RchiOBHm_Chr6g0277691

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0030359
Physical Location & Seq
Forward (+)
40199516 .. 40201479
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24912

Sequence Viewer

Length: 1485 bp
ATGGAGAGAATTGCTTGGTTCTTCCTGCTGCAGTACTGTTGTTGTATGGCGGCTACCTGCGTAACCATGGCAGCACATGTACAAACCAACATCAGCATAGACCAGTCTGCTCTTCTTGCTTTCAAAGCCAGTATCACCGATGATCCTCATAAACTGATCTTCACCAACTGGTCAACCATCGTCCCAGTTTGTAACTGGGTTGGCGTTACTTGTGCTGCTCGCCACCACAGAGTTGCGGTCTTGAACTTATCTTATTTTGGTCTCACTGGCACCATTCCTCCGGAGCTAGGAAACCTTTCATTTCTTGTGGAACTTCGCCTCAGAAATAACAGTTTTCATGGTACCTTGCCTCAGGAATTGGCTAGCCTGCACCGGTTGAGGTTGATTAGCTTGGGATATAACAAGTTTATGGGAACCATTCCCTCATGGTTCGGGTCCTTATCCAAACTTCAAACATTTAATTTGTATGGTAATCAGTTTTCGGGTTCCATACCAGCAACCATCTTGAACTTATCTGCACTTCAAGTAATTGATCTGACGTATAACCAACTCTCAGGTAACATACCAAGAGAGATTGGAAACATAACAATGTTGAAGGAAATATACCTGGACAGTAATAAATTCACAGAAATTCCTAATGAGATTGGCAGTTTAGATCAATTGGAAATATTGTTTGTGCAATTGAATGCTTTAAAAGGGTCTATTCCTATGGTTGTTTTCAACATGTCTTCTTTGACTATGTTGGCTCTATATGGAAACAATTTAAATGGTAGTCTTCCGGACAATATATGTCAACATCTTCCGAGTATTCAAGATTTGACTCTGGGTTACAACCAATTCGACGGTCCACTTCCATCGAAATTGTGGGAATGCAAAGAACTCCTTCAATTGGAAGTACCACGCAACAGTTTCAGTGGAGAAATACCAAATACTTTTGGGAACTTGACTCAGATACAGACAATTGACCTTGGCTTGAACTATTTGACAGGTCCTATACCACATCAGATTGGTCATCTTCCAAATTTAGAGATATTGAACCTCGGTGGGAATAACTTCAATGAGGTCATCCCATCCTTAATCTTCAATATGTCCACAATAGCAGTGATATCGCTTTTCGACAATCAGCTCTCAGGCAACCTTCCACCAAACACAGGCCTTGGCCTTCCAAACCTACAACGCTTCTATCTAGGAGGAAATAAACTTAGCGGAGCAATCCCCAAATCTTTGTCCAATGCTTCTAACCTGGAGGTCATAGACCTAGGCAGCAACCTATTATTTGGCGTTATTCCTAGCACCCTATGTACCTTGACAAAACTTCAGCTGCTTCAACTATTTGATAATAACCTGACCGTCAGTACTTCGACTCCAGAACTGGATGTTCTCTTTTGTTTGGCAAATCTTCGCAACTTGAGGAGACTAGACTTCTCAGTAAATCCATTAGATGCCAACCTTCCGATTTCCTTTACGAATACATCTCTTCAATAA

Protein Analysis

494

Amino Acids

54.43

Weight (kDa)

5.58

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 71 1.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 178 5e-07 Leucine-rich repeat region
LRR_8 PF13855 125 - 184 6.2e-08 Leucine rich repeat
LRR_14 PF23598 168 - 278 8.2e-10 Leucine-rich repeat region
LRR_8 PF13855 297 - 352 5.1e-07 Leucine rich repeat
LRR_8 PF13855 340 - 401 5.8e-07 Leucine rich repeat
LRR_8 PF13855 389 - 449 1.5e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0030359)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0277691
rosa_samantha Rh6DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 269, 341
AccIII TCCGGA 2 cut(s) 280, 778
AciI CCGC 3 cut(s) 50, 236, 1206
AclWI GGATC 1 cut(s) 137
AcsI RAATTY 3 cut(s) 620, 630, 1021
AcuI CTGAAG 1 cut(s) 1301
AfaI GTAC 6 cut(s) 35, 81, 343, 897, 1303, 1357
AfiI CCNNNNNNNGG 3 cut(s) 287, 889, 1151
AflIII ACRYGT 2 cut(s) 76, 723
AgeI ACCGGT 1 cut(s) 372
AjnI CCWGG 2 cut(s) 606, 1242
AluBI AGCT 4 cut(s) 286, 390, 1126, 1321
AluI AGCT 4 cut(s) 286, 390, 1126, 1321
Alw26I GTCTC 2 cut(s) 266, 1408
AlwI GGATC 1 cut(s) 137
Aor13HI TCCGGA 2 cut(s) 280, 778
AoxI GGCC 2 cut(s) 1153, 1159
ApeKI GCWGC 5 cut(s) 28, 71, 215, 1263, 1321
ApoI RAATTY 3 cut(s) 620, 630, 1021
AsiGI ACCGGT 1 cut(s) 372
Asp700I GAANNNNTTC 3 cut(s) 295, 882, 1052
Asp718I GGTACC 1 cut(s) 341
AspA2I CCTAGG 1 cut(s) 1258
AspS9I GGNCC 3 cut(s) 435, 845, 989
AsuHPI GGTGA 2 cut(s) 127, 154
AsuNHI GCTAGC 1 cut(s) 362
AvaII GGWCC 3 cut(s) 435, 845, 989
AvrII CCTAGG 1 cut(s) 1258
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 2 cut(s) 269, 341
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BbsI GAAGAC 2 cut(s) 720, 767
BbvI GCAGC 5 cut(s) 15, 83, 202, 1275, 1308
BccI CCATC 4 cut(s) 185, 509, 862, 1078
BcgI CGANNNNNNTGC 2 cut(s) 1089, 1123
BciT130I CCWGG 2 cut(s) 608, 1244
BcoDI GTCTC 2 cut(s) 266, 1408
BfaI CTAG 6 cut(s) 287, 363, 1187, 1259, 1290, 1418
BfmI CTRYAG 1 cut(s) 29
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 6 cut(s) 29, 51, 72, 216, 1264, 1322
BlnI CCTAGG 1 cut(s) 1258
BlsI GCNGC 6 cut(s) 30, 52, 73, 217, 1265, 1323
BmcAI AGTACT 2 cut(s) 35, 1357
Bme1390I CCNGG 2 cut(s) 608, 1244
Bme18I GGWCC 3 cut(s) 435, 845, 989
BmgT120I GGNCC 3 cut(s) 435, 845, 989
BmiI GGNNCC 5 cut(s) 271, 343, 415, 436, 487
BmrFI CCNGG 2 cut(s) 608, 1244
BmrI ACTGGG 2 cut(s) 179, 205
BmsI GCATC 1 cut(s) 1432
BmtI GCTAGC 1 cut(s) 366
BmuI ACTGGG 2 cut(s) 179, 205
BpiI GAAGAC 2 cut(s) 720, 767
BpmI CTGGAG 2 cut(s) 1265, 1350
BpuEI CTTGAG 1 cut(s) 1429
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 5 cut(s) 66, 967, 1039, 1156, 1258
BsaWI WCCGGW 3 cut(s) 280, 372, 778
BsaXI ACNNNNNCTCC 4 cut(s) 262, 292, 1348, 1378
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 889, 1151
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 7 cut(s) 103, 129, 173, 185, 200, 271, 1377
Bse21I CCTNAGG 1 cut(s) 351
BseAI TCCGGA 2 cut(s) 280, 778
BseBI CCWGG 2 cut(s) 608, 1244
BseDI CCNNGG 5 cut(s) 66, 967, 1039, 1156, 1258
BseGI GGATG 3 cut(s) 1065, 1070, 1381
BseLI CCNNNNNNNGG 3 cut(s) 287, 889, 1151
BseMII CTCAG 6 cut(s) 334, 365, 567, 962, 1143, 1440
BseNI ACTGG 7 cut(s) 103, 129, 173, 185, 200, 271, 1377
BseRI GAGGAG 1 cut(s) 1426
BseXI GCAGC 5 cut(s) 15, 83, 202, 1275, 1308
BsgI GTGCAG 2 cut(s) 353, 501
BshFI GGCC 2 cut(s) 1155, 1161
BshNI GGYRCC 2 cut(s) 269, 341
BshTI ACCGGT 1 cut(s) 372
BsiSI CCGG 3 cut(s) 281, 373, 779
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 3 cut(s) 287, 889, 1151
BsmAI GTCTC 2 cut(s) 266, 1408
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 691, 875
BsnI GGCC 2 cut(s) 1155, 1161
Bso31I GGTCTC 1 cut(s) 266
Bsp13I TCCGGA 2 cut(s) 280, 778
Bsp1407I TGTACA 1 cut(s) 79
Bsp143I GATC 4 cut(s) 142, 156, 532, 655
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 3 cut(s) 50, 236, 1206
BspANI GGCC 2 cut(s) 1155, 1161
BspCNI CTCAG 6 cut(s) 333, 364, 566, 961, 1142, 1439
BspEI TCCGGA 2 cut(s) 280, 778
BspLI GGNNCC 5 cut(s) 271, 343, 415, 436, 487
BspMAI CTGCAG 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 65
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 137
BspQI GCTCTTC 1 cut(s) 117
BspT107I GGYRCC 2 cut(s) 269, 341
BspTNI GGTCTC 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 372
BsrGI TGTACA 1 cut(s) 79
BsrI ACTGG 7 cut(s) 103, 129, 173, 185, 200, 271, 1377
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 5 cut(s) 66, 967, 1039, 1156, 1258
BssMI GATC 4 cut(s) 142, 156, 532, 655
BssT1I CCWWGG 4 cut(s) 66, 967, 1156, 1258
Bst2UI CCWGG 2 cut(s) 608, 1244
Bst4CI ACNGT 6 cut(s) 38, 332, 614, 845, 908, 1351
Bst6I CTCTTC 2 cut(s) 117, 1482
BstAUI TGTACA 1 cut(s) 79
BstC8I GCNNGC 3 cut(s) 220, 364, 368
BstDEI CTNAG 7 cut(s) 320, 351, 553, 948, 1129, 1202, 1426
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 3 cut(s) 1065, 1070, 1381
BstKTI GATC 4 cut(s) 145, 159, 535, 658
BstMAI GTCTC 2 cut(s) 266, 1408
BstMBI GATC 4 cut(s) 142, 156, 532, 655
BstMWI GCNNNNNNNGC 2 cut(s) 116, 125
BstNI CCWGG 2 cut(s) 608, 1244
BstNSI RCATGY 2 cut(s) 80, 727
BstSCI CCNGG 2 cut(s) 606, 1242
BstSFI CTRYAG 1 cut(s) 29
BstV1I GCAGC 5 cut(s) 15, 83, 202, 1275, 1308
BstV2I GAAGAC 2 cut(s) 720, 767
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 2 cut(s) 1155, 1161
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 1065, 1070, 1381
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 3 cut(s) 264, 919, 1107
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 3 cut(s) 220, 364, 368
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 3 cut(s) 435, 845, 989
Csp6I GTAC 6 cut(s) 34, 80, 342, 896, 1302, 1356
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 5 cut(s) 67, 77, 338, 426, 724
CviQI GTAC 6 cut(s) 34, 80, 342, 896, 1302, 1356
DdeI CTNAG 7 cut(s) 320, 351, 553, 948, 1129, 1202, 1426
DpnI GATC 4 cut(s) 144, 158, 534, 657
DpnII GATC 4 cut(s) 142, 156, 532, 655
DraI TTTAAA 2 cut(s) 693, 765
Eam1104I CTCTTC 2 cut(s) 117, 1482
EarI CTCTTC 2 cut(s) 117, 1482
Eco130I CCWWGG 4 cut(s) 66, 967, 1156, 1258
Eco147I AGGCCT 1 cut(s) 1155
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 1 cut(s) 1107
Eco47I GGWCC 3 cut(s) 435, 845, 989
Eco57I CTGAAG 1 cut(s) 1301
Eco81I CCTNAGG 1 cut(s) 351
EcoO109I RGGNCCY 2 cut(s) 435, 989
EcoRII CCWGG 2 cut(s) 606, 1242
EcoRV GATATC 1 cut(s) 1107
EcoT14I CCWWGG 4 cut(s) 66, 967, 1156, 1258
ErhI CCWWGG 4 cut(s) 66, 967, 1156, 1258
FaeI CATG 5 cut(s) 70, 80, 341, 429, 727
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 1 cut(s) 167
FatI CATG 5 cut(s) 66, 76, 337, 425, 723
Fnu4HI GCNGC 6 cut(s) 29, 51, 72, 216, 1264, 1322
FokI GGATG 3 cut(s) 1052, 1057, 1388
Fsp4HI GCNGC 6 cut(s) 29, 51, 72, 216, 1264, 1322
FspBI CTAG 6 cut(s) 287, 363, 1187, 1259, 1290, 1418
GluI GCNGC 6 cut(s) 29, 51, 72, 216, 1264, 1322
GsuI CTGGAG 2 cut(s) 1265, 1350
HaeIII GGCC 2 cut(s) 1155, 1161
HapII CCGG 3 cut(s) 281, 373, 779
Hin1II CATG 5 cut(s) 70, 80, 341, 429, 727
HincII GTYRAC 2 cut(s) 174, 794
HindII GTYRAC 2 cut(s) 174, 794
HinfI GANTC 3 cut(s) 820, 946, 1363
HpaII CCGG 3 cut(s) 281, 373, 779
HphI GGTGA 2 cut(s) 127, 154
Hpy166II GTNNAC 4 cut(s) 174, 794, 848, 1092
Hpy188I TCNGA 6 cut(s) 323, 537, 804, 951, 1005, 1455
Hpy188III TCNNGA 7 cut(s) 241, 281, 353, 505, 779, 812, 1367
Hpy8I GTNNAC 4 cut(s) 174, 794, 848, 1092
Hpy99I CGWCG 1 cut(s) 845
HpyAV CCTTC 5 cut(s) 589, 893, 1148, 1172, 1460
HpyCH4III ACNGT 6 cut(s) 38, 332, 614, 845, 908, 1351
HpyCH4IV ACGT 1 cut(s) 539
HpyCH4V TGCA 5 cut(s) 31, 370, 518, 679, 873
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 125
HpyF3I CTNAG 7 cut(s) 320, 351, 553, 948, 1129, 1202, 1426
HpySE526I ACGT 1 cut(s) 539
Hsp92II CATG 5 cut(s) 70, 80, 341, 429, 727
Kpn2I TCCGGA 2 cut(s) 280, 778
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 4 cut(s) 142, 156, 532, 655
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 2 cut(s) 283, 1208
Lsp1109I GCAGC 5 cut(s) 15, 83, 202, 1275, 1308
LweI GCATC 1 cut(s) 1432
MaeI CTAG 6 cut(s) 287, 363, 1187, 1259, 1290, 1418
MaeII ACGT 1 cut(s) 539
MaeIII GTNAC 5 cut(s) 61, 191, 205, 557, 827
MalI GATC 4 cut(s) 144, 158, 534, 657
MboI GATC 4 cut(s) 142, 156, 532, 655
MfeI CAATTG 4 cut(s) 659, 680, 887, 960
MlyI GAGTC 3 cut(s) 814, 940, 1357
MroI TCCGGA 2 cut(s) 280, 778
MroXI GAANNNNTTC 3 cut(s) 295, 882, 1052
MseI TTAA 4 cut(s) 459, 692, 764, 1076
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 1321
MspI CCGG 3 cut(s) 281, 373, 779
MspR9I CCNGG 2 cut(s) 608, 1244
MunI CAATTG 4 cut(s) 659, 680, 887, 960
Mva1269I GAATGC 2 cut(s) 691, 875
MvaI CCWGG 2 cut(s) 608, 1244
MwoI GCNNNNNNNGC 2 cut(s) 116, 125
NcoI CCATGG 1 cut(s) 66
NdeII GATC 4 cut(s) 142, 156, 532, 655
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 5 cut(s) 70, 80, 341, 429, 727
NlaIV GGNNCC 5 cut(s) 271, 343, 415, 436, 487
NspI RCATGY 2 cut(s) 80, 727
PceI AGGCCT 1 cut(s) 1155
PciI ACATGT 2 cut(s) 76, 723
PciSI GCTCTTC 1 cut(s) 117
PctI GAATGC 2 cut(s) 691, 875
PdmI GAANNNNTTC 3 cut(s) 295, 882, 1052
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 6 cut(s) 30, 52, 73, 217, 1265, 1323
PleI GAGTC 3 cut(s) 814, 940, 1357
PpsI GAGTC 3 cut(s) 814, 940, 1357
PpuMI RGGWCCY 2 cut(s) 435, 989
PscI ACATGT 2 cut(s) 76, 723
Psp5II RGGWCCY 2 cut(s) 435, 989
Psp6I CCWGG 2 cut(s) 606, 1242
PspGI CCWGG 2 cut(s) 606, 1242
PspN4I GGNNCC 5 cut(s) 271, 343, 415, 436, 487
PspPI GGNCC 3 cut(s) 435, 845, 989
PspPPI RGGWCCY 2 cut(s) 435, 989
PstI CTGCAG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1321
RsaI GTAC 6 cut(s) 35, 81, 343, 897, 1303, 1357
RsaNI GTAC 6 cut(s) 34, 80, 342, 896, 1302, 1356
RseI CAYNNNNRTG 1 cut(s) 587
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 4 cut(s) 459, 692, 764, 1076
SatI GCNGC 6 cut(s) 29, 51, 72, 216, 1264, 1322
Sau3AI GATC 4 cut(s) 142, 156, 532, 655
Sau96I GGNCC 3 cut(s) 435, 845, 989
ScaI AGTACT 2 cut(s) 35, 1357
SchI GAGTC 3 cut(s) 814, 940, 1357
ScrFI CCNGG 2 cut(s) 608, 1244
SfaNI GCATC 1 cut(s) 1432
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 3 cut(s) 435, 845, 989
SmiI ATTTAAAT 1 cut(s) 765
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 1408
SmoI CTYRAG 1 cut(s) 1408
SseBI AGGCCT 1 cut(s) 1155
SsiI CCGC 3 cut(s) 50, 236, 1206
SspI AATATT 1 cut(s) 669
SspMI CTAG 6 cut(s) 287, 363, 1187, 1259, 1290, 1418
StuI AGGCCT 1 cut(s) 1155
StyD4I CCNGG 2 cut(s) 606, 1242
StyI CCWWGG 4 cut(s) 66, 967, 1156, 1258
SwaI ATTTAAAT 1 cut(s) 765
TaaI ACNGT 6 cut(s) 38, 332, 614, 845, 908, 1351
TaiI ACGT 1 cut(s) 542
TaqI TCGA 4 cut(s) 840, 857, 1116, 1361
TatI WGTACW 3 cut(s) 33, 79, 1355
TauI GCSGC 1 cut(s) 53
Tru1I TTAA 4 cut(s) 459, 692, 764, 1076
Tru9I TTAA 4 cut(s) 459, 692, 764, 1076
TscAI CASTG 3 cut(s) 271, 919, 1107
TseI GCWGC 5 cut(s) 28, 71, 215, 1263, 1321
TspDTI ATGAA 2 cut(s) 288, 326
TspRI CASTG 3 cut(s) 271, 919, 1107
VpaK11BI GGWCC 3 cut(s) 435, 845, 989
XapI RAATTY 3 cut(s) 620, 630, 1021
XceI RCATGY 2 cut(s) 80, 727
XcmI CCANNNNNNNNNTGG 2 cut(s) 192, 861
XmaJI CCTAGG 1 cut(s) 1258
XmnI GAANNNNTTC 3 cut(s) 295, 882, 1052
XspI CTAG 6 cut(s) 287, 363, 1187, 1259, 1290, 1418
ZrmI AGTACT 2 cut(s) 35, 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.