Rh7DG150300

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0025149
Physical Location & Seq
Reverse (-)
12936968 .. 12937646
679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG150300.1

Sequence Viewer

Length: 378 bp
ATGCCAATGGAGAAGAACAAAGTTTCTAGAGTTTGTGTTACAGGTGGTTCTGGATATAACGGCTGTTGGCTTGTCAAAAAGCTCCTGGAGAATGGCCATACAGTCCATCCCATTGTCAGAAACTTAGAGGATGCATCAAAAGTAGGCCTTCTGAAGTCTCTCCCTGATGCAGACTCCAAGTTATTGCTGTTCCAAGCTGATTTATATGATCCTAATGAGTTTGAGCATGCTATTCAAGGGAGTCAGTTTGTTTTTCATGTTGCCACTCCTACTGAACATAACCCTGAAATCTCCAAGTACAAGGACATAGGTGAAGCAGCTGTTGCTGGGATGAGAAGCATTGCTGATTCTTGCATTCGATCGCAGACAGTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

125

Amino Acids

13.74

Weight (kDa)

6.5

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 9 - 109 2.6e-11 NmrA-like family
Epimerase PF01370 11 - 121 3.8e-11 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 13 - 105 2.3e-09 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 13 - 121 1.2e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 15 - 100 3.6e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025149)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0195251
rosa_roxburghii Rroxscaffold_3G00260640
rosa_samantha Rh7DG150300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 203
AcoI YGGCCR 1 cut(s) 94
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 1 cut(s) 299
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 3 cut(s) 82, 197, 320
AluI AGCT 3 cut(s) 82, 197, 320
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 1 cut(s) 203
AoxI GGCC 2 cut(s) 94, 145
ApeKI GCWGC 1 cut(s) 317
AsuHPI GGTGA 1 cut(s) 323
BalI TGGCCA 1 cut(s) 96
BbvI GCAGC 1 cut(s) 329
BccI CCATC 1 cut(s) 114
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
Bme1390I CCNGG 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 3 cut(s) 121, 143, 157
BpmI CTGGAG 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 86
BseGI GGATG 3 cut(s) 106, 136, 336
BseMI GCAATG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 329
BseYI CCCAGC 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 2 cut(s) 96, 147
BsiEI CGRYCG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 2 cut(s) 96, 147
Bsp143I GATC 2 cut(s) 208, 359
BspANI GGCC 2 cut(s) 96, 147
BspPI GGATC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 339
BssMI GATC 2 cut(s) 208, 359
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 2 cut(s) 103, 370
BstAPI GCANNNNNTGC 1 cut(s) 323
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 124
BstF5I GGATG 3 cut(s) 106, 136, 336
BstKTI GATC 2 cut(s) 211, 362
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 2 cut(s) 208, 359
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 329
BsuRI GGCC 2 cut(s) 96, 147
BtsCI GGATG 3 cut(s) 106, 136, 336
Cac8I GCNNGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 298
CviAII CATG 2 cut(s) 227, 257
CviJI RGCY 7 cut(s) 63, 70, 82, 96, 147, 197, 320
CviKI_1 RGCY 7 cut(s) 63, 70, 82, 96, 147, 197, 320
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 1 cut(s) 124
DpnI GATC 2 cut(s) 210, 361
DpnII GATC 2 cut(s) 208, 359
EaeI YGGCCR 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 136
FaeI CATG 2 cut(s) 230, 260
FaiI YATR 8 cut(s) 57, 99, 205, 207, 228, 258, 279, 308
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FatI CATG 2 cut(s) 226, 256
Fnu4HI GCNGC 1 cut(s) 318
FokI GGATG 3 cut(s) 93, 143, 343
Fsp4HI GCNGC 1 cut(s) 318
FspBI CTAG 1 cut(s) 27
GluI GCNGC 1 cut(s) 318
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 2 cut(s) 96, 147
Hin1II CATG 2 cut(s) 230, 260
HinfI GANTC 3 cut(s) 173, 241, 347
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 2 cut(s) 119, 153
Hpy188III TCNNGA 2 cut(s) 27, 51
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 2 cut(s) 103, 370
HpyCH4V TGCA 3 cut(s) 134, 170, 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 1 cut(s) 124
Hsp92II CATG 2 cut(s) 230, 260
Kzo9I GATC 2 cut(s) 208, 359
LmnI GCTCC 1 cut(s) 87
LpnPI CCDG 7 cut(s) 27, 36, 71, 98, 177, 297, 312
Lsp1109I GCAGC 1 cut(s) 329
LweI GCATC 3 cut(s) 121, 143, 157
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 210, 361
MboI GATC 2 cut(s) 208, 359
MboII GAAGA 1 cut(s) 25
MlsI TGGCCA 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 96
MlyI GAGTC 2 cut(s) 167, 250
MnlI CCTC 1 cut(s) 121
Mox20I TGGCCA 1 cut(s) 96
Mph1103I ATGCAT 1 cut(s) 136
MscI TGGCCA 1 cut(s) 96
MseI TTAA 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 96
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 2 cut(s) 208, 359
NlaIII CATG 2 cut(s) 230, 260
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PceI AGGCCT 1 cut(s) 147
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 1 cut(s) 319
Ple19I CGATCG 1 cut(s) 362
PleI GAGTC 2 cut(s) 167, 249
PpsI GAGTC 2 cut(s) 167, 249
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 1 cut(s) 84
PvuI CGATCG 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 2 cut(s) 208, 359
SchI GAGTC 2 cut(s) 167, 250
ScrFI CCNGG 1 cut(s) 86
SetI ASST 5 cut(s) 46, 84, 199, 313, 322
SfaNI GCATC 3 cut(s) 121, 143, 157
SphI GCATGC 1 cut(s) 230
SseBI AGGCCT 1 cut(s) 147
SspMI CTAG 1 cut(s) 27
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 84
TaaI ACNGT 2 cut(s) 103, 370
TaqI TCGA 1 cut(s) 358
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseI GCWGC 1 cut(s) 317
TspDTI ATGAA 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 1 cut(s) 230
XspI CTAG 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.