RchiOBHm_Chr7g0240721

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0019442
Physical Location & Seq
Reverse (-)
66751734 .. 66752086
353 bp
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UTR
Exon/CDS
Intron
PRQ21574

Sequence Viewer

Length: 153 bp
ATGGATTCGCAGACCCAGAACACGTCGCTGCAGTGGCTTCAGAATGTGGAGAAGAGAATCGTGAAGGTTTTGGAGCTGGCCGCAAGTGTGATGGACGAGCTTGCAAACCCTACTGGTCCCAGAAGGGAGTCCATCAACAACCATGTCCGCTAG

Protein Analysis

50

Amino Acids

5.72

Weight (kDa)

6.54

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med11 PF10280 8 - 48 4.2e-06 Mediator complex protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019442)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0240721
rosa_rugosa Rorug07G0338400 Rorug07G0338400
rosa_samantha Rh6BG097900 Rh6CG093900 Rh6DG087900 Rh7AG493200
rosa_wichuraiana Rw6G009050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 81, 148
AcoI YGGCCR 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 21
AjiI CACGTC 1 cut(s) 24
AluBI AGCT 2 cut(s) 76, 100
AluI AGCT 2 cut(s) 76, 100
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 116
AvaII GGWCC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 15
BccI CCATC 2 cut(s) 85, 140
BfaI CTAG 1 cut(s) 151
BfmI CTRYAG 1 cut(s) 29
BisI GCNGC 2 cut(s) 29, 81
BlsI GCNGC 2 cut(s) 30, 82
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 15
BshFI GGCC 1 cut(s) 80
BslFI GGGAC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 1 cut(s) 80
BspACI CCGC 2 cut(s) 81, 148
BspANI GGCC 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 118
BspMAI CTGCAG 1 cut(s) 33
BsrI ACTGG 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 47
BstC8I GCNNGC 2 cut(s) 78, 102
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstSFI CTRYAG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 80
BtrI CACGTC 1 cut(s) 24
BtsI GCAGTG 1 cut(s) 38
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 78, 102
Cfr13I GGNCC 1 cut(s) 116
CviAII CATG 1 cut(s) 143
CviJI RGCY 4 cut(s) 37, 76, 80, 100
CviKI_1 RGCY 4 cut(s) 37, 76, 80, 100
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 23
FaeI CATG 1 cut(s) 146
FaiI YATR 1 cut(s) 144
FaqI GGGAC 1 cut(s) 102
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 29, 81
Fsp4HI GCNGC 2 cut(s) 29, 81
FspBI CTAG 1 cut(s) 151
GluI GCNGC 2 cut(s) 29, 81
HaeIII GGCC 1 cut(s) 80
Hin1II CATG 1 cut(s) 146
HinfI GANTC 3 cut(s) 5, 57, 128
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 58, 117
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 31, 104
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 1 cut(s) 146
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 4 cut(s) 29, 62, 99, 133
Lsp1109I GCAGC 1 cut(s) 15
MaeI CTAG 1 cut(s) 151
MaeII ACGT 1 cut(s) 23
MboII GAAGA 1 cut(s) 64
MlyI GAGTC 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 34
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 5, 57
PkrI GCNGC 2 cut(s) 30, 82
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 116
PstI CTGCAG 1 cut(s) 33
SatI GCNGC 2 cut(s) 29, 81
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 1 cut(s) 137
SetI ASST 4 cut(s) 26, 69, 78, 102
SfcI CTRYAG 1 cut(s) 29
SgeI CNNG 9 cut(s) 28, 34, 73, 89, 96, 109, 113, 126, 132
SinI GGWCC 1 cut(s) 116
SsiI CCGC 2 cut(s) 81, 148
SspMI CTAG 1 cut(s) 151
TaiI ACGT 1 cut(s) 26
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 5, 57
TscAI CASTG 1 cut(s) 38
TseI GCWGC 1 cut(s) 28
TspRI CASTG 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 116
XspI CTAG 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.