RchiOBHm_Chr6g0255821

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025066
Physical Location & Seq
Forward (+)
11235127 .. 11235984
858 bp
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UTR
Exon/CDS
Intron
PRQ22944

Sequence Viewer

Length: 297 bp
ATGGGCGAACTGGTCGAACCCTCTCCTGGAGTCTTTACATTTGAAATGCTTCAACCGCATTTCTGCGAATTGTCAGTATTACAGACAAGATTCGGAATCATGCGACCTAACACAGTGAACAAATATGGTGTTGTTCTTGATGACTTTGGCTTCAAAACTATGATTGACAAGTTAATAGAGGAGTTTATATGCCCTATATCTAGAGACATTCGTCTCGAATTTGTTATAAATTGGTGGAGCCACACTTTTCATGTGGATGACTCAGAAGTCCCCTTGAATGTTTGCTTGGGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

98

Amino Acids

11.37

Weight (kDa)

4.84

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 5 - 68 1.9e-20 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025066)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0255821
rosa_multiflora Rmu_sc0006792.1_g000028
rosa_wichuraiana Rw6G006870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AasI GACNNNNNNGTC 1 cut(s) 266
AciI CCGC 1 cut(s) 56
AcsI RAATTY 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 4 cut(s) 44, 53, 154, 277
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 1 cut(s) 269
Alw26I GTCTC 2 cut(s) 198, 218
ApoI RAATTY 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 27
BcoDI GTCTC 2 cut(s) 198, 218
BfaI CTAG 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 27
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 27
BoxI GACNNNNGTC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 15
BseBI CCWGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 2 cut(s) 198, 218
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 254
BspACI CCGC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 239
BsrI ACTGG 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 262
BstF5I GGATG 1 cut(s) 262
BstMAI GTCTC 2 cut(s) 198, 218
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 27
BstPAI GACNNNNGTC 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 25
BtsCI GGATG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 120
CviAII CATG 2 cut(s) 100, 251
CviJI RGCY 2 cut(s) 150, 240
CviKI_1 RGCY 2 cut(s) 150, 240
DdeI CTNAG 1 cut(s) 262
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 2 cut(s) 103, 254
FaiI YATR 8 cut(s) 101, 126, 161, 188, 190, 197, 227, 252
FaqI GGGAC 1 cut(s) 254
FatI CATG 2 cut(s) 99, 250
FokI GGATG 1 cut(s) 269
FspBI CTAG 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 48
Hin1II CATG 2 cut(s) 103, 254
HinfI GANTC 4 cut(s) 30, 90, 96, 260
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 2 cut(s) 95, 265
Hpy188III TCNNGA 3 cut(s) 137, 201, 215
Hpy8I GTNNAC 1 cut(s) 118
HpyCH4III ACNGT 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 1 cut(s) 262
Hsp92II CATG 2 cut(s) 103, 254
LmnI GCTCC 1 cut(s) 237
LpnPI CCDG 2 cut(s) 12, 39
MaeI CTAG 1 cut(s) 201
MluCI AATT 3 cut(s) 68, 218, 229
MlyI GAGTC 2 cut(s) 39, 254
MnlI CCTC 2 cut(s) 31, 172
MroXI GAANNNNTTC 1 cut(s) 48
MseI TTAA 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 55
NlaIII CATG 2 cut(s) 103, 254
NlaIV GGNNCC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 2 cut(s) 90, 96
PfoI TCCNGGA 1 cut(s) 25
PleI GAGTC 2 cut(s) 38, 254
PpsI GAGTC 2 cut(s) 38, 254
PshAI GACNNNNGTC 1 cut(s) 210
PsiI TTATAA 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 255
SaqAI TTAA 1 cut(s) 173
SchI GAGTC 2 cut(s) 39, 254
ScrFI CCNGG 1 cut(s) 27
SetI ASST 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 3 cut(s) 68, 218, 229
SsiI CCGC 1 cut(s) 56
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 115
TaqI TCGA 2 cut(s) 15, 216
TasI AATT 3 cut(s) 68, 218, 229
TfiI GAWTC 2 cut(s) 90, 96
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 1 cut(s) 120
TspDTI ATGAA 1 cut(s) 239
TspRI CASTG 1 cut(s) 120
XapI RAATTY 1 cut(s) 218
XbaI TCTAGA 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.