RLG00000002930

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0030539
Physical Location & Seq
Reverse (-)
41872326 .. 41873004
679 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002930

Sequence Viewer

Length: 420 bp
ATGTCATACTCTCATTTCTTCAGATGCTTCAACTCTGAGGCCTTTGTAGGTGAGATCGCCGAAGATTGGAGACGGATTATCGAGGCTAACTTTAGAGTCATGACCCGGAAGTCGAAGCAAACCAGATCCAAAAATCCTTCTATTGTGCTCGCCTTGGAGCACATTCTCTTCGCATCTTCACTGCTTGTTCACACTTCACTTGGGCGGCAATCTTTGCCGATGCCTAGAAAACCGGCCCCAAAAAAGGGATCCGCCCCAATTTGGTCTGAAAAATGGAAGCATATTAATCAAGTCGGGCCCGAAAATTTCCTCGGTGTGAATTCGCACCGGGCCCGGCCCGGTGACCTACCAATGTTCGCCTGGAGCTCGCTAATCCTTCCAAATGTGCATGTGTTCGACGGTGTGGCATTTAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

140

Amino Acids

15.8

Weight (kDa)

10.27

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0030539)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0211561
rosa_laevigata RLG00000002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 109
AciI CCGC 2 cut(s) 205, 252
AclWI GGATC 3 cut(s) 120, 243, 256
AcsI RAATTY 2 cut(s) 304, 319
AcuI CTGAAG 1 cut(s) 4
AfiI CCNNNNNNNGG 4 cut(s) 66, 244, 245, 261
AgsI TTSAA 1 cut(s) 31
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw21I GWGCWC 3 cut(s) 150, 162, 368
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 3 cut(s) 120, 243, 256
AoxI GGCC 5 cut(s) 39, 234, 296, 330, 335
ApaI GGGCCC 2 cut(s) 300, 334
ApoI RAATTY 2 cut(s) 304, 319
AseI ATTAAT 1 cut(s) 285
AspS9I GGNCC 6 cut(s) 235, 296, 297, 330, 331, 336
AsuC2I CCSGG 4 cut(s) 106, 329, 334, 339
AsuHPI GGTGA 2 cut(s) 62, 353
BaeGI GKGCMC 2 cut(s) 300, 334
BamHI GGATCC 1 cut(s) 248
BanII GRGCYC 3 cut(s) 300, 334, 368
Bbv12I GWGCWC 3 cut(s) 150, 162, 368
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 4 cut(s) 106, 329, 334, 339
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 225
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
Bme1390I CCNGG 5 cut(s) 106, 329, 334, 339, 361
BmgT120I GGNCC 6 cut(s) 235, 296, 297, 330, 331, 336
BmiI GGNNCC 4 cut(s) 237, 250, 298, 332
BmrFI CCNGG 5 cut(s) 106, 329, 334, 339, 361
BmsI GCATC 3 cut(s) 14, 182, 210
BpmI CTGGAG 1 cut(s) 382
BpuMI CCSGG 4 cut(s) 106, 329, 334, 339
BsaJI CCNNGG 2 cut(s) 153, 310
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 244, 245, 261
Bse118I RCCGGY 1 cut(s) 232
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 153, 310
BseLI CCNNNNNNNGG 4 cut(s) 66, 244, 245, 261
BseMII CTCAG 1 cut(s) 27
BseSI GKGCMC 2 cut(s) 300, 334
BshFI GGCC 5 cut(s) 41, 236, 298, 332, 337
BsiHKAI GWGCWC 3 cut(s) 150, 162, 368
BsiSI CCGG 5 cut(s) 106, 233, 328, 334, 339
BslI CCNNNNNNNGG 4 cut(s) 66, 244, 245, 261
BsmAI GTCTC 1 cut(s) 64
BsmBI CGTCTC 1 cut(s) 64
BsnI GGCC 5 cut(s) 41, 236, 298, 332, 337
Bsp120I GGGCCC 2 cut(s) 296, 330
Bsp1286I GDGCHC 5 cut(s) 150, 162, 300, 334, 368
Bsp143I GATC 3 cut(s) 54, 125, 248
BspACI CCGC 2 cut(s) 205, 252
BspANI GGCC 5 cut(s) 41, 236, 298, 332, 337
BspCNI CTCAG 1 cut(s) 28
BspHI TCATGA 1 cut(s) 99
BspLI GGNNCC 4 cut(s) 237, 250, 298, 332
BspPI GGATC 3 cut(s) 120, 243, 256
BsrFI RCCGGY 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 2 cut(s) 153, 310
BssMI GATC 3 cut(s) 54, 125, 248
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 1 cut(s) 401
Bst6I CTCTTC 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 214
BstC8I GCNNGC 2 cut(s) 150, 368
BstDEI CTNAG 1 cut(s) 36
BstEII GGTNACC 1 cut(s) 341
BstKTI GATC 3 cut(s) 57, 128, 251
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 3 cut(s) 54, 125, 248
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNI CCWGG 1 cut(s) 361
BstNSI RCATGY 1 cut(s) 392
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 5 cut(s) 104, 327, 332, 337, 359
BstSLI GKGCMC 2 cut(s) 300, 334
BstX2I RGATCY 2 cut(s) 125, 248
BstYI RGATCY 2 cut(s) 125, 248
BsuRI GGCC 5 cut(s) 41, 236, 298, 332, 337
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 150, 368
CciI TCATGA 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 6 cut(s) 235, 296, 297, 330, 331, 336
CviAII CATG 2 cut(s) 100, 389
CviJI RGCY 7 cut(s) 41, 86, 236, 298, 332, 337, 366
CviKI_1 RGCY 7 cut(s) 41, 86, 236, 298, 332, 337, 366
DdeI CTNAG 1 cut(s) 36
DpnI GATC 3 cut(s) 56, 127, 250
DpnII GATC 3 cut(s) 54, 125, 248
DrdI GACNNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 3 cut(s) 300, 334, 368
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 4
Eco91I GGTNACC 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 366
EcoO65I GGTNACC 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 359
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 3 cut(s) 300, 334, 368
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 2 cut(s) 103, 392
FaiI YATR 4 cut(s) 7, 101, 282, 390
FatI CATG 2 cut(s) 99, 388
Fnu4HI GCNGC 1 cut(s) 206
FriOI GRGCYC 3 cut(s) 300, 334, 368
Fsp4HI GCNGC 1 cut(s) 206
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 382
HaeIII GGCC 5 cut(s) 41, 236, 298, 332, 337
HapII CCGG 5 cut(s) 106, 233, 328, 334, 339
Hin1II CATG 2 cut(s) 103, 392
HinfI GANTC 1 cut(s) 96
HpaII CCGG 5 cut(s) 106, 233, 328, 334, 339
HphI GGTGA 2 cut(s) 62, 353
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 3 cut(s) 23, 37, 268
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 2 cut(s) 147, 386
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4V TGCA 1 cut(s) 388
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 1 cut(s) 36
Hsp92II CATG 2 cut(s) 103, 392
Kzo9I GATC 3 cut(s) 54, 125, 248
LmnI GCTCC 2 cut(s) 157, 363
LpnPI CCDG 8 cut(s) 119, 136, 246, 341, 346, 347, 352, 373
LweI GCATC 3 cut(s) 14, 182, 210
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 341
MalI GATC 3 cut(s) 56, 127, 250
MboI GATC 3 cut(s) 54, 125, 248
MboII GAAGA 4 cut(s) 10, 74, 160, 168
MflI RGATCY 2 cut(s) 125, 248
MhlI GDGCHC 5 cut(s) 150, 162, 300, 334, 368
MluCI AATT 3 cut(s) 258, 304, 319
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 3 cut(s) 31, 76, 320
MseI TTAA 1 cut(s) 285
MspI CCGG 5 cut(s) 106, 233, 328, 334, 339
MspR9I CCNGG 5 cut(s) 106, 329, 334, 339, 361
MvaI CCWGG 1 cut(s) 361
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 4 cut(s) 106, 329, 334, 339
NdeII GATC 3 cut(s) 54, 125, 248
NlaIII CATG 2 cut(s) 103, 392
NlaIV GGNNCC 4 cut(s) 237, 250, 298, 332
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 1 cut(s) 392
PagI TCATGA 1 cut(s) 99
PceI AGGCCT 1 cut(s) 41
PkrI GCNGC 1 cut(s) 207
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 359
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 4 cut(s) 237, 250, 298, 332
PspOMI GGGCCC 2 cut(s) 296, 330
PspPI GGNCC 6 cut(s) 235, 296, 297, 330, 331, 336
PsuI RGATCY 2 cut(s) 125, 248
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 206
Sau3AI GATC 3 cut(s) 54, 125, 248
Sau96I GGNCC 6 cut(s) 235, 296, 297, 330, 331, 336
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 5 cut(s) 106, 329, 334, 339, 361
SduI GDGCHC 5 cut(s) 150, 162, 300, 334, 368
SetI ASST 3 cut(s) 52, 348, 368
SfaNI GCATC 3 cut(s) 14, 182, 210
Sse9I AATT 3 cut(s) 258, 304, 319
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 2 cut(s) 205, 252
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 368
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 5 cut(s) 104, 327, 332, 337, 359
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 401
TaqI TCGA 3 cut(s) 81, 113, 396
TasI AATT 3 cut(s) 258, 304, 319
TauI GCSGC 1 cut(s) 208
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 1 cut(s) 186
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 2 cut(s) 304, 319
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 357
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.