RchiOBHm_Chr5g0027101

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0024998
Physical Location & Seq
Forward (+)
21049521 .. 21050972
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30665

Sequence Viewer

Length: 612 bp
ATGAGACGCCTTGTGAAGATTATGGTATTAAAAAGGCCAGACATTGTAAAAACGACTGATGCAATTGGATGGACTCCGTTGCACTACGCAGCATTGAGAGGGTACCTTAACGTAACTAGAGTATTGCTGAATTGCGATAGTTCTACACTTTATATTTTGGATAAAACTGGAATGTCGGCTCTCCATGTCGCGGCTTGTGCAGGTCACACTAAAGTATTGAAAGAGTTCATTCGACGTCGGCCTGATGCTTGTGATTTGCTCAATGATAAAGGCCAAAACATTCTTCACTATGCAATTTTAGGTGAATGTATATTTGTCGTCAAGTACATATTGAAGACCGCTAAACTTGCCAGGCTCATAAATGAAGCAGATAATGATGGAAACACTCCTCTGCATCTAGCTGCCATTTACAGAATAGAGGACTCTATTATCGGAAGTTTCGCATCCGATCCTAGAGTTGATAGGACTGCTATTAATAACGAGTTCTTACATGCCGTCGACATCTATGTTCGCCGCAATTTTAAACTGGTACGTGTCCATACGAAAGATAAGATCTCTCTATCTCCAACTAGATCACATTTGTCCATCATCTTATTAATTTTGTGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

203

Amino Acids

23.0

Weight (kDa)

9.6

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 4 - 77 1.3e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 72 - 140 2.6e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024998)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0027101
rosa_rugosa Rorug05G0097400
rosa_samantha Rh5DG190400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 191
Acc65I GGTACC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 498
AccII CGCG 1 cut(s) 191
AciI CCGC 3 cut(s) 191, 339, 514
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 605
AcyI GRCGYC 2 cut(s) 7, 235
AfaI GTAC 3 cut(s) 104, 326, 531
AfiI CCNNNNNNNGG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 2 cut(s) 220, 334
AjnI CCWGG 1 cut(s) 350
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
AluBI AGCT 1 cut(s) 401
AluI AGCT 1 cut(s) 401
AlwI GGATC 1 cut(s) 443
AoxI GGCC 4 cut(s) 35, 239, 271, 605
ApeKI GCWGC 2 cut(s) 89, 401
AseI ATTAAT 2 cut(s) 474, 596
Asp700I GAANNNNTTC 1 cut(s) 224
Asp718I GGTACC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 314
BalI TGGCCA 1 cut(s) 607
BanI GGYRCC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 341
BbvI GCAGC 2 cut(s) 101, 388
BccI CCATC 3 cut(s) 63, 371, 593
BceAI ACGGC 1 cut(s) 479
BciT130I CCWGG 1 cut(s) 352
BfaI CTAG 4 cut(s) 117, 398, 453, 570
BfuAI ACCTGC 1 cut(s) 191
BglII AGATCT 1 cut(s) 552
BisI GCNGC 4 cut(s) 90, 192, 402, 514
BlsI GCNGC 4 cut(s) 91, 193, 403, 515
Bme1390I CCNGG 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 352
BmsI GCATC 4 cut(s) 49, 235, 403, 452
BpiI GAAGAC 1 cut(s) 341
BsaAI YACGTR 1 cut(s) 533
BsaHI GRCGYC 2 cut(s) 7, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 2 cut(s) 172, 531
BseBI CCWGG 1 cut(s) 352
BseGI GGATG 2 cut(s) 74, 443
BseLI CCNNNNNNNGG 1 cut(s) 190
BseNI ACTGG 2 cut(s) 172, 531
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 2 cut(s) 101, 388
BsgI GTGCAG 1 cut(s) 219
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 4 cut(s) 37, 241, 273, 607
BshNI GGYRCC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 190
BsnI GGCC 4 cut(s) 37, 241, 273, 607
Bsp143I GATC 3 cut(s) 448, 552, 572
BspACI CCGC 3 cut(s) 191, 339, 514
BspANI GGCC 4 cut(s) 37, 241, 273, 607
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 104
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 102
BsrI ACTGG 2 cut(s) 172, 531
BssMI GATC 3 cut(s) 448, 552, 572
BssNI GRCGYC 2 cut(s) 7, 235
Bst2UI CCWGG 1 cut(s) 352
BstACI GRCGYC 2 cut(s) 7, 235
BstBAI YACGTR 1 cut(s) 533
BstF5I GGATG 2 cut(s) 74, 443
BstFNI CGCG 1 cut(s) 191
BstKTI GATC 3 cut(s) 451, 555, 575
BstMBI GATC 3 cut(s) 448, 552, 572
BstMWI GCNNNNNNNGC 2 cut(s) 197, 347
BstNI CCWGG 1 cut(s) 352
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 350
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 101, 388
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 552
BstYI RGATCY 1 cut(s) 552
BsuRI GGCC 4 cut(s) 37, 241, 273, 607
BtsCI GGATG 2 cut(s) 74, 443
BveI ACCTGC 1 cut(s) 191
CseI GACGC 1 cut(s) 15
Csp6I GTAC 3 cut(s) 103, 325, 530
CviAII CATG 2 cut(s) 185, 491
CviJI RGCY 8 cut(s) 37, 179, 194, 241, 273, 355, 401, 607
CviKI_1 RGCY 8 cut(s) 37, 179, 194, 241, 273, 355, 401, 607
CviQI GTAC 3 cut(s) 103, 325, 530
DpnI GATC 3 cut(s) 450, 554, 574
DpnII GATC 3 cut(s) 448, 552, 572
DraI TTTAAA 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 350
FaeI CATG 2 cut(s) 188, 494
FatI CATG 2 cut(s) 184, 490
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 4 cut(s) 90, 192, 402, 514
FokI GGATG 2 cut(s) 81, 430
Fsp4HI GCNGC 4 cut(s) 90, 192, 402, 514
FspBI CTAG 4 cut(s) 117, 398, 453, 570
GluI GCNGC 4 cut(s) 90, 192, 402, 514
HaeIII GGCC 4 cut(s) 37, 241, 273, 607
HgaI GACGC 1 cut(s) 15
Hin1I GRCGYC 2 cut(s) 7, 235
Hin1II CATG 2 cut(s) 188, 494
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 2 cut(s) 73, 422
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 2 cut(s) 434, 448
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 3 cut(s) 237, 240, 500
HpyCH4IV ACGT 3 cut(s) 111, 235, 532
HpyCH4V TGCA 5 cut(s) 62, 82, 200, 293, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 347
HpySE526I ACGT 3 cut(s) 111, 235, 532
Hsp92I GRCGYC 2 cut(s) 7, 235
Hsp92II CATG 2 cut(s) 188, 494
KpnI GGTACC 1 cut(s) 106
Kzo9I GATC 3 cut(s) 448, 552, 572
LpnPI CCDG 7 cut(s) 51, 153, 186, 255, 337, 364, 512
Lsp1109I GCAGC 2 cut(s) 101, 388
LweI GCATC 4 cut(s) 49, 235, 403, 452
MaeI CTAG 4 cut(s) 117, 398, 453, 570
MaeII ACGT 3 cut(s) 111, 235, 532
MaeIII GTNAC 2 cut(s) 112, 203
MalI GATC 3 cut(s) 450, 554, 574
MboI GATC 3 cut(s) 448, 552, 572
MboII GAAGA 3 cut(s) 28, 275, 346
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 1 cut(s) 552
MlsI TGGCCA 1 cut(s) 607
MluCI AATT 5 cut(s) 63, 130, 294, 517, 597
MluNI TGGCCA 1 cut(s) 607
MlyI GAGTC 2 cut(s) 67, 416
MmeI TCCRAC 1 cut(s) 590
MnlI CCTC 3 cut(s) 92, 399, 412
Mox20I TGGCCA 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 1 cut(s) 607
MseI TTAA 5 cut(s) 29, 108, 474, 522, 596
Msp20I TGGCCA 1 cut(s) 607
MspR9I CCNGG 1 cut(s) 352
MunI CAATTG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 352
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 197, 347
NdeII GATC 3 cut(s) 448, 552, 572
NlaIII CATG 2 cut(s) 188, 494
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 203
NspI RCATGY 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 224
PkrI GCNGC 4 cut(s) 91, 193, 403, 515
PleI GAGTC 2 cut(s) 67, 416
PpsI GAGTC 2 cut(s) 67, 416
Ppu21I YACGTR 1 cut(s) 533
PshBI ATTAAT 2 cut(s) 474, 596
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 1 cut(s) 104
PsuI RGATCY 1 cut(s) 552
RsaI GTAC 3 cut(s) 104, 326, 531
RsaNI GTAC 3 cut(s) 103, 325, 530
SalI GTCGAC 1 cut(s) 497
SaqAI TTAA 5 cut(s) 29, 108, 474, 522, 596
SatI GCNGC 4 cut(s) 90, 192, 402, 514
Sau3AI GATC 3 cut(s) 448, 552, 572
SchI GAGTC 2 cut(s) 67, 416
ScrFI CCNGG 1 cut(s) 352
SetI ASST 7 cut(s) 108, 114, 205, 238, 304, 403, 535
SfaNI GCATC 4 cut(s) 49, 235, 403, 452
Sse9I AATT 5 cut(s) 63, 130, 294, 517, 597
SsiI CCGC 3 cut(s) 191, 339, 514
SspMI CTAG 4 cut(s) 117, 398, 453, 570
StyD4I CCNGG 1 cut(s) 350
TaiI ACGT 3 cut(s) 114, 238, 535
TaqI TCGA 2 cut(s) 232, 498
TasI AATT 5 cut(s) 63, 130, 294, 517, 597
TatI WGTACW 1 cut(s) 324
TauI GCSGC 2 cut(s) 194, 516
Tru1I TTAA 5 cut(s) 29, 108, 474, 522, 596
Tru9I TTAA 5 cut(s) 29, 108, 474, 522, 596
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 2 cut(s) 89, 401
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 2 cut(s) 217, 378
TspGWI ACGGA 1 cut(s) 66
VspI ATTAAT 2 cut(s) 474, 596
XceI RCATGY 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 498
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 4 cut(s) 117, 398, 453, 570
ZraI GACGTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.