RchiOBHm_Chr7g0233241

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0023544
Physical Location & Seq
Forward (+)
57669433 .. 57670611
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20905

Sequence Viewer

Length: 666 bp
ATGATAAGTATGGGTATGCATCCTGATGAATTCACAAACACAACCTTGATCAATGCTTACTGCAAGGAAGGGGATTTGAATAAGGCTCTTCACTTGAATGATGAAATGATAAAGAAAGGTTTCCTACCTGATGTTGTGACCTACAGTGTGCTTATCAATGGACTTAACAAACGAGCTCGGACAAGAGAAGCAAAGAAGCTTCTACTCAAGTTGTTCTATGATAACTCTGCCCCAGATGATGTCACATACAATACTCTAATAGAGAGCTGCACGAATGTTGAATTTAAGAGTGTGGTAGCCCTTGTAAAGGGATTCTGCATGAAGGGTTTGATGAAAGAAGCAGATCAAGTTTTTGAACAGGAAGGAAATGTTCAGAAGGCATTGAATCTGTACAAGGAAATGTTGCTTTCTGGTTTTCTTCCTCATACTGTGACTGTTATTGTGCTGATTAAAGAACTTTTCACTGAGGGAATGAACAATGAACTGAGTCAAGTCATAGAGAACACTCTGAGGAGCTGTGAGCTTACTGATGCTGAGCTTGCAAAGCTACTTGTTGAAACAAACCATAAAGAAGGAAATATGGATGCAGTTTTTAATGTCCTTAGTGAAATGGCCAAGGATGGTCTCCTTCCAAACAGTGGAGCAAGCGCTTGTGCAGGGGGGTAA

Protein Analysis

221

Amino Acids

24.46

Weight (kDa)

5.09

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 4 - 36 5.7e-10 PPR repeat
PPR_3 PF13812 5 - 53 9.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 8 - 56 2.2e-17 PPR repeat family
PPR PF01535 14 - 41 6.3e-06 PPR repeat
PPR_1 PF12854 39 - 68 2.5e-06 PPR repeat
PPR_2 PF13041 43 - 91 2.9e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023544)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0233241
rosa_samantha Rh7AG435500 Rh7CG454600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 638
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 2 cut(s) 29, 281
AfaI GTAC 1 cut(s) 392
AfeI AGCGCT 1 cut(s) 649
AfiI CCNNNNNNNGG 2 cut(s) 307, 638
AgsI TTSAA 6 cut(s) 79, 97, 281, 356, 385, 557
AluBI AGCT 7 cut(s) 176, 199, 267, 516, 523, 538, 547
AluI AGCT 7 cut(s) 176, 199, 267, 516, 523, 538, 547
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 629
Aor51HI AGCGCT 1 cut(s) 649
AoxI GGCC 1 cut(s) 612
ApeKI GCWGC 1 cut(s) 267
ApoI RAATTY 2 cut(s) 29, 281
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 650
BalI TGGCCA 1 cut(s) 614
BanII GRGCYC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 254
BccI CCATC 1 cut(s) 614
BclI TGATCA 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 142
BfoI RGCGCY 1 cut(s) 651
BisI GCNGC 1 cut(s) 268
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 1 cut(s) 269
BmsI GCATC 3 cut(s) 28, 520, 574
Bpu1102I GCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 1 cut(s) 615
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 638
BseDI CCNNGG 1 cut(s) 615
BseGI GGATG 3 cut(s) 19, 589, 625
BseLI CCNNNNNNNGG 2 cut(s) 307, 638
BseMII CTCAG 4 cut(s) 456, 476, 500, 525
BseRI GAGGAG 1 cut(s) 526
BseXI GCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 253
BshFI GGCC 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 307, 638
BsmAI GTCTC 1 cut(s) 629
BsnI GGCC 1 cut(s) 614
Bso31I GGTCTC 1 cut(s) 629
Bsp1286I GDGCHC 1 cut(s) 178
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 2 cut(s) 48, 343
Bsp1720I GCTNAGC 1 cut(s) 534
BspANI GGCC 1 cut(s) 614
BspCNI CTCAG 4 cut(s) 457, 477, 501, 526
BspQI GCTCTTC 1 cut(s) 93
BspTNI GGTCTC 1 cut(s) 629
BsrGI TGTACA 1 cut(s) 390
BssECI CCNNGG 1 cut(s) 615
BssMI GATC 2 cut(s) 48, 343
BssT1I CCWWGG 1 cut(s) 615
Bst4CI ACNGT 4 cut(s) 146, 430, 436, 638
Bst6I CTCTTC 1 cut(s) 93
BstAUI TGTACA 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 540, 646
BstDEI CTNAG 5 cut(s) 465, 485, 509, 534, 602
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 3 cut(s) 19, 589, 625
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 2 cut(s) 51, 346
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 2 cut(s) 48, 343
BstMWI GCNNNNNNNGC 2 cut(s) 539, 544
BstSFI CTRYAG 1 cut(s) 142
BstV1I GCAGC 1 cut(s) 254
BsuRI GGCC 1 cut(s) 614
BtsCI GGATG 3 cut(s) 19, 589, 625
BtsIMutI CAGTG 3 cut(s) 151, 462, 643
Cac8I GCNNGC 2 cut(s) 540, 646
CfoI GCGC 1 cut(s) 650
Csp6I GTAC 1 cut(s) 391
CviAII CATG 1 cut(s) 319
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 5 cut(s) 465, 485, 509, 534, 602
DpnI GATC 2 cut(s) 50, 345
DpnII GATC 2 cut(s) 48, 343
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 615
Eco24I GRGCYC 1 cut(s) 178
Eco31I GGTCTC 1 cut(s) 629
Eco47III AGCGCT 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 615
EcoT22I ATGCAT 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 615
FaeI CATG 1 cut(s) 322
FaiI YATR 9 cut(s) 11, 17, 219, 247, 320, 426, 497, 567, 581
FatI CATG 1 cut(s) 318
FbaI TGATCA 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 3 cut(s) 6, 596, 632
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 1 cut(s) 268
GlaI GCGC 1 cut(s) 649
GluI GCNGC 1 cut(s) 268
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 1 cut(s) 614
HhaI GCGC 1 cut(s) 650
Hin1II CATG 1 cut(s) 322
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 3 cut(s) 312, 385, 487
Hpy188I TCNGA 3 cut(s) 180, 375, 510
Hpy188III TCNNGA 1 cut(s) 23
HpyAV CCTTC 6 cut(s) 62, 316, 356, 370, 566, 638
HpyCH4III ACNGT 4 cut(s) 146, 430, 436, 638
HpyCH4V TGCA 7 cut(s) 19, 63, 270, 318, 542, 587, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 544
HpyF3I CTNAG 5 cut(s) 465, 485, 509, 534, 602
Hsp92II CATG 1 cut(s) 322
HspAI GCGC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 2 cut(s) 48, 343
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 513, 641
LpnPI CCDG 6 cut(s) 36, 141, 246, 344, 396, 642
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 3 cut(s) 28, 520, 574
MaeIII GTNAC 3 cut(s) 136, 241, 430
MalI GATC 2 cut(s) 50, 345
MboI GATC 2 cut(s) 48, 343
MboII GAAGA 2 cut(s) 80, 410
MhlI GDGCHC 1 cut(s) 178
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 2 cut(s) 29, 281
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 1 cut(s) 496
MnlI CCTC 3 cut(s) 432, 460, 504
Mox20I TGGCCA 1 cut(s) 614
Mph1103I ATGCAT 1 cut(s) 21
MroXI GAANNNNTTC 1 cut(s) 119
MscI TGGCCA 1 cut(s) 614
MseI TTAA 4 cut(s) 165, 285, 450, 594
MslI CAYNNNNRTG 2 cut(s) 24, 96
Msp20I TGGCCA 1 cut(s) 614
MwoI GCNNNNNNNGC 2 cut(s) 539, 544
NdeII GATC 2 cut(s) 48, 343
NlaIII CATG 1 cut(s) 322
NmuCI GTSAC 3 cut(s) 136, 241, 430
NsiI ATGCAT 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 312, 385
PflMI CCANNNNNTGG 1 cut(s) 638
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 178
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 2 cut(s) 24, 96
SacI GAGCTC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 4 cut(s) 165, 285, 450, 594
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 2 cut(s) 48, 343
SchI GAGTC 1 cut(s) 496
SduI GDGCHC 1 cut(s) 178
SfaNI GCATC 3 cut(s) 28, 520, 574
SfcI CTRYAG 1 cut(s) 142
SmiMI CAYNNNNRTG 2 cut(s) 24, 96
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 2 cut(s) 29, 281
SstI GAGCTC 1 cut(s) 178
StyI CCWWGG 1 cut(s) 615
TaaI ACNGT 4 cut(s) 146, 430, 436, 638
TasI AATT 2 cut(s) 29, 281
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 2 cut(s) 312, 385
Tru1I TTAA 4 cut(s) 165, 285, 450, 594
Tru9I TTAA 4 cut(s) 165, 285, 450, 594
TscAI CASTG 3 cut(s) 151, 469, 643
TseFI GTSAC 3 cut(s) 136, 241, 430
TseI GCWGC 1 cut(s) 267
Tsp45I GTSAC 3 cut(s) 136, 241, 430
TspDTI ATGAA 6 cut(s) 42, 117, 335, 347, 488, 495
TspRI CASTG 3 cut(s) 151, 469, 643
Van91I CCANNNNNTGG 1 cut(s) 638
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 2 cut(s) 29, 281
XmnI GAANNNNTTC 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.