RCHIOBHM_MTG0498341

NADH-ubiquinone/plastoquinone oxidoreductase chain 4L

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0027568
Physical Location & Seq
Forward (+)
60618 .. 60912
295 bp
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UTR
Exon/CDS
Intron
PRQ15606

Sequence Viewer

Length: 273 bp
ATGATCATCTCTATTTTAGGTATTCGGGGAATCCTCCTTAATAGACGAAATATTCCTATTATGTCAATGCCAATTGAATCAATGTTATTAGCCGTGAATTCGAACTTTTTGGTATTTTCCGTTTCTTCGGATGATATGATGGGTCAATCATTTGCTTCATTGGTTCCAACGGTGGCAGCTGCGGAATCTGCTATTGGGTTAGCCATTTTCGTTATTACTTTCCGAGTCCGAGGGACTATTGCTGTGGAATCTATTAATAGCATTCAAGGTTAA

Protein Analysis

90

Amino Acids

9.6

Weight (kDa)

6.04

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q2 PF00420 1 - 89 8.3e-18 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0027568)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00650
rosa_chinensis RchiOBHm_MTg0498341

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 182
AcsI RAATTY 1 cut(s) 97
AgsI TTSAA 2 cut(s) 77, 266
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
ApeKI GCWGC 2 cut(s) 176, 179
ApoI RAATTY 1 cut(s) 97
AseI ATTAAT 1 cut(s) 255
AsuII TTCGAA 1 cut(s) 101
BbvI GCAGC 2 cut(s) 166, 188
BccI CCATC 1 cut(s) 133
BceAI ACGGC 1 cut(s) 77
BclI TGATCA 1 cut(s) 3
BisI GCNGC 2 cut(s) 177, 180
BlsI GCNGC 2 cut(s) 178, 181
BmiI GGNNCC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 166, 188
BslFI GGGAC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 101
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 1 cut(s) 3
Bst4CI ACNGT 1 cut(s) 172
BstBI TTCGAA 1 cut(s) 101
BstF5I GGATG 1 cut(s) 136
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 166, 188
BtsCI GGATG 1 cut(s) 136
CviJI RGCY 3 cut(s) 92, 179, 203
CviKI_1 RGCY 3 cut(s) 92, 179, 203
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EcoRI GAATTC 1 cut(s) 97
FaiI YATR 2 cut(s) 62, 137
FaqI GGGAC 1 cut(s) 247
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 177, 180
FokI GGATG 1 cut(s) 143
Fsp4HI GCNGC 2 cut(s) 177, 180
GluI GCNGC 2 cut(s) 177, 180
HinfI GANTC 5 cut(s) 30, 77, 185, 225, 248
Hpy188I TCNGA 3 cut(s) 130, 224, 230
HpyCH4III ACNGT 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
Lsp1109I GCAGC 2 cut(s) 166, 188
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 1 cut(s) 117
MfeI CAATTG 1 cut(s) 72
MluCI AATT 2 cut(s) 72, 97
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 2 cut(s) 44, 224
MseI TTAA 3 cut(s) 39, 255, 271
MspA1I CMGCKG 1 cut(s) 179
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 261
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 1 cut(s) 3
NlaIV GGNNCC 1 cut(s) 165
NspV TTCGAA 1 cut(s) 101
PctI GAATGC 1 cut(s) 261
PfeI GAWTC 4 cut(s) 30, 77, 185, 248
PkrI GCNGC 2 cut(s) 178, 181
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PshBI ATTAAT 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 179
SaqAI TTAA 3 cut(s) 39, 255, 271
SatI GCNGC 2 cut(s) 177, 180
Sau3AI GATC 1 cut(s) 3
SchI GAGTC 1 cut(s) 234
SetI ASST 3 cut(s) 22, 181, 271
SfuI TTCGAA 1 cut(s) 101
SgeI CNNG 4 cut(s) 38, 106, 236, 242
Sse9I AATT 2 cut(s) 72, 97
SsiI CCGC 1 cut(s) 182
SspI AATATT 1 cut(s) 52
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 1 cut(s) 101
TasI AATT 2 cut(s) 72, 97
TfiI GAWTC 4 cut(s) 30, 77, 185, 248
Tru1I TTAA 3 cut(s) 39, 255, 271
Tru9I TTAA 3 cut(s) 39, 255, 271
TseI GCWGC 2 cut(s) 176, 179
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 109
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.