RchiOBHm_Chr5g0058631

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0030178
Physical Location & Seq
Reverse (-)
63126048 .. 63130882
4835 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33526

Sequence Viewer

Length: 1032 bp
ATGCTTCCTCCTTCACAACCAAAATTCGGAGATATCTATGTTGTGTTTGAGTTGATGGAATCATACCTTCAGCATGTTATCAAGGCAAATGATGATCTTACCCCTGAGCATTATCAGTTTTTCCTATACCAGCTTCTAAGTGTTTTAAAGTATATTCATACAGGTTGGATTATGTTGCAACTCGATGGTACAGAGCCCCTGAACTTTGTGGCTGATTTTTCTCAAAATTATCGATCTAAGCATGCTTATGAATTAGGTGTCACTTTGTTTGAGTTTCTATCGGAGGGACTTGGCCTGAAACCCGACCATCTTATAGGCTTGCATTGTGTAAAGGGACAATTTATTGTTGGTAATTACTATCCACCATGCCCTGAGCCTGAATTTACTCTTGGTGTTGGCAAACACACAGATAATACTTTTTTCACCATGCTACTTGAAGACAACGTCAACGCGCTCCAGGTTTTGTATCAAAACCAGTGGATTGATGTCCCTTCCTATGCCCGGAGTTCTTGTGGGTTTTGCTATGGTTTGCTGGGGTCGATTTGTAGGGTCAAGAGAGGGAAAGATCATATGTCTGCCAATGAATTGAGGAAGAAACCAAGGGAGAGAGCAGAGTATCAAAAGAAAGTTTGCTGCTGCCAGCAAACTGCCAAAGCTGCAAAGCTGGTAGCAAATAGGTCTGCCCCATCCACATCAAGGCCTGCACAATCTACAACCAGACCTACAAGCTCCAGGCCAGCTCAAACTACATCTGCCCCAACCAGACCTACGAGTTCAAGGCTTGCACAACCTTTAATAGCATCAAGCAAGCAGGCTACAACTTCAACAAGGACACCTCAGAGGATCAGAGAAAGGTCAGTTACAAGAGATGGACTGTGTACTGGAGACCCTCGATCTGAGCCGATCAAGGAGTTGACTTCAGCAGAAAATCCCCCAATTTACCGTGGAACAACTTTGGCAGATTACATAAAGGGCCACTACATAAAAGGGTTGATCAATGGCGTGTGTGGACTAGAATATTTGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

343

Amino Acids

38.54

Weight (kDa)

8.97

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 113 - 165 2.1e-13 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030178)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0058631
rosa_samantha Rh5BG394400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 452
AclWI GGATC 1 cut(s) 851
AcsI RAATTY 2 cut(s) 23, 380
AcuI CTGAAG 2 cut(s) 53, 903
AfaI GTAC 2 cut(s) 190, 880
AfiI CCNNNNNNNGG 3 cut(s) 26, 501, 696
AgsI TTSAA 4 cut(s) 437, 777, 825, 1024
AjnI CCWGG 2 cut(s) 456, 731
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 6 cut(s) 133, 656, 664, 729, 740, 1027
AluI AGCT 6 cut(s) 133, 656, 664, 729, 740, 1027
Alw26I GTCTC 1 cut(s) 879
AlwI GGATC 1 cut(s) 851
AoxI GGCC 4 cut(s) 292, 698, 734, 973
ApeKI GCWGC 3 cut(s) 633, 636, 656
ApoI RAATTY 2 cut(s) 23, 380
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 973
AsuC2I CCSGG 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 415
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 444
BbvI GCAGC 3 cut(s) 620, 623, 643
BccI CCATC 5 cut(s) 49, 179, 315, 694, 863
BciT130I CCWGG 2 cut(s) 458, 733
BclI TGATCA 1 cut(s) 993
BcnI CCSGG 1 cut(s) 502
BcoDI GTCTC 1 cut(s) 879
BfaI CTAG 1 cut(s) 1013
BisI GCNGC 3 cut(s) 634, 637, 657
BlsI GCNGC 3 cut(s) 635, 638, 658
Bme1390I CCNGG 3 cut(s) 458, 502, 733
BmgT120I GGNCC 1 cut(s) 973
BmrFI CCNGG 3 cut(s) 458, 502, 733
BmsI GCATC 1 cut(s) 809
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 3 cut(s) 440, 715, 903
Bpu10I CCTNAGC 2 cut(s) 105, 372
BpuMI CCSGG 1 cut(s) 502
Bsa29I ATCGAT 1 cut(s) 232
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 2 cut(s) 599, 943
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 501, 696
Bse1I ACTGG 2 cut(s) 475, 886
BseBI CCWGG 2 cut(s) 458, 733
BseCI ATCGAT 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 599, 943
BseGI GGATG 1 cut(s) 686
BseLI CCNNNNNNNGG 3 cut(s) 26, 501, 696
BseMII CTCAG 4 cut(s) 96, 363, 851, 888
BseNI ACTGG 2 cut(s) 475, 886
BseXI GCAGC 3 cut(s) 620, 623, 643
BseYI CCCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 1 cut(s) 452
BshFI GGCC 4 cut(s) 294, 700, 736, 975
BshVI ATCGAT 1 cut(s) 232
BsiSI CCGG 1 cut(s) 502
BslFI GGGAC 3 cut(s) 300, 348, 473
BslI CCNNNNNNNGG 3 cut(s) 26, 501, 696
BsmAI GTCTC 1 cut(s) 879
BsmFI GGGAC 3 cut(s) 300, 348, 473
BsnI GGCC 4 cut(s) 294, 700, 736, 975
Bso31I GGTCTC 1 cut(s) 879
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
BspANI GGCC 4 cut(s) 294, 700, 736, 975
BspCNI CTCAG 4 cut(s) 97, 364, 850, 889
BspDI ATCGAT 1 cut(s) 232
BspFNI CGCG 1 cut(s) 452
BspPI GGATC 1 cut(s) 851
BspTNI GGTCTC 1 cut(s) 879
BsrI ACTGG 2 cut(s) 475, 886
BssECI CCNNGG 2 cut(s) 599, 943
BssMI GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
BssT1I CCWWGG 1 cut(s) 599
Bst2UI CCWGG 2 cut(s) 458, 733
Bst4CI ACNGT 2 cut(s) 876, 944
BstC8I GCNNGC 8 cut(s) 243, 320, 641, 702, 738, 783, 809, 813
BstDEI CTNAG 6 cut(s) 105, 137, 237, 372, 837, 897
BstDSI CCRYGG 1 cut(s) 943
BstF5I GGATG 1 cut(s) 686
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 7 cut(s) 97, 236, 568, 846, 896, 906, 996
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
BstMWI GCNNNNNNNGC 1 cut(s) 656
BstNI CCWGG 2 cut(s) 458, 733
BstNSI RCATGY 2 cut(s) 77, 245
BstSCI CCNGG 3 cut(s) 456, 500, 731
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 3 cut(s) 620, 623, 643
BstV2I GAAGAC 1 cut(s) 444
Bsu15I ATCGAT 1 cut(s) 232
BsuRI GGCC 4 cut(s) 294, 700, 736, 975
BsuTUI ATCGAT 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 943
BtsCI GGATG 1 cut(s) 686
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 8 cut(s) 243, 320, 641, 702, 738, 783, 809, 813
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 973
ClaI ATCGAT 1 cut(s) 232
Csp6I GTAC 2 cut(s) 189, 879
CspCI CAANNNNNGTGG 2 cut(s) 458, 493
CviAII CATG 4 cut(s) 74, 242, 366, 427
CviQI GTAC 2 cut(s) 189, 879
DdeI CTNAG 6 cut(s) 105, 137, 237, 372, 837, 897
DpnI GATC 7 cut(s) 96, 235, 567, 845, 895, 905, 995
DpnII GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
DraI TTTAAA 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 599
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 879
Eco32I GATATC 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 53, 903
EcoRII CCWGG 2 cut(s) 456, 731
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 599
FaeI CATG 4 cut(s) 77, 245, 369, 430
FaqI GGGAC 3 cut(s) 300, 348, 473
FatI CATG 4 cut(s) 73, 241, 365, 426
FauNDI CATATG 1 cut(s) 570
FbaI TGATCA 1 cut(s) 993
Fnu4HI GCNGC 3 cut(s) 634, 637, 657
FokI GGATG 1 cut(s) 673
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 3 cut(s) 634, 637, 657
FspBI CTAG 1 cut(s) 1013
GlaI GCGC 1 cut(s) 453
GluI GCNGC 3 cut(s) 634, 637, 657
GsaI CCCAGC 1 cut(s) 536
GsuI CTGGAG 3 cut(s) 440, 715, 903
HaeIII GGCC 4 cut(s) 294, 700, 736, 975
HapII CCGG 1 cut(s) 502
HhaI GCGC 1 cut(s) 454
Hin1II CATG 4 cut(s) 77, 245, 369, 430
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HincII GTYRAC 2 cut(s) 448, 915
HindII GTYRAC 2 cut(s) 448, 915
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 502
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 4 cut(s) 448, 879, 915, 1010
Hpy188I TCNGA 5 cut(s) 29, 283, 840, 848, 898
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 4 cut(s) 448, 879, 915, 1010
HpyAV CCTTC 3 cut(s) 21, 77, 501
HpyCH4III ACNGT 2 cut(s) 876, 944
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 5 cut(s) 178, 322, 659, 704, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 656
HpyF3I CTNAG 6 cut(s) 105, 137, 237, 372, 837, 897
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 4 cut(s) 77, 245, 369, 430
HspAI GCGC 1 cut(s) 452
Ksp22I TGATCA 1 cut(s) 993
Kzo9I GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
LmnI GCTCC 2 cut(s) 459, 734
Lsp1109I GCAGC 3 cut(s) 620, 623, 643
LweI GCATC 1 cut(s) 809
MaeI CTAG 1 cut(s) 1013
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 259, 859
MalI GATC 7 cut(s) 96, 235, 567, 845, 895, 905, 995
MboI GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
MboII GAAGA 2 cut(s) 449, 604
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 8 cut(s) 23, 226, 251, 338, 352, 380, 584, 936
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 7 cut(s) 18, 277, 551, 582, 834, 846, 900
MseI TTAA 2 cut(s) 146, 794
MslI CAYNNNNRTG 1 cut(s) 246
MspI CCGG 1 cut(s) 502
MspR9I CCNGG 3 cut(s) 458, 502, 733
MvaI CCWGG 2 cut(s) 458, 733
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 1 cut(s) 656
NciI CCSGG 1 cut(s) 502
NdeI CATATG 1 cut(s) 570
NdeII GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
NlaIII CATG 4 cut(s) 77, 245, 369, 430
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 2 cut(s) 77, 245
PaeI GCATGC 1 cut(s) 245
PceI AGGCCT 1 cut(s) 700
PfeI GAWTC 1 cut(s) 59
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 3 cut(s) 635, 638, 658
Psp6I CCWGG 2 cut(s) 456, 731
PspFI CCCAGC 1 cut(s) 532
PspGI CCWGG 2 cut(s) 456, 731
PspPI GGNCC 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 443
RsaI GTAC 2 cut(s) 190, 880
RsaNI GTAC 2 cut(s) 189, 879
RseI CAYNNNNRTG 1 cut(s) 246
SaqAI TTAA 2 cut(s) 146, 794
SatI GCNGC 3 cut(s) 634, 637, 657
Sau3AI GATC 7 cut(s) 94, 233, 565, 843, 893, 903, 993
Sau96I GGNCC 1 cut(s) 973
ScrFI CCNGG 3 cut(s) 458, 502, 733
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 1 cut(s) 809
SmiMI CAYNNNNRTG 1 cut(s) 246
SphI GCATGC 1 cut(s) 245
Sse9I AATT 8 cut(s) 23, 226, 251, 338, 352, 380, 584, 936
SseBI AGGCCT 1 cut(s) 700
SspI AATATT 1 cut(s) 1019
SspMI CTAG 1 cut(s) 1013
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 3 cut(s) 456, 500, 731
StyI CCWWGG 1 cut(s) 599
TaaI ACNGT 2 cut(s) 876, 944
TaiI ACGT 1 cut(s) 447
TaqI TCGA 4 cut(s) 183, 232, 539, 892
TasI AATT 8 cut(s) 23, 226, 251, 338, 352, 380, 584, 936
TatI WGTACW 1 cut(s) 878
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 146, 794
Tru9I TTAA 2 cut(s) 146, 794
TscAI CASTG 1 cut(s) 482
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 3 cut(s) 633, 636, 656
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 146, 264, 597
TspRI CASTG 1 cut(s) 482
Tth111I GACNNNGTC 1 cut(s) 443
XapI RAATTY 2 cut(s) 23, 380
XceI RCATGY 2 cut(s) 77, 245
XspI CTAG 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.