Rh5BG394400

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0030178
Physical Location & Seq
Reverse (-)
63472345 .. 63476020
3676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG394400.1

Sequence Viewer

Length: 453 bp
ATGCTTCCTCCTTCACAACCAAAATTCGGAGATATCTATGTTGTGTTTGAGTTGATGGAATCATACCTTCAGCATGTTATCAAGGCAAATGATGATCTTACCCCTGAGCATTATCAGTTTTTCCTATACCAGCTTCTAAGTGTTTTAAAGTATATTCATACAGGTGTCACTTTGTTTGAGTTTCTATCGGAGGGACTTGGCCTGAAACCCGACCATCTTATAGGCTTGCATTGTGTAAAGGGACAATTTATTGTTGGTAATTACTATCCACCATGCCCTGAGCCTGAATTTACTCTTGGTGTTGGCAAACACACAGATAATACTTTTTTCACCATGCTACTTGAAGACAACGTCAACGCGCTCCAGGTCAAGAGAGGGAAAGATCATATGTCTGCCAATGAATTGAGGAAGAAACCAAGGGAGAGAGCAGAGTATCAAAAGTTATTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

150

Amino Acids

17.36

Weight (kDa)

6.14

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 82 - 134 1.4e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030178)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0058631
rosa_samantha Rh5BG394400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 359
AcsI RAATTY 2 cut(s) 23, 287
AcuI CTGAAG 1 cut(s) 53
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 344
AjnI CCWGG 1 cut(s) 363
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
AoxI GGCC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 23, 287
AspLEI GCGC 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 351
BccI CCATC 2 cut(s) 49, 222
BciT130I CCWGG 1 cut(s) 365
Bme1390I CCNGG 1 cut(s) 365
BmrFI CCNGG 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 347
Bpu10I CCTNAGC 2 cut(s) 105, 279
BsaJI CCNNGG 1 cut(s) 416
Bsc4I CCNNNNNNNGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 416
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 2 cut(s) 96, 270
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 1 cut(s) 201
BslFI GGGAC 2 cut(s) 207, 255
BslI CCNNNNNNNGG 1 cut(s) 26
BsmFI GGGAC 2 cut(s) 207, 255
BsnI GGCC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 94, 382
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 2 cut(s) 97, 271
BspFNI CGCG 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 416
BssMI GATC 2 cut(s) 94, 382
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 105, 137, 279
BstFNI CGCG 1 cut(s) 359
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 2 cut(s) 97, 385
BstMBI GATC 2 cut(s) 94, 382
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 363
BstUI CGCG 1 cut(s) 359
BstV2I GAAGAC 1 cut(s) 351
BsuRI GGCC 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 227
CfoI GCGC 1 cut(s) 361
CviAII CATG 3 cut(s) 74, 273, 334
CviJI RGCY 4 cut(s) 133, 201, 225, 283
CviKI_1 RGCY 4 cut(s) 133, 201, 225, 283
DdeI CTNAG 3 cut(s) 105, 137, 279
DpnI GATC 2 cut(s) 96, 384
DpnII GATC 2 cut(s) 94, 382
DraI TTTAAA 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 416
Eco32I GATATC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 363
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 416
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 3 cut(s) 77, 276, 337
FaqI GGGAC 2 cut(s) 207, 255
FatI CATG 3 cut(s) 73, 272, 333
FauNDI CATATG 1 cut(s) 387
GlaI GCGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 1 cut(s) 201
HhaI GCGC 1 cut(s) 361
Hin1II CATG 3 cut(s) 77, 276, 337
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 1 cut(s) 59
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 2 cut(s) 29, 190
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 21, 77
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 1 cut(s) 229
HpyF3I CTNAG 3 cut(s) 105, 137, 279
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 3 cut(s) 77, 276, 337
HspAI GCGC 1 cut(s) 359
Kzo9I GATC 2 cut(s) 94, 382
LmnI GCTCC 1 cut(s) 366
LpnPI CCDG 8 cut(s) 117, 143, 147, 215, 291, 297, 350, 377
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 166
MalI GATC 2 cut(s) 96, 384
MboI GATC 2 cut(s) 94, 382
MboII GAAGA 2 cut(s) 356, 421
MluCI AATT 5 cut(s) 23, 245, 259, 287, 401
MnlI CCTC 4 cut(s) 18, 184, 368, 399
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 359
NdeI CATATG 1 cut(s) 387
NdeII GATC 2 cut(s) 94, 382
NlaIII CATG 3 cut(s) 77, 276, 337
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 77
PfeI GAWTC 1 cut(s) 59
PflFI GACNNNGTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PsyI GACNNNGTC 1 cut(s) 350
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 1 cut(s) 146
Sau3AI GATC 2 cut(s) 94, 382
ScrFI CCNGG 1 cut(s) 365
SetI ASST 5 cut(s) 69, 135, 166, 354, 369
SmiMI CAYNNNNRTG 1 cut(s) 162
Sse9I AATT 5 cut(s) 23, 245, 259, 287, 401
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 1 cut(s) 416
TaiI ACGT 1 cut(s) 354
TasI AATT 5 cut(s) 23, 245, 259, 287, 401
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TseFI GTSAC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 146, 414
Tth111I GACNNNGTC 1 cut(s) 350
XapI RAATTY 2 cut(s) 23, 287
XceI RCATGY 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.