKI_1 RGCY 4 cut(s) 50, 74, 116, 238
DpnI GATC 3 cut(s) 67, 180, 204
DpnII GATC 3 cut(s) 65, 178, 202
EciI GGCGGA 1 cut(s) 78
Eco72I CACGTG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 1 cut(s) 287
FaiI YATR 2 cut(s) 145, 285
FatI CATG 1 cut(s) 283
FauI CCCGC 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 1 cut(s) 382
GluI GCNGC 1 cut(s) 239
HapII CCGG 1 cut(s) 133
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 287
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 4 cut(s) 128, 291, 302, 335
HpaI GTTAAC 1 cut(s) 171
HpaII CCGG 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 296
Hpy188III TCNNGA 2 cut(s) 57, 182
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 2 cut(s) 187, 193
HpyCH4III ACNGT 2 cut(s) 175, 322
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 3 cut(s) 147, 266, 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 272
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 1 cut(s) 287
KspAI GTTAAC 1 cut(s) 171
Kzo9I GATC 3 cut(s) 65, 178, 202
LpnPI CCDG 6 cut(s) 36, 44, 146, 179, 228, 255
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 2 cut(s) 253, 321
MaeI CTAG 1 cut(s) 382
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 2 cut(s) 76, 362
MalI GATC 3 cut(s) 67, 180, 204
MboI GATC 3 cut(s) 65, 178, 202
MboII GAAGA 1 cut(s) 16
MflI RGATCY 2 cut(s) 65, 202
MluCI AATT 2 cut(s) 108, 347
MlyI GAGTC 2 cut(s) 122, 311
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 2 cut(s) 53, 212
MseI TTAA 2 cut(s) 170, 234
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 3 cut(s) 133, 134, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 80, 272
NciI CCSGG 2 cut(s) 133, 134
NdeII GATC 3 cut(s) 65, 178, 202
NlaIII CATG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 362
PfeI GAWTC 2 cut(s) 291, 335
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 2 cut(s) 122, 310
PmaCI CACGTG 1 cut(s) 257
PmlI CACGTG 1 cut(s) 257
PpsI GAGTC 2 cut(s) 122, 310
Ppu21I YACGTR 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 241
PspCI CACGTG 1 cut(s) 257
PspGI CCWGG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 65, 202
PvuII CAGCTG 1 cut(s) 74
SaqAI TTAA 2 cut(s) 170, 234
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 3 cut(s) 65, 178, 202
SchI GAGTC 2 cut(s) 122, 311
ScrFI CCNGG 3 cut(s) 133, 134, 243
SetI ASST 7 cut(s) 33, 48, 52, 76, 223, 240, 259
SfaNI GCATC 2 cut(s) 253, 321
SfcI CTRYAG 2 cut(s) 69, 174
SmaI CCCGGG 1 cut(s) 134
Sse9I AATT 2 cut(s) 108, 347
SsiI CCGC 2 cut(s) 63, 188
SspMI CTAG 1 cut(s) 382
StyD4I CCNGG 3 cut(s) 131, 132, 241
TaaI ACNGT 2 cut(s) 175, 322
TaiI ACGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 315
TasI AATT 2 cut(s) 108, 347
TfiI GAWTC 2 cut(s) 291, 335
Tru1I TTAA 2 cut(s) 170, 234
Tru9I TTAA 2 cut(s) 170, 234
TscAI CASTG 1 cut(s) 327
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 327
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 1 cut(s) 327
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.