RLG00000000099

Cell wall-associated hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0030995
Physical Location & Seq
Reverse (-)
502517 .. 503100
584 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000099

Sequence Viewer

Length: 534 bp
ATGAGTCGCCGGCTCATTCTTCAACAGGCACGCGGTCAGAGCCCAATGCTCCTCCCACTGCTTGGGAGCTTACGGTTTCATGTTCTATTTCACTCCCCGATGGGGGTTCTTTTCACCCTTCCCTCACGGTACTACTTCACTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGTCCCATGCTACTCGGGTCAGAGCCACTCCACCGCTTCGCCTAGCAGCACGACGCTTGTATTGCTCTCCCACAACCCCGCTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCCCATGGATTAAGCAGCAGTTCGAAAGGTTAACCTATTCGGGAATCTCCGGATCTACGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTTACTACGCCCTTCCTCGTCTCTGGGTGCCTAGGTATCCACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.41

Weight (kDa)

11.78

Isoelectric Point (pI)

68.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030995)

Species Orthologous Gene IDs
rosa_laevigata RLG00000000099
rosa_multiflora Rmu_sc0000498.1_g000057

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 514
AccB7I CCANNNNNTGG 1 cut(s) 62
AccII CGCG 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 446
AciI CCGC 4 cut(s) 33, 239, 284, 320
AclWI GGATC 1 cut(s) 457
AcvI CACGTG 1 cut(s) 205
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 4 cut(s) 62, 102, 103, 402
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 23, 466
AjnI CCWGG 2 cut(s) 151, 380
AluBI AGCT 1 cut(s) 69
AluI AGCT 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 393, 512
AlwI GGATC 1 cut(s) 457
Ama87I CYCGRG 1 cut(s) 219
Aor13HI TCCGGA 1 cut(s) 446
ApeKI GCWGC 3 cut(s) 251, 301, 412
Asp700I GAANNNNTTC 1 cut(s) 479
AspA2I CCTAGG 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 177
AsuHPI GGTGA 2 cut(s) 106, 140
AsuII TTCGAA 1 cut(s) 420
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 177
AvrII CCTAGG 1 cut(s) 518
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 44
BbrPI CACGTG 1 cut(s) 205
BbvI GCAGC 3 cut(s) 263, 288, 424
BccI CCATC 1 cut(s) 94
BciT130I CCWGG 2 cut(s) 153, 382
BciVI GTATCC 1 cut(s) 534
BcoDI GTCTC 2 cut(s) 393, 512
BfaI CTAG 2 cut(s) 248, 519
BfuI GTATCC 1 cut(s) 534
BisI GCNGC 4 cut(s) 252, 302, 320, 413
BlnI CCTAGG 1 cut(s) 518
BlsI GCNGC 4 cut(s) 253, 303, 321, 414
Bme1390I CCNGG 2 cut(s) 153, 382
Bme18I GGWCC 1 cut(s) 177
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 177
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 2 cut(s) 153, 382
Bpu14I TTCGAA 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 205
BsaJI CCNNGG 3 cut(s) 151, 401, 518
BsaWI WCCGGW 1 cut(s) 446
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 102, 103, 402
Bse118I RCCGGY 1 cut(s) 9
BseAI TCCGGA 1 cut(s) 446
BseBI CCWGG 2 cut(s) 153, 382
BseDI CCNNGG 3 cut(s) 151, 401, 518
BseLI CCNNNNNNNGG 4 cut(s) 62, 102, 103, 402
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 3 cut(s) 263, 288, 424
Bsh1236I CGCG 1 cut(s) 33
BshNI GGYRCC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 2 cut(s) 10, 447
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 4 cut(s) 62, 102, 103, 402
BsmAI GTCTC 2 cut(s) 393, 512
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 219
Bsp119I TTCGAA 1 cut(s) 420
Bsp1286I GDGCHC 1 cut(s) 44
Bsp13I TCCGGA 1 cut(s) 446
Bsp143I GATC 1 cut(s) 449
Bsp19I CCATGG 1 cut(s) 401
BspACI CCGC 4 cut(s) 33, 239, 284, 320
BspEI TCCGGA 1 cut(s) 446
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 1 cut(s) 457
BspT104I TTCGAA 1 cut(s) 420
BspT107I GGYRCC 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 3 cut(s) 151, 401, 518
BssMI GATC 1 cut(s) 449
BssT1I CCWWGG 2 cut(s) 401, 518
Bst2UI CCWGG 2 cut(s) 153, 382
Bst4CI ACNGT 4 cut(s) 75, 129, 294, 531
BstBAI YACGTR 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 420
BstC8I GCNNGC 4 cut(s) 11, 31, 317, 397
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 401
BstEII GGTNACC 1 cut(s) 146
BstFNI CGCG 1 cut(s) 33
BstKTI GATC 1 cut(s) 452
BstMAI GTCTC 2 cut(s) 393, 512
BstMBI GATC 1 cut(s) 449
BstMWI GCNNNNNNNGC 3 cut(s) 39, 267, 494
BstNI CCWGG 2 cut(s) 153, 382
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 151, 380
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 263, 288, 424
BstX2I RGATCY 1 cut(s) 449
BstYI RGATCY 1 cut(s) 449
BsuI GTATCC 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 401
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 4 cut(s) 11, 31, 317, 397
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 177
CseI GACGC 1 cut(s) 267
Csp6I GTAC 1 cut(s) 130
CviAII CATG 3 cut(s) 80, 212, 402
CviJI RGCY 7 cut(s) 13, 42, 69, 165, 230, 301, 359
CviKI_1 RGCY 7 cut(s) 13, 42, 69, 165, 230, 301, 359
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 1 cut(s) 363
DpnI GATC 1 cut(s) 451
DpnII GATC 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 401, 518
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 177
Eco72I CACGTG 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 151, 380
EcoT14I CCWWGG 2 cut(s) 401, 518
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 2 cut(s) 401, 518
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 3 cut(s) 83, 215, 405
FaiI YATR 3 cut(s) 81, 213, 403
FaqI GGGAC 1 cut(s) 193
FatI CATG 3 cut(s) 79, 211, 401
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 252, 302, 320, 413
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 252, 302, 320, 413
FspBI CTAG 2 cut(s) 248, 519
GluI GCNGC 4 cut(s) 252, 302, 320, 413
HapII CCGG 2 cut(s) 10, 447
HgaI GACGC 1 cut(s) 267
Hin1II CATG 3 cut(s) 83, 215, 405
HincII GTYRAC 1 cut(s) 429
HindII GTYRAC 1 cut(s) 429
HinfI GANTC 5 cut(s) 4, 187, 198, 332, 441
HpaI GTTAAC 1 cut(s) 429
HpaII CCGG 2 cut(s) 10, 447
HphI GGTGA 2 cut(s) 106, 140
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 2 cut(s) 39, 227
Hpy188III TCNNGA 2 cut(s) 438, 447
Hpy8I GTNNAC 1 cut(s) 429
Hpy99I CGWCG 2 cut(s) 261, 488
HpyAV CCTTC 2 cut(s) 128, 509
HpyCH4III ACNGT 4 cut(s) 75, 129, 294, 531
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 267, 494
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 3 cut(s) 83, 215, 405
Kpn2I TCCGGA 1 cut(s) 446
KroI GCCGGC 1 cut(s) 9
KroNI GCCGGC 1 cut(s) 11
KspAI GTTAAC 1 cut(s) 429
Kzo9I GATC 1 cut(s) 449
LmnI GCTCC 3 cut(s) 54, 66, 309
Lsp1109I GCAGC 3 cut(s) 263, 288, 424
MaeI CTAG 2 cut(s) 248, 519
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 146
MalI GATC 1 cut(s) 451
MboI GATC 1 cut(s) 449
MboII GAAGA 1 cut(s) 11
MflI RGATCY 1 cut(s) 449
MhlI GDGCHC 1 cut(s) 44
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 4 cut(s) 62, 133, 363, 513
MroI TCCGGA 1 cut(s) 446
MroNI GCCGGC 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 2 cut(s) 408, 428
MspI CCGG 2 cut(s) 10, 447
MspR9I CCNGG 2 cut(s) 153, 382
MvaI CCWGG 2 cut(s) 153, 382
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 39, 267, 494
NaeI GCCGGC 1 cut(s) 11
NcoI CCATGG 1 cut(s) 401
NdeII GATC 1 cut(s) 449
NgoMIV GCCGGC 1 cut(s) 9
NlaIII CATG 3 cut(s) 83, 215, 405
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 146
NspV TTCGAA 1 cut(s) 420
PdiI GCCGGC 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 4 cut(s) 187, 198, 332, 441
PflMI CCANNNNNTGG 1 cut(s) 62
PkrI GCNGC 4 cut(s) 253, 303, 321, 414
PleI GAGTC 1 cut(s) 12
PmaCI CACGTG 1 cut(s) 205
PmlI CACGTG 1 cut(s) 205
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 151, 380
PspCI CACGTG 1 cut(s) 205
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 2 cut(s) 151, 380
PspN4I GGNNCC 1 cut(s) 516
PspPI GGNCC 1 cut(s) 177
PsuI RGATCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 408, 428
SatI GCNGC 4 cut(s) 252, 302, 320, 413
Sau3AI GATC 1 cut(s) 449
Sau96I GGNCC 1 cut(s) 177
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 153, 382
SduI GDGCHC 1 cut(s) 44
SetI ASST 7 cut(s) 71, 176, 207, 386, 428, 434, 524
SfuI TTCGAA 1 cut(s) 420
SinI GGWCC 1 cut(s) 177
SsiI CCGC 4 cut(s) 33, 239, 284, 320
SspMI CTAG 2 cut(s) 248, 519
StyD4I CCNGG 2 cut(s) 151, 380
StyI CCWWGG 2 cut(s) 401, 518
TaaI ACNGT 4 cut(s) 75, 129, 294, 531
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 420
TaqII GACCGA 1 cut(s) 134
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 4 cut(s) 187, 198, 332, 441
Tru1I TTAA 2 cut(s) 408, 428
Tru9I TTAA 2 cut(s) 408, 428
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 3 cut(s) 251, 301, 412
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 68
TspRI CASTG 1 cut(s) 63
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 177
XmaJI CCTAGG 1 cut(s) 518
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 2 cut(s) 248, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.