Rh5CG141200

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0030143
Physical Location & Seq
Forward (+)
12328318 .. 12342616
14299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG141200.1

Sequence Viewer

Length: 531 bp
ATGTTTTACTTTGGAACTTGTTTCTTCAACCTGATTTTGGAAAAGGTAGCTGGTTCAAGAGGCGGATCTACAGCTGTTACAGCAATACTTATCAATGGAGAGACGCTAATTGTAGCCAATGTCGGCGACTCCCGGGCAGTTTTATGCAGAGATGGTGTGGCGAACCAGTTAACTGTAGATCACGACCCGCAGAAGGAGAAGGATCTTGTTGAAAGCAGAGGTGGTTGTGTTGTTAAGCTGCCAGGCGTTAAAGTTGCCAACACAAAGCTAGCTAGATCTCGGAGAAGCATTGTGCCTAAAATCAGAAGGCATTTTGAAGTGGACGACACCACTTTGATGAGACACATGCAATTCTTTGCAAATTTTACACATCAATCCATTTATAGATGTTTCTTCATTGTTGTTCAGCACAACACATTTCAGCTGTCTTTCAGTCCTTTATACAAAGCCTCAGGTTCAACAACACAGCAAATCGAAAGCCTCGAGTCATCTTTGCCTTGGTGTAGGCTCAGTGGAACATCTCAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C RVT_3

Protein Analysis

176

Amino Acids

19.66

Weight (kDa)

9.43

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 19 - 94 2.3e-15 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030143)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017531
rosa_samantha Rh5CG141200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 63, 188, 526
AclWI GGATC 2 cut(s) 73, 210
AcsI RAATTY 1 cut(s) 361
AfiI CCNNNNNNNGG 2 cut(s) 37, 193
AgsI TTSAA 5 cut(s) 28, 57, 212, 317, 459
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 6 cut(s) 50, 74, 238, 268, 272, 424
AluI AGCT 6 cut(s) 50, 74, 238, 268, 272, 424
Alw26I GTCTC 2 cut(s) 95, 334
AlwI GGATC 2 cut(s) 73, 210
Ama87I CYCGRG 2 cut(s) 132, 482
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 361
AsuC2I CCSGG 2 cut(s) 133, 134
AsuNHI GCTAGC 1 cut(s) 268
AvaI CYCGRG 2 cut(s) 132, 482
AxyI CCTNAGG 1 cut(s) 451
BbvI GCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 2 cut(s) 133, 134
BcoDI GTCTC 2 cut(s) 95, 334
BfaI CTAG 2 cut(s) 269, 273
BfmI CTRYAG 2 cut(s) 69, 174
BglII AGATCT 1 cut(s) 275
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 3 cut(s) 133, 134, 243
BmeT110I CYCGRG 2 cut(s) 132, 482
BmrFI CCNGG 3 cut(s) 133, 134, 243
BmtI GCTAGC 1 cut(s) 272
BpuMI CCSGG 2 cut(s) 133, 134
BsaJI CCNNGG 2 cut(s) 132, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 193
Bse1I ACTGG 1 cut(s) 166
Bse21I CCTNAGG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 132, 497
BseLI CCNNNNNNNGG 2 cut(s) 37, 193
BseMII CTCAG 2 cut(s) 465, 523
BseNI ACTGG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 225
BsiHKCI CYCGRG 2 cut(s) 132, 482
BsiSI CCGG 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 37, 193
BsmAI GTCTC 2 cut(s) 95, 334
BsmBI CGTCTC 1 cut(s) 95
BsoBI CYCGRG 2 cut(s) 132, 482
Bsp143I GATC 4 cut(s) 65, 178, 202, 275
BspACI CCGC 3 cut(s) 63, 188, 526
BspCNI CTCAG 2 cut(s) 464, 522
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 2 cut(s) 73, 210
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 132, 497
BssMI GATC 4 cut(s) 65, 178, 202, 275
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 3 cut(s) 451, 509, 522
BstKTI GATC 4 cut(s) 68, 181, 205, 278
BstMAI GTCTC 2 cut(s) 95, 334
BstMBI GATC 4 cut(s) 65, 178, 202, 275
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 243
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 3 cut(s) 131, 132, 241
BstSFI CTRYAG 2 cut(s) 69, 174
BstV1I GCAGC 1 cut(s) 225
BstX2I RGATCY 3 cut(s) 65, 202, 275
BstYI RGATCY 3 cut(s) 65, 202, 275
Bsu36I CCTNAGG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 517
Cac8I GCNNGC 1 cut(s) 270
Cfr9I CCCGGG 1 cut(s) 132
CseI GACGC 1 cut(s) 112
CviAII CATG 1 cut(s) 346
DdeI CTNAG 3 cut(s) 451, 509, 522
DpnI GATC 4 cut(s) 67, 180, 204, 277
DpnII GATC 4 cut(s) 65, 178, 202, 275
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 451
Eco88I CYCGRG 2 cut(s) 132, 482
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 497
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 1 cut(s) 349
FaiI YATR 4 cut(s) 145, 347, 384, 442
FatI CATG 1 cut(s) 345
FauI CCCGC 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 2 cut(s) 269, 273
GluI GCNGC 1 cut(s) 239
HapII CCGG 1 cut(s) 133
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 2 cut(s) 128, 485
HpaI GTTAAC 1 cut(s) 171
HpaII CCGG 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 171, 322
Hpy188I TCNGA 2 cut(s) 282, 305
Hpy188III TCNNGA 2 cut(s) 57, 182
Hpy8I GTNNAC 2 cut(s) 171, 322
HpyAV CCTTC 3 cut(s) 187, 193, 300
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4V TGCA 3 cut(s) 147, 349, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 3 cut(s) 451, 509, 522
Hsp92II CATG 1 cut(s) 349
KspAI GTTAAC 1 cut(s) 171
Kzo9I GATC 4 cut(s) 65, 178, 202, 275
LpnPI CCDG 7 cut(s) 36, 44, 146, 179, 228, 255, 438
Lsp1109I GCAGC 1 cut(s) 225
MaeI CTAG 2 cut(s) 269, 273
MaeIII GTNAC 1 cut(s) 76
MalI GATC 4 cut(s) 67, 180, 204, 277
MboI GATC 4 cut(s) 65, 178, 202, 275
MboII GAAGA 2 cut(s) 16, 385
MflI RGATCY 3 cut(s) 65, 202, 275
MluCI AATT 3 cut(s) 108, 350, 361
MlyI GAGTC 2 cut(s) 122, 494
MnlI CCTC 4 cut(s) 53, 212, 460, 491
MseI TTAA 3 cut(s) 170, 234, 249
MslI CAYNNNNRTG 1 cut(s) 335
MspA1I CMGCKG 3 cut(s) 74, 424, 526
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 3 cut(s) 133, 134, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 80
NciI CCSGG 2 cut(s) 133, 134
NdeII GATC 4 cut(s) 65, 178, 202, 275
NheI GCTAGC 1 cut(s) 268
NlaIII CATG 1 cut(s) 349
NspI RCATGY 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 2 cut(s) 122, 493
PpsI GAGTC 2 cut(s) 122, 493
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspXI VCTCGAGB 1 cut(s) 482
PsuI RGATCY 3 cut(s) 65, 202, 275
PvuII CAGCTG 2 cut(s) 74, 424
RseI CAYNNNNRTG 1 cut(s) 335
SaqAI TTAA 3 cut(s) 170, 234, 249
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 4 cut(s) 65, 178, 202, 275
SchI GAGTC 2 cut(s) 122, 494
ScrFI CCNGG 3 cut(s) 133, 134, 243
SfcI CTRYAG 2 cut(s) 69, 174
Sfr274I CTCGAG 1 cut(s) 482
SlaI CTCGAG 1 cut(s) 482
SmaI CCCGGG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 3 cut(s) 108, 350, 361
SsiI CCGC 3 cut(s) 63, 188, 526
SspMI CTAG 2 cut(s) 269, 273
StyD4I CCNGG 3 cut(s) 131, 132, 241
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 474, 483
TasI AATT 3 cut(s) 108, 350, 361
Tru1I TTAA 3 cut(s) 170, 234, 249
Tru9I TTAA 3 cut(s) 170, 234, 249
TscAI CASTG 1 cut(s) 517
TseI GCWGC 1 cut(s) 238
TspDTI ATGAA 1 cut(s) 385
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 1 cut(s) 517
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 482
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 2 cut(s) 269, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.