Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr7g0242261 |
| rosa_samantha | Rh7AG469800 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 114 |
| AgsI | TTSAA | 1 cut(s) | 95 |
| AluBI | AGCT | 1 cut(s) | 10 |
| AluI | AGCT | 1 cut(s) | 10 |
| BccI | CCATC | 1 cut(s) | 58 |
| BglII | AGATCT | 1 cut(s) | 19 |
| Bse1I | ACTGG | 2 cut(s) | 102, 114 |
| BseNI | ACTGG | 2 cut(s) | 102, 114 |
| Bsp143I | GATC | 1 cut(s) | 19 |
| BsrI | ACTGG | 2 cut(s) | 102, 114 |
| BssMI | GATC | 1 cut(s) | 19 |
| BstDEI | CTNAG | 1 cut(s) | 11 |
| BstKTI | GATC | 1 cut(s) | 22 |
| BstMBI | GATC | 1 cut(s) | 19 |
| BstX2I | RGATCY | 1 cut(s) | 19 |
| BstYI | RGATCY | 1 cut(s) | 19 |
| BtsIMutI | CAGTG | 2 cut(s) | 59, 107 |
| Csp6I | GTAC | 1 cut(s) | 113 |
| CviJI | RGCY | 1 cut(s) | 10 |
| CviKI_1 | RGCY | 1 cut(s) | 10 |
| CviQI | GTAC | 1 cut(s) | 113 |
| DdeI | CTNAG | 1 cut(s) | 11 |
| DpnI | GATC | 1 cut(s) | 21 |
| DpnII | GATC | 1 cut(s) | 19 |
| FaiI | YATR | 1 cut(s) | 86 |
| FspEI | CC | 5 cut(s) | 27, 50, 66, 83, 95 |
| HinfI | GANTC | 1 cut(s) | 56 |
| Hpy188III | TCNNGA | 1 cut(s) | 53 |
| HpyF3I | CTNAG | 1 cut(s) | 11 |
| Kzo9I | GATC | 1 cut(s) | 19 |
| LpnPI | CCDG | 3 cut(s) | 66, 83, 95 |
| MalI | GATC | 1 cut(s) | 21 |
| MboI | GATC | 1 cut(s) | 19 |
| MboII | GAAGA | 1 cut(s) | 65 |
| MflI | RGATCY | 1 cut(s) | 19 |
| NdeII | GATC | 1 cut(s) | 19 |
| PfeI | GAWTC | 1 cut(s) | 56 |
| PsuI | RGATCY | 1 cut(s) | 19 |
| RsaI | GTAC | 1 cut(s) | 114 |
| RsaNI | GTAC | 1 cut(s) | 113 |
| Sau3AI | GATC | 1 cut(s) | 19 |
| SetI | ASST | 1 cut(s) | 12 |
| SgeI | CNNG | 3 cut(s) | 65, 110, 122 |
| TfiI | GAWTC | 1 cut(s) | 56 |
| TscAI | CASTG | 2 cut(s) | 66, 114 |
| TspRI | CASTG | 2 cut(s) | 66, 114 |