RchiOBHm_Chr6g0255131

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0030374
Physical Location & Seq
Reverse (-)
10381262 .. 10381763
502 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22885

Sequence Viewer

Length: 483 bp
ATGAACCCACCAACCCCGCCGCCAGCAAGTCGACGGAATTATAATGTCTTCCTCAGTTTCAGTGGTGAAGACACCCCAGAGAGTTTTATAGAGCATCTGTGTCGAGCTTTGGATCAGAAAGGAATATCGACATTTCGAGGTGATCAACTTGAGCCTGGGGAACCAACCCGTCCAAGTGTCTTCAAAGCAATTGAGGAATCGAGATGTGCAATTGTCATTCTTTCATCCAGCTATGCCAATTCAACTAGGTGTTTGGATGAGCTTGCAAAGGCCGCTGAATGTGTGCGAATGCCGGAGGGAATATTCCCAGTTTTCTATAATGTAGATGACGCTGACGTGCGAAATCTAAGGGGCTATTTTGGGGAAGCTTTCTCCCAGCTTGAACAACGCTTCGAAGCCGATACGGAGAAGATCGGAAAATGGAGATCTTTACTTAAACGGCTAGGCAATGTCAATGGGTGGTATCCACTGAAGTCCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

160

Amino Acids

18.13

Weight (kDa)

5.93

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 154 8.9e-36 TIR domain
TIR_2 PF13676 16 - 119 1.6e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030374)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0255131
rosa_multiflora Rmu_ssc0000213.1_g000049

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 42
AccI GTMKAC 1 cut(s) 31
AciI CCGC 3 cut(s) 17, 20, 273
AclWI GGATC 1 cut(s) 120
AgsI TTSAA 3 cut(s) 184, 243, 383
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 154, 476
AluBI AGCT 5 cut(s) 107, 231, 262, 368, 379
AluI AGCT 5 cut(s) 107, 231, 262, 368, 379
AlwI GGATC 1 cut(s) 120
AoxI GGCC 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 77, 152
AsuII TTCGAA 1 cut(s) 393
BbsI GAAGAC 3 cut(s) 40, 75, 172
BceAI ACGGC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 2 cut(s) 156, 478
BciVI GTATCC 1 cut(s) 474
BclI TGATCA 1 cut(s) 142
BfaI CTAG 2 cut(s) 246, 443
BfuI GTATCC 1 cut(s) 474
BglII AGATCT 1 cut(s) 425
BisI GCNGC 2 cut(s) 20, 273
BlsI GCNGC 2 cut(s) 21, 274
Bme1390I CCNGG 2 cut(s) 156, 478
BmgBI CACGTC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 2 cut(s) 156, 478
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 103
BmuI ACTGGG 1 cut(s) 302
BpiI GAAGAC 3 cut(s) 40, 75, 172
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 454
BseBI CCWGG 2 cut(s) 156, 478
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 2 cut(s) 224, 262
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 1 cut(s) 67
BseNI ACTGG 1 cut(s) 308
BseYI CCCAGC 1 cut(s) 375
BshFI GGCC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 293
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 393
Bsp143I GATC 4 cut(s) 112, 142, 411, 425
BspACI CCGC 3 cut(s) 17, 20, 273
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 162
BspPI GGATC 1 cut(s) 120
BspT104I TTCGAA 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 454
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 4 cut(s) 112, 142, 411, 425
Bst2UI CCWGG 2 cut(s) 156, 478
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 24, 264
BstDEI CTNAG 2 cut(s) 53, 347
BstF5I GGATG 2 cut(s) 224, 262
BstKTI GATC 4 cut(s) 115, 145, 414, 428
BstMBI GATC 4 cut(s) 112, 142, 411, 425
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 2 cut(s) 156, 478
BstSCI CCNGG 2 cut(s) 154, 476
BstV2I GAAGAC 3 cut(s) 40, 75, 172
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BsuI GTATCC 1 cut(s) 474
BsuRI GGCC 1 cut(s) 272
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 2 cut(s) 224, 262
BtsIMutI CAGTG 2 cut(s) 67, 467
Cac8I GCNNGC 2 cut(s) 24, 264
CseI GACGC 1 cut(s) 338
DdeI CTNAG 2 cut(s) 53, 347
DpnI GATC 4 cut(s) 114, 144, 413, 427
DpnII GATC 4 cut(s) 112, 142, 411, 425
EcoRII CCWGG 2 cut(s) 154, 476
FaiI YATR 4 cut(s) 42, 89, 234, 318
FauI CCCGC 1 cut(s) 24
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 20, 273
FokI GGATG 2 cut(s) 211, 269
Fsp4HI GCNGC 2 cut(s) 20, 273
FspBI CTAG 2 cut(s) 246, 443
GluI GCNGC 2 cut(s) 20, 273
GsaI CCCAGC 1 cut(s) 379
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 293
HgaI GACGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 32
HindII GTYRAC 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 293
HphI GGTGA 2 cut(s) 77, 152
Hpy166II GTNNAC 1 cut(s) 32
Hpy188I TCNGA 2 cut(s) 117, 416
Hpy188III TCNNGA 1 cut(s) 201
Hpy8I GTNNAC 1 cut(s) 32
Hpy99I CGWCG 1 cut(s) 36
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 2 cut(s) 209, 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 2 cut(s) 53, 347
HpySE526I ACGT 1 cut(s) 336
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 112, 142, 411, 425
LpnPI CCDG 9 cut(s) 36, 90, 141, 168, 241, 306, 321, 389, 463
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 246, 443
MaeII ACGT 1 cut(s) 336
MalI GATC 4 cut(s) 114, 144, 413, 427
MboI GATC 4 cut(s) 112, 142, 411, 425
MboII GAAGA 4 cut(s) 40, 80, 172, 421
MfeI CAATTG 2 cut(s) 189, 210
MflI RGATCY 1 cut(s) 425
MluCI AATT 4 cut(s) 37, 189, 210, 238
MnlI CCTC 4 cut(s) 62, 131, 187, 289
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 156, 478
MunI CAATTG 2 cut(s) 189, 210
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 2 cut(s) 156, 478
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeII GATC 4 cut(s) 112, 142, 411, 425
NlaIV GGNNCC 1 cut(s) 162
NspV TTCGAA 1 cut(s) 393
PctI GAATGC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 2 cut(s) 21, 274
PsiI TTATAA 1 cut(s) 42
Psp6I CCWGG 2 cut(s) 154, 476
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 2 cut(s) 154, 476
PspN4I GGNNCC 1 cut(s) 162
PsuI RGATCY 1 cut(s) 425
SalI GTCGAC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 2 cut(s) 20, 273
Sau3AI GATC 4 cut(s) 112, 142, 411, 425
ScrFI CCNGG 2 cut(s) 156, 478
SetI ASST 9 cut(s) 109, 142, 233, 251, 264, 339, 370, 381, 482
SfaNI GCATC 1 cut(s) 103
SfuI TTCGAA 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 37, 189, 210, 238
SsiI CCGC 3 cut(s) 17, 20, 273
SspI AATATT 1 cut(s) 303
SspMI CTAG 2 cut(s) 246, 443
StyD4I CCNGG 2 cut(s) 154, 476
TaiI ACGT 1 cut(s) 339
TaqI TCGA 6 cut(s) 31, 103, 128, 136, 200, 393
TasI AATT 4 cut(s) 37, 189, 210, 238
TauI GCSGC 2 cut(s) 22, 275
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 2 cut(s) 67, 474
TspDTI ATGAA 2 cut(s) 17, 213
TspGWI ACGGA 2 cut(s) 49, 419
TspRI CASTG 2 cut(s) 67, 474
XmiI GTMKAC 1 cut(s) 31
XspI CTAG 2 cut(s) 246, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.