RLG00000003189

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0021506
Physical Location & Seq
Reverse (-)
45415173 .. 45418273
3101 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003189

Sequence Viewer

Length: 573 bp
ATGGGGAATCTGGAAATCAGGGACCGGTGCCAGAATCTCGATTTCAGAATCAGAGGACGGCGGCATCAGCTACACGCTCAATTTGGAGGACGACTCCACATGCGTCGACGACATCCTCCTCCTGTCGCGGCTGCCTGCCTTTGCCTCCTGGAGTCTAACCCCGCCAAAGAAGTCAACACAAGTCGGCATATTCTGGACGACGTTCTAGAGGAAGATGAGGATAACCGGAGTTGGAGGATGGGATCAATCAATCAAAACTTGGTCCTCCTAGCCCTGCTGGCTCTTGCTGCCCTAGCCATTGCCCCAGCTATCTCCCATGCTCGACTCTCACTCACACCAGAAGATTGCTGGAGTAACATTGACAAAGTGGATGGATGCCAGAAAGAGATTTTCGCATTGTATGGAAAAACTAAAGGTGATATTCACATATATTCTAGTTGCTGCAAAGCCATCCTCGACACCCCGACAAGGTGCAGGATTTGGATTCTCGGGGGCCGAAGGTTTGATCCCGAATTTGCTGCTGGAGTCCTTCAATTTTGTGGTGCAACGAGAATAGTCCCTTCCCCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

191

Amino Acids

21.3

Weight (kDa)

8.63

Isoelectric Point (pI)

62.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 115 - 169 2.5e-06 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021506)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003189
rosa_multiflora Rmu_sc0000532.1_g000004 Rmu_sc0001482.1_g000002
rosa_samantha Rh5DG554200 Rh7AG241900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 106
AccII CGCG 1 cut(s) 128
AciI CCGC 3 cut(s) 61, 128, 162
AclWI GGATC 2 cut(s) 250, 500
AcsI RAATTY 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 468
AgeI ACCGGT 1 cut(s) 24
AgsI TTSAA 1 cut(s) 533
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 2 cut(s) 70, 308
AluI AGCT 2 cut(s) 70, 308
AlwI GGATC 2 cut(s) 250, 500
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 4 cut(s) 131, 287, 441, 518
ApoI RAATTY 1 cut(s) 512
AsiGI ACCGGT 1 cut(s) 24
AspS9I GGNCC 3 cut(s) 22, 262, 493
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 2 cut(s) 22, 262
BanI GGYRCC 1 cut(s) 27
BbvI GCAGC 4 cut(s) 118, 274, 428, 505
BccI CCATC 3 cut(s) 232, 365, 458
BceAI ACGGC 1 cut(s) 74
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 1 cut(s) 149
BfaI CTAG 5 cut(s) 206, 269, 293, 435, 567
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 6 cut(s) 62, 129, 132, 288, 442, 519
BlsI GCNGC 6 cut(s) 63, 130, 133, 289, 443, 520
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 2 cut(s) 22, 262
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 3 cut(s) 22, 262, 493
BmiI GGNNCC 3 cut(s) 23, 29, 494
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 2 cut(s) 73, 365
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BpmI CTGGAG 3 cut(s) 170, 370, 543
BsaWI WCCGGW 2 cut(s) 24, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 468
Bse118I RCCGGY 1 cut(s) 24
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 149
BseGI GGATG 5 cut(s) 112, 243, 376, 380, 450
BseLI CCNNNNNNNGG 1 cut(s) 468
BseMI GCAATG 1 cut(s) 297
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 4 cut(s) 118, 274, 428, 505
BseYI CCCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 27
BshTI ACCGGT 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 2 cut(s) 25, 226
BslFI GGGAC 2 cut(s) 35, 542
BslI CCNNNNNNNGG 1 cut(s) 468
BsmFI GGGAC 2 cut(s) 35, 542
BsnI GGCC 1 cut(s) 495
BsoBI CYCGRG 1 cut(s) 488
Bsp143I GATC 2 cut(s) 242, 505
BspACI CCGC 3 cut(s) 61, 128, 162
BspANI GGCC 1 cut(s) 495
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 3 cut(s) 23, 29, 494
BspPI GGATC 2 cut(s) 250, 500
BspT107I GGYRCC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 24
BssAI RCCGGY 1 cut(s) 24
BssMI GATC 2 cut(s) 242, 505
Bst2UI CCWGG 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 136, 279
BstF5I GGATG 5 cut(s) 112, 243, 376, 380, 450
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 2 cut(s) 245, 508
BstMBI GATC 2 cut(s) 242, 505
BstMWI GCNNNNNNNGC 4 cut(s) 67, 278, 287, 293
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 147
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 118, 274, 428, 505
BsuRI GGCC 1 cut(s) 495
BtsCI GGATG 5 cut(s) 112, 243, 376, 380, 450
Cac8I GCNNGC 2 cut(s) 136, 279
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 3 cut(s) 22, 262, 493
CseI GACGC 1 cut(s) 92
CspAI ACCGGT 1 cut(s) 24
CviAII CATG 2 cut(s) 100, 317
CviJI RGCY 8 cut(s) 70, 131, 272, 281, 296, 308, 449, 495
CviKI_1 RGCY 8 cut(s) 70, 131, 272, 281, 296, 308, 449, 495
DpnI GATC 2 cut(s) 244, 507
DpnII GATC 2 cut(s) 242, 505
Eco47I GGWCC 2 cut(s) 22, 262
Eco88I CYCGRG 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 147
FaeI CATG 2 cut(s) 103, 320
FaiI YATR 6 cut(s) 101, 189, 318, 402, 428, 430
FaqI GGGAC 2 cut(s) 35, 542
FatI CATG 2 cut(s) 99, 316
FauI CCCGC 1 cut(s) 169
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 6 cut(s) 62, 129, 132, 288, 442, 519
FokI GGATG 5 cut(s) 99, 250, 383, 387, 437
Fsp4HI GCNGC 6 cut(s) 62, 129, 132, 288, 442, 519
FspBI CTAG 5 cut(s) 206, 269, 293, 435, 567
GluI GCNGC 6 cut(s) 62, 129, 132, 288, 442, 519
GsaI CCCAGC 1 cut(s) 308
GsuI CTGGAG 3 cut(s) 170, 370, 543
HaeIII GGCC 1 cut(s) 495
HapII CCGG 2 cut(s) 25, 226
HgaI GACGC 1 cut(s) 92
Hin1II CATG 2 cut(s) 103, 320
HincII GTYRAC 2 cut(s) 107, 175
HindII GTYRAC 2 cut(s) 107, 175
HinfI GANTC 8 cut(s) 7, 34, 48, 93, 152, 324, 484, 525
HpaII CCGG 2 cut(s) 25, 226
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 107, 175
Hpy188I TCNGA 2 cut(s) 47, 53
Hpy188III TCNNGA 5 cut(s) 11, 38, 194, 206, 509
Hpy8I GTNNAC 2 cut(s) 107, 175
Hpy99I CGWCG 3 cut(s) 108, 111, 203
HpyAV CCTTC 3 cut(s) 492, 539, 570
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 444, 474, 545
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 278, 287, 293
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 103, 320
Kzo9I GATC 2 cut(s) 242, 505
Lsp1109I GCAGC 4 cut(s) 118, 274, 428, 505
LweI GCATC 2 cut(s) 73, 365
MaeI CTAG 5 cut(s) 206, 269, 293, 435, 567
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 353
MalI GATC 2 cut(s) 244, 507
MboI GATC 2 cut(s) 242, 505
MboII GAAGA 2 cut(s) 224, 353
MluCI AATT 3 cut(s) 80, 512, 533
MlyI GAGTC 4 cut(s) 87, 161, 318, 534
MmeI TCCRAC 1 cut(s) 212
MseI TTAA 1 cut(s) 571
MspI CCGG 2 cut(s) 25, 226
MspR9I CCNGG 1 cut(s) 149
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 67, 278, 287, 293
NdeII GATC 2 cut(s) 242, 505
NlaIII CATG 2 cut(s) 103, 320
NlaIV GGNNCC 3 cut(s) 23, 29, 494
NspI RCATGY 1 cut(s) 103
PfeI GAWTC 4 cut(s) 7, 34, 48, 484
PfoI TCCNGGA 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 24
PkrI GCNGC 6 cut(s) 63, 130, 133, 289, 443, 520
PleI GAGTC 4 cut(s) 87, 160, 318, 533
PpsI GAGTC 4 cut(s) 87, 160, 318, 533
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 3 cut(s) 23, 29, 494
PspPI GGNCC 3 cut(s) 22, 262, 493
SalI GTCGAC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 571
SatI GCNGC 6 cut(s) 62, 129, 132, 288, 442, 519
Sau3AI GATC 2 cut(s) 242, 505
Sau96I GGNCC 3 cut(s) 22, 262, 493
SchI GAGTC 4 cut(s) 87, 161, 318, 534
ScrFI CCNGG 1 cut(s) 149
SetI ASST 6 cut(s) 72, 204, 310, 418, 473, 503
SfaNI GCATC 2 cut(s) 73, 365
SgrDI CGTCGACG 1 cut(s) 105
SinI GGWCC 2 cut(s) 22, 262
Sse9I AATT 3 cut(s) 80, 512, 533
SsiI CCGC 3 cut(s) 61, 128, 162
SspMI CTAG 5 cut(s) 206, 269, 293, 435, 567
StyD4I CCNGG 1 cut(s) 147
TaiI ACGT 1 cut(s) 204
TaqI TCGA 4 cut(s) 39, 106, 322, 456
TasI AATT 3 cut(s) 80, 512, 533
TauI GCSGC 2 cut(s) 64, 131
TfiI GAWTC 4 cut(s) 7, 34, 48, 484
Tru1I TTAA 1 cut(s) 571
Tru9I TTAA 1 cut(s) 571
TseI GCWGC 4 cut(s) 131, 287, 441, 518
VpaK11BI GGWCC 2 cut(s) 22, 262
XapI RAATTY 1 cut(s) 512
XbaI TCTAGA 1 cut(s) 205
XceI RCATGY 1 cut(s) 103
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 106
XspI CTAG 5 cut(s) 206, 269, 293, 435, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.