Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr7g0242261 |
| rosa_samantha | Rh7AG469800 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 223 |
| AciI | CCGC | 1 cut(s) | 86 |
| AgsI | TTSAA | 1 cut(s) | 328 |
| AjnI | CCWGG | 1 cut(s) | 128 |
| AluBI | AGCT | 2 cut(s) | 10, 182 |
| AluI | AGCT | 2 cut(s) | 10, 182 |
| ApeKI | GCWGC | 1 cut(s) | 273 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 318 |
| AsuII | TTCGAA | 1 cut(s) | 304 |
| BaeGI | GKGCMC | 1 cut(s) | 226 |
| BanI | GGYRCC | 1 cut(s) | 223 |
| BbsI | GAAGAC | 1 cut(s) | 289 |
| BbvI | GCAGC | 1 cut(s) | 285 |
| BccI | CCATC | 1 cut(s) | 115 |
| BciT130I | CCWGG | 1 cut(s) | 130 |
| BfaI | CTAG | 3 cut(s) | 201, 270, 278 |
| BglII | AGATCT | 1 cut(s) | 19 |
| BisI | GCNGC | 1 cut(s) | 274 |
| BlsI | GCNGC | 1 cut(s) | 275 |
| Bme1390I | CCNGG | 1 cut(s) | 130 |
| BmiI | GGNNCC | 1 cut(s) | 225 |
| BmrFI | CCNGG | 1 cut(s) | 130 |
| BpiI | GAAGAC | 1 cut(s) | 289 |
| Bpu14I | TTCGAA | 1 cut(s) | 304 |
| BsaAI | YACGTR | 1 cut(s) | 141 |
| BsaJI | CCNNGG | 1 cut(s) | 129 |
| Bse1I | ACTGG | 1 cut(s) | 291 |
| BseBI | CCWGG | 1 cut(s) | 130 |
| BseDI | CCNNGG | 1 cut(s) | 129 |
| BseGI | GGATG | 1 cut(s) | 126 |
| BseNI | ACTGG | 1 cut(s) | 291 |
| BseSI | GKGCMC | 1 cut(s) | 226 |
| BseXI | GCAGC | 1 cut(s) | 285 |
| BshNI | GGYRCC | 1 cut(s) | 223 |
| Bsp119I | TTCGAA | 1 cut(s) | 304 |
| Bsp1286I | GDGCHC | 1 cut(s) | 226 |
| Bsp143I | GATC | 1 cut(s) | 19 |
| BspACI | CCGC | 1 cut(s) | 86 |
| BspLI | GGNNCC | 1 cut(s) | 225 |
| BspT104I | TTCGAA | 1 cut(s) | 304 |
| BspT107I | GGYRCC | 1 cut(s) | 223 |
| BsrI | ACTGG | 1 cut(s) | 291 |
| BssECI | CCNNGG | 1 cut(s) | 129 |
| BssMI | GATC | 1 cut(s) | 19 |
| Bst2UI | CCWGG | 1 cut(s) | 130 |
| Bst4CI | ACNGT | 3 cut(s) | 166, 197, 209 |
| BstBAI | YACGTR | 1 cut(s) | 141 |
| BstBI | TTCGAA | 1 cut(s) | 304 |
| BstDEI | CTNAG | 1 cut(s) | 11 |
| BstF5I | GGATG | 1 cut(s) | 126 |
| BstKTI | GATC | 1 cut(s) | 22 |
| BstMBI | GATC | 1 cut(s) | 19 |
| BstNI | CCWGG | 1 cut(s) | 130 |
| BstSCI | CCNGG | 1 cut(s) | 128 |
| BstSLI | GKGCMC | 1 cut(s) | 226 |
| BstSNI | TACGTA | 1 cut(s) | 141 |
| BstV1I | GCAGC | 1 cut(s) | 285 |
| BstV2I | GAAGAC | 1 cut(s) | 289 |
| BstX2I | RGATCY | 1 cut(s) | 19 |
| BstYI | RGATCY | 1 cut(s) | 19 |
| BtsCI | GGATG | 1 cut(s) | 126 |
| BtsIMutI | CAGTG | 1 cut(s) | 298 |
| CviAII | CATG | 1 cut(s) | 229 |
| CviJI | RGCY | 5 cut(s) | 10, 182, 252, 276, 281 |
| CviKI_1 | RGCY | 5 cut(s) | 10, 182, 252, 276, 281 |
| DdeI | CTNAG | 1 cut(s) | 11 |
| DpnI | GATC | 1 cut(s) | 21 |
| DpnII | GATC | 1 cut(s) | 19 |
| EciI | GGCGGA | 1 cut(s) | 101 |
| Eco105I | TACGTA | 1 cut(s) | 141 |
| EcoRII | CCWGG | 1 cut(s) | 128 |
| FaeI | CATG | 1 cut(s) | 232 |
| FaiI | YATR | 3 cut(s) | 64, 186, 230 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 303, 335 |
| FatI | CATG | 1 cut(s) | 228 |
| Fnu4HI | GCNGC | 1 cut(s) | 274 |
| FokI | GGATG | 1 cut(s) | 133 |
| Fsp4HI | GCNGC | 1 cut(s) | 274 |
| FspBI | CTAG | 3 cut(s) | 201, 270, 278 |
| GluI | GCNGC | 1 cut(s) | 274 |
| Hin1II | CATG | 1 cut(s) | 232 |
| HinfI | GANTC | 1 cut(s) | 125 |
| Hpy166II | GTNNAC | 1 cut(s) | 193 |
| Hpy188I | TCNGA | 1 cut(s) | 179 |
| Hpy188III | TCNNGA | 1 cut(s) | 53 |
| Hpy8I | GTNNAC | 1 cut(s) | 193 |
| HpyAV | CCTTC | 1 cut(s) | 300 |
| HpyCH4III | ACNGT | 3 cut(s) | 166, 197, 209 |
| HpyCH4IV | ACGT | 1 cut(s) | 140 |
| HpyCH4V | TGCA | 2 cut(s) | 66, 76 |
| HpyF3I | CTNAG | 1 cut(s) | 11 |
| HpySE526I | ACGT | 1 cut(s) | 140 |
| Hsp92II | CATG | 1 cut(s) | 232 |
| Kzo9I | GATC | 1 cut(s) | 19 |
| LpnPI | CCDG | 5 cut(s) | 66, 115, 142, 244, 304 |
| Lsp1109I | GCAGC | 1 cut(s) | 285 |
| MaeI | CTAG | 3 cut(s) | 201, 270, 278 |
| MaeII | ACGT | 1 cut(s) | 140 |
| MaeIII | GTNAC | 1 cut(s) | 203 |
| MalI | GATC | 1 cut(s) | 21 |
| MboI | GATC | 1 cut(s) | 19 |
| MboII | GAAGA | 1 cut(s) | 289 |
| MflI | RGATCY | 1 cut(s) | 19 |
| MhlI | GDGCHC | 1 cut(s) | 226 |
| MmeI | TCCRAC | 1 cut(s) | 311 |
| MnlI | CCTC | 2 cut(s) | 231, 294 |
| MroXI | GAANNNNTTC | 1 cut(s) | 318 |
| MspR9I | CCNGG | 1 cut(s) | 130 |
| MvaI | CCWGG | 1 cut(s) | 130 |
| NdeII | GATC | 1 cut(s) | 19 |
| NlaIII | CATG | 1 cut(s) | 232 |
| NlaIV | GGNNCC | 1 cut(s) | 225 |
| NspV | TTCGAA | 1 cut(s) | 304 |
| PdmI | GAANNNNTTC | 1 cut(s) | 318 |
| PfeI | GAWTC | 1 cut(s) | 125 |
| PkrI | GCNGC | 1 cut(s) | 275 |
| Ppu21I | YACGTR | 1 cut(s) | 141 |
| Psp6I | CCWGG | 1 cut(s) | 128 |
| PspGI | CCWGG | 1 cut(s) | 128 |
| PspN4I | GGNNCC | 1 cut(s) | 225 |
| PsuI | RGATCY | 1 cut(s) | 19 |
| SatI | GCNGC | 1 cut(s) | 274 |
| Sau3AI | GATC | 1 cut(s) | 19 |
| ScrFI | CCNGG | 1 cut(s) | 130 |
| SduI | GDGCHC | 1 cut(s) | 226 |
| SetI | ASST | 3 cut(s) | 12, 143, 184 |
| SfuI | TTCGAA | 1 cut(s) | 304 |
| SgeI | CNNG | 9 cut(s) | 65, 141, 142, 213, 241, 271, 282, 290, 303 |
| SnaBI | TACGTA | 1 cut(s) | 141 |
| SsiI | CCGC | 1 cut(s) | 86 |
| SspMI | CTAG | 3 cut(s) | 201, 270, 278 |
| StyD4I | CCNGG | 1 cut(s) | 128 |
| TaaI | ACNGT | 3 cut(s) | 166, 197, 209 |
| TaiI | ACGT | 1 cut(s) | 143 |
| TaqI | TCGA | 1 cut(s) | 304 |
| TfiI | GAWTC | 1 cut(s) | 125 |
| TscAI | CASTG | 1 cut(s) | 298 |
| TseI | GCWGC | 1 cut(s) | 273 |
| TspRI | CASTG | 1 cut(s) | 298 |
| XmnI | GAANNNNTTC | 1 cut(s) | 318 |
| XspI | CTAG | 3 cut(s) | 201, 270, 278 |