RchiOBHm_Chr6g0283341

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025064
Physical Location & Seq
Reverse (-)
46558310 .. 46559203
894 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25404

Sequence Viewer

Length: 582 bp
ATGGAATCGCTACAAAGCCGAGTGGAGTCATGGCTGAGGGGTTGGGAGAAGCGAGCCAAGCTCTGGAAGGTGTCCGGGGGCCCACTCCAGTGGAGGATGAGGTGGCCTCGGATTCTCAACGACGGCTACAGGGAGCAGCGGAAGAGGATCCACGAGGAGTACGAGCGTCGCCGGAAGCAGCTCCACGATCTCTGCGCCGCCGTCAAGGCTGACTCCATCTCCGACTTGCAGGACATATTCTGCTGCATGGTGCTTTCAGAGTGCGTCTATAAGATACCTGCTTCGGACTTGGTTCGCGCGGTGAACAGATTCAAGGCTGACTTTGGAGGACAGATTGTTGCGCTGGAGCGTGATCAGCCTTTTTCTGATCACGTTCCTCACAGTTACTTGTTGGCGGAAGCAGGTGATACATTGTTTGCTTCCTTCATTAGAACTAAGCAGTACAACGATGTGATGACCGATGCAAACATACTGCAAGGTGCTATATTTCATGAGGATCCTGTGGAGGATACTGATGGGACTAATCCACCTGGAAGTCGTAAGGGAAATGGAGGATACTGCTACTTCTACCTCCGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

193

Amino Acids

22.39

Weight (kDa)

7.65

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025064)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0283341
rosa_multiflora Rmu_sc0013038.1_g000005
rosa_samantha Rh6DG261400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 392
Acc36I ACCTGC 2 cut(s) 286, 392
AccB7I CCANNNNNTGG 1 cut(s) 63
AccII CGCG 2 cut(s) 297, 299
AciI CCGC 4 cut(s) 139, 198, 299, 395
AclWI GGATC 4 cut(s) 142, 155, 491, 504
AfaI GTAC 2 cut(s) 161, 443
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 1 cut(s) 313
AjnI CCWGG 1 cut(s) 529
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 2 cut(s) 61, 181
AluI AGCT 2 cut(s) 61, 181
AlwI GGATC 4 cut(s) 142, 155, 491, 504
AoxI GGCC 2 cut(s) 79, 104
ApaI GGGCCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 136, 178, 243
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 3 cut(s) 197, 299, 343
AspS9I GGNCC 2 cut(s) 79, 80
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 313, 416
BaeGI GKGCMC 1 cut(s) 83
BamHI GGATCC 2 cut(s) 147, 496
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 152
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 148, 190, 230
BccI CCATC 2 cut(s) 224, 509
BceAI ACGGC 2 cut(s) 139, 185
BciT130I CCWGG 1 cut(s) 531
BciVI GTATCC 2 cut(s) 502, 548
BclI TGATCA 2 cut(s) 352, 367
BcnI CCSGG 1 cut(s) 76
BfmI CTRYAG 1 cut(s) 127
BfuAI ACCTGC 2 cut(s) 286, 392
BfuI GTATCC 2 cut(s) 502, 548
BglI GCCNNNNNGGC 1 cut(s) 206
BisI GCNGC 4 cut(s) 137, 179, 198, 244
BlsI GCNGC 4 cut(s) 138, 180, 199, 245
Bme1390I CCNGG 2 cut(s) 76, 531
BmgT120I GGNCC 2 cut(s) 79, 80
BmiI GGNNCC 4 cut(s) 80, 81, 149, 498
BmrFI CCNGG 2 cut(s) 76, 531
BmsI GCATC 1 cut(s) 451
BplI GAGNNNNNCTC 4 cut(s) 45, 77, 91, 123
BpmI CTGGAG 2 cut(s) 71, 365
Bpu10I CCTNAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 75, 107
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 88
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 75, 107
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 170
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 3 cut(s) 148, 190, 230
Bsh1236I CGCG 2 cut(s) 297, 299
BshFI GGCC 2 cut(s) 81, 106
BsiSI CCGG 2 cut(s) 75, 172
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 2 cut(s) 81, 106
Bsp120I GGGCCC 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 5 cut(s) 147, 187, 352, 367, 496
BspACI CCGC 4 cut(s) 139, 198, 299, 395
BspANI GGCC 2 cut(s) 81, 106
BspCNI CTCAG 1 cut(s) 27
BspFNI CGCG 2 cut(s) 297, 299
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 4 cut(s) 80, 81, 149, 498
BspMI ACCTGC 2 cut(s) 286, 392
BspPI GGATC 4 cut(s) 142, 155, 491, 504
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 2 cut(s) 75, 107
BssMI GATC 5 cut(s) 147, 187, 352, 367, 496
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 2 cut(s) 35, 435
BstF5I GGATG 1 cut(s) 102
BstFNI CGCG 2 cut(s) 297, 299
BstHHI GCGC 3 cut(s) 197, 299, 343
BstKTI GATC 5 cut(s) 150, 190, 355, 370, 499
BstMBI GATC 5 cut(s) 147, 187, 352, 367, 496
BstMWI GCNNNNNNNGC 3 cut(s) 58, 206, 355
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 2 cut(s) 74, 529
BstSFI CTRYAG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 83
BstUI CGCG 2 cut(s) 297, 299
BstV1I GCAGC 3 cut(s) 148, 190, 230
BstX2I RGATCY 2 cut(s) 147, 496
BstXI CCANNNNNNTGG 1 cut(s) 90
BstYI RGATCY 2 cut(s) 147, 496
BsuI GTATCC 2 cut(s) 502, 548
BsuRI GGCC 2 cut(s) 81, 106
BtsCI GGATG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 95
BveI ACCTGC 2 cut(s) 286, 392
Cac8I GCNNGC 1 cut(s) 54
CciI TCATGA 1 cut(s) 490
CfoI GCGC 3 cut(s) 197, 299, 343
Cfr13I GGNCC 2 cut(s) 79, 80
CseI GACGC 2 cut(s) 155, 253
Csp6I GTAC 2 cut(s) 160, 442
CviAII CATG 3 cut(s) 30, 247, 491
CviQI GTAC 2 cut(s) 160, 442
DdeI CTNAG 2 cut(s) 35, 435
DpnI GATC 5 cut(s) 149, 189, 354, 369, 498
DpnII GATC 5 cut(s) 147, 187, 352, 367, 496
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EciI GGCGGA 1 cut(s) 410
Eco24I GRGCYC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 83
FaeI CATG 3 cut(s) 33, 250, 494
FaiI YATR 8 cut(s) 31, 236, 248, 270, 470, 485, 492, 580
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 1 cut(s) 532
FatI CATG 3 cut(s) 29, 246, 490
FbaI TGATCA 2 cut(s) 352, 367
Fnu4HI GCNGC 4 cut(s) 137, 179, 198, 244
FokI GGATG 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 137, 179, 198, 244
GlaI GCGC 3 cut(s) 196, 298, 342
GluI GCNGC 4 cut(s) 137, 179, 198, 244
GsuI CTGGAG 2 cut(s) 71, 365
HaeIII GGCC 2 cut(s) 81, 106
HapII CCGG 2 cut(s) 75, 172
HgaI GACGC 2 cut(s) 155, 253
HhaI GCGC 3 cut(s) 197, 299, 343
Hin1II CATG 3 cut(s) 33, 250, 494
Hin6I GCGC 3 cut(s) 195, 297, 341
HinP1I GCGC 3 cut(s) 195, 297, 341
HinfI GANTC 5 cut(s) 5, 26, 112, 212, 309
HpaII CCGG 2 cut(s) 75, 172
HphI GGTGA 2 cut(s) 313, 416
Hpy166II GTNNAC 1 cut(s) 304
Hpy188I TCNGA 6 cut(s) 111, 223, 259, 286, 367, 575
Hpy188III TCNNGA 2 cut(s) 64, 491
Hpy8I GTNNAC 1 cut(s) 304
Hpy99I CGWCG 2 cut(s) 125, 171
HpyAV CCTTC 2 cut(s) 61, 433
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 229, 246, 464, 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 206, 355
HpyF3I CTNAG 2 cut(s) 35, 435
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 3 cut(s) 33, 250, 494
HspAI GCGC 3 cut(s) 195, 297, 341
Ksp22I TGATCA 2 cut(s) 352, 367
Kzo9I GATC 5 cut(s) 147, 187, 352, 367, 496
LmnI GCTCC 3 cut(s) 133, 186, 346
Lsp1109I GCAGC 3 cut(s) 148, 190, 230
LweI GCATC 1 cut(s) 451
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 383
MalI GATC 5 cut(s) 149, 189, 354, 369, 498
MboI GATC 5 cut(s) 147, 187, 352, 367, 496
MboII GAAGA 1 cut(s) 154
MflI RGATCY 2 cut(s) 147, 496
MhlI GDGCHC 1 cut(s) 83
MlyI GAGTC 2 cut(s) 35, 206
MmeI TCCRAC 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 308
MslI CAYNNNNRTG 1 cut(s) 88
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 2 cut(s) 75, 172
MspR9I CCNGG 2 cut(s) 76, 531
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 2 cut(s) 297, 299
MwoI GCNNNNNNNGC 3 cut(s) 58, 206, 355
NciI CCSGG 1 cut(s) 76
NdeII GATC 5 cut(s) 147, 187, 352, 367, 496
NlaIII CATG 3 cut(s) 33, 250, 494
NlaIV GGNNCC 4 cut(s) 80, 81, 149, 498
NmeAIII GCCGAG 1 cut(s) 44
OliI CACNNNNGTG 1 cut(s) 88
PagI TCATGA 1 cut(s) 490
PaqCI CACCTGC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 3 cut(s) 5, 112, 309
PflMI CCANNNNNTGG 1 cut(s) 63
PkrI GCNGC 4 cut(s) 138, 180, 199, 245
PleI GAGTC 2 cut(s) 34, 206
PpsI GAGTC 2 cut(s) 34, 206
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 4 cut(s) 80, 81, 149, 498
PspOMI GGGCCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 79, 80
PsuI RGATCY 2 cut(s) 147, 496
RsaI GTAC 2 cut(s) 161, 443
RsaNI GTAC 2 cut(s) 160, 442
RseI CAYNNNNRTG 1 cut(s) 88
SatI GCNGC 4 cut(s) 137, 179, 198, 244
Sau3AI GATC 5 cut(s) 147, 187, 352, 367, 496
Sau96I GGNCC 2 cut(s) 79, 80
SchI GAGTC 2 cut(s) 35, 206
ScrFI CCNGG 2 cut(s) 76, 531
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 1 cut(s) 451
SfcI CTRYAG 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 88
SsiI CCGC 4 cut(s) 139, 198, 299, 395
StyD4I CCNGG 2 cut(s) 74, 529
TaaI ACNGT 1 cut(s) 383
TaiI ACGT 1 cut(s) 375
TaqII GACCGA 1 cut(s) 473
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 3 cut(s) 5, 112, 309
TscAI CASTG 1 cut(s) 95
TseI GCWGC 3 cut(s) 136, 178, 243
TspDTI ATGAA 2 cut(s) 415, 479
TspRI CASTG 1 cut(s) 95
Van91I CCANNNNNTGG 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.