Rw6G006870

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0025066
Physical Location & Seq
Forward (+)
10981341 .. 10987293
5953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006870.1

Sequence Viewer

Length: 723 bp
ATGTCAACAAAGAGCAGATTGCCGCCTGGAAAAGATGCGATCCAGGTTGAATTGGATTCACTAACAAAGAGACAGGTATTTGGGCCTGTAACGCAGACCCCCCAAGTGTTTTTAACGAGGCAACGGATGAAGCGTCACCACCAATTTCTACACGAGAATTTATACACTGTGCAAGCTGAACATTTTTTTGTCCCTGCATTTCTCAATGCCATCAATGAGGATACAGAGAATAGTTTTAGAATTGCAATGGGCGAACTGGTCGAACCCTCTCCTGGAGTCTTTACATTTGAAATGCTTCAACCGCATTTCTGCGAATTGTCAGTATTACAGACAAGATTCGGAATCATGCGACCAAACACAGTGAGCAAATATGGTGTTGTTGTTGATGACTTTGGCTTCGAAACTATGATTGACAAGTTAATAGATGAGTTTATATGCCCCATATCTAGAGTTGTCTTTCCAGAAGTTGGTGGAGCCACATTGGATTCTCATCATGGTTTTTGGCTTTGTATTGTAGCTTTTCATGTGGATGACTCAGAAGTCCCCTTGAATATGTATTTTCGAGGTGTTCGATGTCATAAGCATGTGAATTCAGAGATACTTGCAGAGGATACTGTGAAGAAGAAGCGAAGCAGGTGTGGAGCCTGCAGTCTTGAAAAATTAATCATTGCTTGCAACTTGCAAATGCATCTGACTATCTTCTTGCGATGGAGTATCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.47

Weight (kDa)

6.59

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 44 - 158 5.1e-33 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025066)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0255821
rosa_multiflora Rmu_sc0006792.1_g000028
rosa_wichuraiana Rw6G006870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 624
AasI GACNNNNNNGTC 1 cut(s) 539
Acc36I ACCTGC 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 467
AciI CCGC 2 cut(s) 23, 302
AclWI GGATC 1 cut(s) 34
AcsI RAATTY 2 cut(s) 157, 589
AfiI CCNNNNNNNGG 2 cut(s) 272, 467
AgsI TTSAA 5 cut(s) 50, 290, 299, 550, 656
AjnI CCWGG 3 cut(s) 25, 42, 271
AluBI AGCT 2 cut(s) 176, 518
AluI AGCT 2 cut(s) 176, 518
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 1 cut(s) 34
AoxI GGCC 1 cut(s) 83
ApoI RAATTY 2 cut(s) 157, 589
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
AseI ATTAAT 1 cut(s) 662
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 128
AsuII TTCGAA 1 cut(s) 399
BauI CACGAG 1 cut(s) 152
BccI CCATC 2 cut(s) 218, 702
BciT130I CCWGG 3 cut(s) 27, 44, 273
BciVI GTATCC 2 cut(s) 214, 604
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 646
BfuAI ACCTGC 1 cut(s) 624
BfuI GTATCC 2 cut(s) 214, 604
BisI GCNGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 24
Bme1390I CCNGG 3 cut(s) 27, 44, 273
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 2 cut(s) 475, 643
BmrFI CCNGG 3 cut(s) 27, 44, 273
BmsI GCATC 2 cut(s) 25, 697
BpmI CTGGAG 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 489
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 467
Bse1I ACTGG 1 cut(s) 261
Bse3DI GCAATG 2 cut(s) 252, 666
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 3 cut(s) 27, 44, 273
BseGI GGATG 2 cut(s) 132, 535
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 2 cut(s) 272, 467
BseMI GCAATG 2 cut(s) 252, 666
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 261
BshFI GGCC 1 cut(s) 85
BslFI GGGAC 2 cut(s) 176, 527
BslI CCNNNNNNNGG 2 cut(s) 272, 467
BsmAI GTCTC 1 cut(s) 64
BsmFI GGGAC 2 cut(s) 176, 527
BsnI GGCC 1 cut(s) 85
Bsp119I TTCGAA 1 cut(s) 399
Bsp143I GATC 1 cut(s) 39
BspACI CCGC 2 cut(s) 23, 302
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 548
BspLI GGNNCC 2 cut(s) 475, 643
BspMAI CTGCAG 1 cut(s) 650
BspMI ACCTGC 1 cut(s) 624
BspPI GGATC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 399
BsrDI GCAATG 2 cut(s) 252, 666
BsrI ACTGG 1 cut(s) 261
BssMI GATC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 3 cut(s) 27, 44, 273
Bst4CI ACNGT 3 cut(s) 169, 361, 616
BstBI TTCGAA 1 cut(s) 399
BstC8I GCNNGC 3 cut(s) 174, 646, 673
BstDEI CTNAG 1 cut(s) 535
BstF5I GGATG 2 cut(s) 132, 535
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 1 cut(s) 39
BstMWI GCNNNNNNNGC 2 cut(s) 91, 301
BstNI CCWGG 3 cut(s) 27, 44, 273
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 3 cut(s) 25, 42, 271
BstSFI CTRYAG 1 cut(s) 646
BsuI GTATCC 2 cut(s) 214, 604
BsuRI GGCC 1 cut(s) 85
BtsCI GGATG 2 cut(s) 132, 535
BtsIMutI CAGTG 2 cut(s) 165, 366
BveI ACCTGC 1 cut(s) 624
Cac8I GCNNGC 3 cut(s) 174, 646, 673
Cfr13I GGNCC 1 cut(s) 83
CseI GACGC 1 cut(s) 122
CviAII CATG 4 cut(s) 346, 494, 524, 584
CviJI RGCY 7 cut(s) 85, 176, 396, 476, 505, 518, 644
CviKI_1 RGCY 7 cut(s) 85, 176, 396, 476, 505, 518, 644
DdeI CTNAG 1 cut(s) 535
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
DrdI GACNNNNNNGTC 1 cut(s) 539
DseDI GACNNNNNNGTC 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 589
EcoRII CCWGG 3 cut(s) 25, 42, 271
EcoT22I ATGCAT 1 cut(s) 690
FaeI CATG 4 cut(s) 349, 497, 527, 587
FaqI GGGAC 2 cut(s) 176, 527
FatI CATG 4 cut(s) 345, 493, 523, 583
Fnu4HI GCNGC 1 cut(s) 23
FokI GGATG 2 cut(s) 139, 542
Fsp4HI GCNGC 1 cut(s) 23
FspBI CTAG 1 cut(s) 447
GluI GCNGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 1 cut(s) 85
HgaI GACGC 1 cut(s) 122
Hin1II CATG 4 cut(s) 349, 497, 527, 587
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 6 cut(s) 56, 276, 336, 342, 485, 533
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 5 cut(s) 341, 538, 595, 693, 719
Hpy188III TCNNGA 3 cut(s) 447, 461, 653
Hpy8I GTNNAC 1 cut(s) 6
HpyCH4III ACNGT 3 cut(s) 169, 361, 616
HpyCH4V TGCA 8 cut(s) 172, 197, 245, 605, 648, 675, 682, 688
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 301
HpyF3I CTNAG 1 cut(s) 535
Hsp92II CATG 4 cut(s) 349, 497, 527, 587
Kzo9I GATC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 473, 641
LweI GCATC 2 cut(s) 25, 697
MaeI CTAG 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 88, 134
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 3 cut(s) 631, 634, 691
MluCI AATT 7 cut(s) 50, 143, 157, 240, 314, 589, 659
MlyI GAGTC 2 cut(s) 285, 527
MnlI CCTC 5 cut(s) 111, 211, 277, 557, 601
Mph1103I ATGCAT 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 3 cut(s) 113, 419, 662
MslI CAYNNNNRTG 2 cut(s) 528, 582
MspR9I CCNGG 3 cut(s) 27, 44, 273
MvaI CCWGG 3 cut(s) 27, 44, 273
MwoI GCNNNNNNNGC 2 cut(s) 91, 301
NdeII GATC 1 cut(s) 39
NlaIII CATG 4 cut(s) 349, 497, 527, 587
NlaIV GGNNCC 2 cut(s) 475, 643
NmuCI GTSAC 1 cut(s) 134
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 1 cut(s) 399
PaqCI CACCTGC 1 cut(s) 624
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 568
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 4 cut(s) 56, 336, 342, 485
PflMI CCANNNNNTGG 1 cut(s) 467
PfoI TCCNGGA 1 cut(s) 271
PkrI GCNGC 1 cut(s) 24
PleI GAGTC 2 cut(s) 284, 527
PpsI GAGTC 2 cut(s) 284, 527
PshBI ATTAAT 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 25, 42, 271
PspGI CCWGG 3 cut(s) 25, 42, 271
PspN4I GGNNCC 2 cut(s) 475, 643
PspPI GGNCC 1 cut(s) 83
PstI CTGCAG 1 cut(s) 650
RseI CAYNNNNRTG 2 cut(s) 528, 582
SaqAI TTAA 3 cut(s) 113, 419, 662
SatI GCNGC 1 cut(s) 23
Sau3AI GATC 1 cut(s) 39
Sau96I GGNCC 1 cut(s) 83
SchI GAGTC 2 cut(s) 285, 527
ScrFI CCNGG 3 cut(s) 27, 44, 273
SetI ASST 6 cut(s) 48, 78, 178, 520, 568, 638
SfaNI GCATC 2 cut(s) 25, 697
SfcI CTRYAG 1 cut(s) 646
SfuI TTCGAA 1 cut(s) 399
SmiMI CAYNNNNRTG 2 cut(s) 528, 582
Sse9I AATT 7 cut(s) 50, 143, 157, 240, 314, 589, 659
SsiI CCGC 2 cut(s) 23, 302
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 25, 42, 271
TaaI ACNGT 3 cut(s) 169, 361, 616
TaqI TCGA 4 cut(s) 261, 399, 562, 571
TasI AATT 7 cut(s) 50, 143, 157, 240, 314, 589, 659
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 4 cut(s) 56, 336, 342, 485
Tru1I TTAA 3 cut(s) 113, 419, 662
Tru9I TTAA 3 cut(s) 113, 419, 662
TscAI CASTG 2 cut(s) 172, 366
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 2 cut(s) 143, 512
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 2 cut(s) 172, 366
Van91I CCANNNNNTGG 1 cut(s) 467
VspI ATTAAT 1 cut(s) 662
XapI RAATTY 2 cut(s) 157, 589
XbaI TCTAGA 1 cut(s) 446
XceI RCATGY 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.