RCHIOBHM_MTG0498381

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0023963
Physical Location & Seq
Forward (+)
69364 .. 69946
583 bp
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UTR
Exon/CDS
Intron
PRQ15610

Sequence Viewer

Length: 357 bp
ATGTCAGAATTTGCACCTATTTGTATCTATTTAGTGATCAGTCCGCTAGTTTCTTTGATCCCACTCGGTGTTCCTTTTCCATTTGCTTCCAATAGTTCAACCTATCCAGAAAAATTGTCGGCCTATGAATGTGGTTTCGATCCTTTCGGTGATGCCAGAAGTCGTTTCGATATACGATTTTATCTTGTTTCAATTTTATTTATTATCCTTGATCCGGAAGTAACCTTTTCCTTTCCTTGGGCAGTACCTCTCAACAAGATTGATCCGTTTGGATCTTGGTCCATGATGGCCTTTTTATTGATTTTGACGATTGGATCTCTCTATGAATGGAAAAGGGGTGCTTCGGATCGGGAGTAA

Protein Analysis

118

Amino Acids

13.36

Weight (kDa)

4.66

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q4 PF00507 30 - 114 3.7e-30 NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07751
rosa_chinensis RchiOBHm_MTg0498381
rosa_roxburghii Rroxscaffold_2G00119230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 1 cut(s) 44
AclWI GGATC 7 cut(s) 52, 134, 206, 257, 280, 322, 354
AcsI RAATTY 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 68
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 214, 237
AgsI TTSAA 2 cut(s) 99, 192
AlwI GGATC 7 cut(s) 52, 134, 206, 257, 280, 322, 354
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 2 cut(s) 120, 288
ApoI RAATTY 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 279
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 279
BccI CCATC 1 cut(s) 280
BclI TGATCA 1 cut(s) 36
BfaI CTAG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 279
BmsI GCATC 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 237
BseAI TCCGGA 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 236
BseLI CCNNNNNNNGG 2 cut(s) 214, 237
BshFI GGCC 2 cut(s) 122, 290
BsiSI CCGG 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 214, 237
BsnI GGCC 2 cut(s) 122, 290
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
BspACI CCGC 1 cut(s) 44
BspANI GGCC 2 cut(s) 122, 290
BspEI TCCGGA 1 cut(s) 214
BspPI GGATC 7 cut(s) 52, 134, 206, 257, 280, 322, 354
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
BssT1I CCWWGG 1 cut(s) 236
BstKTI GATC 8 cut(s) 39, 60, 142, 214, 265, 275, 317, 349
BstMBI GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
BstX2I RGATCY 2 cut(s) 272, 314
BstYI RGATCY 2 cut(s) 272, 314
BsuRI GGCC 2 cut(s) 122, 290
Cfr13I GGNCC 1 cut(s) 279
Csp6I GTAC 1 cut(s) 245
CviAII CATG 1 cut(s) 283
CviJI RGCY 2 cut(s) 122, 290
CviKI_1 RGCY 2 cut(s) 122, 290
CviQI GTAC 1 cut(s) 245
DpnI GATC 8 cut(s) 38, 59, 141, 213, 264, 274, 316, 348
DpnII GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
DraIII CACNNNGTG 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 1 cut(s) 286
FaiI YATR 4 cut(s) 126, 173, 284, 324
FalI AAGNNNNNCTT 2 cut(s) 325, 357
FatI CATG 1 cut(s) 282
FbaI TGATCA 1 cut(s) 36
FspBI CTAG 1 cut(s) 47
HaeIII GGCC 2 cut(s) 122, 290
HapII CCGG 1 cut(s) 215
Hin1II CATG 1 cut(s) 286
HpaII CCGG 1 cut(s) 215
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 2 cut(s) 7, 346
Hpy188III TCNNGA 3 cut(s) 107, 215, 350
HpyCH4V TGCA 1 cut(s) 14
Hsp92II CATG 1 cut(s) 286
Kpn2I TCCGGA 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
LpnPI CCDG 3 cut(s) 120, 169, 228
LweI GCATC 1 cut(s) 142
MaeI CTAG 1 cut(s) 47
MaeIII GTNAC 1 cut(s) 220
MalI GATC 8 cut(s) 38, 59, 141, 213, 264, 274, 316, 348
MboI GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
MflI RGATCY 2 cut(s) 272, 314
MluCI AATT 3 cut(s) 8, 113, 192
MnlI CCTC 1 cut(s) 258
MroI TCCGGA 1 cut(s) 214
MspI CCGG 1 cut(s) 215
NdeII GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
NlaIII CATG 1 cut(s) 286
PspPI GGNCC 1 cut(s) 279
PsuI RGATCY 2 cut(s) 272, 314
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
Sau3AI GATC 8 cut(s) 36, 57, 139, 211, 262, 272, 314, 346
Sau96I GGNCC 1 cut(s) 279
SetI ASST 4 cut(s) 19, 104, 227, 250
SfaNI GCATC 1 cut(s) 142
SinI GGWCC 1 cut(s) 279
Sse9I AATT 3 cut(s) 8, 113, 192
SsiI CCGC 1 cut(s) 44
SspMI CTAG 1 cut(s) 47
StyI CCWWGG 1 cut(s) 236
TaqI TCGA 2 cut(s) 138, 168
TasI AATT 3 cut(s) 8, 113, 192
TspDTI ATGAA 2 cut(s) 141, 339
TspGWI ACGGA 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 279
XapI RAATTY 1 cut(s) 8
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.