RchiOBHm_Chr6g0255321

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025085
Physical Location & Seq
Forward (+)
10649172 .. 10649831
660 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22899

Sequence Viewer

Length: 615 bp
ATGGTGGTTGGGACGTTTAACATGAGAATGTGGTGGGTATGTGTTTCCTTGGAGTATGATCTGATTCAGATCACTAAATCAATCCTTGAGGCTACCACAAGCAAAGTTTGCACACTGTCAAATCTTGACTCTATTAAGAGGCAATTGAAAAAGATGATGGGAAAGTTTTTGCTCGTACTGGACAATATGTGGAATGAGACACCAGCAAGTAGGGTTCTGGTAACTGCTCGAAGTAAGACTGTTTCATCAATTGTTACCACTAATGGTGATCTTACTTGTTTTTCTCCAACTACTTTGTCTAAGGAGGATTGTTGGGAAATTATCAAGAAAAAACTGAAAACAGAAGTAGATAATCGTGAAGATTTGAAAGCTATTGGCTTACAGTTGGCAGAGAAGTGTAAAGGTCTTCTTTTGGCTGTTACAGTGATTGGAGATGCCTTGCATCTCAAATCAGAAGAGGTAGAGTGGGATGGGCTGCTGAAACTTTGGGACTTGCCCAAACATAACAACCAAGTCTACCGGGCACTTAAATTGAGCTACAATTATTTGCCAGCATATCTGAAAAGATGCTTCGCTTATTGCTCTATAATATTCCCCCGAGGTAAACAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

204

Amino Acids

23.33

Weight (kDa)

9.11

Isoelectric Point (pI)

28.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 2 - 112 4.2e-09 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025085)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0255321
rosa_multiflora Rmu_sc0011027.1_g000007
rosa_rugosa Rorug05G0557200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 516
AfaI GTAC 1 cut(s) 177
AgsI TTSAA 2 cut(s) 148, 367
AluBI AGCT 2 cut(s) 371, 537
AluI AGCT 2 cut(s) 371, 537
Alw26I GTCTC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 597
ApeKI GCWGC 1 cut(s) 475
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 597
BaeGI GKGCMC 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 462
BccI CCATC 2 cut(s) 151, 464
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 1 cut(s) 191
BisI GCNGC 1 cut(s) 476
BlsI GCNGC 1 cut(s) 477
Bme1390I CCNGG 1 cut(s) 521
BmeT110I CYCGRG 1 cut(s) 597
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 3 cut(s) 424, 451, 557
BpiI GAAGAC 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 48, 598
Bse1I ACTGG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 48, 598
BseGI GGATG 1 cut(s) 475
BseJI GATNNNNATC 1 cut(s) 68
BseNI ACTGG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 1 cut(s) 520
BslFI GGGAC 2 cut(s) 25, 503
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 2 cut(s) 25, 503
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 3 cut(s) 58, 69, 268
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 48, 598
BssMI GATC 3 cut(s) 58, 69, 268
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 4 cut(s) 117, 241, 384, 424
Bst6I CTCTTC 1 cut(s) 450
BstAPI GCANNNNNTGC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 3 cut(s) 61, 72, 271
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 3 cut(s) 58, 69, 268
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 526
BstV1I GCAGC 1 cut(s) 462
BstV2I GAAGAC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 2 cut(s) 113, 429
Cac8I GCNNGC 1 cut(s) 552
Csp6I GTAC 1 cut(s) 176
CviAII CATG 1 cut(s) 22
CviJI RGCY 6 cut(s) 92, 371, 378, 416, 475, 537
CviKI_1 RGCY 6 cut(s) 92, 371, 378, 416, 475, 537
CviQI GTAC 1 cut(s) 176
DdeI CTNAG 1 cut(s) 300
DpnI GATC 3 cut(s) 60, 71, 270
DpnII GATC 3 cut(s) 58, 69, 268
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 597
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 1 cut(s) 25
FaiI YATR 7 cut(s) 23, 40, 57, 188, 504, 556, 587
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FaqI GGGAC 2 cut(s) 25, 503
FatI CATG 1 cut(s) 21
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 1 cut(s) 476
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 1 cut(s) 476
GluI GCNGC 1 cut(s) 476
HapII CCGG 1 cut(s) 520
Hin1II CATG 1 cut(s) 25
HinfI GANTC 2 cut(s) 64, 128
HpaII CCGG 1 cut(s) 520
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 2 cut(s) 517, 605
Hpy188I TCNGA 4 cut(s) 63, 69, 454, 561
Hpy188III TCNNGA 3 cut(s) 125, 325, 356
Hpy8I GTNNAC 2 cut(s) 517, 605
HpyCH4III ACNGT 4 cut(s) 117, 241, 384, 424
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 2 cut(s) 111, 442
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 1 cut(s) 300
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 1 cut(s) 25
Kzo9I GATC 3 cut(s) 58, 69, 268
LpnPI CCDG 5 cut(s) 164, 203, 216, 533, 564
Lsp1109I GCAGC 1 cut(s) 462
LweI GCATC 3 cut(s) 424, 451, 557
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 3 cut(s) 220, 253, 418
MalI GATC 3 cut(s) 60, 71, 270
MboI GATC 3 cut(s) 58, 69, 268
MboII GAAGA 3 cut(s) 371, 398, 467
MfeI CAATTG 2 cut(s) 143, 249
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 6 cut(s) 143, 249, 318, 530, 541, 610
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 5 cut(s) 82, 132, 298, 451, 593
MseI TTAA 4 cut(s) 18, 135, 528, 613
MslI CAYNNNNRTG 1 cut(s) 26
MspI CCGG 1 cut(s) 520
MspR9I CCNGG 1 cut(s) 521
MunI CAATTG 2 cut(s) 143, 249
MwoI GCNNNNNNNGC 1 cut(s) 108
NciI CCSGG 1 cut(s) 521
NdeII GATC 3 cut(s) 58, 69, 268
NlaIII CATG 1 cut(s) 25
PfeI GAWTC 1 cut(s) 64
PkrI GCNGC 1 cut(s) 477
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
RsaI GTAC 1 cut(s) 177
RsaNI GTAC 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 4 cut(s) 18, 135, 528, 613
SatI GCNGC 1 cut(s) 476
Sau3AI GATC 3 cut(s) 58, 69, 268
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 1 cut(s) 526
SetI ASST 6 cut(s) 17, 373, 406, 462, 539, 604
SfaNI GCATC 3 cut(s) 424, 451, 557
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 6 cut(s) 143, 249, 318, 530, 541, 610
SspI AATATT 1 cut(s) 591
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 4 cut(s) 117, 241, 384, 424
TaiI ACGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 229
TasI AATT 6 cut(s) 143, 249, 318, 530, 541, 610
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 4 cut(s) 18, 135, 528, 613
Tru9I TTAA 4 cut(s) 18, 135, 528, 613
TscAI CASTG 2 cut(s) 120, 429
TseI GCWGC 1 cut(s) 475
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 2 cut(s) 120, 429
XmiI GTMKAC 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.