RchiOBHm_Chr7g0195251

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0025149
Physical Location & Seq
Reverse (-)
13147320 .. 13147785
466 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17460

Sequence Viewer

Length: 342 bp
ATGGAGAAGAACAAAGTTTCTAGAGTTTGTGTTACAGGTGGTTCTGGATATAACGGCTGTTGGCTTGTCAAAAAGCTCCTGGAGAATGGCCATACAGTCCATCCCACTGATGCATCAAAAGTAGGCCTTCTGAAGTCTCTCCCTGATGCAGACTCCAAGTTATTGCTGTTCCAAGCTGATTTATATGATCCTAATGAGTTTGAGCATGCTATTCAAGGGAGTCAGTTTGTTTTTCATGTTGCCACTCCTACTGAACATAACCCTGAAATCTCCAAGGTCAGTCTCTTAATTAGTACCAAGTCCCTAATTAGTTGTTGTCTTGTTGATCTGTCTTATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

113

Amino Acids

12.44

Weight (kDa)

6.17

Isoelectric Point (pI)

20.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 92 1.2e-06 NmrA-like family
Epimerase PF01370 9 - 88 7.4e-09 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 106 6.1e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 81 1.1e-06 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025149)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0195251
rosa_roxburghii Rroxscaffold_3G00260640
rosa_samantha Rh7DG150300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 295
AgsI TTSAA 1 cut(s) 215
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 2 cut(s) 76, 176
AluI AGCT 2 cut(s) 76, 176
Alw26I GTCTC 2 cut(s) 141, 287
AlwI GGATC 1 cut(s) 182
AoxI GGCC 2 cut(s) 88, 124
BalI TGGCCA 1 cut(s) 90
BccI CCATC 1 cut(s) 108
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 141, 287
BfaI CTAG 1 cut(s) 21
Bme1390I CCNGG 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 3 cut(s) 100, 122, 136
BpmI CTGGAG 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 100
BshFI GGCC 2 cut(s) 90, 126
BslFI GGGAC 1 cut(s) 286
BsmAI GTCTC 2 cut(s) 141, 287
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 2 cut(s) 90, 126
Bsp143I GATC 2 cut(s) 187, 325
BspANI GGCC 2 cut(s) 90, 126
BspPI GGATC 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 2 cut(s) 187, 325
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 207
BstF5I GGATG 1 cut(s) 100
BstKTI GATC 2 cut(s) 190, 328
BstMAI GTCTC 2 cut(s) 141, 287
BstMBI GATC 2 cut(s) 187, 325
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 1 cut(s) 78
BsuRI GGCC 2 cut(s) 90, 126
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 294
CviAII CATG 2 cut(s) 206, 236
CviJI RGCY 6 cut(s) 57, 64, 76, 90, 126, 176
CviKI_1 RGCY 6 cut(s) 57, 64, 76, 90, 126, 176
CviQI GTAC 1 cut(s) 294
DpnI GATC 2 cut(s) 189, 327
DpnII GATC 2 cut(s) 187, 325
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 209, 239
FaiI YATR 7 cut(s) 51, 93, 184, 186, 207, 237, 258
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 1 cut(s) 286
FatI CATG 2 cut(s) 205, 235
FokI GGATG 1 cut(s) 87
FspBI CTAG 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 2 cut(s) 90, 126
Hin1II CATG 2 cut(s) 209, 239
HinfI GANTC 2 cut(s) 152, 220
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 2 cut(s) 21, 45
HpyAV CCTTC 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 2 cut(s) 113, 149
Hsp92II CATG 2 cut(s) 209, 239
Kzo9I GATC 2 cut(s) 187, 325
LmnI GCTCC 1 cut(s) 81
LpnPI CCDG 6 cut(s) 21, 30, 65, 92, 156, 276
LweI GCATC 3 cut(s) 100, 122, 136
MaeI CTAG 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 189, 327
MboI GATC 2 cut(s) 187, 325
MboII GAAGA 1 cut(s) 19
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 2 cut(s) 288, 306
MluNI TGGCCA 1 cut(s) 90
MlyI GAGTC 2 cut(s) 146, 229
Mox20I TGGCCA 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 115
MscI TGGCCA 1 cut(s) 90
MseI TTAA 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
NdeII GATC 2 cut(s) 187, 325
NlaIII CATG 2 cut(s) 209, 239
NsiI ATGCAT 1 cut(s) 115
NspI RCATGY 1 cut(s) 209
PaeI GCATGC 1 cut(s) 209
PceI AGGCCT 1 cut(s) 126
PfoI TCCNGGA 1 cut(s) 78
PleI GAGTC 2 cut(s) 146, 228
PpsI GAGTC 2 cut(s) 146, 228
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 287
Sau3AI GATC 2 cut(s) 187, 325
SchI GAGTC 2 cut(s) 146, 229
ScrFI CCNGG 1 cut(s) 80
SetI ASST 4 cut(s) 40, 78, 178, 279
SfaNI GCATC 3 cut(s) 100, 122, 136
SphI GCATGC 1 cut(s) 209
Sse9I AATT 2 cut(s) 288, 306
SseBI AGGCCT 1 cut(s) 126
SspMI CTAG 1 cut(s) 21
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 97
TasI AATT 2 cut(s) 288, 306
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 224
TspRI CASTG 1 cut(s) 112
XbaI TCTAGA 1 cut(s) 20
XceI RCATGY 1 cut(s) 209
XspI CTAG 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.