Rh6DG218000

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0030359
Physical Location & Seq
Forward (+)
38831024 .. 38838317
7294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG218000.1

Sequence Viewer

Length: 1230 bp
ATGGAGAGAATTGCTTGGTTCTTCCTGCTGCAGTACTGTTGTTGTATGGCGGCTACCTGCGTAACCATGGCAGCACATGTACAAACCAACATCAGCATAGACCAGTCTGCTCTTCTTGCTTTCAAAGCCAGTATCACCGATGATCCTCATAAACTGATCTTCACCAACTGGTCAACCATCGTCCCAGTTTGTAACTGGGTTGGCGTTACTTGTGCTGCTCGCCACCACAGAGTTGCGGTCTTGAACTTATCTTATTTTGGTCTCACTGGCACCATTCCTCCGGAGCTAGGAAACCTTTCATTTCTTGTGGAACTTCGCCTCAGAAATAACAGTTTTCATGGTACCTTGCCTCAGGAATTGGCTAGCCTGCACCGGTTGAGGTTGATTAGCTTGGGATATAACAAGTTTATGGGAACCATTCCCTCATGGTTCGGGTCCTTATCCAAACTTCAAACATTTAATTTGTATGGTAATCAGTTTTCGGGTTCCATACCAGCAACCATCTTGAACTTATCTGCACTTCAAGTAATTGATCTGACGTATAACCAACTCTCAGGTAACATACCAAGAGAGATTGGAAACATAACAATGTTGAAGGAAATATACCTGGACAGTAATAAATTCACAGAAATTCCTAATGAGATTGGCAGTTTAGATCAATTGGAAATATTGTTTGTGCAATTGAATGCTTTAAAAGGGTCTATTCCTATGGTTGTTTTCAACATGTCTTCTTTGACTATGTTGGCTCTATATGGAAACAATTTAAATGGTAGTCTTCCGGACAATATATGTCAACATCTTCCGAGTATTCAAGATTTGACTCTGGGTTACAACCAATTCGACGGTCCACTTCCATCGAAATTGTGGGAATGCAAAGAACTCCTTCAATTGGAAGTACCACGCAACAGTTTCAGTGGAGAAATACCAAATACTTTTGGGAACTTGACTCAGATACAGACAATTGACCTTGGCTTGAACTATTTGACAGGTAGAGGTCCAAGAGCTTCCTCCCTAGACCACGACAATGCTGGAGGATCGCGGCCAGCCGCTAGACTGGTGACCGGAGGCCGTTCCCTCAAGCGGGTCTGGGGAGGTGAGAAGCAGGAGATGGGCTCTAGCTTTGGGCCTCTGCCCACCTGGGCTGCTGCTTATGGCCTGGCTAGGCTAGCCCACTCTCTTTCCACTTTCCTTTGTTGGATTAGGCCTGTTTGTGGGCTTTCGGGGGAGTGA

Protein Analysis

409

Amino Acids

45.02

Weight (kDa)

7.09

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 71 1.1e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 181 1.5e-07 Leucine-rich repeat region
LRR_8 PF13855 125 - 184 6.4e-08 Leucine rich repeat
LRR_14 PF23598 168 - 275 1.2e-09 Leucine-rich repeat region
LRR_8 PF13855 198 - 255 1e-05 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030359)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0277691
rosa_samantha Rh6DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 269, 341
AccII CGCG 1 cut(s) 1039
AccIII TCCGGA 2 cut(s) 280, 778
AciI CCGC 5 cut(s) 50, 236, 1039, 1047, 1081
AclWI GGATC 2 cut(s) 137, 1042
AcoI YGGCCR 1 cut(s) 1040
AcsI RAATTY 2 cut(s) 620, 630
AfaI GTAC 4 cut(s) 35, 81, 343, 897
AfiI CCNNNNNNNGG 5 cut(s) 287, 889, 1080, 1081, 1211
AflIII ACRYGT 2 cut(s) 76, 723
AgeI ACCGGT 1 cut(s) 372
AjnI CCWGG 3 cut(s) 606, 1136, 1155
AluBI AGCT 4 cut(s) 286, 390, 1004, 1119
AluI AGCT 4 cut(s) 286, 390, 1004, 1119
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 2 cut(s) 137, 1042
Aor13HI TCCGGA 2 cut(s) 280, 778
AoxI GGCC 5 cut(s) 1040, 1066, 1124, 1153, 1202
ApeKI GCWGC 5 cut(s) 28, 71, 215, 1142, 1145
ApoI RAATTY 2 cut(s) 620, 630
AsiGI ACCGGT 1 cut(s) 372
Asp700I GAANNNNTTC 2 cut(s) 295, 882
Asp718I GGTACC 1 cut(s) 341
AspS9I GGNCC 4 cut(s) 435, 845, 995, 1124
AsuHPI GGTGA 4 cut(s) 127, 154, 1069, 1106
AsuNHI GCTAGC 2 cut(s) 362, 1165
AvaII GGWCC 3 cut(s) 435, 845, 995
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 2 cut(s) 269, 341
BanII GRGCYC 1 cut(s) 1115
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BbsI GAAGAC 2 cut(s) 720, 767
BbvI GCAGC 5 cut(s) 15, 83, 202, 1129, 1132
BccI CCATC 4 cut(s) 185, 509, 862, 1102
BceAI ACGGC 1 cut(s) 1053
BciT130I CCWGG 3 cut(s) 608, 1138, 1157
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 7 cut(s) 287, 363, 1013, 1050, 1116, 1161, 1166
BfmI CTRYAG 1 cut(s) 29
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 8 cut(s) 29, 51, 72, 216, 1040, 1047, 1143, 1146
BlsI GCNGC 8 cut(s) 30, 52, 73, 217, 1041, 1048, 1144, 1147
BmcAI AGTACT 1 cut(s) 35
Bme1390I CCNGG 3 cut(s) 608, 1138, 1157
Bme18I GGWCC 3 cut(s) 435, 845, 995
BmgT120I GGNCC 4 cut(s) 435, 845, 995, 1124
BmiI GGNNCC 5 cut(s) 271, 343, 415, 436, 487
BmrFI CCNGG 3 cut(s) 608, 1138, 1157
BmrI ACTGGG 2 cut(s) 179, 205
BmtI GCTAGC 2 cut(s) 366, 1169
BmuI ACTGGG 2 cut(s) 179, 205
BpiI GAAGAC 2 cut(s) 720, 767
BplI GAGNNNNNCTC 2 cut(s) 1097, 1129
BpmI CTGGAG 1 cut(s) 1050
BpuEI CTTGAG 1 cut(s) 1061
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 3 cut(s) 66, 967, 1137
BsaWI WCCGGW 4 cut(s) 280, 372, 778, 1061
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 5 cut(s) 287, 889, 1080, 1081, 1211
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 7 cut(s) 103, 129, 173, 185, 200, 271, 1059
Bse21I CCTNAGG 1 cut(s) 351
BseAI TCCGGA 2 cut(s) 280, 778
BseBI CCWGG 3 cut(s) 608, 1138, 1157
BseDI CCNNGG 3 cut(s) 66, 967, 1137
BseLI CCNNNNNNNGG 5 cut(s) 287, 889, 1080, 1081, 1211
BseMII CTCAG 4 cut(s) 334, 365, 567, 962
BseNI ACTGG 7 cut(s) 103, 129, 173, 185, 200, 271, 1059
BseXI GCAGC 5 cut(s) 15, 83, 202, 1129, 1132
BsgI GTGCAG 2 cut(s) 353, 501
Bsh1236I CGCG 1 cut(s) 1039
BshFI GGCC 5 cut(s) 1042, 1068, 1126, 1155, 1204
BshNI GGYRCC 2 cut(s) 269, 341
BshTI ACCGGT 1 cut(s) 372
BsiSI CCGG 4 cut(s) 281, 373, 779, 1062
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 5 cut(s) 287, 889, 1080, 1081, 1211
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 691, 875
BsnI GGCC 5 cut(s) 1042, 1068, 1126, 1155, 1204
Bso31I GGTCTC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 1115
Bsp13I TCCGGA 2 cut(s) 280, 778
Bsp1407I TGTACA 1 cut(s) 79
Bsp143I GATC 5 cut(s) 142, 156, 532, 655, 1034
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 5 cut(s) 50, 236, 1039, 1047, 1081
BspANI GGCC 5 cut(s) 1042, 1068, 1126, 1155, 1204
BspCNI CTCAG 4 cut(s) 333, 364, 566, 961
BspEI TCCGGA 2 cut(s) 280, 778
BspFNI CGCG 1 cut(s) 1039
BspLI GGNNCC 5 cut(s) 271, 343, 415, 436, 487
BspMAI CTGCAG 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 65
BspOI GCTAGC 2 cut(s) 366, 1169
BspPI GGATC 2 cut(s) 137, 1042
BspQI GCTCTTC 1 cut(s) 117
BspT107I GGYRCC 2 cut(s) 269, 341
BspTNI GGTCTC 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 372
BsrGI TGTACA 1 cut(s) 79
BsrI ACTGG 7 cut(s) 103, 129, 173, 185, 200, 271, 1059
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 3 cut(s) 66, 967, 1137
BssMI GATC 5 cut(s) 142, 156, 532, 655, 1034
BssT1I CCWWGG 2 cut(s) 66, 967
Bst2UI CCWGG 3 cut(s) 608, 1138, 1157
Bst4CI ACNGT 5 cut(s) 38, 332, 614, 845, 908
Bst6I CTCTTC 1 cut(s) 117
BstAUI TGTACA 1 cut(s) 79
BstC8I GCNNGC 5 cut(s) 220, 364, 368, 1044, 1167
BstDEI CTNAG 4 cut(s) 320, 351, 553, 948
BstDSI CCRYGG 1 cut(s) 66
BstEII GGTNACC 1 cut(s) 1057
BstFNI CGCG 1 cut(s) 1039
BstKTI GATC 5 cut(s) 145, 159, 535, 658, 1037
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 5 cut(s) 142, 156, 532, 655, 1034
BstMWI GCNNNNNNNGC 3 cut(s) 116, 125, 1166
BstNI CCWGG 3 cut(s) 608, 1138, 1157
BstNSI RCATGY 2 cut(s) 80, 727
BstPI GGTNACC 1 cut(s) 1057
BstSCI CCNGG 3 cut(s) 606, 1136, 1155
BstSFI CTRYAG 1 cut(s) 29
BstUI CGCG 1 cut(s) 1039
BstV1I GCAGC 5 cut(s) 15, 83, 202, 1129, 1132
BstV2I GAAGAC 2 cut(s) 720, 767
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 5 cut(s) 1042, 1068, 1126, 1155, 1204
BtgI CCRYGG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 264, 919
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 5 cut(s) 220, 364, 368, 1044, 1167
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 4 cut(s) 435, 845, 995, 1124
Csp6I GTAC 4 cut(s) 34, 80, 342, 896
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 5 cut(s) 67, 77, 338, 426, 724
CviQI GTAC 4 cut(s) 34, 80, 342, 896
DdeI CTNAG 4 cut(s) 320, 351, 553, 948
DpnI GATC 5 cut(s) 144, 158, 534, 657, 1036
DpnII GATC 5 cut(s) 142, 156, 532, 655, 1034
DraI TTTAAA 2 cut(s) 693, 765
EaeI YGGCCR 1 cut(s) 1040
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 66, 967
Eco147I AGGCCT 1 cut(s) 1204
Eco24I GRGCYC 1 cut(s) 1115
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 3 cut(s) 435, 845, 995
Eco81I CCTNAGG 1 cut(s) 351
Eco91I GGTNACC 1 cut(s) 1057
EcoO109I RGGNCCY 1 cut(s) 435
EcoO65I GGTNACC 1 cut(s) 1057
EcoRII CCWGG 3 cut(s) 606, 1136, 1155
EcoT14I CCWWGG 2 cut(s) 66, 967
EcoT38I GRGCYC 1 cut(s) 1115
ErhI CCWWGG 2 cut(s) 66, 967
FaeI CATG 5 cut(s) 70, 80, 341, 429, 727
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 1 cut(s) 167
FatI CATG 5 cut(s) 66, 76, 337, 425, 723
FauI CCCGC 1 cut(s) 1074
Fnu4HI GCNGC 8 cut(s) 29, 51, 72, 216, 1040, 1047, 1143, 1146
FriOI GRGCYC 1 cut(s) 1115
Fsp4HI GCNGC 8 cut(s) 29, 51, 72, 216, 1040, 1047, 1143, 1146
FspBI CTAG 7 cut(s) 287, 363, 1013, 1050, 1116, 1161, 1166
GluI GCNGC 8 cut(s) 29, 51, 72, 216, 1040, 1047, 1143, 1146
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 5 cut(s) 1042, 1068, 1126, 1155, 1204
HapII CCGG 4 cut(s) 281, 373, 779, 1062
Hin1II CATG 5 cut(s) 70, 80, 341, 429, 727
HincII GTYRAC 2 cut(s) 174, 794
HindII GTYRAC 2 cut(s) 174, 794
HinfI GANTC 2 cut(s) 820, 946
HpaII CCGG 4 cut(s) 281, 373, 779, 1062
HphI GGTGA 4 cut(s) 127, 154, 1069, 1106
Hpy166II GTNNAC 3 cut(s) 174, 794, 848
Hpy188I TCNGA 4 cut(s) 323, 537, 804, 951
Hpy188III TCNNGA 6 cut(s) 241, 281, 353, 505, 779, 812
Hpy8I GTNNAC 3 cut(s) 174, 794, 848
Hpy99I CGWCG 1 cut(s) 845
HpyAV CCTTC 2 cut(s) 589, 893
HpyCH4III ACNGT 5 cut(s) 38, 332, 614, 845, 908
HpyCH4IV ACGT 1 cut(s) 539
HpyCH4V TGCA 5 cut(s) 31, 370, 518, 679, 873
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 125, 1166
HpyF3I CTNAG 4 cut(s) 320, 351, 553, 948
HpySE526I ACGT 1 cut(s) 539
Hsp92II CATG 5 cut(s) 70, 80, 341, 429, 727
Kpn2I TCCGGA 2 cut(s) 280, 778
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 5 cut(s) 142, 156, 532, 655, 1034
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 5 cut(s) 15, 83, 202, 1129, 1132
MaeI CTAG 7 cut(s) 287, 363, 1013, 1050, 1116, 1161, 1166
MaeII ACGT 1 cut(s) 539
MaeIII GTNAC 6 cut(s) 61, 191, 205, 557, 827, 1057
MalI GATC 5 cut(s) 144, 158, 534, 657, 1036
MboI GATC 5 cut(s) 142, 156, 532, 655, 1034
MboII GAAGA 6 cut(s) 13, 104, 151, 720, 767, 791
MfeI CAATTG 4 cut(s) 659, 680, 887, 960
MhlI GDGCHC 1 cut(s) 1115
MlyI GAGTC 2 cut(s) 814, 940
MmeI TCCRAC 1 cut(s) 1175
MroI TCCGGA 2 cut(s) 280, 778
MroXI GAANNNNTTC 2 cut(s) 295, 882
MseI TTAA 3 cut(s) 459, 692, 764
MslI CAYNNNNRTG 2 cut(s) 587, 1023
MspI CCGG 4 cut(s) 281, 373, 779, 1062
MspR9I CCNGG 3 cut(s) 608, 1138, 1157
MunI CAATTG 4 cut(s) 659, 680, 887, 960
Mva1269I GAATGC 2 cut(s) 691, 875
MvaI CCWGG 3 cut(s) 608, 1138, 1157
MvnI CGCG 1 cut(s) 1039
MwoI GCNNNNNNNGC 3 cut(s) 116, 125, 1166
NcoI CCATGG 1 cut(s) 66
NdeII GATC 5 cut(s) 142, 156, 532, 655, 1034
NheI GCTAGC 2 cut(s) 362, 1165
NlaIII CATG 5 cut(s) 70, 80, 341, 429, 727
NlaIV GGNNCC 5 cut(s) 271, 343, 415, 436, 487
NmuCI GTSAC 1 cut(s) 1057
NspI RCATGY 2 cut(s) 80, 727
PceI AGGCCT 1 cut(s) 1204
PciI ACATGT 2 cut(s) 76, 723
PciSI GCTCTTC 1 cut(s) 117
PctI GAATGC 2 cut(s) 691, 875
PdmI GAANNNNTTC 2 cut(s) 295, 882
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 8 cut(s) 30, 52, 73, 217, 1041, 1048, 1144, 1147
PleI GAGTC 2 cut(s) 814, 940
PpsI GAGTC 2 cut(s) 814, 940
PpuMI RGGWCCY 1 cut(s) 435
PscI ACATGT 2 cut(s) 76, 723
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 606, 1136, 1155
PspEI GGTNACC 1 cut(s) 1057
PspGI CCWGG 3 cut(s) 606, 1136, 1155
PspN4I GGNNCC 5 cut(s) 271, 343, 415, 436, 487
PspPI GGNCC 4 cut(s) 435, 845, 995, 1124
PspPPI RGGWCCY 1 cut(s) 435
PstI CTGCAG 1 cut(s) 33
RsaI GTAC 4 cut(s) 35, 81, 343, 897
RsaNI GTAC 4 cut(s) 34, 80, 342, 896
RseI CAYNNNNRTG 2 cut(s) 587, 1023
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 459, 692, 764
SatI GCNGC 8 cut(s) 29, 51, 72, 216, 1040, 1047, 1143, 1146
Sau3AI GATC 5 cut(s) 142, 156, 532, 655, 1034
Sau96I GGNCC 4 cut(s) 435, 845, 995, 1124
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 2 cut(s) 814, 940
ScrFI CCNGG 3 cut(s) 608, 1138, 1157
SduI GDGCHC 1 cut(s) 1115
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 3 cut(s) 435, 845, 995
SmiI ATTTAAAT 1 cut(s) 765
SmiMI CAYNNNNRTG 2 cut(s) 587, 1023
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
SseBI AGGCCT 1 cut(s) 1204
SsiI CCGC 5 cut(s) 50, 236, 1039, 1047, 1081
SspI AATATT 1 cut(s) 669
SspMI CTAG 7 cut(s) 287, 363, 1013, 1050, 1116, 1161, 1166
StuI AGGCCT 1 cut(s) 1204
StyD4I CCNGG 3 cut(s) 606, 1136, 1155
StyI CCWWGG 2 cut(s) 66, 967
SwaI ATTTAAAT 1 cut(s) 765
TaaI ACNGT 5 cut(s) 38, 332, 614, 845, 908
TaiI ACGT 1 cut(s) 542
TaqI TCGA 2 cut(s) 840, 857
TatI WGTACW 2 cut(s) 33, 79
TauI GCSGC 3 cut(s) 53, 1042, 1049
Tru1I TTAA 3 cut(s) 459, 692, 764
Tru9I TTAA 3 cut(s) 459, 692, 764
TscAI CASTG 2 cut(s) 271, 919
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 5 cut(s) 28, 71, 215, 1142, 1145
Tsp45I GTSAC 1 cut(s) 1057
TspDTI ATGAA 2 cut(s) 288, 326
TspRI CASTG 2 cut(s) 271, 919
VpaK11BI GGWCC 3 cut(s) 435, 845, 995
XapI RAATTY 2 cut(s) 620, 630
XceI RCATGY 2 cut(s) 80, 727
XcmI CCANNNNNNNNNTGG 3 cut(s) 192, 861, 1025
XmnI GAANNNNTTC 2 cut(s) 295, 882
XspI CTAG 7 cut(s) 287, 363, 1013, 1050, 1116, 1161, 1166
ZrmI AGTACT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.