RchiOBHm_Chr6g0246581

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0030331
Physical Location & Seq
Forward (+)
2246334 .. 2249036
2703 bp
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UTR
Exon/CDS
Intron
PRQ22103

Sequence Viewer

Length: 297 bp
ATGAAAAGGTGGAGGAGATCCAGCCTGGCACGAGCGGCCAAACCTGACCGTCAAGGTCGTCACCACCAATCCGGTCGAGAGGTCACCTCCAAGAATGGCGGCGCTCGATCCTCTCTCTCCTCTGGCCCTCTTCGACCCCCTCACATCACTGAGTGCCTCGTTAGCGATGAGACTGGAACCATTGTCTTCACTGCTCGTAATGACCAAGTTGACATTATGAAGCCTGGTACCACTGTGATCCCAAACAATGCAAAGATTGACATGTTCGATCAAGGGGACTATGAGGCTTGCAGTTGA

Protein Analysis

98

Amino Acids

10.8

Weight (kDa)

9.5

Isoelectric Point (pI)

61.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 27 - 90 5.8e-21 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030331)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0246581
rosa_multiflora Rmu_sc0005119.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 35
AciI CCGC 2 cut(s) 35, 99
AclWI GGATC 3 cut(s) 12, 102, 232
AcoI YGGCCR 1 cut(s) 36
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 1 cut(s) 229
AflIII ACRYGT 1 cut(s) 261
AjnI CCWGG 2 cut(s) 24, 223
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 3 cut(s) 12, 102, 232
AoxI GGCC 2 cut(s) 36, 124
Asp718I GGTACC 1 cut(s) 227
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 53, 76
BaeI ACNNNNGTAYC 2 cut(s) 219, 252
BanI GGYRCC 1 cut(s) 227
BauI CACGAG 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 178
BciT130I CCWGG 2 cut(s) 26, 225
BcoDI GTCTC 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 105
BisI GCNGC 2 cut(s) 36, 100
BlsI GCNGC 2 cut(s) 37, 101
Bme1390I CCNGG 2 cut(s) 26, 225
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 178, 229
BmrFI CCNGG 2 cut(s) 26, 225
BpiI GAAGAC 1 cut(s) 178
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BsaWI WCCGGW 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 178
BseBI CCWGG 2 cut(s) 26, 225
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 2 cut(s) 28, 109
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 2 cut(s) 38, 126
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 76
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 2 cut(s) 38, 126
Bsp143I GATC 4 cut(s) 17, 107, 237, 268
BspACI CCGC 2 cut(s) 35, 99
BspANI GGCC 2 cut(s) 38, 126
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 2 cut(s) 178, 229
BspPI GGATC 3 cut(s) 12, 102, 232
BspT107I GGYRCC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 35
BsrI ACTGG 1 cut(s) 178
BssMI GATC 4 cut(s) 17, 107, 237, 268
BssSI CACGAG 1 cut(s) 30
Bst2BI CACGAG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 26, 225
Bst4CI ACNGT 2 cut(s) 50, 235
Bst6I CTCTTC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 4 cut(s) 20, 110, 240, 271
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 4 cut(s) 17, 107, 237, 268
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 2 cut(s) 35, 162
BstNI CCWGG 2 cut(s) 26, 225
BstNSI RCATGY 1 cut(s) 265
BstPI GGTNACC 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 24, 223
BstV2I GAAGAC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 2 cut(s) 38, 126
BtgZI GCGATG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 147, 189, 231
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 228
CviAII CATG 1 cut(s) 262
CviJI RGCY 5 cut(s) 24, 38, 126, 223, 287
CviKI_1 RGCY 5 cut(s) 24, 38, 126, 223, 287
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 1 cut(s) 150
DpnI GATC 4 cut(s) 19, 109, 239, 270
DpnII GATC 4 cut(s) 17, 107, 237, 268
DraIII CACNNNGTG 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 24, 223
FaeI CATG 1 cut(s) 265
FaiI YATR 3 cut(s) 218, 263, 282
FaqI GGGAC 1 cut(s) 290
FatI CATG 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 36, 100
Fsp4HI GCNGC 2 cut(s) 36, 100
GlaI GCGC 1 cut(s) 103
GluI GCNGC 2 cut(s) 36, 100
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 2 cut(s) 38, 126
HapII CCGG 1 cut(s) 72
HhaI GCGC 1 cut(s) 104
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HpaII CCGG 1 cut(s) 72
HphI GGTGA 2 cut(s) 53, 76
Hpy166II GTNNAC 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 50, 235
HpyCH4V TGCA 2 cut(s) 251, 291
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 162
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 1 cut(s) 102
KpnI GGTACC 1 cut(s) 231
Kzo9I GATC 4 cut(s) 17, 107, 237, 268
LpnPI CCDG 9 cut(s) 11, 34, 38, 57, 85, 108, 159, 210, 237
MaeIII GTNAC 2 cut(s) 59, 82
MalI GATC 4 cut(s) 19, 109, 239, 270
MbiI CCGCTC 1 cut(s) 35
MboI GATC 4 cut(s) 17, 107, 237, 268
MboII GAAGA 2 cut(s) 122, 178
MflI RGATCY 1 cut(s) 17
MnlI CCTC 9 cut(s) 6, 73, 97, 121, 130, 138, 150, 167, 277
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 26, 225
MvaI CCWGG 2 cut(s) 26, 225
MwoI GCNNNNNNNGC 2 cut(s) 35, 162
NdeII GATC 4 cut(s) 17, 107, 237, 268
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 2 cut(s) 178, 229
NmuCI GTSAC 2 cut(s) 59, 82
NspI RCATGY 1 cut(s) 265
PciI ACATGT 1 cut(s) 261
PkrI GCNGC 2 cut(s) 37, 101
PscI ACATGT 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 24, 223
PspEI GGTNACC 1 cut(s) 82
PspGI CCWGG 2 cut(s) 24, 223
PspN4I GGNNCC 2 cut(s) 178, 229
PspPI GGNCC 1 cut(s) 125
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
SatI GCNGC 2 cut(s) 36, 100
Sau3AI GATC 4 cut(s) 17, 107, 237, 268
Sau96I GGNCC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 26, 225
SetI ASST 5 cut(s) 11, 46, 58, 84, 89
SsiI CCGC 2 cut(s) 35, 99
StyD4I CCNGG 2 cut(s) 24, 223
TaaI ACNGT 2 cut(s) 50, 235
TaqI TCGA 4 cut(s) 76, 106, 133, 267
TauI GCSGC 2 cut(s) 38, 102
TscAI CASTG 3 cut(s) 154, 196, 238
TseFI GTSAC 2 cut(s) 59, 82
Tsp45I GTSAC 2 cut(s) 59, 82
TspDTI ATGAA 2 cut(s) 17, 233
TspRI CASTG 3 cut(s) 154, 196, 238
XceI RCATGY 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.