Rh5DG554200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0021506
Physical Location & Seq
Reverse (-)
85611708 .. 85612025
318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG554200.1

Sequence Viewer

Length: 318 bp
ATGCGTCGACGACATCCTCCTTCGATAGCGGCTGTCGGCCTCTGCCTCCTAGAGTCTGACCCCGCCGGAGAAGTCGACCACGAGCTGGCGTATCTGGACGACATTCTAGAGGAAGATGAGGACAATAGGAGCTGGAGGTCACCGACGGCGAACAATCTGAAGGAGAAGCGTCACCGATCGTTGCGTCGACGGATCTCCTTAGCTAATATCTGCTTCGAATCGGCGTCAAGATCAGAGAACGCGAGAGAAAGAGGAGGAGGTGGTGAAACGATGCCGTCTCGTTTCGCTCCGATCAGCAACAAGGCTTCTCTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

11.79

Weight (kDa)

6.83

Isoelectric Point (pI)

97.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021506)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003189
rosa_multiflora Rmu_sc0000532.1_g000004 Rmu_sc0001482.1_g000002
rosa_samantha Rh5DG554200 Rh7AG241900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 85
AccI GTMKAC 3 cut(s) 7, 75, 187
AccII CGCG 1 cut(s) 242
AciI CCGC 2 cut(s) 29, 63
AclWI GGATC 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 179
AcyI GRCGYC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 85
AluBI AGCT 3 cut(s) 85, 132, 203
AluI AGCT 3 cut(s) 85, 132, 203
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 37
AsuHPI GGTGA 3 cut(s) 132, 164, 275
AsuII TTCGAA 1 cut(s) 216
BauI CACGAG 1 cut(s) 80
BceAI ACGGC 2 cut(s) 162, 259
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 2 cut(s) 50, 107
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmsI GCATC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 154
Bpu10I CCTNAGC 1 cut(s) 199
Bpu14I TTCGAA 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 85
BseGI GGATG 1 cut(s) 13
BseLI CCNNNNNNNGG 1 cut(s) 85
BseRI GAGGAG 2 cut(s) 267, 270
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 179
BsiSI CCGG 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 282
BsmBI CGTCTC 1 cut(s) 282
BsnI GGCC 1 cut(s) 39
Bsp119I TTCGAA 1 cut(s) 216
Bsp143I GATC 4 cut(s) 176, 192, 230, 291
BspACI CCGC 2 cut(s) 29, 63
BspANI GGCC 1 cut(s) 39
BspFNI CGCG 1 cut(s) 242
BspPI GGATC 1 cut(s) 200
BspT104I TTCGAA 1 cut(s) 216
BssMI GATC 4 cut(s) 176, 192, 230, 291
BssNI GRCGYC 1 cut(s) 224
BssSI CACGAG 1 cut(s) 80
Bst2BI CACGAG 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 199
BstEII GGTNACC 1 cut(s) 138
BstF5I GGATG 1 cut(s) 13
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 4 cut(s) 179, 195, 233, 294
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 4 cut(s) 176, 192, 230, 291
BstMCI CGRYCG 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 138
BstUI CGCG 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 1 cut(s) 39
BtsCI GGATG 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 87
CseI GACGC 3 cut(s) 158, 173, 213
CviJI RGCY 6 cut(s) 32, 39, 85, 132, 203, 305
CviKI_1 RGCY 6 cut(s) 32, 39, 85, 132, 203, 305
DdeI CTNAG 1 cut(s) 199
DpnI GATC 4 cut(s) 178, 194, 232, 293
DpnII GATC 4 cut(s) 176, 192, 230, 291
Eco57I CTGAAG 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 138
EcoO65I GGTNACC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 282
FauI CCCGC 1 cut(s) 70
FblI GTMKAC 3 cut(s) 7, 75, 187
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 2 cut(s) 50, 107
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 154
HaeIII GGCC 1 cut(s) 39
HapII CCGG 1 cut(s) 66
HgaI GACGC 3 cut(s) 158, 173, 213
Hin1I GRCGYC 1 cut(s) 224
HincII GTYRAC 3 cut(s) 8, 76, 188
HindII GTYRAC 3 cut(s) 8, 76, 188
HinfI GANTC 2 cut(s) 53, 218
HpaII CCGG 1 cut(s) 66
HphI GGTGA 3 cut(s) 132, 164, 275
Hpy166II GTNNAC 3 cut(s) 8, 76, 188
Hpy188I TCNGA 4 cut(s) 58, 159, 235, 291
Hpy188III TCNNGA 3 cut(s) 95, 107, 228
Hpy8I GTNNAC 3 cut(s) 8, 76, 188
Hpy99I CGWCG 5 cut(s) 9, 12, 148, 189, 192
HpyAV CCTTC 2 cut(s) 30, 154
HpyF3I CTNAG 1 cut(s) 199
Hsp92I GRCGYC 1 cut(s) 224
Kzo9I GATC 4 cut(s) 176, 192, 230, 291
LmnI GCTCC 2 cut(s) 129, 292
LpnPI CCDG 4 cut(s) 71, 79, 80, 118
LweI GCATC 1 cut(s) 261
MaeI CTAG 2 cut(s) 50, 107
MaeIII GTNAC 2 cut(s) 138, 170
MalI GATC 4 cut(s) 178, 194, 232, 293
MboI GATC 4 cut(s) 176, 192, 230, 291
MboII GAAGA 1 cut(s) 125
MflI RGATCY 1 cut(s) 192
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 9 cut(s) 27, 50, 56, 103, 112, 129, 245, 248, 251
MspI CCGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 242
NdeII GATC 4 cut(s) 176, 192, 230, 291
NmuCI GTSAC 2 cut(s) 138, 170
NspV TTCGAA 1 cut(s) 216
PfeI GAWTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 85
PkrI GCNGC 1 cut(s) 31
Ple19I CGATCG 1 cut(s) 179
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
PspEI GGTNACC 1 cut(s) 138
PsuI RGATCY 1 cut(s) 192
PvuI CGATCG 1 cut(s) 179
SalI GTCGAC 3 cut(s) 6, 74, 186
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 4 cut(s) 176, 192, 230, 291
SchI GAGTC 1 cut(s) 62
SetI ASST 5 cut(s) 87, 134, 140, 205, 262
SfaNI GCATC 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 216
SgrDI CGTCGACG 2 cut(s) 6, 186
SsiI CCGC 2 cut(s) 29, 63
SspMI CTAG 2 cut(s) 50, 107
TaqI TCGA 5 cut(s) 7, 23, 75, 187, 216
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 1 cut(s) 218
TseFI GTSAC 2 cut(s) 138, 170
Tsp45I GTSAC 2 cut(s) 138, 170
TspGWI ACGGA 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 85
XbaI TCTAGA 1 cut(s) 106
XmiI GTMKAC 3 cut(s) 7, 75, 187
XspI CTAG 2 cut(s) 50, 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.