RchiOBHm_Chr6g0261401

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0022790
Physical Location & Seq
Reverse (-)
16249551 .. 16252291
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23443

Sequence Viewer

Length: 651 bp
ATGGATCCTGGGAACCAAAAGAAGAAGAGAAAAGATATGGGTCCCCCATCAAAAACAAGAAGCTTAATCAGCAATTCTCCGCCCCTAAAAAGAAGAAGGTTACTCAACATGGATTCTGTTTCTACTCAAGGGGCCTCTTCTTCCTCTTCTCCTTCTTCCTCCCATTCATTGACATACGATGTGTTCTTGAGTATTAGAAGTGAGGATAGAGGCAGCAAATTCGCCCGCAAATTACACAGAAATCTGGTTGGGAAGGGGATGAGCACCTTCAATCATCGCATTCAGGATCCAGAGGGACACGCAAAAGCAATTGAAGAATCAAGTAGTTTTATTGTCCTGCTCTCTAAAAAGTATGCTTACTCGGAGAGTTGTTTAGATGAACTTGTTCATATCCTTGAGTGCAAGAAATCTAAGCAACAACTGGTTTGGCCAATATTTTACAAGGTAGAAGACCCCTTCGAGGTAGAACACCAGATCGGAAGTTTTGGTGAAGCACTAGCAAAACATGGCAACTTCAAGAATAACAGGGAGAAGGTGTCGAGATGGACAAGAGCGCTGTCGGAAGTTGCACATCTATCTGGGTGGACTTTCACAAGTGGTTCGAGAGGATACATGCAAGATTGGCTGTCCCACTGTTTGTACGGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

216

Amino Acids

24.46

Weight (kDa)

9.57

Isoelectric Point (pI)

64.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 59 - 199 3.7e-30 TIR domain
TIR_2 PF13676 61 - 152 2.6e-09 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022790)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0261131 RchiOBHm_Chr6g0261401 RchiOBHm_Chr6g0261461 RchiOBHm_Chr6g0261661

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 80, 226
AclWI GGATC 3 cut(s) 12, 281, 294
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 1 cut(s) 218
AfaI GTAC 1 cut(s) 641
AfeI AGCGCT 1 cut(s) 555
AfiI CCNNNNNNNGG 1 cut(s) 460
AgsI TTSAA 3 cut(s) 271, 314, 517
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 1 cut(s) 63
AluI AGCT 1 cut(s) 63
Alw21I GWGCWC 1 cut(s) 266
AlwI GGATC 3 cut(s) 12, 281, 294
Aor51HI AGCGCT 1 cut(s) 555
AoxI GGCC 2 cut(s) 132, 428
ApeKI GCWGC 2 cut(s) 213, 645
ApoI RAATTY 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 41, 132
AsuHPI GGTGA 1 cut(s) 500
AvaII GGWCC 1 cut(s) 41
BalI TGGCCA 1 cut(s) 430
BamHI GGATCC 2 cut(s) 4, 286
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 266
BbvI GCAGC 2 cut(s) 225, 632
BccI CCATC 2 cut(s) 55, 537
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 1 cut(s) 9
BciVI GTATCC 1 cut(s) 602
BfaI CTAG 1 cut(s) 497
BfoI RGCGCY 1 cut(s) 557
BfuI GTATCC 1 cut(s) 602
BisI GCNGC 2 cut(s) 214, 646
BlsI GCNGC 2 cut(s) 215, 647
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 1 cut(s) 41
BmgT120I GGNCC 2 cut(s) 41, 132
BmiI GGNNCC 6 cut(s) 6, 14, 42, 43, 133, 288
BmrFI CCNGG 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 456
BpuEI CTTGAG 3 cut(s) 111, 208, 416
BsaJI CCNNGG 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 521, 551
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 8
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 460
BseNI ACTGG 1 cut(s) 426
BseXI GCAGC 2 cut(s) 225, 632
BshFI GGCC 2 cut(s) 134, 430
BsiHKAI GWGCWC 1 cut(s) 266
BslFI GGGAC 3 cut(s) 27, 309, 613
BslI CCNNNNNNNGG 1 cut(s) 460
BsmFI GGGAC 3 cut(s) 27, 309, 613
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 2 cut(s) 134, 430
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 3 cut(s) 4, 286, 474
BspACI CCGC 2 cut(s) 80, 226
BspANI GGCC 2 cut(s) 134, 430
BspLI GGNNCC 6 cut(s) 6, 14, 42, 43, 133, 288
BspPI GGATC 3 cut(s) 12, 281, 294
BsrI ACTGG 1 cut(s) 426
BssECI CCNNGG 1 cut(s) 8
BssMI GATC 3 cut(s) 4, 286, 474
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 635
Bst6I CTCTTC 3 cut(s) 20, 142, 151
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 264
BstH2I RGCGCY 1 cut(s) 557
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 3 cut(s) 7, 289, 477
BstMBI GATC 3 cut(s) 4, 286, 474
BstMWI GCNNNNNNNGC 2 cut(s) 69, 622
BstNI CCWGG 1 cut(s) 9
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 7
BstV1I GCAGC 2 cut(s) 225, 632
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 2 cut(s) 4, 286
BstYI RGATCY 2 cut(s) 4, 286
BsuI GTATCC 1 cut(s) 602
BsuRI GGCC 2 cut(s) 134, 430
BtgZI GCGATG 1 cut(s) 260
BtsCI GGATG 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 631
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 41, 132
Csp6I GTAC 1 cut(s) 640
CviAII CATG 3 cut(s) 109, 506, 613
CviJI RGCY 5 cut(s) 63, 134, 430, 625, 645
CviKI_1 RGCY 5 cut(s) 63, 134, 430, 625, 645
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 1 cut(s) 411
DpnI GATC 3 cut(s) 6, 288, 476
DpnII GATC 3 cut(s) 4, 286, 474
EaeI YGGCCR 1 cut(s) 428
Eam1104I CTCTTC 3 cut(s) 20, 142, 151
EarI CTCTTC 3 cut(s) 20, 142, 151
EciI GGCGGA 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 41
Eco47III AGCGCT 1 cut(s) 555
EcoO109I RGGNCCY 2 cut(s) 41, 132
EcoRII CCWGG 1 cut(s) 7
FaeI CATG 3 cut(s) 112, 509, 616
FaiI YATR 7 cut(s) 38, 110, 175, 354, 390, 507, 614
FaqI GGGAC 3 cut(s) 27, 309, 613
FatI CATG 3 cut(s) 108, 505, 612
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 2 cut(s) 214, 646
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 2 cut(s) 214, 646
FspBI CTAG 1 cut(s) 497
GlaI GCGC 1 cut(s) 555
GluI GCNGC 2 cut(s) 214, 646
HaeII RGCGCY 1 cut(s) 557
HaeIII GGCC 2 cut(s) 134, 430
HhaI GCGC 1 cut(s) 556
Hin1II CATG 3 cut(s) 112, 509, 616
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 2 cut(s) 113, 317
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 585
Hpy188I TCNGA 3 cut(s) 364, 479, 562
Hpy188III TCNNGA 6 cut(s) 187, 284, 290, 517, 540, 603
Hpy8I GTNNAC 1 cut(s) 585
HpyAV CCTTC 6 cut(s) 90, 162, 247, 277, 466, 526
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4V TGCA 3 cut(s) 402, 569, 616
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 622
HpyF3I CTNAG 1 cut(s) 411
Hsp92II CATG 3 cut(s) 112, 509, 616
HspAI GCGC 1 cut(s) 554
KflI GGGWCCC 1 cut(s) 41
Kzo9I GATC 3 cut(s) 4, 286, 474
LpnPI CCDG 9 cut(s) 21, 230, 269, 303, 350, 407, 485, 511, 564
Lsp1109I GCAGC 2 cut(s) 225, 632
MaeI CTAG 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 99
MalI GATC 3 cut(s) 6, 288, 476
MboI GATC 3 cut(s) 4, 286, 474
MboII GAAGA 9 cut(s) 34, 37, 105, 129, 132, 138, 147, 326, 461
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 2 cut(s) 4, 286
MhlI GDGCHC 1 cut(s) 266
MlsI TGGCCA 1 cut(s) 430
MluCI AATT 4 cut(s) 73, 218, 230, 309
MluNI TGGCCA 1 cut(s) 430
MmeI TCCRAC 1 cut(s) 540
MnlI CCTC 8 cut(s) 145, 154, 169, 196, 203, 286, 454, 599
Mox20I TGGCCA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 384
MscI TGGCCA 1 cut(s) 430
MseI TTAA 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 9
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 279
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 2 cut(s) 69, 622
NdeII GATC 3 cut(s) 4, 286, 474
NlaIII CATG 3 cut(s) 112, 509, 616
NlaIV GGNNCC 6 cut(s) 6, 14, 42, 43, 133, 288
NspI RCATGY 1 cut(s) 616
PctI GAATGC 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 2 cut(s) 113, 317
PkrI GCNGC 2 cut(s) 215, 647
PpuMI RGGWCCY 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 6 cut(s) 6, 14, 42, 43, 133, 288
PspPI GGNCC 2 cut(s) 41, 132
PspPPI RGGWCCY 1 cut(s) 41
PsuI RGATCY 2 cut(s) 4, 286
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 2 cut(s) 214, 646
Sau3AI GATC 3 cut(s) 4, 286, 474
Sau96I GGNCC 2 cut(s) 41, 132
ScrFI CCNGG 1 cut(s) 9
SduI GDGCHC 1 cut(s) 266
SetI ASST 6 cut(s) 65, 101, 269, 447, 465, 537
SinI GGWCC 1 cut(s) 41
SmlI CTYRAG 3 cut(s) 126, 187, 395
SmoI CTYRAG 3 cut(s) 126, 187, 395
Sse9I AATT 4 cut(s) 73, 218, 230, 309
SsiI CCGC 2 cut(s) 80, 226
SspI AATATT 1 cut(s) 435
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 1 cut(s) 7
TaaI ACNGT 1 cut(s) 635
TaqI TCGA 3 cut(s) 459, 539, 602
TasI AATT 4 cut(s) 73, 218, 230, 309
TfiI GAWTC 2 cut(s) 113, 317
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 1 cut(s) 638
TseI GCWGC 2 cut(s) 213, 645
TspDTI ATGAA 3 cut(s) 156, 377, 393
TspRI CASTG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 218
XceI RCATGY 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.